code availability d experimental model (Proteintech)

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Proteintech code availability d experimental model
Code Availability D Experimental Model, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/code+availability+d+experimental+model/CEL+Antibody/pm37979585-200-32-72
Average 96 stars, based on 2375 article reviews

code availability d experimental model - by Bioz Stars, 2026-10

96/100 stars

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Incubation:

Article Title: Monochromatic light affects intestinal barrier function of chick via an anti-oxidation pathway involving melatonin and its receptor Mel1b
Article Snippet: .. Paraffin jejunal sections were incubated with primary antibodies at 4°C overnight: proliferating cell nuclear antigen (PCNA, 1:300; Proteintech, Wuhan, China), IgA (1:300; Abcam, Cambridge, England), Mel 1a (1:200; Abmart, Shanghai, China), Mel 1b (1:300; Abcam, USA) and Mel 1c (1:100; Abmart, Shanghai, China). .. Then, the sections were rinsed with PBS (0.01 M, pH = 7.4) and incubated with biotinylated secondary antibodies (Goat-anti-Mouse/Goat-anti-Rabbit, 1:300, CWBio, Taizhou, China) and streptavidin-horseradish peroxidase (1:300, Vector Laboratories, Burlingame, CA, USA) successively for 2 h at room temperature.

Article Title: A paracrine-to-autocrine shunt of GREM1 fuels colorectal cancer metastasis via ACVR1C.
Article Snippet: .. Subsequently, the samples were incubated with goat anti-GREM1 (3 μg/mL, R&D, AF956), mouse anti-β-Catenin (1:100, BD, 610154), rabbit anti-β-Catenin (1:100, Absea, RC-6352), rabbit anti-Vimentin (1:100, CST, 5741), rabbit anti-CD68 (1:100, CST, 26042), rabbit anti-FAP (1:50, Proteintech, 15384-1-AP), rabbit anti-α-SMA (1:100, Abcam, ab5694), rabbit anti-E-Cadherin (1:200, CST, 3195), rabbit antiACVR1C (1:50, Thermo, PA587475), rabbit anti-Snail (1:200, Abcam, ab224731) and mouse anti-CDX2 (1:200, Servicebio, GB151501) overnight at 4 °C. .. The tissues were incubated with Alexa-Fluor-conjugated secondary antibodies (Invitrogen) in AR TIC LE IN PR ES S PBS with 1 % normal donkey serum for 1 h at room temperature.

Article Title: Islet regeneration protein Reg3g promotes macrophage clearance of β cell-derived dysfunctional mitochondria-rich vesicles to mitigate T2DM
Article Snippet: .. And then cell slides were incubated with primary antibodies, including LC3A/B (#12741, CST), and P2RX7(28207-1-AP, Proteintech) at 4 °C overnight. ..

Article Title: Remote Pancreatic Response to Severe Polytrauma Correlates With Clinical Outcome
Article Snippet: After UV activation using a Chemidoc XRS+ (Biorad), proteins were blotted onto a PVDF membrane and total lane protein was measured using the Chemidoc imager. .. Membranes were blocked with 5% BSA in 0.1% Tween20 (Sigma, Taufkirchen, Germany) in TBS (TBS‐T) for 1 h at 4°C, and incubated with anti‐Cel (Proteintech, 15384‐1‐AP, 1:2000) and anti‐Pnlip (Proteintech, 11209‐1‐AP, 1:2000) antibodies o/n at 4°C. .. After washing three times with TBS‐T, 1:5000 ECL anti‐rabbit horseradish peroxidase (HRP) IgG secondary antibody (GE Healthcare, Duesseldorf, Germany) was added for 1 h at RT.

Cell Culture:

Article Title: The sphingolipid metabolite sphingosine protects against hypertension by targeting metabolic-inflammatory crosstalk via the NLRP3 inflammasome
Article Snippet: .. The concentrations of secreted proinflammatory cytokines in the cell culture supernatant were quantified using commercial ELISA kits for human IL-1β (Proteintech, Cat# KE00021) and human IL-18 (Proteintech, Cat# KE00193), following the manufacturer's instructions. ..

Enzyme-linked Immunosorbent Assay:

Purification:

Article Title: E2F1 K117 methylation by SETD6 disrupts BRD4–E2F1 binding and modulates E2F1 chromatin binding and gene regulation in prostate cancer cells
Article Snippet: Next, cells were washed with 1× Phosphate Buffered Saline (PBS) buffer (Gibco) and harvested by centrifugation at 500 g for 5 min. After harvesting, the amount of proteins in whole cell lysate was determined by western-blot. .. For protein/protein interaction analysis, the GFP-fused proteins were purified from the cell extract using GFP-trap A beads (Chromotek) according to the manufacturer’s instructions. ..

Transcranial Magnetic Stimulation:

Blocking Assay:

Article Title: TLR2-PI3K/Akt mediated microbe-mimetic priming boosts the therapeutic paracrine function of GelMA-Encapsulated MSCs for diabetic wound regeneration
Article Snippet: After being centrifuged, quantified and denatured, 80 μg protein samples were separated by 12 % sodium dodecyl sulfate polyacrylamide gel electrophoresis and were then transferred onto methanol-activated polyvinylidene difluoride membranes (GE Healthcare, USA). .. Bovine serum albumin solutions (5 %) were used to block non-specific antigens for 1.5 h. The membranes were probed with anti-PI3K rabbit monoclonal antibody (1:500, C67E7, Cell siginting), anti-Phospho-Akt (Ser473) rabbit monoclonal antibody (1:500, 9271T, Cell siginting), anti- P13K rabbit monoclonal antibody (1:500, C73F8, Cell siginting), anti-p-PI3K rabbit monoclonal antibody (1:500, ab278545, Abcam) or anti-GAPDH mouse monoclonal antibody (1:1000, 60 004–1-Ig, Proteintech). .. Protein bands were visualized using a UVITEC Alliance MINI HD9 instrument (UVITEC, UK) with an enhanced chemiluminescence detection system.

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