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CombiMatrix array cgh
Summary of molecular profiling analyses from various CLIA certified methods in a patient with malignant phyllodes sarcoma
Array Cgh, supplied by CombiMatrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cgh+array/array+cgh/pmc03751902-83-3-14
Average 90 stars, based on 1 article reviews
array cgh - by Bioz Stars, 2026-09
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Article Title: Comprehensive characterization of malignant phyllodes tumor by whole genomic and proteomic analysis: biological implications for targeted therapy opportunities

Journal: Orphanet Journal of Rare Diseases

doi: 10.1186/1750-1172-8-112

Summary of molecular profiling analyses from various CLIA certified methods in a patient with malignant phyllodes sarcoma
Figure Legend Snippet: Summary of molecular profiling analyses from various CLIA certified methods in a patient with malignant phyllodes sarcoma

Techniques Used: Sequencing, Hybridization, Immunohistochemistry, Staining

Imaging studies, histopathology and FISH studies: UA and B: CT of the chest showing metastatic masses in the lung. C Hematoxylin and Eosin stained section of the tumor from the right mastectomy shows a malignant sarcoma/malignant phyllodes tumor that has high stromal cellularity and a mitotic index of 11 per 10 high power fields. D HER - 2 / neu by Fluorescence in Situ Hybridization (FISH) HER-2 gene amplification was assessed utilizing the PathVysion assay (Vysis Corp., Downers Grove, Illinois). The identification probes for the HER-2 (SpectrumOrange) and alpha satellite DNA sequence at the centromeric region of chromosome 17 (SpectrumGreen) were hybridized according to the manufacturer’s guidelines. At least twenty non-overlapping nuclei containing at least one orange and one green signal were enumerated. The ratio of orange signals (HER-2 gene) to green signals (chromosome 17) was calculated. A ratio greater than or equal to 2.0 is considered as amplified based on the FDA approval in this kit. The CAP HER-2 consensus conference 2002 suggested that a ratio of 1.8-2.2 be considered as borderline.
Figure Legend Snippet: Imaging studies, histopathology and FISH studies: UA and B: CT of the chest showing metastatic masses in the lung. C Hematoxylin and Eosin stained section of the tumor from the right mastectomy shows a malignant sarcoma/malignant phyllodes tumor that has high stromal cellularity and a mitotic index of 11 per 10 high power fields. D HER - 2 / neu by Fluorescence in Situ Hybridization (FISH) HER-2 gene amplification was assessed utilizing the PathVysion assay (Vysis Corp., Downers Grove, Illinois). The identification probes for the HER-2 (SpectrumOrange) and alpha satellite DNA sequence at the centromeric region of chromosome 17 (SpectrumGreen) were hybridized according to the manufacturer’s guidelines. At least twenty non-overlapping nuclei containing at least one orange and one green signal were enumerated. The ratio of orange signals (HER-2 gene) to green signals (chromosome 17) was calculated. A ratio greater than or equal to 2.0 is considered as amplified based on the FDA approval in this kit. The CAP HER-2 consensus conference 2002 suggested that a ratio of 1.8-2.2 be considered as borderline.

Techniques Used: Imaging, Histopathology, Staining, Fluorescence, In Situ Hybridization, Amplification, Sequencing

Related Articles

other:

Article Title: Assessment of Comparative Genomic Hybridization Experiment by an in situ Synthesized CombiMatrix Microarray with Yersinia pestis Vaccine Strain EV76 DNA
Article Snippet: Through the analysis of the data generated from a CombiMatrix comparative genomic hybridization (CGH) array, we found that all probe Tms should be within a narrow range, Tm and hybridization temperatures should be balanced, and proper target genomic DNA fragments might be in the range from 200 to 800 bp or in a shorter range.

Article Title: Structural Chromosome Abnormalities Associated with Obesity: Report of Four New subjects and Review of Literature
Article Snippet: Array CGH studies for Subjects 1, 2 and 3 were performed at CombiMatrix TM (Irvine, CA) while Subject 4 was studied at The Children’s Mercy Hospital Microarray Laboratory (Kansas City, MO).

Article Title: Comprehensive characterization of malignant phyllodes tumor by whole genomic and proteomic analysis: biological implications for targeted therapy opportunities
Article Snippet: Whole-genome array-based comparative genomic hybridization (array CGH) was performed by using the CLIA-certified DNAarrayTM (CombiMatrix Diagnostics, Irvine, CA).

Article Title: Presented Abstracts from the Thirtieth Annual Education Conference of the National Society of Genetic Counselors (San Diego, CA, October 2011)
Article Snippet: Presented Abstracts from the Thirtieth Annual Education Conference of the National Society of Genetic Counselors (San Diego, CA, October 2011) Carrie L. Atzinger & Ashley Melvin & Brandie Heald Abstracts have been arranged by the Abstract Workgroup into the following categories:s have been arranged by the Abstract Workgroup into the following categories: I.. Award Papers II.. Adult III.

Article Title: Comprehensive characterization of malignant phyllodes tumor by whole genomic and proteomic analysis: biological implications for targeted therapy opportunities
Article Snippet: Whole-genome array-based comparative genomic hybridization (array CGH) was performed by using the DNAarrayTM (CombiMatrix Diagnostics, Irvine, CA).

Hybridization:

Article Title: Comparison of array comparative genomic hybridization (aCGH) to FISH and cytogenetics in prognostic evaluation of chronic lymphocytic leukemia
Article Snippet: Chronic lymphocytic leukemia (CLL) is one of the most commonly encountered hematologic neoplasms in adults.. It has a highly variable clinical course ranging from indolent to rapidly progressive disease.. In the past decade, therapeutic approaches have changed considerably with the identification of different subgroups of CLL, the addition of new and more effective chemotherapeutic agents, and new treatment protocols.

Diagnostic Assay:

Article Title: The HemeScan test for genomic prognostic marker assessment in chronic lymphocytic leukemia.
Article Snippet: The HemeScan test for genomic prognostic marker assessment in chronic lymphocytic leukemia Shelly R Gunn, Mansoor S Mohammed, Clemens HM Mellink, Lynne V Abruzzo & Ryan S Robetorye To cite this article: Shelly R Gunn, Mansoor S Mohammed, Clemens HM Mellink, Lynne V Abruzzo & Ryan S Robetorye (2008) The HemeScan test for genomic prognostic marker assessment in chronic lymphocytic leukemia, Expert Opinion on Medical Diagnostics, 2:6, 731-740 To link to this article: http://dx.doi.org/10.1517/17530059.2.6.731

BAC Assay:

Article Title: The HemeScan test for genomic prognostic marker assessment in chronic lymphocytic leukemia.
Article Snippet: The HemeScan test for genomic prognostic marker assessment in chronic lymphocytic leukemia Shelly R Gunn, Mansoor S Mohammed, Clemens HM Mellink, Lynne V Abruzzo & Ryan S Robetorye To cite this article: Shelly R Gunn, Mansoor S Mohammed, Clemens HM Mellink, Lynne V Abruzzo & Ryan S Robetorye (2008) The HemeScan test for genomic prognostic marker assessment in chronic lymphocytic leukemia, Expert Opinion on Medical Diagnostics, 2:6, 731-740 To link to this article: http://dx.doi.org/10.1517/17530059.2.6.731



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