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pbmcs  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec pbmcs
    Pbmcs, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 485 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4+kit/CD4%2B+T+Cell+Isolation+Kit%2C+human/pm42477366-435-17-20
    Average 97 stars, based on 485 article reviews
    pbmcs - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: Accurate, sensitive, and efficient chromatin accessibility quantification at target loci using UNIChro-seq.
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated with Lymphoprep density gradient medium (STEMCELL, ST07801) and stored at -150°C in CryoStor CS10 (Sigma, C2874-100ML). .. Human primary CD3+ T cells, CD4+ T cells, and B cells were isolated from freshly thawed frozen PBMCs using MACS (Myltenyi, 130-096-535, 130-096-533 and 130-101-638, respectively). .. We sorted PBMCs into eight CD4+ T cell subsets with purity > 95% using a FACS Aria III (BD Biosciences) with the flowcytometry staining panel reported in the ImmuNexUT12 (Supplementary Figure 12 and Supplementary Data 18).

    Article Title: IL-6-induced C/EBPα drives follicular regulatory T cell differentiation to regulate humoral immunity in mice.
    Article Snippet: The blood samples were diluted 2:1 in cold phosphate-buffered saline (PBS), and peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation utilizing Lymphoprep (07861, Stemcell). .. CD4 T cells were then isolated from the PBMCs using a human CD4 T cell isolation kit (130-096-533, Miltenyi Biotec), following the manufacturer’s instructions. .. CD4 CD127CD25 Treg cells were then isolated from the PBMCs using a human CD4 CD127CD25 regulatory T cell isolation kit (100-1136, STEMCELL), following the manufacturer’s instructions.

    Article Title: Dietary intervention through bacterial-derived butyrate elicits anti-tumor activity and increases anti-PD-1 response
    Article Snippet: .. Human CD4 + and CD8 + T cells were isolated from healthy subject PBMC by negative selection using magnetic beads following the manufacturer's instructions (magnetic-activated cell sorting (MACS), Miltenyi Cat# 130-096-533 and Cat# 130-096-495). ..

    Article Title: Targeting rapidly cycling receptors CD2 and CD7 increases nanoparticle delivery to primary CD4 + T cells.
    Article Snippet: .. CD4+ T cells were subsequently isolated through negative magnetic-activated cell sorting using a CD4+ T cell isolation kit (130-096-533, Miltenyi Biotec). ..

    Magnetic Cell Separation:

    Article Title: Accurate, sensitive, and efficient chromatin accessibility quantification at target loci using UNIChro-seq.
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated with Lymphoprep density gradient medium (STEMCELL, ST07801) and stored at -150°C in CryoStor CS10 (Sigma, C2874-100ML). .. Human primary CD3+ T cells, CD4+ T cells, and B cells were isolated from freshly thawed frozen PBMCs using MACS (Myltenyi, 130-096-535, 130-096-533 and 130-101-638, respectively). .. We sorted PBMCs into eight CD4+ T cell subsets with purity > 95% using a FACS Aria III (BD Biosciences) with the flowcytometry staining panel reported in the ImmuNexUT12 (Supplementary Figure 12 and Supplementary Data 18).

    Article Title: Dietary intervention through bacterial-derived butyrate elicits anti-tumor activity and increases anti-PD-1 response
    Article Snippet: .. Human CD4 + and CD8 + T cells were isolated from healthy subject PBMC by negative selection using magnetic beads following the manufacturer's instructions (magnetic-activated cell sorting (MACS), Miltenyi Cat# 130-096-533 and Cat# 130-096-495). ..

    Cell Isolation:

    Article Title: IL-6-induced C/EBPα drives follicular regulatory T cell differentiation to regulate humoral immunity in mice.
    Article Snippet: The blood samples were diluted 2:1 in cold phosphate-buffered saline (PBS), and peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation utilizing Lymphoprep (07861, Stemcell). .. CD4 T cells were then isolated from the PBMCs using a human CD4 T cell isolation kit (130-096-533, Miltenyi Biotec), following the manufacturer’s instructions. .. CD4 CD127CD25 Treg cells were then isolated from the PBMCs using a human CD4 CD127CD25 regulatory T cell isolation kit (100-1136, STEMCELL), following the manufacturer’s instructions.

    Article Title: Targeting rapidly cycling receptors CD2 and CD7 increases nanoparticle delivery to primary CD4 + T cells.
    Article Snippet: .. CD4+ T cells were subsequently isolated through negative magnetic-activated cell sorting using a CD4+ T cell isolation kit (130-096-533, Miltenyi Biotec). ..

    Selection:

    Article Title: Dietary intervention through bacterial-derived butyrate elicits anti-tumor activity and increases anti-PD-1 response
    Article Snippet: .. Human CD4 + and CD8 + T cells were isolated from healthy subject PBMC by negative selection using magnetic beads following the manufacturer's instructions (magnetic-activated cell sorting (MACS), Miltenyi Cat# 130-096-533 and Cat# 130-096-495). ..

    Magnetic Beads:

    Article Title: Dietary intervention through bacterial-derived butyrate elicits anti-tumor activity and increases anti-PD-1 response
    Article Snippet: .. Human CD4 + and CD8 + T cells were isolated from healthy subject PBMC by negative selection using magnetic beads following the manufacturer's instructions (magnetic-activated cell sorting (MACS), Miltenyi Cat# 130-096-533 and Cat# 130-096-495). ..

    FACS:

    Article Title: Dietary intervention through bacterial-derived butyrate elicits anti-tumor activity and increases anti-PD-1 response
    Article Snippet: .. Human CD4 + and CD8 + T cells were isolated from healthy subject PBMC by negative selection using magnetic beads following the manufacturer's instructions (magnetic-activated cell sorting (MACS), Miltenyi Cat# 130-096-533 and Cat# 130-096-495). ..

    Article Title: Targeting rapidly cycling receptors CD2 and CD7 increases nanoparticle delivery to primary CD4 + T cells.
    Article Snippet: .. CD4+ T cells were subsequently isolated through negative magnetic-activated cell sorting using a CD4+ T cell isolation kit (130-096-533, Miltenyi Biotec). ..



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    Image Search Results


    TRPM2 deficiency is associated with attenuated Ca 2+ ‐NFAT signaling during T cell activation. (A) Intracellular Ca 2+ flux in splenic CD4 + T cells from WT and TRPM2 −/− mice, measured by flow cytometry using Fluo‐4 AM after stimulation with anti‐CD3/CD28 antibodies (5 μg/mL each). The graph shows the mean fluorescence intensity (MFI) over time from one representative experiment of three independent replicates. (B) Nuclear translocation of NFATc1 and IL‐2 production were assessed by Western blot from WT and TRPM2 −/− mice.

    Journal: Immunity, Inflammation and Disease

    Article Title: TRPM2 Deficiency Attenuates Allergic Rhinitis‐Like Inflammation With Altered Ca 2+ ‐NFAT Signaling, Treg Responses, and sIgE Production

    doi: 10.1002/iid3.70490

    Figure Lengend Snippet: TRPM2 deficiency is associated with attenuated Ca 2+ ‐NFAT signaling during T cell activation. (A) Intracellular Ca 2+ flux in splenic CD4 + T cells from WT and TRPM2 −/− mice, measured by flow cytometry using Fluo‐4 AM after stimulation with anti‐CD3/CD28 antibodies (5 μg/mL each). The graph shows the mean fluorescence intensity (MFI) over time from one representative experiment of three independent replicates. (B) Nuclear translocation of NFATc1 and IL‐2 production were assessed by Western blot from WT and TRPM2 −/− mice.

    Article Snippet: Naïve CD4 + T cells were then isolated by negative selection using a commercial mouse CD4 + T Cell Isolation Kit (Cat. No. 130‐104‐454, Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer's instructions.

    Techniques: Activation Assay, Flow Cytometry, Fluorescence, Translocation Assay, Western Blot