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DIAGENODE DIAGNOSTICS ideal chip-seq kit transcription factors kit
Reagents and tools table
Ideal Chip Seq Kit Transcription Factors Kit, supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chip-seq+kit/ideal+chip+seq+kit+for+transcription+factors/pmc11628624-101-0-8
Average 90 stars, based on 1 article reviews
ideal chip-seq kit transcription factors kit - by Bioz Stars, 2026-10
90/100 stars

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1) Product Images from "Reciprocal inhibition of NOTCH and SOX2 shapes tumor cell plasticity and therapeutic escape in triple-negative breast cancer"

Article Title: Reciprocal inhibition of NOTCH and SOX2 shapes tumor cell plasticity and therapeutic escape in triple-negative breast cancer

Journal: EMBO Molecular Medicine

doi: 10.1038/s44321-024-00161-8

Reagents and tools table
Figure Legend Snippet: Reagents and tools table

Techniques Used: Recombinant, Plasmid Preparation, Binding Assay, Polymer, SYBR Green Assay, Transfection, Protease Inhibitor, Reporter Assay, Gel Purification, Purification, Ligation, Software

Related Articles

other:

Article Title: RNA m 6 A dynamics promote transcription and RNA stability of bivalent genes during iPSC-induced generation of human lung progenitors.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Dorsomorphin MedChem Express HY-13418A Retinoic acid (Vitamin A) Selleck Chemicals S1653 Polyethylenimine, Linear, MW 25000, Transfection Grade (PEI 25K) Polysciences 23966 VeZol Reagent Vazyme R411-02 actinomycin D Sigma-Aldrich A9415 RNAClean XP beads Beckman Coulter A63987 RiboLock RNase inhibitor Thermo Fisher Scientific EO0382 Pierce Protein A/G Magnetic Beads Thermo Fisher Scientific 88803 Proteinase K Invitrogen 25530049 sodium acetate Sigma-Aldrich S7899 linear acrylamide Thermo Fisher Scientific AM9520 AMPure XP beads Beckman Coulter A63881 IGEPAL CA-630 Sigma-Aldrich I8896 DNase Roche 0476728001 ChamQ SYBR qPCR Master Mix Vazyme Q311-03 Fetal Bovine Serum ExCell FSS500 IC FIX BUFFER Thermo Fisher Scientific 00-8222-49 Triton X-100 Sigma-Aldrich T8787-100ML Critical commercial assays Primescript RT reagent kit TAKARA RR037A NEB Next rRNA depletion kit NEB E7400L SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian TAKARA 634413 PolyATtract mRNA Isolation System III Promega Z5300 iDeal ChIP-seq for Histomes kit Diagenode C01010051 Deposited data picoMeRIP-seq (Raw and analyzed data) This paper GEO: GSE283697 SUZ12 ChIP-seq in human H1-hESC ENCODE ENCSR000ATS EZH2 ChIP-seq in human H1-hESC ENCODE ENCSR000ASY Human H3K27me3 ChIP-seq in HUES64 ENCODE ENCSR634MAK Human H3K27me3 ChIP-seq in endodermal cell ENCODE ENCSR273IYV Human H3K4me3 ChIP-seq in HUES64 ENCODE ENCSR894OYM Human H3K4me3 ChIP-seq in endodermal cell ENCODE ENCSR446ZCY RBM15B iCLIP-seq in HEK293T cell GEO GSM2064711 YTHDC1 ChIP-seq in human H1-hESC GEO GSM7029340 Experimental models: Cell lines Human peripheral blood mononuclear cell-derived iPSCs From Prof. Guangjin Pan (Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences) PB004 HEK293T ATCC CRL-3216 Oligonucleotides Primers for RT-qPCR, see Table S1 This paper N/A Primers for MeRIP-qPCR and RIP-qPCR, see Table S1 This paper N/A Primers for ChIP-qPCR, see Table S1 This paper N/A Primers for TBP, see Table S1 This paper N/A (Continued on next page) Cell Reports 44, 115802, June 24, 2025 19

Article Title: Age-Dependent Histone Deacetylase 3 Regulation by βA3/A1-Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age-Related Macular Degeneration.
Article Snippet: Chromatin immunoprecipitation (ChIP) was performed using iDeal ChIP- seq kit (Diagenode, Cat #: C01010051) on 25 μg of sheared chromatin using 5 μL of H3K27- Ac antibody (Abcam, Cat #: ab- 4729).

Article Title: Epigenetic Regulation of Chondrogenesis: JMJD3 and UTX as Key Targets for Gene-Modified Mesenchymal Stem Cell Therapy in Cartilage Tissue Engineering
Article Snippet: The chromatin was prepared using the iDeal ChIP-seq kit for Histones (Diagenode Cat# C01010059).

Chromatin Immunoprecipitation:

Article Title:
Article Snippet: .. Chromatin immunoprecipitation was performed following iDeal ChIP-seq kit for Transcription Factors (Diagenode, Cat. No. C01010055) using 7 cycles for chromatin shearing on a Bioruptor Pico sonicator (Diagenode, Cat. No. B01060001). .. Actin filaments were stained using Alexa Fluor-conjugated phalloidin (Invitrogen; 50 nM in PBS) for 15 min exactly at RT.

Article Title: SOX2 utilizes FOXA1 as a heteromeric transcriptional partner to drive proliferation in therapy-resistant prostate cancer
Article Snippet: Both CWR-R1 and NCI-H660 cells were fixed in 1% w/v formaldehyde (ThermoFisher, Cat: ICN19404780) for 8 minutes followed by quenching in 125mM glycine (ThermoFisher, Cat: A13816.36) for 5 minutes shaking at room temperature. .. Following fixation and quenching, we performed chromatin immunoprecipitation, size selection, and purification using the Diagenode iDeal ChIP kit for Transcription Factors (Diagenode, Cat: C01010055) according to manufacturer’s protocol. .. AR was immunoprecipitated with the kit magnetic beads conjugated to polyclonal rabbit anti-AR (PG-21) antibody (Millipore, Cat: 06-680) or the species-matched rabbit IgG control antibody (Cell Signaling, Cat: 2729).

Article Title: The Non-Coding Regulatory Variant rs2863002 at chr11p11.2 Increases Neuroblastoma Risk by Affecting HSD17B12 Expression and Lipid Metabolism.
Article Snippet: .. ChIP experiments were performed by using iDeal ChIP-seq kit for Transcription Factors (C01010055, Diagenode) according to manufacturer instructions. ..

Article Title: The Non-Coding Regulatory Variant rs2863002 at chr11p11.2 Increases Neuroblastoma Risk by Affecting HSD17B12 Expression and Lipid Metabolism.
Article Snippet: .. ChIP reactions were performed using iDeal ChIP-seq kit for Transcription Factors (C01010055, Diagenode) according to manufacturer instructions, as described above for ChIP-seq experiments. ..

Article Title: RNA m 6 A dynamics promote transcription and RNA stability of bivalent genes during iPSC-induced generation of human lung progenitors.
Article Snippet: ChIP experiments were undertaken using iDeal ChIP-seq for Histones kit (Diagenode, C01010051) according to manufacturer’s pro- tocol. .. ChIP experiments were undertaken using iDeal ChIP-seq for Histones kit (Diagenode, C01010051) according to manufacturer’s pro- tocol. .. YTHDC1 (Proteintech, 29441, 1:100), SUZ12 (Cell Signaling Technology, 3737S, 1:100) and H3K27me3 (Abcam, 192985, 1: 300) antibodies were used for immunoprecipitation.

Size Selection:

Article Title: SOX2 utilizes FOXA1 as a heteromeric transcriptional partner to drive proliferation in therapy-resistant prostate cancer
Article Snippet: Both CWR-R1 and NCI-H660 cells were fixed in 1% w/v formaldehyde (ThermoFisher, Cat: ICN19404780) for 8 minutes followed by quenching in 125mM glycine (ThermoFisher, Cat: A13816.36) for 5 minutes shaking at room temperature. .. Following fixation and quenching, we performed chromatin immunoprecipitation, size selection, and purification using the Diagenode iDeal ChIP kit for Transcription Factors (Diagenode, Cat: C01010055) according to manufacturer’s protocol. .. AR was immunoprecipitated with the kit magnetic beads conjugated to polyclonal rabbit anti-AR (PG-21) antibody (Millipore, Cat: 06-680) or the species-matched rabbit IgG control antibody (Cell Signaling, Cat: 2729).

Purification:

Article Title: SOX2 utilizes FOXA1 as a heteromeric transcriptional partner to drive proliferation in therapy-resistant prostate cancer
Article Snippet: Both CWR-R1 and NCI-H660 cells were fixed in 1% w/v formaldehyde (ThermoFisher, Cat: ICN19404780) for 8 minutes followed by quenching in 125mM glycine (ThermoFisher, Cat: A13816.36) for 5 minutes shaking at room temperature. .. Following fixation and quenching, we performed chromatin immunoprecipitation, size selection, and purification using the Diagenode iDeal ChIP kit for Transcription Factors (Diagenode, Cat: C01010055) according to manufacturer’s protocol. .. AR was immunoprecipitated with the kit magnetic beads conjugated to polyclonal rabbit anti-AR (PG-21) antibody (Millipore, Cat: 06-680) or the species-matched rabbit IgG control antibody (Cell Signaling, Cat: 2729).



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