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legendplextm cloud-based data analysis software  (Qognit Inc)

 
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    Qognit Inc legendplextm cloud-based data analysis software
    Legendplextm Cloud Based Data Analysis Software, supplied by Qognit Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cloud-based+data+analysis+software/legendplextm+data+analysis+software+suite/pm40600334-54-15-20
    Average 90 stars, based on 1 article reviews
    legendplextm cloud-based data analysis software - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Software:

    Article Title: Mitochondrial DNA variants and microbiota: An experimental strategy to identify novel therapeutic potential in chronic inflammatory diseases.
    Article Snippet: A LEGENDplexTM Mouse Inflammation Panel (13-plex) with a V-bottom Panel (Biolegend) was used to determine cytokine levels in the mouse serum samples. .. The FCS files were analysed using the LEGENDplexTM Data Analysis Software Suite (https://legendplex.qognit.com/user/login?next=home). .. GraphPad Prism v10 (GraphPad Software, Boston MA, USA) was used for statistical analyses (except the shotgun metagenomics sequencing data analysis).

    Article Title: Regulatory T Cell Phenotype Related to Cytokine Expression Patterns in Post-COVID-19 Pulmonary Fibrosis and Idiopathic Pulmonary Fibrosis.
    Article Snippet: Reactions were run in duplicate using a BD FACSLyric flow cytometer (BD‐Biosciences San Jose, CA, USA). .. Data analysis was done using the LEGENDplexTM Data Analysis Software Suite (QOGNIT). ..

    Article Title: Increased Herpesvirus Entry Mediator Expression on Circulating Monocytes and Subsets Predicts Poor Outcomes in Pancreatic Ductal Adenocarcinoma Patients.
    Article Snippet: .. Data acquisition was performed on flow cytometer and analyzed with the LEGENDPlexTM Data Analysis Software Suite (https://legendplex.qognit.com/workflow/171050 (accessed on 17 March 2025), Biolegend, San Diego, CA, USA). .. Data were stored on a laboratory computer and analyzed using GraphPad Prism (Version 9.5.1 (733) for Windows, GraphPad Software, Boston, MA, USA).

    Article Title: An effective approach to modulate mitochondrial function in murine primary macrophages by a mitochondria-targeted nanocapsule, MITO-Porter.
    Article Snippet: Macrophages play crucial roles in various pathological conditions as well as maintenance of homeostasis.. Many chronic inflammatory conditions, such as atherosclerosis, rheumatoid arthritis, and obesity, are known to involve the polarization of macrophages into a proinflammatory state.. Therefore, controlling the function of macrophages is a potential strategy to intervene in such pathological conditions.

    other:

    Article Title: Tirzepatide, a dual GIP/GLP1-receptor co-agonist preserves cardiac function and improves survival in angiotensin II-induced heart failure model in mice: comparison to liraglutide.
    Article Snippet: Data analysis was carried out by a blinded method using the LEGENDplexTM qognit cloud-based software and manually in GraphPad Prism software (version 8.0.1).

    Article Title: Contribution of parasite and host genotype to immunopathology of schistosome infections.
    Article Snippet: The analysis was conducted using LEGENDplexTM Cloud-based Data Analysis Software (https:// legen dplex. qognit. com/).

    Article Title: Analysis of human colostrum reveals differential co-occurrence networks of metabolites, microbiota and cytokines in maternal obesity.
    Article Snippet: Data analysis was performed using BD software, which is accessible online at the website of LEGENDplexTM cloud-based data analysis software (https://legendplex.qognit.com).

    Flow Cytometry:

    Article Title: Increased Herpesvirus Entry Mediator Expression on Circulating Monocytes and Subsets Predicts Poor Outcomes in Pancreatic Ductal Adenocarcinoma Patients.
    Article Snippet: .. Data acquisition was performed on flow cytometer and analyzed with the LEGENDPlexTM Data Analysis Software Suite (https://legendplex.qognit.com/workflow/171050 (accessed on 17 March 2025), Biolegend, San Diego, CA, USA). .. Data were stored on a laboratory computer and analyzed using GraphPad Prism (Version 9.5.1 (733) for Windows, GraphPad Software, Boston, MA, USA).

    Incubation:

    Article Title: MiRNA Let-7i-5p-Contained Small Extracellular Vesicles from Macrophages Induce Nucleus Pulposus Cell Senescence via Targeting LIN28A
    Article Snippet: .. After being washed with washing buffer, the beads were incubated with 25 μL detection antibody for 2 h and 25 μL SA-PE for 1 h. The fluorescence intensity of the beads was detected by FCM and analyzed using the LEGENDplexTM QOGNIT Software. ..

    Fluorescence:

    Article Title: MiRNA Let-7i-5p-Contained Small Extracellular Vesicles from Macrophages Induce Nucleus Pulposus Cell Senescence via Targeting LIN28A
    Article Snippet: .. After being washed with washing buffer, the beads were incubated with 25 μL detection antibody for 2 h and 25 μL SA-PE for 1 h. The fluorescence intensity of the beads was detected by FCM and analyzed using the LEGENDplexTM QOGNIT Software. ..



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    Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by <t>LEGENDplex.</t> Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.
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    Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by <t>LEGENDplex.</t> Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.
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    Image Search Results


    Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by LEGENDplex. Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.

    Journal: Science immunology

    Article Title: Human LFA-1 governs T cell immune surveillance of the skin

    doi: 10.1126/sciimmunol.adz8360

    Figure Lengend Snippet: Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by LEGENDplex. Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.

    Article Snippet: Data were analyzed with LEGENDplex Cloud-based Data Analysis Software (Qognit).

    Techniques: Flow Cytometry, MANN-WHITNEY, Immunopeptidomics