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3d tissue clearing method (Vienna Biocenter Core Facilities GmbH)

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Vienna Biocenter Core Facilities GmbH 3d tissue clearing method

a ECi-based <t>iCO</t> <t>clearing</t> and HCS imaging workflow. b Transparency of iCOs after the ECi tissue clearing. c , d Representative <t>3D</t> organoid imaging using HCS system and 3D imaging technique after ECi-based tissue clearing procedure. Aβ (red) and p-tau (green) antibodies were used (scale bar, 1 mm) ( p -value criteria: # p < 0.1, * p < 0.05, ** p < 0.01, and *** p < 0.001; for PiB − iCOs and PiB + iCOs, *** p = 0.0007 for Aβ, * p = 0.0474 for p-tau; for E4 iso iCOs and E3 par iCOs, * p = 0.0135 for Aβ, # p = 0.0841 for p-tau; two-sided p -values; unpaired t -test; 10-20 iCOs were used for each group). White arrows, position of Aβ aggregates in the iCOs; White scale bar, 0.5 mm. PiB Pittsburgh compound B, Aβ beta-amyloid, p-tau phosphorylated tau, IntDen integrated density.

3d Tissue Clearing Method, supplied by Vienna Biocenter Core Facilities GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clearing+method/3d+tissue+clearing+method/pmc07804132-101-2-15
Average 90 stars, based on 1 article reviews

3d tissue clearing method - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "A logical network-based drug-screening platform for Alzheimer’s disease representing pathological features of human brain organoids"

Article Title: A logical network-based drug-screening platform for Alzheimer’s disease representing pathological features of human brain organoids

Journal: Nature Communications

doi: 10.1038/s41467-020-20440-5

a ECi-based iCO clearing and HCS imaging workflow. b Transparency of iCOs after the ECi tissue clearing. c , d Representative 3D organoid imaging using HCS system and 3D imaging technique after ECi-based tissue clearing procedure. Aβ (red) and p-tau (green) antibodies were used (scale bar, 1 mm) ( p -value criteria: # p < 0.1, * p < 0.05, ** p < 0.01, and *** p < 0.001; for PiB − iCOs and PiB + iCOs, *** p = 0.0007 for Aβ, * p = 0.0474 for p-tau; for E4 iso iCOs and E3 par iCOs, * p = 0.0135 for Aβ, # p = 0.0841 for p-tau; two-sided p -values; unpaired t -test; 10-20 iCOs were used for each group). White arrows, position of Aβ aggregates in the iCOs; White scale bar, 0.5 mm. PiB Pittsburgh compound B, Aβ beta-amyloid, p-tau phosphorylated tau, IntDen integrated density.

Figure Legend Snippet: a ECi-based iCO clearing and HCS imaging workflow. b Transparency of iCOs after the ECi tissue clearing. c , d Representative 3D organoid imaging using HCS system and 3D imaging technique after ECi-based tissue clearing procedure. Aβ (red) and p-tau (green) antibodies were used (scale bar, 1 mm) ( p -value criteria: # p < 0.1, * p < 0.05, ** p < 0.01, and *** p < 0.001; for PiB − iCOs and PiB + iCOs, *** p = 0.0007 for Aβ, * p = 0.0474 for p-tau; for E4 iso iCOs and E3 par iCOs, * p = 0.0135 for Aβ, # p = 0.0841 for p-tau; two-sided p -values; unpaired t -test; 10-20 iCOs were used for each group). White arrows, position of Aβ aggregates in the iCOs; White scale bar, 0.5 mm. PiB Pittsburgh compound B, Aβ beta-amyloid, p-tau phosphorylated tau, IntDen integrated density.

Techniques Used: Imaging

Imaging:

Article Title: A logical network-based drug-screening platform for Alzheimer’s disease representing pathological features of human brain organoids
Article Snippet: the conditioned media in Fig. , we need to examine whether iCOs could exhibit pathological lesions reminiscent of those of real human brain tissues. .. We used 3D tissue clearing method to create a uniform index following a protocol from Vienna Biocenter (VBC) using ethyl cinnamate (ECi) as an index-matching solution (Fig. ). .. After tissue clearing, iCOs exhibited transparency and were invisible to the naked eye (Fig. ).

Image Search Results

Journal: STAR Protocols

Article Title: Determining glioma cell invasion and proliferation in ex vivo organotypic mouse brain slices using whole-mount immunostaining and tissue clearing

doi: 10.1016/j.xpro.2022.101703

Figure Lengend Snippet:

Article Snippet: The described protocol is an adaptation of the whole-mount immunostaining protocol developed by ( ) and the RapiClear 1.47 clearing method developed by the SunJin Lab ( ; ).

Techniques: Recombinant, Fluorsave, Software, Microscopy