g418 (Thermo Fisher)

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Thermo Fisher g418

Stability of sfl genomes during continuous cell culture. (A) Six different cell lines were continuously cultured in the presence of <t>G418.</t> At the indicated time points (days [d]), cells were lysed, total RNA was prepared, and 4 μg was analyzed by Northern blotting. For comparison, given amounts of RNA containing subgenomic replicon transcribed in vitro were analyzed in parallel. To allow detection of sequence variants and of smaller HCV RNA pieces, the entire blot was hybridized under low-stringency conditions. (B) Genetic drift of the sfl genome in cell line 21-5. Cells were continuously cultured, and after 57 passages (corresponding to ∼6 months), total RNA was prepared. The coding region of the HCV polyprotein was amplified by long-distance RT-PCR in two overlapping fragments shown as bars below the ORF. For each fragment, two independent clones were sequenced. The boundary of the sequenced clones is indicated by a dashed vertical line. Amino acid substitutions are drawn as vertical lines at their respective positions within the HCV polyprotein. The hypervariable region 1 (HVR1) present at the N terminus of E2 is depicted as a shaded area. Six mutations were conserved between both sequenced clones, and they are indicated (stars).

G418, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+transcriptome+sequencing+analysis/G418+DISULFATE+CELL+CULTURE+RE/pmc00136109-62-9-11
Average 99 stars, based on 1 article reviews

g418 - by Bioz Stars, 2026-10

99/100 stars

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1) Product Images from "Persistent and Transient Replication of Full-Length Hepatitis C Virus Genomes in Cell Culture"

Article Title: Persistent and Transient Replication of Full-Length Hepatitis C Virus Genomes in Cell Culture

Journal:

doi: 10.1128/JVI.76.8.4008-4021.2002

Stability of sfl genomes during continuous cell culture. (A) Six different cell lines were continuously cultured in the presence of G418. At the indicated time points (days [d]), cells were lysed, total RNA was prepared, and 4 μg was analyzed by Northern blotting. For comparison, given amounts of RNA containing subgenomic replicon transcribed in vitro were analyzed in parallel. To allow detection of sequence variants and of smaller HCV RNA pieces, the entire blot was hybridized under low-stringency conditions. (B) Genetic drift of the sfl genome in cell line 21-5. Cells were continuously cultured, and after 57 passages (corresponding to ∼6 months), total RNA was prepared. The coding region of the HCV polyprotein was amplified by long-distance RT-PCR in two overlapping fragments shown as bars below the ORF. For each fragment, two independent clones were sequenced. The boundary of the sequenced clones is indicated by a dashed vertical line. Amino acid substitutions are drawn as vertical lines at their respective positions within the HCV polyprotein. The hypervariable region 1 (HVR1) present at the N terminus of E2 is depicted as a shaded area. Six mutations were conserved between both sequenced clones, and they are indicated (stars).

Figure Legend Snippet: Stability of sfl genomes during continuous cell culture. (A) Six different cell lines were continuously cultured in the presence of G418. At the indicated time points (days [d]), cells were lysed, total RNA was prepared, and 4 μg was analyzed by Northern blotting. For comparison, given amounts of RNA containing subgenomic replicon transcribed in vitro were analyzed in parallel. To allow detection of sequence variants and of smaller HCV RNA pieces, the entire blot was hybridized under low-stringency conditions. (B) Genetic drift of the sfl genome in cell line 21-5. Cells were continuously cultured, and after 57 passages (corresponding to ∼6 months), total RNA was prepared. The coding region of the HCV polyprotein was amplified by long-distance RT-PCR in two overlapping fragments shown as bars below the ORF. For each fragment, two independent clones were sequenced. The boundary of the sequenced clones is indicated by a dashed vertical line. Amino acid substitutions are drawn as vertical lines at their respective positions within the HCV polyprotein. The hypervariable region 1 (HVR1) present at the N terminus of E2 is depicted as a shaded area. Six mutations were conserved between both sequenced clones, and they are indicated (stars).

Techniques Used: Cell Culture, Northern Blot, In Vitro, Sequencing, Amplification, Reverse Transcription Polymerase Chain Reaction, Clone Assay

Stable Transfection:

Article Title: Drosophila Abi maintains blood cell homeostasis by promoting clathrin-mediated endocytosis of Notch
Article Snippet: For expression in Escherichia coli , a cDNA encoding NICD (amino acid residues 1765–2703) was PCR-amplified from genomic DNA isolated from UAS-N transgenic flies and cloned into the 6xHis tag vector pCold-TF (pCold-His6-TF, Takara) to produce pCold-His 6 -TF-NICD . .. S2 cells stably expressing the Myc-Ser fusion under the control of an actin promoter (S2S cells) were generated by transfecting the Ac5-Myc-Ser-STABLE1-Neo vector and selecting cells in 2 mg/ml G418 (Invitrogen) as previously described ( ). .. These cells were maintained at 25°C in Shields and Sang M3 Insect Medium (USBiological Life Sciences) supplemented with 10% heat-inactivated (30 min, 56°C) fetal bovine serum, 1 g/liter yeast extract, 2.5 g/liter Bacto-peptone, 100 U/ml penicillin, and 100 μg/ml streptomycin (Gibco).

Expressing:

Control:

Generated:

Polymerase Chain Reaction:

Article Title: Endocytosis of the Saccharomyces cerevisiae Jen1 monocarboxylate-proton symporter under mildly alkaline conditions: a mechanism to prevent metabolite loss?
Article Snippet: All the S. cerevisiae strains generated in this work were constructed as follows: firstly, DNA fragments were amplified by PCR (Accuzyme DNA Polymerase, Bioline, or Supreme NZYProof DNA Polymerase, Nzytech) with specific oligonucleotides (listed in Table ) using yeast genomic DNA or plasmid DNA. .. The resulting PCR products were introduced in S. cerevisiae cells, by following the polyethylene glycol (PEG)/lithium acetate (LiAc) protocol [ ], and the resulting transformants were selected on geneticin (G418 disulfate, Alfa Aesar; J62671) or hygromycin B (Alfa Aesar; J60681) according to the resistance (R) marker present (geneticin for kan R and hygromycin for hgh R). ..

Marker:

Transduction:

Article Title: ZEB1, a novel junctional adhesion molecule A regulator, impacts sensitivity of pancreatic cancer-associated fibroblasts to reovirus
Article Snippet: .. Clonal ZEB1 KO cells were transduced with this vector and selected and subsequently cultured with puromycin (2 μg/mL; Sigma-Aldrich) or G418 (400 μg/mL; Thermo Fisher Scientific). ..

Plasmid Preparation:

Cell Culture:

Recombinant:

Article Title: A human CAGinSTEM platform for decoding HTT repeats’ somatic instability links CAG interruption to HD pathology in neurons
Article Snippet: HiFi Cas9 Nuclease V3 (IDT # 1081060) and sgRNA RNP complex using Nucleofector kit (Lonza), in Nucleofector II (Amaxa biosystems) using B-016 program. .. After nucleofection, cells were plated in mTeSR media with 10 μM RI (Y-27632, provided by CHDI foundation) and 1 μM of SCR7 (TOCRIS) for 24 h. Two days after nucleofection, cells were daily treated with 0.1 mg/mL G418 (ThermoFisher) for 5 days to select and isolate recombinant cells. ..

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