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cdna microarray experiments  (SuperArray Bioscience Corporation)

 
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    SuperArray Bioscience Corporation cdna microarray experiments
    Cdna Microarray Experiments, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+microarray+experiments/cdna+array/pm17139681-57-7-4
    Average 90 stars, based on 1 article reviews
    cdna microarray experiments - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Comparison:

    Article Title: Molecular and cytological features of the mouse B-cell lymphoma line iMyc Eμ -1
    Article Snippet: .. Upon comparison to normal B cells using eight different Superarray © cDNA macroarrays, the iMyc Eμ -1 cells showed the highest number of changes on the NFκB array. ..

    Microarray:

    Article Title: SMAD-Independent Down-Regulation of Caveolin-1 by TGF-β: Effects on Proliferation and Survival of Myofibroblasts
    Article Snippet: .. Superarray cDNA microarray analyses and real-time quantitative RT-PCR: RNA was isolated from cells using TRIzol reagent from Invitrogen according to the manufacturer’s protocol. .. Human extracellular matrix & adhesion molecules cDNA arrays from Superarray were used for the analyses of gene expression profiling in cells treated with/without TGF-β1 (2 ng/ml) for 24 h, according to manufacturer’s protocol (Superarray, Fredrick, MD).

    Quantitative RT-PCR:

    Article Title: SMAD-Independent Down-Regulation of Caveolin-1 by TGF-β: Effects on Proliferation and Survival of Myofibroblasts
    Article Snippet: .. Superarray cDNA microarray analyses and real-time quantitative RT-PCR: RNA was isolated from cells using TRIzol reagent from Invitrogen according to the manufacturer’s protocol. .. Human extracellular matrix & adhesion molecules cDNA arrays from Superarray were used for the analyses of gene expression profiling in cells treated with/without TGF-β1 (2 ng/ml) for 24 h, according to manufacturer’s protocol (Superarray, Fredrick, MD).

    Isolation:

    Article Title: SMAD-Independent Down-Regulation of Caveolin-1 by TGF-β: Effects on Proliferation and Survival of Myofibroblasts
    Article Snippet: .. Superarray cDNA microarray analyses and real-time quantitative RT-PCR: RNA was isolated from cells using TRIzol reagent from Invitrogen according to the manufacturer’s protocol. .. Human extracellular matrix & adhesion molecules cDNA arrays from Superarray were used for the analyses of gene expression profiling in cells treated with/without TGF-β1 (2 ng/ml) for 24 h, according to manufacturer’s protocol (Superarray, Fredrick, MD).

    Amplification:

    Article Title: Angiogenic Gene Profiles in Laser-Microdissected Microvessels and Neurons from Ischemic Penumbra of Rat Brain.
    Article Snippet: Angiogenesis is induced immediately after cerebral ischemia and plays a pivotal role in the strategy against ischemic injury.. We hypothesized that the coordinated interaction between microvessels and neurons was altered immediately after stroke, and microvessels and neurons would show the temporal specificity of angiogenic gene profiles after cerebral ischemia.. Microvessels and neurons were harvested in the ischemic penumbra of rat brain using the PixCell II laser capture microdissection (LCM) instrument.

    Expressing:

    Article Title: CDDO-Imidazolide inhibits growth and survival of c-Myc-induced mouse B cell and plasma cell neoplasms
    Article Snippet: .. The expression of 384 "pathway" genes was assessed with the help of Superarray © cDNA macroarrays and verified, in part, by RT-PCR. ..

    Article Title: Molecular and cytological features of the mouse B-cell lymphoma line iMyc Eμ -1
    Article Snippet: .. The global gene expression program of the iMyc Eμ -1 cells and the expression of 768 "pathway" genes were determined with the help of the Mouse Lymphochip © and Superarray © cDNA micro- and macroarrays, respectively. ..

    Gene Expression:

    Article Title: Molecular and cytological features of the mouse B-cell lymphoma line iMyc Eμ -1
    Article Snippet: .. The global gene expression program of the iMyc Eμ -1 cells and the expression of 768 "pathway" genes were determined with the help of the Mouse Lymphochip © and Superarray © cDNA micro- and macroarrays, respectively. ..

    Reverse Transcription:

    Article Title: Temporal profile of angiogenesis and expression of extracellular matrix-related genes in rat brains following experimental intracerebral hemorrhage.
    Article Snippet: .. A total of 3 ug RNA was reverse transcribed with biotin-16-dUTP (1093070; Roche, Germany), M-MLV reverse transcriptase (M1701; Promega, USA), and a primer mix from the cDNA array kit (MM-009N; SuperArray, USA) at 42°C for 90 min according to the manufacturer’s protocol. ..

    Article Title: Temporal profile of angiogenesis and expression of extracellular matrix-related genes in rat brains following experimental intracerebral hemorrhage
    Article Snippet: .. A total of 3 ug RNA was reverse transcribed with biotin-16-dUTP (1093070; Roche, Germany), M-MLV reverse transcriptase (M1701; Promega, USA), and a primer mix from the cDNA array kit (MM-009N; SuperArray, USA) at 42°C for 90 min according to the manufacturer's protocol. ..

    Membrane:

    Article Title: Temporal profile of angiogenesis and expression of extracellular matrix-related genes in rat brains following experimental intracerebral hemorrhage.
    Article Snippet: .. Briefly, ECM-related genes and housekeeping genes prehybridized with salmon sperm DNA (15632–011; Invitrogen) were immobilized on a nylon membrane (MM-009N; SuperArray) and hybridized with the prepared cDNA solution. ..



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    The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
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    The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
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    The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
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    Image Search Results


    The RNA samples prepared for Affymetrix microarray experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.

    Journal: Frontiers in Endocrinology

    Article Title: Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence

    doi: 10.3389/fendo.2015.00069

    Figure Lengend Snippet: The RNA samples prepared for Affymetrix microarray experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.

    Article Snippet: Totally, the detection results indicated that the quality of RNA samples we prepared ultimately meets the requirements of Affymetrix cDNA microarray experiment.

    Techniques: Microarray, Western Blot, Expressing, Transfection, Electrophoresis

    Agreement between microarray and real-time quantitative RT-PCR data . The blue block represent microarray data, the red block represent real-time PCR results. The results are mean ± SEM and the P -values are all <0.05.

    Journal: Frontiers in Endocrinology

    Article Title: Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence

    doi: 10.3389/fendo.2015.00069

    Figure Lengend Snippet: Agreement between microarray and real-time quantitative RT-PCR data . The blue block represent microarray data, the red block represent real-time PCR results. The results are mean ± SEM and the P -values are all <0.05.

    Article Snippet: Totally, the detection results indicated that the quality of RNA samples we prepared ultimately meets the requirements of Affymetrix cDNA microarray experiment.

    Techniques: Microarray, Quantitative RT-PCR, Blocking Assay, Real-time Polymerase Chain Reaction

    Verification of microarray data by quantitative real-time PCR .

    Journal: Frontiers in Endocrinology

    Article Title: Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence

    doi: 10.3389/fendo.2015.00069

    Figure Lengend Snippet: Verification of microarray data by quantitative real-time PCR .

    Article Snippet: Totally, the detection results indicated that the quality of RNA samples we prepared ultimately meets the requirements of Affymetrix cDNA microarray experiment.

    Techniques: Microarray, Real-time Polymerase Chain Reaction, Binding Assay, Activation Assay