Saline:Article Title: A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation
Article Snippet: .. L-Azidohomoalanine (AHA; Iris Biotech GmbH), Cycloheximide (CHX; ACROS Organics), Tris (2-carboxyethy)phosphine hydrochloride (TCEP; Sigma Aldrich), 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne; Jena Bioscience), Tris [(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA; Sigma Aldrich), Dimethyl sulfoxide (DMSO; Sigma Aldrich), Copper sulphate (CuSO 4 ; Sigma Aldrich), 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris; Sigma Aldrich), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES; Sigma Aldrich), Sodium chloride (NaCl; Fisher Scientific), Sodium dodecyl sulphate (SDS; Fisher Scientific), Sodium bicarbonate (NaHCO 3 ; ACROS Organics), Calcium chloride (CaCl 2 ; Sigma Aldrich), Urea (Sigma Aldrich), 1,4-Dithiothreitol (DTT; Sigma Aldrich), 2-Chloroacetamide (CAA; Sigma Aldrich), L-Glutamine solution (Sigma Aldrich), Benzonase (Sigma Aldrich), DCTM Protein Assay (Bio-Rad), Dialysed Foetal Bovine Serum (FBS; Life Technologies), Schneider’s Insect Medium (Sigma-Aldrich), Schneider’s Drosophila Medium without L-Methionine (PAN Biotech), Dulbecco Phosphate Buffered Saline (DPBS, Gibco), 1M Triethylammonium bicarbonate (TEAB) buffer (Sigma Aldrich), NeutrAvidin-Agarose beads (Thermo Scientific), iTRAQ® Reagents Multiplex Kit (Sigma Aldrich), OptimaTM LC-MS Grade Trifluoroacetic acid (TFA; Fisher Scientific), OptimaTM LC-MS Grade Formic acid (TFA; Fisher Scientific), OptimaTM LC-MS Grade Acetonitrile (CAN; Fisher Scientific), OptimaTM LC-MS Grade Methanol (MeOH; Fisher Scientific) and Sequencing Grade Modified Trypsin (Promega). .. Promastigote form of L. mexicana strain M379 (MNYC/BC/62/M379) were grown in T-25 flasks at 26 °C in Schneider’s Insect Medium (Sigma-Aldrich) supplemented with 0.4g/L NaHCO 3 , 0.6g/L anhydrous CaCl 2 and 10% FBS (pH 7.2).
Article Title: A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation
Article Snippet: .. L-Azidohomoalanine (AHA; Iris Biotech GmbH), Cycloheximide (CHX; ACROS Organics), Tris (2-carboxyethy)phosphine hydrochloride (TCEP; Sigma Aldrich), 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne; Jena Bioscience), Tris [(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA; Sigma Aldrich), Dimethyl sulfoxide (DMSO; Sigma Aldrich), Copper sulphate (CuSO 4 ; Sigma Aldrich), 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris; Sigma Aldrich), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES; Sigma Aldrich), Sodium chloride (NaCl; Fisher Scientific), Sodium dodecyl sulphate (SDS; Fisher Scientific), Sodium bicarbonate (NaHCO 3 ; ACROS Organics), Calcium chloride (CaCl 2 ; Sigma Aldrich), Urea (Sigma Aldrich), 1,4-Dithiothreitol (DTT; Sigma Aldrich), 2-Chloroacetamide (CAA; Sigma Aldrich), L-Glutamine solution (Sigma Aldrich), Benzonase (Sigma Aldrich), DC Protein Assay (Bio-Rad), Dialysed Foetal Bovine Serum (FBS; Life Technologies), Schneider’s Insect Medium (Sigma-Aldrich), Schneider’s Drosophila Medium without L-Methionine (PAN Biotech), Dulbecco Phosphate Buffered Saline (DPBS, Gibco), 1M Triethylammonium bicarbonate (TEAB) buffer (Sigma Aldrich), NeutrAvidin-Agarose beads (Thermo Scientific), iTRAQ Reagents Multiplex Kit (Sigma Aldrich), Optima LC-MS Grade Trifluoroacetic acid (TFA; Fisher Scientific), Optima LC-MS Grade Formic acid (FA; Fisher Scientific), Optima LC-MS Grade Acetonitrile (ACN; Fisher Scientific), Optima LC-MS Grade Methanol (MeOH; Fisher Scientific) and Sequencing Grade Modified Trypsin (Promega). .. Promastigote form of L . mexicana strain M379 (MNYC/BC/62/M379) were grown in T-25 flasks at 26 o C in Schneider’s Insect Medium (Sigma-Aldrich) supplemented with 0.4g/L NaHCO 3 , 0.6g/L anhydrous CaCl 2 and 10% FBS (pH 7.2).
Multiplex sample analysis:Article Title: A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation
Article Snippet: .. L-Azidohomoalanine (AHA; Iris Biotech GmbH), Cycloheximide (CHX; ACROS Organics), Tris (2-carboxyethy)phosphine hydrochloride (TCEP; Sigma Aldrich), 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne; Jena Bioscience), Tris [(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA; Sigma Aldrich), Dimethyl sulfoxide (DMSO; Sigma Aldrich), Copper sulphate (CuSO 4 ; Sigma Aldrich), 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris; Sigma Aldrich), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES; Sigma Aldrich), Sodium chloride (NaCl; Fisher Scientific), Sodium dodecyl sulphate (SDS; Fisher Scientific), Sodium bicarbonate (NaHCO 3 ; ACROS Organics), Calcium chloride (CaCl 2 ; Sigma Aldrich), Urea (Sigma Aldrich), 1,4-Dithiothreitol (DTT; Sigma Aldrich), 2-Chloroacetamide (CAA; Sigma Aldrich), L-Glutamine solution (Sigma Aldrich), Benzonase (Sigma Aldrich), DCTM Protein Assay (Bio-Rad), Dialysed Foetal Bovine Serum (FBS; Life Technologies), Schneider’s Insect Medium (Sigma-Aldrich), Schneider’s Drosophila Medium without L-Methionine (PAN Biotech), Dulbecco Phosphate Buffered Saline (DPBS, Gibco), 1M Triethylammonium bicarbonate (TEAB) buffer (Sigma Aldrich), NeutrAvidin-Agarose beads (Thermo Scientific), iTRAQ® Reagents Multiplex Kit (Sigma Aldrich), OptimaTM LC-MS Grade Trifluoroacetic acid (TFA; Fisher Scientific), OptimaTM LC-MS Grade Formic acid (TFA; Fisher Scientific), OptimaTM LC-MS Grade Acetonitrile (CAN; Fisher Scientific), OptimaTM LC-MS Grade Methanol (MeOH; Fisher Scientific) and Sequencing Grade Modified Trypsin (Promega). .. Promastigote form of L. mexicana strain M379 (MNYC/BC/62/M379) were grown in T-25 flasks at 26 °C in Schneider’s Insect Medium (Sigma-Aldrich) supplemented with 0.4g/L NaHCO 3 , 0.6g/L anhydrous CaCl 2 and 10% FBS (pH 7.2).
Article Title: A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation
Article Snippet: .. L-Azidohomoalanine (AHA; Iris Biotech GmbH), Cycloheximide (CHX; ACROS Organics), Tris (2-carboxyethy)phosphine hydrochloride (TCEP; Sigma Aldrich), 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne; Jena Bioscience), Tris [(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA; Sigma Aldrich), Dimethyl sulfoxide (DMSO; Sigma Aldrich), Copper sulphate (CuSO 4 ; Sigma Aldrich), 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris; Sigma Aldrich), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES; Sigma Aldrich), Sodium chloride (NaCl; Fisher Scientific), Sodium dodecyl sulphate (SDS; Fisher Scientific), Sodium bicarbonate (NaHCO 3 ; ACROS Organics), Calcium chloride (CaCl 2 ; Sigma Aldrich), Urea (Sigma Aldrich), 1,4-Dithiothreitol (DTT; Sigma Aldrich), 2-Chloroacetamide (CAA; Sigma Aldrich), L-Glutamine solution (Sigma Aldrich), Benzonase (Sigma Aldrich), DC Protein Assay (Bio-Rad), Dialysed Foetal Bovine Serum (FBS; Life Technologies), Schneider’s Insect Medium (Sigma-Aldrich), Schneider’s Drosophila Medium without L-Methionine (PAN Biotech), Dulbecco Phosphate Buffered Saline (DPBS, Gibco), 1M Triethylammonium bicarbonate (TEAB) buffer (Sigma Aldrich), NeutrAvidin-Agarose beads (Thermo Scientific), iTRAQ Reagents Multiplex Kit (Sigma Aldrich), Optima LC-MS Grade Trifluoroacetic acid (TFA; Fisher Scientific), Optima LC-MS Grade Formic acid (FA; Fisher Scientific), Optima LC-MS Grade Acetonitrile (ACN; Fisher Scientific), Optima LC-MS Grade Methanol (MeOH; Fisher Scientific) and Sequencing Grade Modified Trypsin (Promega). .. Promastigote form of L . mexicana strain M379 (MNYC/BC/62/M379) were grown in T-25 flasks at 26 o C in Schneider’s Insect Medium (Sigma-Aldrich) supplemented with 0.4g/L NaHCO 3 , 0.6g/L anhydrous CaCl 2 and 10% FBS (pH 7.2).
Multiplex Assay:Article Title: A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation
Article Snippet: .. L-Azidohomoalanine (AHA; Iris Biotech GmbH), Cycloheximide (CHX; ACROS Organics), Tris (2-carboxyethy)phosphine hydrochloride (TCEP; Sigma Aldrich), 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne; Jena Bioscience), Tris [(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA; Sigma Aldrich), Dimethyl sulfoxide (DMSO; Sigma Aldrich), Copper sulphate (CuSO 4 ; Sigma Aldrich), 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris; Sigma Aldrich), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES; Sigma Aldrich), Sodium chloride (NaCl; Fisher Scientific), Sodium dodecyl sulphate (SDS; Fisher Scientific), Sodium bicarbonate (NaHCO 3 ; ACROS Organics), Calcium chloride (CaCl 2 ; Sigma Aldrich), Urea (Sigma Aldrich), 1,4-Dithiothreitol (DTT; Sigma Aldrich), 2-Chloroacetamide (CAA; Sigma Aldrich), L-Glutamine solution (Sigma Aldrich), Benzonase (Sigma Aldrich), DCTM Protein Assay (Bio-Rad), Dialysed Foetal Bovine Serum (FBS; Life Technologies), Schneider’s Insect Medium (Sigma-Aldrich), Schneider’s Drosophila Medium without L-Methionine (PAN Biotech), Dulbecco Phosphate Buffered Saline (DPBS, Gibco), 1M Triethylammonium bicarbonate (TEAB) buffer (Sigma Aldrich), NeutrAvidin-Agarose beads (Thermo Scientific), iTRAQ® Reagents Multiplex Kit (Sigma Aldrich), OptimaTM LC-MS Grade Trifluoroacetic acid (TFA; Fisher Scientific), OptimaTM LC-MS Grade Formic acid (TFA; Fisher Scientific), OptimaTM LC-MS Grade Acetonitrile (CAN; Fisher Scientific), OptimaTM LC-MS Grade Methanol (MeOH; Fisher Scientific) and Sequencing Grade Modified Trypsin (Promega). .. Promastigote form of L. mexicana strain M379 (MNYC/BC/62/M379) were grown in T-25 flasks at 26 °C in Schneider’s Insect Medium (Sigma-Aldrich) supplemented with 0.4g/L NaHCO 3 , 0.6g/L anhydrous CaCl 2 and 10% FBS (pH 7.2).
Article Title: A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation
Article Snippet: .. L-Azidohomoalanine (AHA; Iris Biotech GmbH), Cycloheximide (CHX; ACROS Organics), Tris (2-carboxyethy)phosphine hydrochloride (TCEP; Sigma Aldrich), 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne; Jena Bioscience), Tris [(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA; Sigma Aldrich), Dimethyl sulfoxide (DMSO; Sigma Aldrich), Copper sulphate (CuSO 4 ; Sigma Aldrich), 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris; Sigma Aldrich), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES; Sigma Aldrich), Sodium chloride (NaCl; Fisher Scientific), Sodium dodecyl sulphate (SDS; Fisher Scientific), Sodium bicarbonate (NaHCO 3 ; ACROS Organics), Calcium chloride (CaCl 2 ; Sigma Aldrich), Urea (Sigma Aldrich), 1,4-Dithiothreitol (DTT; Sigma Aldrich), 2-Chloroacetamide (CAA; Sigma Aldrich), L-Glutamine solution (Sigma Aldrich), Benzonase (Sigma Aldrich), DC Protein Assay (Bio-Rad), Dialysed Foetal Bovine Serum (FBS; Life Technologies), Schneider’s Insect Medium (Sigma-Aldrich), Schneider’s Drosophila Medium without L-Methionine (PAN Biotech), Dulbecco Phosphate Buffered Saline (DPBS, Gibco), 1M Triethylammonium bicarbonate (TEAB) buffer (Sigma Aldrich), NeutrAvidin-Agarose beads (Thermo Scientific), iTRAQ Reagents Multiplex Kit (Sigma Aldrich), Optima LC-MS Grade Trifluoroacetic acid (TFA; Fisher Scientific), Optima LC-MS Grade Formic acid (FA; Fisher Scientific), Optima LC-MS Grade Acetonitrile (ACN; Fisher Scientific), Optima LC-MS Grade Methanol (MeOH; Fisher Scientific) and Sequencing Grade Modified Trypsin (Promega). .. Promastigote form of L . mexicana strain M379 (MNYC/BC/62/M379) were grown in T-25 flasks at 26 o C in Schneider’s Insect Medium (Sigma-Aldrich) supplemented with 0.4g/L NaHCO 3 , 0.6g/L anhydrous CaCl 2 and 10% FBS (pH 7.2).
Liquid Chromatography with Mass Spectroscopy:Article Title: A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation
Article Snippet: .. L-Azidohomoalanine (AHA; Iris Biotech GmbH), Cycloheximide (CHX; ACROS Organics), Tris (2-carboxyethy)phosphine hydrochloride (TCEP; Sigma Aldrich), 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne; Jena Bioscience), Tris [(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA; Sigma Aldrich), Dimethyl sulfoxide (DMSO; Sigma Aldrich), Copper sulphate (CuSO 4 ; Sigma Aldrich), 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris; Sigma Aldrich), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES; Sigma Aldrich), Sodium chloride (NaCl; Fisher Scientific), Sodium dodecyl sulphate (SDS; Fisher Scientific), Sodium bicarbonate (NaHCO 3 ; ACROS Organics), Calcium chloride (CaCl 2 ; Sigma Aldrich), Urea (Sigma Aldrich), 1,4-Dithiothreitol (DTT; Sigma Aldrich), 2-Chloroacetamide (CAA; Sigma Aldrich), L-Glutamine solution (Sigma Aldrich), Benzonase (Sigma Aldrich), DCTM Protein Assay (Bio-Rad), Dialysed Foetal Bovine Serum (FBS; Life Technologies), Schneider’s Insect Medium (Sigma-Aldrich), Schneider’s Drosophila Medium without L-Methionine (PAN Biotech), Dulbecco Phosphate Buffered Saline (DPBS, Gibco), 1M Triethylammonium bicarbonate (TEAB) buffer (Sigma Aldrich), NeutrAvidin-Agarose beads (Thermo Scientific), iTRAQ® Reagents Multiplex Kit (Sigma Aldrich), OptimaTM LC-MS Grade Trifluoroacetic acid (TFA; Fisher Scientific), OptimaTM LC-MS Grade Formic acid (TFA; Fisher Scientific), OptimaTM LC-MS Grade Acetonitrile (CAN; Fisher Scientific), OptimaTM LC-MS Grade Methanol (MeOH; Fisher Scientific) and Sequencing Grade Modified Trypsin (Promega). .. Promastigote form of L. mexicana strain M379 (MNYC/BC/62/M379) were grown in T-25 flasks at 26 °C in Schneider’s Insect Medium (Sigma-Aldrich) supplemented with 0.4g/L NaHCO 3 , 0.6g/L anhydrous CaCl 2 and 10% FBS (pH 7.2).
Article Title: A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation
Article Snippet: .. L-Azidohomoalanine (AHA; Iris Biotech GmbH), Cycloheximide (CHX; ACROS Organics), Tris (2-carboxyethy)phosphine hydrochloride (TCEP; Sigma Aldrich), 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne; Jena Bioscience), Tris [(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA; Sigma Aldrich), Dimethyl sulfoxide (DMSO; Sigma Aldrich), Copper sulphate (CuSO 4 ; Sigma Aldrich), 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris; Sigma Aldrich), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES; Sigma Aldrich), Sodium chloride (NaCl; Fisher Scientific), Sodium dodecyl sulphate (SDS; Fisher Scientific), Sodium bicarbonate (NaHCO 3 ; ACROS Organics), Calcium chloride (CaCl 2 ; Sigma Aldrich), Urea (Sigma Aldrich), 1,4-Dithiothreitol (DTT; Sigma Aldrich), 2-Chloroacetamide (CAA; Sigma Aldrich), L-Glutamine solution (Sigma Aldrich), Benzonase (Sigma Aldrich), DC Protein Assay (Bio-Rad), Dialysed Foetal Bovine Serum (FBS; Life Technologies), Schneider’s Insect Medium (Sigma-Aldrich), Schneider’s Drosophila Medium without L-Methionine (PAN Biotech), Dulbecco Phosphate Buffered Saline (DPBS, Gibco), 1M Triethylammonium bicarbonate (TEAB) buffer (Sigma Aldrich), NeutrAvidin-Agarose beads (Thermo Scientific), iTRAQ Reagents Multiplex Kit (Sigma Aldrich), Optima LC-MS Grade Trifluoroacetic acid (TFA; Fisher Scientific), Optima LC-MS Grade Formic acid (FA; Fisher Scientific), Optima LC-MS Grade Acetonitrile (ACN; Fisher Scientific), Optima LC-MS Grade Methanol (MeOH; Fisher Scientific) and Sequencing Grade Modified Trypsin (Promega). .. Promastigote form of L . mexicana strain M379 (MNYC/BC/62/M379) were grown in T-25 flasks at 26 o C in Schneider’s Insect Medium (Sigma-Aldrich) supplemented with 0.4g/L NaHCO 3 , 0.6g/L anhydrous CaCl 2 and 10% FBS (pH 7.2).
Sequencing:Article Title: A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation
Article Snippet: .. L-Azidohomoalanine (AHA; Iris Biotech GmbH), Cycloheximide (CHX; ACROS Organics), Tris (2-carboxyethy)phosphine hydrochloride (TCEP; Sigma Aldrich), 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne; Jena Bioscience), Tris [(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA; Sigma Aldrich), Dimethyl sulfoxide (DMSO; Sigma Aldrich), Copper sulphate (CuSO 4 ; Sigma Aldrich), 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris; Sigma Aldrich), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES; Sigma Aldrich), Sodium chloride (NaCl; Fisher Scientific), Sodium dodecyl sulphate (SDS; Fisher Scientific), Sodium bicarbonate (NaHCO 3 ; ACROS Organics), Calcium chloride (CaCl 2 ; Sigma Aldrich), Urea (Sigma Aldrich), 1,4-Dithiothreitol (DTT; Sigma Aldrich), 2-Chloroacetamide (CAA; Sigma Aldrich), L-Glutamine solution (Sigma Aldrich), Benzonase (Sigma Aldrich), DCTM Protein Assay (Bio-Rad), Dialysed Foetal Bovine Serum (FBS; Life Technologies), Schneider’s Insect Medium (Sigma-Aldrich), Schneider’s Drosophila Medium without L-Methionine (PAN Biotech), Dulbecco Phosphate Buffered Saline (DPBS, Gibco), 1M Triethylammonium bicarbonate (TEAB) buffer (Sigma Aldrich), NeutrAvidin-Agarose beads (Thermo Scientific), iTRAQ® Reagents Multiplex Kit (Sigma Aldrich), OptimaTM LC-MS Grade Trifluoroacetic acid (TFA; Fisher Scientific), OptimaTM LC-MS Grade Formic acid (TFA; Fisher Scientific), OptimaTM LC-MS Grade Acetonitrile (CAN; Fisher Scientific), OptimaTM LC-MS Grade Methanol (MeOH; Fisher Scientific) and Sequencing Grade Modified Trypsin (Promega). .. Promastigote form of L. mexicana strain M379 (MNYC/BC/62/M379) were grown in T-25 flasks at 26 °C in Schneider’s Insect Medium (Sigma-Aldrich) supplemented with 0.4g/L NaHCO 3 , 0.6g/L anhydrous CaCl 2 and 10% FBS (pH 7.2).
Article Title: A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation
Article Snippet: .. L-Azidohomoalanine (AHA; Iris Biotech GmbH), Cycloheximide (CHX; ACROS Organics), Tris (2-carboxyethy)phosphine hydrochloride (TCEP; Sigma Aldrich), 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne; Jena Bioscience), Tris [(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA; Sigma Aldrich), Dimethyl sulfoxide (DMSO; Sigma Aldrich), Copper sulphate (CuSO 4 ; Sigma Aldrich), 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris; Sigma Aldrich), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES; Sigma Aldrich), Sodium chloride (NaCl; Fisher Scientific), Sodium dodecyl sulphate (SDS; Fisher Scientific), Sodium bicarbonate (NaHCO 3 ; ACROS Organics), Calcium chloride (CaCl 2 ; Sigma Aldrich), Urea (Sigma Aldrich), 1,4-Dithiothreitol (DTT; Sigma Aldrich), 2-Chloroacetamide (CAA; Sigma Aldrich), L-Glutamine solution (Sigma Aldrich), Benzonase (Sigma Aldrich), DC Protein Assay (Bio-Rad), Dialysed Foetal Bovine Serum (FBS; Life Technologies), Schneider’s Insect Medium (Sigma-Aldrich), Schneider’s Drosophila Medium without L-Methionine (PAN Biotech), Dulbecco Phosphate Buffered Saline (DPBS, Gibco), 1M Triethylammonium bicarbonate (TEAB) buffer (Sigma Aldrich), NeutrAvidin-Agarose beads (Thermo Scientific), iTRAQ Reagents Multiplex Kit (Sigma Aldrich), Optima LC-MS Grade Trifluoroacetic acid (TFA; Fisher Scientific), Optima LC-MS Grade Formic acid (FA; Fisher Scientific), Optima LC-MS Grade Acetonitrile (ACN; Fisher Scientific), Optima LC-MS Grade Methanol (MeOH; Fisher Scientific) and Sequencing Grade Modified Trypsin (Promega). .. Promastigote form of L . mexicana strain M379 (MNYC/BC/62/M379) were grown in T-25 flasks at 26 o C in Schneider’s Insect Medium (Sigma-Aldrich) supplemented with 0.4g/L NaHCO 3 , 0.6g/L anhydrous CaCl 2 and 10% FBS (pH 7.2).
Modification:Article Title: A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation
Article Snippet: .. L-Azidohomoalanine (AHA; Iris Biotech GmbH), Cycloheximide (CHX; ACROS Organics), Tris (2-carboxyethy)phosphine hydrochloride (TCEP; Sigma Aldrich), 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne; Jena Bioscience), Tris [(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA; Sigma Aldrich), Dimethyl sulfoxide (DMSO; Sigma Aldrich), Copper sulphate (CuSO 4 ; Sigma Aldrich), 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris; Sigma Aldrich), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES; Sigma Aldrich), Sodium chloride (NaCl; Fisher Scientific), Sodium dodecyl sulphate (SDS; Fisher Scientific), Sodium bicarbonate (NaHCO 3 ; ACROS Organics), Calcium chloride (CaCl 2 ; Sigma Aldrich), Urea (Sigma Aldrich), 1,4-Dithiothreitol (DTT; Sigma Aldrich), 2-Chloroacetamide (CAA; Sigma Aldrich), L-Glutamine solution (Sigma Aldrich), Benzonase (Sigma Aldrich), DCTM Protein Assay (Bio-Rad), Dialysed Foetal Bovine Serum (FBS; Life Technologies), Schneider’s Insect Medium (Sigma-Aldrich), Schneider’s Drosophila Medium without L-Methionine (PAN Biotech), Dulbecco Phosphate Buffered Saline (DPBS, Gibco), 1M Triethylammonium bicarbonate (TEAB) buffer (Sigma Aldrich), NeutrAvidin-Agarose beads (Thermo Scientific), iTRAQ® Reagents Multiplex Kit (Sigma Aldrich), OptimaTM LC-MS Grade Trifluoroacetic acid (TFA; Fisher Scientific), OptimaTM LC-MS Grade Formic acid (TFA; Fisher Scientific), OptimaTM LC-MS Grade Acetonitrile (CAN; Fisher Scientific), OptimaTM LC-MS Grade Methanol (MeOH; Fisher Scientific) and Sequencing Grade Modified Trypsin (Promega). .. Promastigote form of L. mexicana strain M379 (MNYC/BC/62/M379) were grown in T-25 flasks at 26 °C in Schneider’s Insect Medium (Sigma-Aldrich) supplemented with 0.4g/L NaHCO 3 , 0.6g/L anhydrous CaCl 2 and 10% FBS (pH 7.2).
Article Title: A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation
Article Snippet: .. L-Azidohomoalanine (AHA; Iris Biotech GmbH), Cycloheximide (CHX; ACROS Organics), Tris (2-carboxyethy)phosphine hydrochloride (TCEP; Sigma Aldrich), 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne; Jena Bioscience), Tris [(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA; Sigma Aldrich), Dimethyl sulfoxide (DMSO; Sigma Aldrich), Copper sulphate (CuSO 4 ; Sigma Aldrich), 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris; Sigma Aldrich), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES; Sigma Aldrich), Sodium chloride (NaCl; Fisher Scientific), Sodium dodecyl sulphate (SDS; Fisher Scientific), Sodium bicarbonate (NaHCO 3 ; ACROS Organics), Calcium chloride (CaCl 2 ; Sigma Aldrich), Urea (Sigma Aldrich), 1,4-Dithiothreitol (DTT; Sigma Aldrich), 2-Chloroacetamide (CAA; Sigma Aldrich), L-Glutamine solution (Sigma Aldrich), Benzonase (Sigma Aldrich), DC Protein Assay (Bio-Rad), Dialysed Foetal Bovine Serum (FBS; Life Technologies), Schneider’s Insect Medium (Sigma-Aldrich), Schneider’s Drosophila Medium without L-Methionine (PAN Biotech), Dulbecco Phosphate Buffered Saline (DPBS, Gibco), 1M Triethylammonium bicarbonate (TEAB) buffer (Sigma Aldrich), NeutrAvidin-Agarose beads (Thermo Scientific), iTRAQ Reagents Multiplex Kit (Sigma Aldrich), Optima LC-MS Grade Trifluoroacetic acid (TFA; Fisher Scientific), Optima LC-MS Grade Formic acid (FA; Fisher Scientific), Optima LC-MS Grade Acetonitrile (ACN; Fisher Scientific), Optima LC-MS Grade Methanol (MeOH; Fisher Scientific) and Sequencing Grade Modified Trypsin (Promega). .. Promastigote form of L . mexicana strain M379 (MNYC/BC/62/M379) were grown in T-25 flasks at 26 o C in Schneider’s Insect Medium (Sigma-Aldrich) supplemented with 0.4g/L NaHCO 3 , 0.6g/L anhydrous CaCl 2 and 10% FBS (pH 7.2).
DC Protein Assay:Article Title: A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation
Article Snippet: .. L-Azidohomoalanine (AHA; Iris Biotech GmbH), Cycloheximide (CHX; ACROS Organics), Tris (2-carboxyethy)phosphine hydrochloride (TCEP; Sigma Aldrich), 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne; Jena Bioscience), Tris [(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA; Sigma Aldrich), Dimethyl sulfoxide (DMSO; Sigma Aldrich), Copper sulphate (CuSO 4 ; Sigma Aldrich), 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris; Sigma Aldrich), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES; Sigma Aldrich), Sodium chloride (NaCl; Fisher Scientific), Sodium dodecyl sulphate (SDS; Fisher Scientific), Sodium bicarbonate (NaHCO 3 ; ACROS Organics), Calcium chloride (CaCl 2 ; Sigma Aldrich), Urea (Sigma Aldrich), 1,4-Dithiothreitol (DTT; Sigma Aldrich), 2-Chloroacetamide (CAA; Sigma Aldrich), L-Glutamine solution (Sigma Aldrich), Benzonase (Sigma Aldrich), DC Protein Assay (Bio-Rad), Dialysed Foetal Bovine Serum (FBS; Life Technologies), Schneider’s Insect Medium (Sigma-Aldrich), Schneider’s Drosophila Medium without L-Methionine (PAN Biotech), Dulbecco Phosphate Buffered Saline (DPBS, Gibco), 1M Triethylammonium bicarbonate (TEAB) buffer (Sigma Aldrich), NeutrAvidin-Agarose beads (Thermo Scientific), iTRAQ Reagents Multiplex Kit (Sigma Aldrich), Optima LC-MS Grade Trifluoroacetic acid (TFA; Fisher Scientific), Optima LC-MS Grade Formic acid (FA; Fisher Scientific), Optima LC-MS Grade Acetonitrile (ACN; Fisher Scientific), Optima LC-MS Grade Methanol (MeOH; Fisher Scientific) and Sequencing Grade Modified Trypsin (Promega). .. Promastigote form of L . mexicana strain M379 (MNYC/BC/62/M379) were grown in T-25 flasks at 26 o C in Schneider’s Insect Medium (Sigma-Aldrich) supplemented with 0.4g/L NaHCO 3 , 0.6g/L anhydrous CaCl 2 and 10% FBS (pH 7.2).
Polymer:Article Title: Bioinspired Confinement of Upconversion Nanoparticles for Improved Performance in Aqueous Solution
Article Snippet: The resonance energy transfer (RET) from NaYF4:Yb,Er upconverting nanoparticles (UNCPs) to a dye (5-carboxytetramethylrhodamine (TAMRA)) was investigated by photoluminescence experiments and microscale thermophoresis (MST).. The dye was excited via RET from the UCNPs which was excited in the near-infrared (NIR).. The change of the dye diffusion speed (free vs coupled) was investigated by MST.
Purification:Article Title: A Semi-Rationally Engineered Bacterial Pyrrolysyl-tRNA Synthetase Genetically Encodes Phenyl Azide Chemistry.
Article Snippet: A semi-rationally engineered bacterial pyrrolysyl-tRNA synthetase genetically encodes phenyl azide chemistry† Patrik Fladischer1,2 Alexandra Weingartner1,2,† Johannes Blamauer1,2 Barbara Darnhofer1,3,4 Ruth Birner-Gruenberger3,4 Tsvetan Kardashliev5,‡ Anna Joelle Ruff5 Ulrich Schwaneberg5 Birgit Wiltschi1 1acib Austrian Centre of Industrial Biotechnology, Graz, Austria 2Institute of Molecular Biotechnology, Graz University of Technology, Graz, Austria 3Research Unit Functional Proteomics and Metabolomic Pathways, Institute of Pathology, Medical University of Graz, Graz, Austria 4Omics Center Graz, BioTechMed-Graz, Graz, Austria 5Lehrstuhl für Biotechnologie, RWTH Aachen University, Aachen, Germany †present address: Lehrstuhl für Biotechnologie, RWTH Aachen University, Aachen, Germany ‡present address: D-BSSE Bioprocess Laboratory, ETH Zürich, Basel, Switzerland Correspondence: Dr. Birgit Wiltschi, acib Austrian Centre of Industrial Biotechnology, Petersgasse 14, A-8010 Graz, Austria E-mail: birgit.wiltschi@acib.at
Incubation:Article Title: A Semi-Rationally Engineered Bacterial Pyrrolysyl-tRNA Synthetase Genetically Encodes Phenyl Azide Chemistry.
Article Snippet: A semi-rationally engineered bacterial pyrrolysyl-tRNA synthetase genetically encodes phenyl azide chemistry† Patrik Fladischer1,2 Alexandra Weingartner1,2,† Johannes Blamauer1,2 Barbara Darnhofer1,3,4 Ruth Birner-Gruenberger3,4 Tsvetan Kardashliev5,‡ Anna Joelle Ruff5 Ulrich Schwaneberg5 Birgit Wiltschi1 1acib Austrian Centre of Industrial Biotechnology, Graz, Austria 2Institute of Molecular Biotechnology, Graz University of Technology, Graz, Austria 3Research Unit Functional Proteomics and Metabolomic Pathways, Institute of Pathology, Medical University of Graz, Graz, Austria 4Omics Center Graz, BioTechMed-Graz, Graz, Austria 5Lehrstuhl für Biotechnologie, RWTH Aachen University, Aachen, Germany †present address: Lehrstuhl für Biotechnologie, RWTH Aachen University, Aachen, Germany ‡present address: D-BSSE Bioprocess Laboratory, ETH Zürich, Basel, Switzerland Correspondence: Dr. Birgit Wiltschi, acib Austrian Centre of Industrial Biotechnology, Petersgasse 14, A-8010 Graz, Austria E-mail: birgit.wiltschi@acib.at
|
Jena Bioscience is a verified supplier 
Jena Bioscience manufactures this product 