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the jpk data processing tool  (JPK Instruments AG)

 
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    JPK Instruments AG the jpk data processing tool
    Heterotypic aggregation: in-vitro coaggregation of OTUB1 and α-Synuclein. A and B , seeding effects of OTUB1 aggregates on the aggregation of α-Synuclein were studied by using ThT binding kinetics ( A ) and atomic force microscopy ( B ). OTUB1 seeds (5 μM) were added at 0 h to 300 μM of α-Synuclein and aggregation kinetics were performed using ThT dye binding assay. Aliquotes at different time intervals were collected to measure ThT fluorescence and for AFM sample preparation. The ThT data was plotted in GraphPad Prism and fitted using a non-linear fit sigmoidal model, and subjected to a two-way ANOVA (Dunnett’s multiple comparison test) to assess statistical significance. ∗∗∗∗ denotes a p -value of <0.0001. All the AFM samples were prepared on freshly cleaved mica surfaces, and imaging was performed in intermittent tapping mode <t>using</t> <t>Bruker</t> NanoWizard 3 <t>(JPK</t> instruments); data was processed using the JPK data processing tool (scale bar- 1 μm). C , cross-seeding assay by sonicated α-Synuclein pre-formed fibrils shows they can nucleate wild-type ( violet ) but not the mutants F133A (black) and V173K ( orange ) as observed from the light scattering assay.
    The Jpk Data Processing Tool, supplied by JPK Instruments AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cloud+data+processing+tool/the+jpk+data+processing+tool/pmc11154711-251-30-23
    Average 90 stars, based on 1 article reviews
    the jpk data processing tool - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Hotspot site microenvironment in the deubiquitinase OTUB1 drives its stability and aggregation"

    Article Title: Hotspot site microenvironment in the deubiquitinase OTUB1 drives its stability and aggregation

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2024.107315

    Heterotypic aggregation: in-vitro coaggregation of OTUB1 and α-Synuclein. A and B , seeding effects of OTUB1 aggregates on the aggregation of α-Synuclein were studied by using ThT binding kinetics ( A ) and atomic force microscopy ( B ). OTUB1 seeds (5 μM) were added at 0 h to 300 μM of α-Synuclein and aggregation kinetics were performed using ThT dye binding assay. Aliquotes at different time intervals were collected to measure ThT fluorescence and for AFM sample preparation. The ThT data was plotted in GraphPad Prism and fitted using a non-linear fit sigmoidal model, and subjected to a two-way ANOVA (Dunnett’s multiple comparison test) to assess statistical significance. ∗∗∗∗ denotes a p -value of <0.0001. All the AFM samples were prepared on freshly cleaved mica surfaces, and imaging was performed in intermittent tapping mode using Bruker NanoWizard 3 (JPK instruments); data was processed using the JPK data processing tool (scale bar- 1 μm). C , cross-seeding assay by sonicated α-Synuclein pre-formed fibrils shows they can nucleate wild-type ( violet ) but not the mutants F133A (black) and V173K ( orange ) as observed from the light scattering assay.
    Figure Legend Snippet: Heterotypic aggregation: in-vitro coaggregation of OTUB1 and α-Synuclein. A and B , seeding effects of OTUB1 aggregates on the aggregation of α-Synuclein were studied by using ThT binding kinetics ( A ) and atomic force microscopy ( B ). OTUB1 seeds (5 μM) were added at 0 h to 300 μM of α-Synuclein and aggregation kinetics were performed using ThT dye binding assay. Aliquotes at different time intervals were collected to measure ThT fluorescence and for AFM sample preparation. The ThT data was plotted in GraphPad Prism and fitted using a non-linear fit sigmoidal model, and subjected to a two-way ANOVA (Dunnett’s multiple comparison test) to assess statistical significance. ∗∗∗∗ denotes a p -value of <0.0001. All the AFM samples were prepared on freshly cleaved mica surfaces, and imaging was performed in intermittent tapping mode using Bruker NanoWizard 3 (JPK instruments); data was processed using the JPK data processing tool (scale bar- 1 μm). C , cross-seeding assay by sonicated α-Synuclein pre-formed fibrils shows they can nucleate wild-type ( violet ) but not the mutants F133A (black) and V173K ( orange ) as observed from the light scattering assay.

    Techniques Used: In Vitro, Binding Assay, Microscopy, Fluorescence, Sample Prep, Comparison, Imaging, Cross Seeding Assay, Sonication, Scattering Assay

    Related Articles

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    Article Title: Hotspot site microenvironment in the deubiquitinase OTUB1 drives its stability and aggregation
    Article Snippet: .. All the AFM samples were prepared on freshly cleaved mica surfaces, and imaging was performed in intermittent tapping mode using Bruker NanoWizard 3 (JPK instruments); data was processed using the JPK data processing tool (scale bar- 1 μm). ..



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