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romrgfp his6 synthetic coding sequences  (Twist Bioscience)


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    Structured Review

    Twist Bioscience romrgfp his6 synthetic coding sequences
    Romrgfp His6 Synthetic Coding Sequences, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/code+no/pet28a/pm42297786-293-7-18
    Average 86 stars, based on 1 article reviews
    romrgfp his6 synthetic coding sequences - by Bioz Stars, 2026-10
    86/100 stars

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    Isolation:

    Article Title: Machine learning-driven optimization of specific, compact, and efficient base editors via single-round diversification.
    Article Snippet: .. The digests were then pooled and fragments from 75 to 300 bp, representing approximately half of all the digestion products with no fragment being > 65% of the size of the initial synthesis product, were isolated via gel electrophoresis and assembled via polymerase chain reaction (PCR) into full-length deaminase constructs, which were isolated via gel electrophoresis and cloned into acceptor pET28 vectors synthesized by Twist Biosciences. .. These starting pools then individually underwent DNA shuffling [ 34 ] by being separately digested with eight different four-base-pair restriction enzymes (AluI, HaeIII, HhaI, HinfI, HpaII, MboI, RsaI, and TaqI) from New England Biolabs according to the manufacturer’s specifications ( Supplementary Fig. S6 ).

    Nucleic Acid Electrophoresis:

    Article Title: Machine learning-driven optimization of specific, compact, and efficient base editors via single-round diversification.
    Article Snippet: .. The digests were then pooled and fragments from 75 to 300 bp, representing approximately half of all the digestion products with no fragment being > 65% of the size of the initial synthesis product, were isolated via gel electrophoresis and assembled via polymerase chain reaction (PCR) into full-length deaminase constructs, which were isolated via gel electrophoresis and cloned into acceptor pET28 vectors synthesized by Twist Biosciences. .. These starting pools then individually underwent DNA shuffling [ 34 ] by being separately digested with eight different four-base-pair restriction enzymes (AluI, HaeIII, HhaI, HinfI, HpaII, MboI, RsaI, and TaqI) from New England Biolabs according to the manufacturer’s specifications ( Supplementary Fig. S6 ).

    Polymerase Chain Reaction:

    Article Title: Machine learning-driven optimization of specific, compact, and efficient base editors via single-round diversification.
    Article Snippet: .. The digests were then pooled and fragments from 75 to 300 bp, representing approximately half of all the digestion products with no fragment being > 65% of the size of the initial synthesis product, were isolated via gel electrophoresis and assembled via polymerase chain reaction (PCR) into full-length deaminase constructs, which were isolated via gel electrophoresis and cloned into acceptor pET28 vectors synthesized by Twist Biosciences. .. These starting pools then individually underwent DNA shuffling [ 34 ] by being separately digested with eight different four-base-pair restriction enzymes (AluI, HaeIII, HhaI, HinfI, HpaII, MboI, RsaI, and TaqI) from New England Biolabs according to the manufacturer’s specifications ( Supplementary Fig. S6 ).

    Construct:

    Article Title: Machine learning-driven optimization of specific, compact, and efficient base editors via single-round diversification.
    Article Snippet: .. The digests were then pooled and fragments from 75 to 300 bp, representing approximately half of all the digestion products with no fragment being > 65% of the size of the initial synthesis product, were isolated via gel electrophoresis and assembled via polymerase chain reaction (PCR) into full-length deaminase constructs, which were isolated via gel electrophoresis and cloned into acceptor pET28 vectors synthesized by Twist Biosciences. .. These starting pools then individually underwent DNA shuffling [ 34 ] by being separately digested with eight different four-base-pair restriction enzymes (AluI, HaeIII, HhaI, HinfI, HpaII, MboI, RsaI, and TaqI) from New England Biolabs according to the manufacturer’s specifications ( Supplementary Fig. S6 ).

    Clone Assay:

    Article Title: Machine learning-driven optimization of specific, compact, and efficient base editors via single-round diversification.
    Article Snippet: .. The digests were then pooled and fragments from 75 to 300 bp, representing approximately half of all the digestion products with no fragment being > 65% of the size of the initial synthesis product, were isolated via gel electrophoresis and assembled via polymerase chain reaction (PCR) into full-length deaminase constructs, which were isolated via gel electrophoresis and cloned into acceptor pET28 vectors synthesized by Twist Biosciences. .. These starting pools then individually underwent DNA shuffling [ 34 ] by being separately digested with eight different four-base-pair restriction enzymes (AluI, HaeIII, HhaI, HinfI, HpaII, MboI, RsaI, and TaqI) from New England Biolabs according to the manufacturer’s specifications ( Supplementary Fig. S6 ).

    Article Title: Cell polarity control by an unconventional G-protein complex in bacteria.
    Article Snippet: Fractions containing the protein were pooled, concentrated, dialysed overnight in Buffer D (Buffer A without imidazole) and stored at -80°C in Buffer D. Protein purity was analyzed by SDS-PAGE, and protein concentrations were quantified using NanoDrop (Thermo Scientific). .. - Expression and purification of RomR-His6 and RomRGFP-His6 Synthetic coding sequences of RomR and RomRGFP were obtained from Twist Bioscience, cloned in a modified pET28 vector for expression of C-terminal hexa-histidine tagged proteins. .. - Expression and purification of RomR-His6 and RomRGFP-His6 Synthetic coding sequences of RomR and RomRGFP were obtained from Twist Bioscience, cloned in a modified pET28 vector for expression of C-terminal hexa-histidine tagged proteins.

    Article Title: Large-scale discovery, analysis and design of protein energy landscapes.
    Article Snippet: .. The DNA sequences were codon-optimized for E. coli and cloned into pET-28a(+) (thrombin cleavage site) from Twist Biosciences or pET-28a(+)-TEV from GenScript. ..

    Article Title: Nucleoprotein and glycoprotein based serological assays for detection of Marburg virus infections
    Article Snippet: .. These sequences were codon-optimised for bacterial expression and cloned into pET28a by Twist Bioscience. ..

    Synthesized:

    Article Title: Machine learning-driven optimization of specific, compact, and efficient base editors via single-round diversification.
    Article Snippet: .. The digests were then pooled and fragments from 75 to 300 bp, representing approximately half of all the digestion products with no fragment being > 65% of the size of the initial synthesis product, were isolated via gel electrophoresis and assembled via polymerase chain reaction (PCR) into full-length deaminase constructs, which were isolated via gel electrophoresis and cloned into acceptor pET28 vectors synthesized by Twist Biosciences. .. These starting pools then individually underwent DNA shuffling [ 34 ] by being separately digested with eight different four-base-pair restriction enzymes (AluI, HaeIII, HhaI, HinfI, HpaII, MboI, RsaI, and TaqI) from New England Biolabs according to the manufacturer’s specifications ( Supplementary Fig. S6 ).

    Article Title: Structural basis of iron piracy by human gut Bacteroides
    Article Snippet: .. Similarly, the nucleotide sequence encoding amino acid residues 29-491 of B. viscericola DSM 18177 XusB ( BARVI_05925 ) was synthesized by Twist Bioscience and subcloned into pET28b using NcoI and XhoI restriction sites. ..

    Article Title: Structural analysis of de novo designed binders targeting the closed state of HSP90
    Article Snippet: .. For the 31 selected binders, genes were synthesized in pET28a vector by TwistBioscience. .. For the 31 selected binders, genes were synthesized in pET28a vector by TwistBioscience.

    other:

    Article Title: De novo and scaffold-based design of GDF15 binders for cancer cachexia diagnostics and therapeutics.
    Article Snippet: The gene encoding mature human GDF15 (residues 198–308; GenBank accession no. 9518) was inserted into the NotI and XhoI sites of a modified pcDNA3.4 vector (#A14697, Invitrogen) containing an N-terminal Twin-Strep tag followed by a thrombin cleavage sequence.

    Expressing:

    Article Title: Cell polarity control by an unconventional G-protein complex in bacteria.
    Article Snippet: Fractions containing the protein were pooled, concentrated, dialysed overnight in Buffer D (Buffer A without imidazole) and stored at -80°C in Buffer D. Protein purity was analyzed by SDS-PAGE, and protein concentrations were quantified using NanoDrop (Thermo Scientific). .. - Expression and purification of RomR-His6 and RomRGFP-His6 Synthetic coding sequences of RomR and RomRGFP were obtained from Twist Bioscience, cloned in a modified pET28 vector for expression of C-terminal hexa-histidine tagged proteins. .. - Expression and purification of RomR-His6 and RomRGFP-His6 Synthetic coding sequences of RomR and RomRGFP were obtained from Twist Bioscience, cloned in a modified pET28 vector for expression of C-terminal hexa-histidine tagged proteins.

    Article Title: Nucleoprotein and glycoprotein based serological assays for detection of Marburg virus infections
    Article Snippet: .. These sequences were codon-optimised for bacterial expression and cloned into pET28a by Twist Bioscience. ..

    Purification:

    Article Title: Cell polarity control by an unconventional G-protein complex in bacteria.
    Article Snippet: Fractions containing the protein were pooled, concentrated, dialysed overnight in Buffer D (Buffer A without imidazole) and stored at -80°C in Buffer D. Protein purity was analyzed by SDS-PAGE, and protein concentrations were quantified using NanoDrop (Thermo Scientific). .. - Expression and purification of RomR-His6 and RomRGFP-His6 Synthetic coding sequences of RomR and RomRGFP were obtained from Twist Bioscience, cloned in a modified pET28 vector for expression of C-terminal hexa-histidine tagged proteins. .. - Expression and purification of RomR-His6 and RomRGFP-His6 Synthetic coding sequences of RomR and RomRGFP were obtained from Twist Bioscience, cloned in a modified pET28 vector for expression of C-terminal hexa-histidine tagged proteins.

    Modification:

    Article Title: Cell polarity control by an unconventional G-protein complex in bacteria.
    Article Snippet: Fractions containing the protein were pooled, concentrated, dialysed overnight in Buffer D (Buffer A without imidazole) and stored at -80°C in Buffer D. Protein purity was analyzed by SDS-PAGE, and protein concentrations were quantified using NanoDrop (Thermo Scientific). .. - Expression and purification of RomR-His6 and RomRGFP-His6 Synthetic coding sequences of RomR and RomRGFP were obtained from Twist Bioscience, cloned in a modified pET28 vector for expression of C-terminal hexa-histidine tagged proteins. .. - Expression and purification of RomR-His6 and RomRGFP-His6 Synthetic coding sequences of RomR and RomRGFP were obtained from Twist Bioscience, cloned in a modified pET28 vector for expression of C-terminal hexa-histidine tagged proteins.

    Sequencing:

    Article Title: Structural basis of iron piracy by human gut Bacteroides
    Article Snippet: .. Similarly, the nucleotide sequence encoding amino acid residues 29-491 of B. viscericola DSM 18177 XusB ( BARVI_05925 ) was synthesized by Twist Bioscience and subcloned into pET28b using NcoI and XhoI restriction sites. ..

    Plasmid Preparation:

    Article Title: Structural analysis of de novo designed binders targeting the closed state of HSP90
    Article Snippet: .. For the 31 selected binders, genes were synthesized in pET28a vector by TwistBioscience. .. For the 31 selected binders, genes were synthesized in pET28a vector by TwistBioscience.



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    (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli <t>K12</t> (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .
    Molecule Array Simoa Neurology 4 Plex E Neurology 4 Plex E N4pe Advantage Plus Assay, supplied by Quanterix, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli <t>K12</t> (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .
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    (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli <t>K12</t> (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .
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    (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli <t>K12</t> (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .
    R Code For Bayesian Spatiotemporal And Regression Analyses, supplied by Mendeley Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli <t>K12</t> (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .
    Zym D Invivogen Cat Code Tlrl Zyd, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli <t>K12</t> (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .
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    (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli K12 (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .

    Journal: PLOS Biology

    Article Title: Uracil-driven ROS signaling and larval TrpA1-B neuron activation are required for priming the adult Drosophila gustatory response to bacteria

    doi: 10.1371/journal.pbio.3004042

    Figure Lengend Snippet: (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli K12 (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .

    Article Snippet: PGN is E. coli K12: Invivogen, catalog code #tlrl-kipgn.

    Techniques: Bacteria, Control, RNAi Assay, Activity Assay, Inactivation, Expressing, Concentration Assay