romrgfp his6 synthetic coding sequences (Twist Bioscience)
Structured Review
Romrgfp His6 Synthetic Coding Sequences, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/code+no/pet28a/pm42297786-293-7-18
Average 86 stars, based on 1 article reviews
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Isolation:Article Title: Machine learning-driven optimization of specific, compact, and efficient base editors via single-round diversification. Article Snippet: .. The digests were then pooled and fragments from 75 to 300 bp, representing approximately half of all the digestion products with no fragment being > 65% of the size of the initial synthesis product, were isolated via gel electrophoresis and assembled via polymerase chain reaction (PCR) into full-length deaminase constructs, which were isolated via gel electrophoresis and cloned into Nucleic Acid Electrophoresis:Article Title: Machine learning-driven optimization of specific, compact, and efficient base editors via single-round diversification. Article Snippet: .. The digests were then pooled and fragments from 75 to 300 bp, representing approximately half of all the digestion products with no fragment being > 65% of the size of the initial synthesis product, were isolated via gel electrophoresis and assembled via polymerase chain reaction (PCR) into full-length deaminase constructs, which were isolated via gel electrophoresis and cloned into Polymerase Chain Reaction:Article Title: Machine learning-driven optimization of specific, compact, and efficient base editors via single-round diversification. Article Snippet: .. The digests were then pooled and fragments from 75 to 300 bp, representing approximately half of all the digestion products with no fragment being > 65% of the size of the initial synthesis product, were isolated via gel electrophoresis and assembled via polymerase chain reaction (PCR) into full-length deaminase constructs, which were isolated via gel electrophoresis and cloned into Construct:Article Title: Machine learning-driven optimization of specific, compact, and efficient base editors via single-round diversification. Article Snippet: .. The digests were then pooled and fragments from 75 to 300 bp, representing approximately half of all the digestion products with no fragment being > 65% of the size of the initial synthesis product, were isolated via gel electrophoresis and assembled via polymerase chain reaction (PCR) into full-length deaminase constructs, which were isolated via gel electrophoresis and cloned into Clone Assay:Article Title: Machine learning-driven optimization of specific, compact, and efficient base editors via single-round diversification. Article Snippet: .. The digests were then pooled and fragments from 75 to 300 bp, representing approximately half of all the digestion products with no fragment being > 65% of the size of the initial synthesis product, were isolated via gel electrophoresis and assembled via polymerase chain reaction (PCR) into full-length deaminase constructs, which were isolated via gel electrophoresis and cloned into Article Title: Cell polarity control by an unconventional G-protein complex in bacteria. Article Snippet: Fractions containing the protein were pooled, concentrated, dialysed overnight in Buffer D (Buffer A without imidazole) and stored at -80°C in Buffer D. Protein purity was analyzed by SDS-PAGE, and protein concentrations were quantified using NanoDrop (Thermo Scientific). .. - Expression and purification of RomR-His6 and Article Title: Large-scale discovery, analysis and design of protein energy landscapes. Article Snippet: .. The DNA sequences were codon-optimized for E. coli and cloned into pET-28a( Article Title: Nucleoprotein and glycoprotein based serological assays for detection of Marburg virus infections Article Snippet: .. These sequences were codon-optimised for bacterial expression and cloned into Synthesized:Article Title: Machine learning-driven optimization of specific, compact, and efficient base editors via single-round diversification. Article Snippet: .. The digests were then pooled and fragments from 75 to 300 bp, representing approximately half of all the digestion products with no fragment being > 65% of the size of the initial synthesis product, were isolated via gel electrophoresis and assembled via polymerase chain reaction (PCR) into full-length deaminase constructs, which were isolated via gel electrophoresis and cloned into Article Title: Structural basis of iron piracy by human gut Bacteroides Article Snippet: .. Similarly, the nucleotide sequence encoding amino acid residues 29-491 of B. viscericola DSM 18177 XusB ( BARVI_05925 ) was synthesized by Twist Bioscience and subcloned into Article Title: Structural analysis of de novo designed binders targeting the closed state of HSP90 Article Snippet: .. For the 31 selected binders, genes were synthesized in other:Article Title: De novo and scaffold-based design of GDF15 binders for cancer cachexia diagnostics and therapeutics. Article Snippet: The gene encoding mature human GDF15 (residues 198–308; GenBank accession no. 9518) was inserted into the NotI and XhoI sites of a modified pcDNA3.4 vector (#A14697, Invitrogen) containing an N-terminal Twin-Strep tag followed by a thrombin cleavage sequence. Expressing:Article Title: Cell polarity control by an unconventional G-protein complex in bacteria. Article Snippet: Fractions containing the protein were pooled, concentrated, dialysed overnight in Buffer D (Buffer A without imidazole) and stored at -80°C in Buffer D. Protein purity was analyzed by SDS-PAGE, and protein concentrations were quantified using NanoDrop (Thermo Scientific). .. - Expression and purification of RomR-His6 and Article Title: Nucleoprotein and glycoprotein based serological assays for detection of Marburg virus infections Article Snippet: .. These sequences were codon-optimised for bacterial expression and cloned into Purification:Article Title: Cell polarity control by an unconventional G-protein complex in bacteria. Article Snippet: Fractions containing the protein were pooled, concentrated, dialysed overnight in Buffer D (Buffer A without imidazole) and stored at -80°C in Buffer D. Protein purity was analyzed by SDS-PAGE, and protein concentrations were quantified using NanoDrop (Thermo Scientific). .. - Expression and purification of RomR-His6 and Modification:Article Title: Cell polarity control by an unconventional G-protein complex in bacteria. Article Snippet: Fractions containing the protein were pooled, concentrated, dialysed overnight in Buffer D (Buffer A without imidazole) and stored at -80°C in Buffer D. Protein purity was analyzed by SDS-PAGE, and protein concentrations were quantified using NanoDrop (Thermo Scientific). .. - Expression and purification of RomR-His6 and Sequencing:Article Title: Structural basis of iron piracy by human gut Bacteroides Article Snippet: .. Similarly, the nucleotide sequence encoding amino acid residues 29-491 of B. viscericola DSM 18177 XusB ( BARVI_05925 ) was synthesized by Twist Bioscience and subcloned into Plasmid Preparation:Article Title: Structural analysis of de novo designed binders targeting the closed state of HSP90 Article Snippet: .. For the 31 selected binders, genes were synthesized in |
