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cdna microarray genechip human genome hgu133a  (Thermo Fisher)


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    Thermo Fisher cdna microarray genechip human genome hgu133a
    Cdna Microarray Genechip Human Genome Hgu133a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+microarray+system/seebluetm+plus2+pre+stained+protein+standard/pm40361356-70-0-7
    Average 90 stars, based on 1 article reviews
    cdna microarray genechip human genome hgu133a - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Molecular Weight:

    Article Title: A Quantitative High-Throughput Screen Identifies Compounds that Upregulate the p53 Isoform Δ133p53α and Inhibit Cellular Senescence
    Article Snippet: Δ133p53α, a human/primate-specific p53 protein isoform, delays or inhibits the induction of the cellular senescence and associated secretory phenotype (SASP) in various types of human cells, including astrocytes from neurodegenerative diseases, Hutchinson−Gilford progeria syndrome (HGPS) fibroblasts, and exhausted CD8+ T-cells.. A major regulatory mechanism of Δ133p53α expression in these human cells is protein degradation via chaperone-assisted selective autophagy (CASA).. In this study, we describe a novel cell-based quantitative high-throughput screening (qHTS) assay using fluorescently labeled Δ133p53α to screen large chemical libraries for the identification of compounds that upregulate the Δ133p53α protein level. qHTS enabled the comprehensive and reliable profiling of over 10,000 small-molecule compounds.

    Article Title: Purification and characterization of recombinant neuraminidase as a potentially broadly protective influenza virus vaccine candidate.
    Article Snippet: .. SeeBlue Plus2 Prestained Protein Standard marker (Thermo Fisher Scientific) was used as molecular weight marker for SDS-PAGEs and Western blots. ..

    Article Title: KRAS inhibition reverses chemotherapy resistance promoted by therapy-induced senescence-like in pancreatic ductal adenocarcinoma
    Article Snippet: .. The estimation of bands was performed using a prestained protein ladder (SeeBlue Plus2, Thermo Fisher Scientific) as a molecular weight marker. ..

    Marker:

    Article Title: A Quantitative High-Throughput Screen Identifies Compounds that Upregulate the p53 Isoform Δ133p53α and Inhibit Cellular Senescence
    Article Snippet: Δ133p53α, a human/primate-specific p53 protein isoform, delays or inhibits the induction of the cellular senescence and associated secretory phenotype (SASP) in various types of human cells, including astrocytes from neurodegenerative diseases, Hutchinson−Gilford progeria syndrome (HGPS) fibroblasts, and exhausted CD8+ T-cells.. A major regulatory mechanism of Δ133p53α expression in these human cells is protein degradation via chaperone-assisted selective autophagy (CASA).. In this study, we describe a novel cell-based quantitative high-throughput screening (qHTS) assay using fluorescently labeled Δ133p53α to screen large chemical libraries for the identification of compounds that upregulate the Δ133p53α protein level. qHTS enabled the comprehensive and reliable profiling of over 10,000 small-molecule compounds.

    Article Title: Purification and characterization of recombinant neuraminidase as a potentially broadly protective influenza virus vaccine candidate.
    Article Snippet: .. SeeBlue Plus2 Prestained Protein Standard marker (Thermo Fisher Scientific) was used as molecular weight marker for SDS-PAGEs and Western blots. ..

    Article Title: KRAS inhibition reverses chemotherapy resistance promoted by therapy-induced senescence-like in pancreatic ductal adenocarcinoma
    Article Snippet: .. The estimation of bands was performed using a prestained protein ladder (SeeBlue Plus2, Thermo Fisher Scientific) as a molecular weight marker. ..

    other:

    Article Title: ARNTL-mediated INO80-DHX15 axis reprograms the glycolytic metabolism and augments the progression of endometrial carcinoma
    Article Snippet: After that, 20 μg biotin-labeled cRNA was added to the cRNA fragmentation buffer and heated at 94 °C for 35 min, followed by hybridization with the GeneChip Human Genome U133A 2.0 Array (900471, Thermo Fisher Scientific) at 45 °C for 1 h. The human genome expression data were obtained by the GeneChip Scanner 3000 7 G Whole-Genome Association System (00-0362, Thermo Fisher Scientific).

    Article Title: Hypoxia activates SREBP2 through Golgi disassembly in bone marrow-derived monocytes for enhanced tumor growth
    Article Snippet: RNeasy microKit Qiagen Cat# 74134 TruSeq RNA Library Prep Illumina Cat# RS-122-2001 or RS-122-2002 Genechip Human Genome U133 plus 2.0 oligonucleotide arrays Thermo Fisher Scientific Cat# 900466 THUNDERBIRD® Probe qPCR Mix TOYOBO Cat# QPS-101 Total Cholesterol Assay Kit (Fluorometric) Cell Biolabs Cat# STA-390 QIA quick PCR purification kit QIAGEN Cat# 28181 2023 The Authors The EMBO Journal e114032 | 2023 15 of 22 Reagents and Tools table (continued) Reagent/Resource Reference or source Identifier or catalog number Duolink® Proximity Ligation Assay Kit Sigma-Aldrich Cat# DUO92008 HypoxyprobeTM-1 Plus Kit Hypoxyprobe, Inc. Cat# HP2-100 6.5 mm Transwell® with 8.0 lm Pore Polycarbonate Membrane Insert Corning Cat# 3464

    Article Title: Protein Corona Fingerprinting of Exocytosed Nanoparticles Reveals Time-Dependence of Exocytosis Pathways.
    Article Snippet: The samples were then loaded onto a 10% Bis-Tris protein gel (Thermo Fisher Scientific, Germany), with the addition of SeeBlue Plus2 Pre-Stained (Invitrogen, USA) as a marker.

    Article Title: ARNTL-mediated INO80-DHX15 axis reprograms the glycolytic metabolism and augments the progression of endometrial carcinoma.
    Article Snippet: After that, 20 μg biotin-labeled cRNA was added to the cRNA fragmentation buffer and heated at 94 °C for 35min, followed by hybridization with the GeneChip Human Genome U133A 2.0 Array (900471, Thermo Fisher Scientific) at 45 °C for 1 h. The human genome expression data were obtained by the GeneChip Scanner 3000 7 G WholeGenome Association System (00-0362, Thermo Fisher Scientific).

    Bicinchoninic Acid Protein Assay:

    Article Title: A comprehensive method of isolating proteins from serum, cerebrospinal fluid, and hippocampal neurons in rats for proteomic profiling using the LC-MS/MS platform
    Article Snippet: .. 1.50 ± 0.10 (Mucaps, India), 1.5 mL Eppendorf tubes (Tarsons, India), cotton, general anesthesia-Ketamine and xylazine, Ethanol (Supelco Merck, Germany), pre-sterilized stainless forceps, scissor, and razor (Z225622, Merck, Germany), PMSF Inhibitor Cocktail (G6521, Promega, United States), Trypsin gold, MS grade (Promega, United States), Corning tubes (15 mL, CLS430052, Merck, Germany), BCA Protein Assay kit (Thermo Scientific, USA), Methanol hypergrade for LCMS (1.06035.1000, Merck), Protein ladder (LC5925, Thermofisher Scientific, USA), Peptide Desalting Spin Columns (89852, Pierce, Sweden), Formic Acid LC-MS grade (A11750, Fisher Scientific), Water, LC-MS Grade (85189, ThermoScientific, USA), SYPRO Ruby protein stain (Invitrogen), Peptide desalting spin columns (Pierce, Thermo Scientific, USA), and IPG Buffer, pH 3–10 NL (Amersham Biosciences, USA). .. 1 mL syringe, 27 Gauge needle, Papain (76216), 9-inch-pasteurpipette (S6268), OptiPrep density gradient medium (D1556), Sucrose (S8501), Urea (U4883), Thiourea (T8656), CHAPS (850500P), Ammonium bicarbonate (A6141), Dithiothreitol (10197777001), Iodoacetamide (I1149), Acetone (650501), Acetonitrile (34851), Trifluoric acid (T62200), Magnesium choride (208337), Tris Base (252859), Triton-X100 (X100), HEPES (H3375), Ethylenediaminetetraacetic acid (EDS), Coomassie Brilliant blue G 250 (1.15444), Bromophenol Blue (114391), Glycerol (G5516), Sodium dodecyl sulfate (436143), and Agarose (A6013), were purchased from Sigma Aldrich, America).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: A comprehensive method of isolating proteins from serum, cerebrospinal fluid, and hippocampal neurons in rats for proteomic profiling using the LC-MS/MS platform
    Article Snippet: .. 1.50 ± 0.10 (Mucaps, India), 1.5 mL Eppendorf tubes (Tarsons, India), cotton, general anesthesia-Ketamine and xylazine, Ethanol (Supelco Merck, Germany), pre-sterilized stainless forceps, scissor, and razor (Z225622, Merck, Germany), PMSF Inhibitor Cocktail (G6521, Promega, United States), Trypsin gold, MS grade (Promega, United States), Corning tubes (15 mL, CLS430052, Merck, Germany), BCA Protein Assay kit (Thermo Scientific, USA), Methanol hypergrade for LCMS (1.06035.1000, Merck), Protein ladder (LC5925, Thermofisher Scientific, USA), Peptide Desalting Spin Columns (89852, Pierce, Sweden), Formic Acid LC-MS grade (A11750, Fisher Scientific), Water, LC-MS Grade (85189, ThermoScientific, USA), SYPRO Ruby protein stain (Invitrogen), Peptide desalting spin columns (Pierce, Thermo Scientific, USA), and IPG Buffer, pH 3–10 NL (Amersham Biosciences, USA). .. 1 mL syringe, 27 Gauge needle, Papain (76216), 9-inch-pasteurpipette (S6268), OptiPrep density gradient medium (D1556), Sucrose (S8501), Urea (U4883), Thiourea (T8656), CHAPS (850500P), Ammonium bicarbonate (A6141), Dithiothreitol (10197777001), Iodoacetamide (I1149), Acetone (650501), Acetonitrile (34851), Trifluoric acid (T62200), Magnesium choride (208337), Tris Base (252859), Triton-X100 (X100), HEPES (H3375), Ethylenediaminetetraacetic acid (EDS), Coomassie Brilliant blue G 250 (1.15444), Bromophenol Blue (114391), Glycerol (G5516), Sodium dodecyl sulfate (436143), and Agarose (A6013), were purchased from Sigma Aldrich, America).

    Staining:

    Article Title: A comprehensive method of isolating proteins from serum, cerebrospinal fluid, and hippocampal neurons in rats for proteomic profiling using the LC-MS/MS platform
    Article Snippet: .. 1.50 ± 0.10 (Mucaps, India), 1.5 mL Eppendorf tubes (Tarsons, India), cotton, general anesthesia-Ketamine and xylazine, Ethanol (Supelco Merck, Germany), pre-sterilized stainless forceps, scissor, and razor (Z225622, Merck, Germany), PMSF Inhibitor Cocktail (G6521, Promega, United States), Trypsin gold, MS grade (Promega, United States), Corning tubes (15 mL, CLS430052, Merck, Germany), BCA Protein Assay kit (Thermo Scientific, USA), Methanol hypergrade for LCMS (1.06035.1000, Merck), Protein ladder (LC5925, Thermofisher Scientific, USA), Peptide Desalting Spin Columns (89852, Pierce, Sweden), Formic Acid LC-MS grade (A11750, Fisher Scientific), Water, LC-MS Grade (85189, ThermoScientific, USA), SYPRO Ruby protein stain (Invitrogen), Peptide desalting spin columns (Pierce, Thermo Scientific, USA), and IPG Buffer, pH 3–10 NL (Amersham Biosciences, USA). .. 1 mL syringe, 27 Gauge needle, Papain (76216), 9-inch-pasteurpipette (S6268), OptiPrep density gradient medium (D1556), Sucrose (S8501), Urea (U4883), Thiourea (T8656), CHAPS (850500P), Ammonium bicarbonate (A6141), Dithiothreitol (10197777001), Iodoacetamide (I1149), Acetone (650501), Acetonitrile (34851), Trifluoric acid (T62200), Magnesium choride (208337), Tris Base (252859), Triton-X100 (X100), HEPES (H3375), Ethylenediaminetetraacetic acid (EDS), Coomassie Brilliant blue G 250 (1.15444), Bromophenol Blue (114391), Glycerol (G5516), Sodium dodecyl sulfate (436143), and Agarose (A6013), were purchased from Sigma Aldrich, America).

    Western Blot:

    Article Title: Purification and characterization of recombinant neuraminidase as a potentially broadly protective influenza virus vaccine candidate.
    Article Snippet: .. SeeBlue Plus2 Prestained Protein Standard marker (Thermo Fisher Scientific) was used as molecular weight marker for SDS-PAGEs and Western blots. ..



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