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48 pin contact microarrayer  (Bio-Rad)


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    Structured Review

    Bio-Rad 48 pin contact microarrayer
    48 Pin Contact Microarrayer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1859 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chipwriter+pro+microarray+printer/Protease/pmc03790292-528-19-24
    Average 96 stars, based on 1859 article reviews
    48 pin contact microarrayer - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: Damnacanthal Suppresses Breast Cancer Cells by Inducing Apoptosis and Cell Cycle Arrest via NF ‐ ĸB Signaling
    Article Snippet: BCA‐protein assay kit, protease inhibitor, polyvinylidene difluoride (PVDF) membrane, nonfat dry milk, and precision plus protein dual color standard were purchased from Bio‐Rad (Hercules, CA, USA).

    Article Title: Damnacanthal Suppresses Breast Cancer Cells by Inducing Apoptosis and Cell Cycle Arrest via NF-ĸB Signaling.
    Article Snippet: BCA- protein assay kit, protease inhibitor, polyvinylidene difluoride (PVDF) membrane, nonfat dry milk, and precision plus protein dual color standard were purchased from BioRad (Hercules, CA, USA).

    Activity Assay:

    Article Title: GRASP55 maintains lysosome function by controlling sorting of lysosomal enzymes at the Golgi.
    Article Snippet: .. Magic Red lysosomal protease activity assay Intracellular lysosomal protease activity was measured in whole living cells using the Magic Red Cathepsin B protease activity Kit (#ICT937, Bio-Rad) according to manufacturer’s instructions. .. In brief, cells were labelled with the Magic Red substrate and Hoechst (1 μg/ml) for 1 h at 37 °C, and then fixed with 4% formaldehyde in PBS for 10 min at room temperature and mounted on glass slides with Fluoromount-G mounting medium (#00-4958-02, Invitrogen).

    Lysis:

    Article Title: Single-Cell Triomics Analysis of Tumor Cells Infiltrating Patient-Derived Breast Cancer Scaffolds
    Article Snippet: .. Subsequently, cell lysis, reverse transcription, and protease treatment were performed on a T-100 thermal cycler (Bio-Rad, Hercules, CA) at 50°C for 60 minutes followed by 80°C for 10 minutes. .. Custom DNA and RNA panels (Mission Bio, Inc.) were used.

    Reverse Transcription:

    Article Title: Single-Cell Triomics Analysis of Tumor Cells Infiltrating Patient-Derived Breast Cancer Scaffolds
    Article Snippet: .. Subsequently, cell lysis, reverse transcription, and protease treatment were performed on a T-100 thermal cycler (Bio-Rad, Hercules, CA) at 50°C for 60 minutes followed by 80°C for 10 minutes. .. Custom DNA and RNA panels (Mission Bio, Inc.) were used.

    Protein Concentration:

    Article Title: Beyond the surface: plasmalogens are dispensable for retinal integrity and fertility in the mouse
    Article Snippet: Microsomal fractions were prepared by homogenizing the eyes in 200 μl 0.1 mM Tris HCl and 0.25 M sucrose (pH 7.2), with a cocktail of protease inhibitors (aprotinin 50 μg/ml; pepstatin A 50 μM; trypsin inhibitor 5 mg/ml; iodoacetamide 100 mM; leupeptin 50 μg/ml), using an Ultra Turrax (IKA, Staufen, Germany) followed by centrifugation at 3,000 x g for 10 min at 4°C. .. The supernatant was centrifuged at 20,000 x g for 30 min at 4°C, and the pellet was resuspended in 30 μl Tris-HCl-sucrose buffer, including protease inhibitors, and protein concentration was quantified by Bradford assay (Bio-Rad, Hercules, California, USA, #5000006), using BSA as standard. ..

    Article Title: Accelerated reprogramming of hiPSCs into functional brain endothelial-like cells using multiplexed CRISPR activation.
    Article Snippet: .. Cells were lysed with 1 RIPA buffer with protease inhibitors (BioRad, Sigma #20- 188, Roche Diagnostics, #11697498001) and a Quantipro BCA assay (Sigma, #QPBCA) was performed to quantify the protein concentration for each sample. .. Western blot assays were performed using the Wes System (Protein Simple, #004-600) and samples were diluted to 1.2 mg/mL in sample buffer and a fluorescent master mix according to the manufacturer’s protocol (Protein Simple, # PS-ST03, #042-195).

    Bradford Assay:

    Article Title: Beyond the surface: plasmalogens are dispensable for retinal integrity and fertility in the mouse
    Article Snippet: Microsomal fractions were prepared by homogenizing the eyes in 200 μl 0.1 mM Tris HCl and 0.25 M sucrose (pH 7.2), with a cocktail of protease inhibitors (aprotinin 50 μg/ml; pepstatin A 50 μM; trypsin inhibitor 5 mg/ml; iodoacetamide 100 mM; leupeptin 50 μg/ml), using an Ultra Turrax (IKA, Staufen, Germany) followed by centrifugation at 3,000 x g for 10 min at 4°C. .. The supernatant was centrifuged at 20,000 x g for 30 min at 4°C, and the pellet was resuspended in 30 μl Tris-HCl-sucrose buffer, including protease inhibitors, and protein concentration was quantified by Bradford assay (Bio-Rad, Hercules, California, USA, #5000006), using BSA as standard. ..

    Article Title: IL-10-STAT3 axis preserves epithelial mitochondrial homeostasis during bacterial challenge.
    Article Snippet: .. Upon completion of the assay, cells were lysed using NP-40 buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% NP-40, 1 mM sodium orthovanadate) containing a protease inhibitor cocktail and total protein content per well was quantified using the Bradford assay (Bio-Rad, CA). ..

    Article Title: IL-10-STAT3 axis preserves epithelial mitochondrial homeostasis during bacterial challenge
    Article Snippet: .. Upon completion of the assay, cells were lysed using NP-40 buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% NP-40, 1 mM sodium orthovanadate) containing a protease inhibitor cocktail and total protein content per well was quantified using the Bradford assay (Bio-Rad, CA). ..

    BIA-KA:

    Article Title: Accelerated reprogramming of hiPSCs into functional brain endothelial-like cells using multiplexed CRISPR activation.
    Article Snippet: .. Cells were lysed with 1 RIPA buffer with protease inhibitors (BioRad, Sigma #20- 188, Roche Diagnostics, #11697498001) and a Quantipro BCA assay (Sigma, #QPBCA) was performed to quantify the protein concentration for each sample. .. Western blot assays were performed using the Wes System (Protein Simple, #004-600) and samples were diluted to 1.2 mg/mL in sample buffer and a fluorescent master mix according to the manufacturer’s protocol (Protein Simple, # PS-ST03, #042-195).

    Protease Inhibitor:

    Article Title: IL-10-STAT3 axis preserves epithelial mitochondrial homeostasis during bacterial challenge.
    Article Snippet: .. Upon completion of the assay, cells were lysed using NP-40 buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% NP-40, 1 mM sodium orthovanadate) containing a protease inhibitor cocktail and total protein content per well was quantified using the Bradford assay (Bio-Rad, CA). ..

    Article Title: IL-10-STAT3 axis preserves epithelial mitochondrial homeostasis during bacterial challenge
    Article Snippet: .. Upon completion of the assay, cells were lysed using NP-40 buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% NP-40, 1 mM sodium orthovanadate) containing a protease inhibitor cocktail and total protein content per well was quantified using the Bradford assay (Bio-Rad, CA). ..



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    A representative concentration profile showing the response of an AIR <t>microarray</t> to varying concentrations of Tissue Factor (TF) in buffer containing a background of 10 mg/mL BSA. The lower limit of detection (LOD) for this analyte was determined to be below 1 pg/mL, as our unexposed control chip has zero normalized reflectance on this scale.
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    Image Search Results


    A representative concentration profile showing the response of an AIR microarray to varying concentrations of Tissue Factor (TF) in buffer containing a background of 10 mg/mL BSA. The lower limit of detection (LOD) for this analyte was determined to be below 1 pg/mL, as our unexposed control chip has zero normalized reflectance on this scale.

    Journal: Biosensors & bioelectronics

    Article Title: Analysis of Inflammatory Biomarkers by Arrayed Imaging Reflectometry

    doi: 10.1016/j.bios.2011.02.025

    Figure Lengend Snippet: A representative concentration profile showing the response of an AIR microarray to varying concentrations of Tissue Factor (TF) in buffer containing a background of 10 mg/mL BSA. The lower limit of detection (LOD) for this analyte was determined to be below 1 pg/mL, as our unexposed control chip has zero normalized reflectance on this scale.

    Article Snippet: Antibody arrays for all experiments except those involving detection in 20% serum were fabricated using a ChipWriter Pro microarray printer (Virtek, Waterloo, Ontario CA), with probes spotted using stealth 2.5XB slotted pins (TeleChem International, Sunnyvale, CA) in a 70% RH atmosphere.

    Techniques: Concentration Assay, Microarray, Control