cell stacks (Corning Life Sciences)

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Corning Life Sciences cell stacks
Cell Stacks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+stack/cellstack/pm40259021-42-18-20
Average 90 stars, based on 1 article reviews

cell stacks - by Bioz Stars, 2026-09

90/100 stars

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Cell Culture:

Article Title: Small molecule induced STING degradation facilitated by the HECT ligase HERC4.
Article Snippet: .. Briefly, 2.1 × 107 HEK293T cells were seeded into CellSTACK (Corning, Cat#3391) cell culture chambers and transfectedwith the sgRNA library plasmid mix together with ready-to-use lentiviral packaging plasmid mix (Cellecta, Cat#CPCP-K2A) 24 h after seeding using the Trans-IT transfection reagent (Mirus, Cat#MIR2304). .. Viral particles were harvested 72 h post-transfection and quantified using the Lenti-X qPCR kit (Clonetech, Cat#631235).

Article Title: Limitations and challenges in the characterization of extracellular vesicles from stem cells and serum.
Article Snippet: .. All donors have signed the informed consent forms. hUCESC cell culture and CM harvest hUCESC were cultured in cell stacks (Corning, NY) at 3000 cells/cm2 in DMEM-F12 with 10% fetal bovine serum (FBS), penicillin, and streptomycin (Corning) in an air-CO2 (95–5%) atmosphere at 37 °C in a Celsius 2007 cell incubator (Memmert GmbH, Germany). .. The cells were grown until 80% of cell confluence, cell culture media was discarded, and cells were washed three times with phosphate-buffered saline (PBS) (Lonza, Spain).

Plasmid Preparation:

Transfection:

other:

Article Title: Sabin inactivated polio vaccine upstream process development using fixed-bed bioreactor technology
Article Snippet: For each experiment a cryo-vial of this Vero cell bank was thawed and expanded in T-flasks, followed by Cell Stacks (Corning), until sufficient viable cells were obtained to inoculate the bioreactor at 5000 cells/cm 2 in the 2.4 m 2 , 30 m 2 , and 150 m 2 scale-X fixed-bed bioreactors (Univercells Technologies).

Article Title: Sabin inactivated polio vaccine upstream process development using fixed-bed bioreactor technology.
Article Snippet: For each experiment a cryo-vial of this Vero cell bank was thawed and expanded in T-flasks, followed by Cell Stacks (Corning), until sufficient viable cells were obtained to inoculate the bioreactor at 5000 cells/cm2 in the 2.4 m2, 30 m2, and 150 m2 scale-X fixed-bed bioreactors (Univercells Technologies).

Modification:

Article Title: Comparability exercise of critical quality attributes of clinical-grade human mesenchymal stromal cells from the Wharton's jelly: single-use stirred tank bioreactors versus planar culture systems.
Article Snippet: .. Each cryovial was thawed and plated onto 2-layer CellSTACK (Corning, Corning, NY, USA) at a cell density of 1.6 £ 103 2.1 £ 103 cells/cm2 using high-glucose (4.5 g/L) GlutaMAX Dulbecco’s Modified Eagle Medium (DMEM-HG; Gibco, Life Technologies, Waltham, MA, USA) and supplemented with 10% human serum B (hSerB; Banc de Sang i Teixits). ..

Incubation:

Article Title: Evaluation of Genotoxic Effects of N-Methyl-N-Nitroso-Urea and Etoposide on the Differentiation Potential of MSCs from Umbilical Cord Blood and Bone Marrow
Article Snippet: .. Cells were expanded without dexamethasone in a closed system applying cell stacks (Corning), incubated at 37 °C in 5% CO 2 in a humidified atmosphere. .. After reaching 80% confluency, cells were detached with 0.25% trypsin (Lonza) and re-plated 1:3.

Article Title: Evaluation of Genotoxic Effects of N-Methyl-N-Nitroso-Urea and Etoposide on the Differentiation Potential of MSCs from Umbilical Cord Blood and Bone Marrow
Article Snippet: A total of 5–7 × 10 6 CB-MNC/mL was cultured in low-glucose Dulbecco’s modified Eagle’s medium (DMEM) (Lonza, Basel, Switzerland) with 30% fetal calf serum (FCS) (Perbio, Bonn, Germany), 10 –7 M dexamethasone (Sigma-Aldrich, St-Louis, MO, USA), and penicillin/streptomycin (Lonza) until detection of adherent growing colonies. .. Cells were expanded without dexamethasone in a closed system applying cell stacks (Corning, New York, NY, USA), incubated at 37 °C in 5% CO 2 in a humidified atmosphere. .. After reaching 80% confluency, cells were detached with 0.25% trypsin (Lonza) and re-plated 1:3.

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