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fc block buffer  (Bio-Rad)


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    Bio-Rad fc block buffer
    Fc Block Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd32/Rat+anti+Mouse+CD16%2FCD32/pm41663544-252-16-18
    Average 93 stars, based on 35 article reviews
    fc block buffer - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: A novel real time imaging platform to quantify macrophage phagocytosis.
    Article Snippet: Red blood cells were lysed with BD Pharm LyseTM (BD Biosciences, Oxford, UK) as instructed by the manufacturer and were re-suspended in 0.5 ml PBS/1% FBS for further use. .. Lung and peritoneal harvested cells were blocked with 10 lg/ml rat anti-mouse CD16/CD32 (AbD Serotec, Oxford, UK) and 1.17 mg/ ml murine immunoglobulin G (IgG) (Jackson ImmunoResearch Laboratories, PA, USA) in Flow Cytometry buffer (PBS; 2% FBS, 25 mM HEPES, 5 mM EDTA) for 15 min on ice. .. Cells were stained with anti-CD45 (30-F11; BD Pharmigen), anti-CD11c (HL3; BD Pharmigen), anti-Siglec F (E50-2440; BD Pharmigen), anti-CD11b (M1/70; Biolegend, London, UK) and anti-F4/80 (CI:A3-1; Bio-rad) at 2 lg/ml in Flow Cytometry buffer for 30 min on ice protected from light.

    Article Title: A novel real time imaging platform to quantify macrophage phagocytosis
    Article Snippet: Red blood cells were lysed with BD Pharm LyseTM (BD Biosciences, Oxford, UK) as instructed by the manufacturer and were re-suspended in 0.5 ml PBS/1% FBS for further use. .. Lung and peritoneal harvested cells were blocked with 10 μg/ml rat anti-mouse CD16/CD32 (AbD Serotec, Oxford, UK) and 1.17 mg/ml murine immunoglobulin G (IgG) (Jackson ImmunoResearch Laboratories, PA, USA) in Flow Cytometry buffer (PBS; 2% FBS, 25 mM HEPES, 5 mM EDTA) for 15 min on ice. .. Cells were stained with anti-CD45 (30-F11; BD Pharmigen), anti-CD11c (HL3; BD Pharmigen), anti-Siglec F (E50-2440; BD Pharmigen), anti-CD11b (M1/70; Biolegend, London, UK) and anti-F4/80 (CI:A3-1; Bio-rad) at 2 μg/ml in Flow Cytometry buffer for 30 min on ice protected from light.

    Incubation:

    Article Title: A broadly adaptable protocol for isolating Kupffer cells from non-model species: application to Mastomys natalensis and its susceptibility to Old World mammarenaviruses
    Article Snippet: .. For flow cytometric analysis, the cells were harvested and first incubated with Fc-block buffer (BioRad #MCA2305) for 15 mins. .. After that, the cells were incubated with antibodies against CD11b (Biolegend #101216, 1:200), MHC-II (Invitrogen #12-5980-82, 1:200), CD80 (Biolegend #104713, 1:200), Iba1 (Fujifilm #019-19741, 1:50) and ZombieNIR (Biolegend #423106, 1:200) for 15 min at room temperature in the dark.

    Article Title: Lassa virus circumvents macrophage and dendritic cell antiviral defences in its natural reservoir, the Natal multimammate mouse (Mastomys natalensis).
    Article Snippet: .. For flow cytometric analysis, the cells were harvested at the corresponding timepoints and first incubated with Fc-block buffer (BioRad #MCA2305) for 15min. .. In parallel, the cells were stimulated with8 μg/mLofLPS (InvivoGen), 25 ng/mLofPoly I:C (HMW, InvivoGen) or 30 HAU of SeV, for the corresponding timepoints.

    other:

    Article Title: Lassa virus circumvents macrophage and dendritic cell antiviral defences in its natural reservoir, the Natal multimammate mouse ( Mastomys natalensis )
    Article Snippet: Lastly, the samples were stained using the TBM buffer (RecomBlot, Mikrogen) following the manufacturer’s instructions.

    Control:

    Article Title: CD13/aminopeptidase N is a negative regulator of mast cell activation.
    Article Snippet: Antigen-inducedmast cell (MC) activation via cross-linking of IgE-bound high-affinity receptors for IgE (Fc«RI) underlies type I allergy and anaphylactic shock.. Comprehensive knowledge of Fc«RI regulation is thus required.. We have identified a functional interaction between Fc«RI and CD13 in murineMCs.

    Purification:

    Article Title: Protocol for enrichment, purification, and cytocentrifugation of mouse liver endothelial cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Allophycocyanin/cyanine 7 anti-mouse CD45 (1:100) BioLegend Cat#103116 BV605 anti-mouse CD31 (1:100) BioLegend Cat#102427 (Continued on next page) 2 STAR Protocols 4, 102480, September 15, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Purified Rat Anti-Mouse CD16/CD32 Clone 2.4G2 (RUO) (1:100) BD Pharmingen Cat #553142 Chemicals, peptides, and recombinant proteins 103 Tris buffered saline (TBS) Bio-Rad Cat#1706463 16% Paraformaldehyde (formaldehyde) aqueous solution, EM grade Thomas Scientific Cat# C993M26 Ammonium chloride (NH4Cl), crystal VWR Chemicals Cat#12125-02-9 Fetal bovine serum (FBS) Gibco Cat#10437-028 DAPI (1:100) BioLegend Cat#422801 PBS (103), pH 7.4 Thermo Fisher Scientific Cat#70011069 PBS (13), pH 7.4 Thermo Fisher Scientific Cat#10010023 Percoll Sigma-Aldrich Cat#17-0891-02 Prolong Gold antifade reagent with DAPI Invitrogen Cat#P36980 RPMI 1640 Medium Thermo Fisher Scientific Cat#11875093 Sodium azide, 5% Fisher Scientific Cat#71448-16 Triton X-100 American Bio Cat#9002-93-1 UltraPure 0.5 M EDTA, pH 8.0 Life Technologies Cat#15575020 Other Cover Glass Thickness 1 1/2 22 3 22 mm Corning Cat#2850-22 Epredia Single Cytofunnel with White Filter Cards Thermo Fisher Scientific Cat#5991040 Epredia Cytoclip Stainless-Steel Slide Clip Thermo Fisher Scientific Cat#59910052 Glass Coplin Jar Thermo Fisher Scientific Cat#E94 Unifrost Plus Microscope Slides Adhesive, Treated Surfaces Azer Scientific Cat#EMS200G+ ImmEdge Hydrophobic Barrier PAP Pen Vector Laboratories Cat#H-4000 Thermo Scientific Cytospin 4 Cytocentrifuge Thermo Fisher Scientific Cat# A78300003 Sorvall Legend T Benchtop Centrifuge Rotor: 75006445 Sorvall Legend Cat#SO-LEGT BD FACS Aria II sorter BD Biosciences N/A Beckman Coulter Allegra 6 Centrifuge Rotor: GH-3.8 Swing Bucket Rotor Beckman Coulter Cat#BE-A6 Leica TCS SP8 Leica N/A Software and algorithms FlowJo 10 BD http://flowjo.com ll OPEN ACCESSProtocol ..

    Recombinant:

    Article Title: Protocol for enrichment, purification, and cytocentrifugation of mouse liver endothelial cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Allophycocyanin/cyanine 7 anti-mouse CD45 (1:100) BioLegend Cat#103116 BV605 anti-mouse CD31 (1:100) BioLegend Cat#102427 (Continued on next page) 2 STAR Protocols 4, 102480, September 15, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Purified Rat Anti-Mouse CD16/CD32 Clone 2.4G2 (RUO) (1:100) BD Pharmingen Cat #553142 Chemicals, peptides, and recombinant proteins 103 Tris buffered saline (TBS) Bio-Rad Cat#1706463 16% Paraformaldehyde (formaldehyde) aqueous solution, EM grade Thomas Scientific Cat# C993M26 Ammonium chloride (NH4Cl), crystal VWR Chemicals Cat#12125-02-9 Fetal bovine serum (FBS) Gibco Cat#10437-028 DAPI (1:100) BioLegend Cat#422801 PBS (103), pH 7.4 Thermo Fisher Scientific Cat#70011069 PBS (13), pH 7.4 Thermo Fisher Scientific Cat#10010023 Percoll Sigma-Aldrich Cat#17-0891-02 Prolong Gold antifade reagent with DAPI Invitrogen Cat#P36980 RPMI 1640 Medium Thermo Fisher Scientific Cat#11875093 Sodium azide, 5% Fisher Scientific Cat#71448-16 Triton X-100 American Bio Cat#9002-93-1 UltraPure 0.5 M EDTA, pH 8.0 Life Technologies Cat#15575020 Other Cover Glass Thickness 1 1/2 22 3 22 mm Corning Cat#2850-22 Epredia Single Cytofunnel with White Filter Cards Thermo Fisher Scientific Cat#5991040 Epredia Cytoclip Stainless-Steel Slide Clip Thermo Fisher Scientific Cat#59910052 Glass Coplin Jar Thermo Fisher Scientific Cat#E94 Unifrost Plus Microscope Slides Adhesive, Treated Surfaces Azer Scientific Cat#EMS200G+ ImmEdge Hydrophobic Barrier PAP Pen Vector Laboratories Cat#H-4000 Thermo Scientific Cytospin 4 Cytocentrifuge Thermo Fisher Scientific Cat# A78300003 Sorvall Legend T Benchtop Centrifuge Rotor: 75006445 Sorvall Legend Cat#SO-LEGT BD FACS Aria II sorter BD Biosciences N/A Beckman Coulter Allegra 6 Centrifuge Rotor: GH-3.8 Swing Bucket Rotor Beckman Coulter Cat#BE-A6 Leica TCS SP8 Leica N/A Software and algorithms FlowJo 10 BD http://flowjo.com ll OPEN ACCESSProtocol ..

    Saline:

    Article Title: Protocol for enrichment, purification, and cytocentrifugation of mouse liver endothelial cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Allophycocyanin/cyanine 7 anti-mouse CD45 (1:100) BioLegend Cat#103116 BV605 anti-mouse CD31 (1:100) BioLegend Cat#102427 (Continued on next page) 2 STAR Protocols 4, 102480, September 15, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Purified Rat Anti-Mouse CD16/CD32 Clone 2.4G2 (RUO) (1:100) BD Pharmingen Cat #553142 Chemicals, peptides, and recombinant proteins 103 Tris buffered saline (TBS) Bio-Rad Cat#1706463 16% Paraformaldehyde (formaldehyde) aqueous solution, EM grade Thomas Scientific Cat# C993M26 Ammonium chloride (NH4Cl), crystal VWR Chemicals Cat#12125-02-9 Fetal bovine serum (FBS) Gibco Cat#10437-028 DAPI (1:100) BioLegend Cat#422801 PBS (103), pH 7.4 Thermo Fisher Scientific Cat#70011069 PBS (13), pH 7.4 Thermo Fisher Scientific Cat#10010023 Percoll Sigma-Aldrich Cat#17-0891-02 Prolong Gold antifade reagent with DAPI Invitrogen Cat#P36980 RPMI 1640 Medium Thermo Fisher Scientific Cat#11875093 Sodium azide, 5% Fisher Scientific Cat#71448-16 Triton X-100 American Bio Cat#9002-93-1 UltraPure 0.5 M EDTA, pH 8.0 Life Technologies Cat#15575020 Other Cover Glass Thickness 1 1/2 22 3 22 mm Corning Cat#2850-22 Epredia Single Cytofunnel with White Filter Cards Thermo Fisher Scientific Cat#5991040 Epredia Cytoclip Stainless-Steel Slide Clip Thermo Fisher Scientific Cat#59910052 Glass Coplin Jar Thermo Fisher Scientific Cat#E94 Unifrost Plus Microscope Slides Adhesive, Treated Surfaces Azer Scientific Cat#EMS200G+ ImmEdge Hydrophobic Barrier PAP Pen Vector Laboratories Cat#H-4000 Thermo Scientific Cytospin 4 Cytocentrifuge Thermo Fisher Scientific Cat# A78300003 Sorvall Legend T Benchtop Centrifuge Rotor: 75006445 Sorvall Legend Cat#SO-LEGT BD FACS Aria II sorter BD Biosciences N/A Beckman Coulter Allegra 6 Centrifuge Rotor: GH-3.8 Swing Bucket Rotor Beckman Coulter Cat#BE-A6 Leica TCS SP8 Leica N/A Software and algorithms FlowJo 10 BD http://flowjo.com ll OPEN ACCESSProtocol ..

    Microscopy:

    Article Title: Protocol for enrichment, purification, and cytocentrifugation of mouse liver endothelial cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Allophycocyanin/cyanine 7 anti-mouse CD45 (1:100) BioLegend Cat#103116 BV605 anti-mouse CD31 (1:100) BioLegend Cat#102427 (Continued on next page) 2 STAR Protocols 4, 102480, September 15, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Purified Rat Anti-Mouse CD16/CD32 Clone 2.4G2 (RUO) (1:100) BD Pharmingen Cat #553142 Chemicals, peptides, and recombinant proteins 103 Tris buffered saline (TBS) Bio-Rad Cat#1706463 16% Paraformaldehyde (formaldehyde) aqueous solution, EM grade Thomas Scientific Cat# C993M26 Ammonium chloride (NH4Cl), crystal VWR Chemicals Cat#12125-02-9 Fetal bovine serum (FBS) Gibco Cat#10437-028 DAPI (1:100) BioLegend Cat#422801 PBS (103), pH 7.4 Thermo Fisher Scientific Cat#70011069 PBS (13), pH 7.4 Thermo Fisher Scientific Cat#10010023 Percoll Sigma-Aldrich Cat#17-0891-02 Prolong Gold antifade reagent with DAPI Invitrogen Cat#P36980 RPMI 1640 Medium Thermo Fisher Scientific Cat#11875093 Sodium azide, 5% Fisher Scientific Cat#71448-16 Triton X-100 American Bio Cat#9002-93-1 UltraPure 0.5 M EDTA, pH 8.0 Life Technologies Cat#15575020 Other Cover Glass Thickness 1 1/2 22 3 22 mm Corning Cat#2850-22 Epredia Single Cytofunnel with White Filter Cards Thermo Fisher Scientific Cat#5991040 Epredia Cytoclip Stainless-Steel Slide Clip Thermo Fisher Scientific Cat#59910052 Glass Coplin Jar Thermo Fisher Scientific Cat#E94 Unifrost Plus Microscope Slides Adhesive, Treated Surfaces Azer Scientific Cat#EMS200G+ ImmEdge Hydrophobic Barrier PAP Pen Vector Laboratories Cat#H-4000 Thermo Scientific Cytospin 4 Cytocentrifuge Thermo Fisher Scientific Cat# A78300003 Sorvall Legend T Benchtop Centrifuge Rotor: 75006445 Sorvall Legend Cat#SO-LEGT BD FACS Aria II sorter BD Biosciences N/A Beckman Coulter Allegra 6 Centrifuge Rotor: GH-3.8 Swing Bucket Rotor Beckman Coulter Cat#BE-A6 Leica TCS SP8 Leica N/A Software and algorithms FlowJo 10 BD http://flowjo.com ll OPEN ACCESSProtocol ..

    Adhesive:

    Article Title: Protocol for enrichment, purification, and cytocentrifugation of mouse liver endothelial cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Allophycocyanin/cyanine 7 anti-mouse CD45 (1:100) BioLegend Cat#103116 BV605 anti-mouse CD31 (1:100) BioLegend Cat#102427 (Continued on next page) 2 STAR Protocols 4, 102480, September 15, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Purified Rat Anti-Mouse CD16/CD32 Clone 2.4G2 (RUO) (1:100) BD Pharmingen Cat #553142 Chemicals, peptides, and recombinant proteins 103 Tris buffered saline (TBS) Bio-Rad Cat#1706463 16% Paraformaldehyde (formaldehyde) aqueous solution, EM grade Thomas Scientific Cat# C993M26 Ammonium chloride (NH4Cl), crystal VWR Chemicals Cat#12125-02-9 Fetal bovine serum (FBS) Gibco Cat#10437-028 DAPI (1:100) BioLegend Cat#422801 PBS (103), pH 7.4 Thermo Fisher Scientific Cat#70011069 PBS (13), pH 7.4 Thermo Fisher Scientific Cat#10010023 Percoll Sigma-Aldrich Cat#17-0891-02 Prolong Gold antifade reagent with DAPI Invitrogen Cat#P36980 RPMI 1640 Medium Thermo Fisher Scientific Cat#11875093 Sodium azide, 5% Fisher Scientific Cat#71448-16 Triton X-100 American Bio Cat#9002-93-1 UltraPure 0.5 M EDTA, pH 8.0 Life Technologies Cat#15575020 Other Cover Glass Thickness 1 1/2 22 3 22 mm Corning Cat#2850-22 Epredia Single Cytofunnel with White Filter Cards Thermo Fisher Scientific Cat#5991040 Epredia Cytoclip Stainless-Steel Slide Clip Thermo Fisher Scientific Cat#59910052 Glass Coplin Jar Thermo Fisher Scientific Cat#E94 Unifrost Plus Microscope Slides Adhesive, Treated Surfaces Azer Scientific Cat#EMS200G+ ImmEdge Hydrophobic Barrier PAP Pen Vector Laboratories Cat#H-4000 Thermo Scientific Cytospin 4 Cytocentrifuge Thermo Fisher Scientific Cat# A78300003 Sorvall Legend T Benchtop Centrifuge Rotor: 75006445 Sorvall Legend Cat#SO-LEGT BD FACS Aria II sorter BD Biosciences N/A Beckman Coulter Allegra 6 Centrifuge Rotor: GH-3.8 Swing Bucket Rotor Beckman Coulter Cat#BE-A6 Leica TCS SP8 Leica N/A Software and algorithms FlowJo 10 BD http://flowjo.com ll OPEN ACCESSProtocol ..

    FACS:

    Article Title: Protocol for enrichment, purification, and cytocentrifugation of mouse liver endothelial cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Allophycocyanin/cyanine 7 anti-mouse CD45 (1:100) BioLegend Cat#103116 BV605 anti-mouse CD31 (1:100) BioLegend Cat#102427 (Continued on next page) 2 STAR Protocols 4, 102480, September 15, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Purified Rat Anti-Mouse CD16/CD32 Clone 2.4G2 (RUO) (1:100) BD Pharmingen Cat #553142 Chemicals, peptides, and recombinant proteins 103 Tris buffered saline (TBS) Bio-Rad Cat#1706463 16% Paraformaldehyde (formaldehyde) aqueous solution, EM grade Thomas Scientific Cat# C993M26 Ammonium chloride (NH4Cl), crystal VWR Chemicals Cat#12125-02-9 Fetal bovine serum (FBS) Gibco Cat#10437-028 DAPI (1:100) BioLegend Cat#422801 PBS (103), pH 7.4 Thermo Fisher Scientific Cat#70011069 PBS (13), pH 7.4 Thermo Fisher Scientific Cat#10010023 Percoll Sigma-Aldrich Cat#17-0891-02 Prolong Gold antifade reagent with DAPI Invitrogen Cat#P36980 RPMI 1640 Medium Thermo Fisher Scientific Cat#11875093 Sodium azide, 5% Fisher Scientific Cat#71448-16 Triton X-100 American Bio Cat#9002-93-1 UltraPure 0.5 M EDTA, pH 8.0 Life Technologies Cat#15575020 Other Cover Glass Thickness 1 1/2 22 3 22 mm Corning Cat#2850-22 Epredia Single Cytofunnel with White Filter Cards Thermo Fisher Scientific Cat#5991040 Epredia Cytoclip Stainless-Steel Slide Clip Thermo Fisher Scientific Cat#59910052 Glass Coplin Jar Thermo Fisher Scientific Cat#E94 Unifrost Plus Microscope Slides Adhesive, Treated Surfaces Azer Scientific Cat#EMS200G+ ImmEdge Hydrophobic Barrier PAP Pen Vector Laboratories Cat#H-4000 Thermo Scientific Cytospin 4 Cytocentrifuge Thermo Fisher Scientific Cat# A78300003 Sorvall Legend T Benchtop Centrifuge Rotor: 75006445 Sorvall Legend Cat#SO-LEGT BD FACS Aria II sorter BD Biosciences N/A Beckman Coulter Allegra 6 Centrifuge Rotor: GH-3.8 Swing Bucket Rotor Beckman Coulter Cat#BE-A6 Leica TCS SP8 Leica N/A Software and algorithms FlowJo 10 BD http://flowjo.com ll OPEN ACCESSProtocol ..

    Software:

    Article Title: Protocol for enrichment, purification, and cytocentrifugation of mouse liver endothelial cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Allophycocyanin/cyanine 7 anti-mouse CD45 (1:100) BioLegend Cat#103116 BV605 anti-mouse CD31 (1:100) BioLegend Cat#102427 (Continued on next page) 2 STAR Protocols 4, 102480, September 15, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Purified Rat Anti-Mouse CD16/CD32 Clone 2.4G2 (RUO) (1:100) BD Pharmingen Cat #553142 Chemicals, peptides, and recombinant proteins 103 Tris buffered saline (TBS) Bio-Rad Cat#1706463 16% Paraformaldehyde (formaldehyde) aqueous solution, EM grade Thomas Scientific Cat# C993M26 Ammonium chloride (NH4Cl), crystal VWR Chemicals Cat#12125-02-9 Fetal bovine serum (FBS) Gibco Cat#10437-028 DAPI (1:100) BioLegend Cat#422801 PBS (103), pH 7.4 Thermo Fisher Scientific Cat#70011069 PBS (13), pH 7.4 Thermo Fisher Scientific Cat#10010023 Percoll Sigma-Aldrich Cat#17-0891-02 Prolong Gold antifade reagent with DAPI Invitrogen Cat#P36980 RPMI 1640 Medium Thermo Fisher Scientific Cat#11875093 Sodium azide, 5% Fisher Scientific Cat#71448-16 Triton X-100 American Bio Cat#9002-93-1 UltraPure 0.5 M EDTA, pH 8.0 Life Technologies Cat#15575020 Other Cover Glass Thickness 1 1/2 22 3 22 mm Corning Cat#2850-22 Epredia Single Cytofunnel with White Filter Cards Thermo Fisher Scientific Cat#5991040 Epredia Cytoclip Stainless-Steel Slide Clip Thermo Fisher Scientific Cat#59910052 Glass Coplin Jar Thermo Fisher Scientific Cat#E94 Unifrost Plus Microscope Slides Adhesive, Treated Surfaces Azer Scientific Cat#EMS200G+ ImmEdge Hydrophobic Barrier PAP Pen Vector Laboratories Cat#H-4000 Thermo Scientific Cytospin 4 Cytocentrifuge Thermo Fisher Scientific Cat# A78300003 Sorvall Legend T Benchtop Centrifuge Rotor: 75006445 Sorvall Legend Cat#SO-LEGT BD FACS Aria II sorter BD Biosciences N/A Beckman Coulter Allegra 6 Centrifuge Rotor: GH-3.8 Swing Bucket Rotor Beckman Coulter Cat#BE-A6 Leica TCS SP8 Leica N/A Software and algorithms FlowJo 10 BD http://flowjo.com ll OPEN ACCESSProtocol ..



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