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ca 2+ detection kit  (Beyotime)


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    Beyotime ca 2+ detection kit
    Ca 2+ Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca+2++detection+kit/ca2++kit/pmc11937690-75-8-12
    Average 90 stars, based on 1 article reviews
    ca 2+ detection kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Membrane:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress- and Ca 2+ Imbalance-Mediated Endoplasmic Reticulum Stress.
    Article Snippet: Ca2+ kit, 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), and NAC were from Beyotime (Nanjing, China).

    Article Title: ITRAQ-based quantitative proteomic analysis of MG63 in response to HIF-1α inducers.
    Article Snippet: Non-healing fractures constitute a serious clinical problem.. HIF-1α is a crucial regulator in response to hypoxia and is proven to be pivotal in bone growth; however, the mechanism still needs further research.. In this study, iTRAQ was used to study the effects of two HIF-1α inducers on the expression of proteins in MG63 cells.

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: Anticancer activity of four trinuclear cobalt complexes bearing bis(salicylidene)-1,3-propanediamine derivatives.
    Article Snippet: Schiff base and its complexes are being paid more and more interests for their great prospects in biological applications.. We reported here four cobalt(II) complexes [Co3(L)2(HCOO)2] (1), [Co3(L)2(HCOO)2(CH3OH)2]⋅ 2CH3OH (2), [Co3(HL)2(OAc)2(DMF)2] (3) and [Co3(HL)2(HCOO)2(DMF)2]⋅2H2O (4) bearing the bis-Schiff base ligand of bis(5-bromosalicylidene)-1,3-propanediamine (H2L), bis(5-bromosalicylidene)-2-methyl-1,3propanediamine (H2L), bis(5-chlorosalicylidene)-2-hydroxyl-1,3-propanediamine (H3L) and bis(5bromosalicylidene)-2-hydroxyl-1,3-propanediamine (H3L), respectively.. The anti-tumor activities of the four titled complexes were screened on a series of tumor cell lines.

    Article Title: Arsenite increases Cyclin D1 expression through coordinated regulation of the Ca 2+ /NFAT2 and NF-κB pathways via ERK/MAPK in a human uroepithelial cell line.
    Article Snippet: To understand the direct link between Cyclin D1, and nuclear factor of activated T cells 2 (NFAT2) and nuclear factor (NF)-kB in arsenic-treated bladder cells, as well as the association between MAPK and NFAT signaling, we determined whether or not the Ca/NFAT pathway is activated in an arsenic-treated normal urothelial cell line and determined the roles of NFAT and NF-kB signals in the regulation of Cyclin D1 expression.. The SV-40 immortalized human uroepithelial cell line, SV-HUC-1, was treated with NaAsO2 for 24 h (0, 1, 2, 4, 8, and 10 mM) and 10, 20, 30, and 40 weeks (0 and 0.5 mM).. We found that arsenite increased the intracellular Ca levels and induced NFAT2 nuclear translocation after treatment for 24 h. The level of NFAT2 mRNA and expression of total protein and nuclear protein were increased after long-term treatment with 0.5 mM arsenite for 30 and 40 weeks compared to the cells treated for 24 h. In addition, NF-kB p50 and p65 nuclear protein expression increased significantly in cells treated with 2–8 mM arsenite for 24 h, which was consistent with NFAT2 nuclear expression.

    Article Title: SiO 2 particles induce pulmonary fibrosis by modulating NLRP3 through the ROS/Keap1/Nrf2 signaling pathway in rats.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Flow Cytometry:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress- and Ca 2+ Imbalance-Mediated Endoplasmic Reticulum Stress.
    Article Snippet: Ca2+ kit, 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), and NAC were from Beyotime (Nanjing, China).

    Article Title: ITRAQ-based quantitative proteomic analysis of MG63 in response to HIF-1α inducers.
    Article Snippet: Non-healing fractures constitute a serious clinical problem.. HIF-1α is a crucial regulator in response to hypoxia and is proven to be pivotal in bone growth; however, the mechanism still needs further research.. In this study, iTRAQ was used to study the effects of two HIF-1α inducers on the expression of proteins in MG63 cells.

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: Anticancer activity of four trinuclear cobalt complexes bearing bis(salicylidene)-1,3-propanediamine derivatives.
    Article Snippet: Schiff base and its complexes are being paid more and more interests for their great prospects in biological applications.. We reported here four cobalt(II) complexes [Co3(L)2(HCOO)2] (1), [Co3(L)2(HCOO)2(CH3OH)2]⋅ 2CH3OH (2), [Co3(HL)2(OAc)2(DMF)2] (3) and [Co3(HL)2(HCOO)2(DMF)2]⋅2H2O (4) bearing the bis-Schiff base ligand of bis(5-bromosalicylidene)-1,3-propanediamine (H2L), bis(5-bromosalicylidene)-2-methyl-1,3propanediamine (H2L), bis(5-chlorosalicylidene)-2-hydroxyl-1,3-propanediamine (H3L) and bis(5bromosalicylidene)-2-hydroxyl-1,3-propanediamine (H3L), respectively.. The anti-tumor activities of the four titled complexes were screened on a series of tumor cell lines.

    Article Title: Arsenite increases Cyclin D1 expression through coordinated regulation of the Ca 2+ /NFAT2 and NF-κB pathways via ERK/MAPK in a human uroepithelial cell line.
    Article Snippet: To understand the direct link between Cyclin D1, and nuclear factor of activated T cells 2 (NFAT2) and nuclear factor (NF)-kB in arsenic-treated bladder cells, as well as the association between MAPK and NFAT signaling, we determined whether or not the Ca/NFAT pathway is activated in an arsenic-treated normal urothelial cell line and determined the roles of NFAT and NF-kB signals in the regulation of Cyclin D1 expression.. The SV-40 immortalized human uroepithelial cell line, SV-HUC-1, was treated with NaAsO2 for 24 h (0, 1, 2, 4, 8, and 10 mM) and 10, 20, 30, and 40 weeks (0 and 0.5 mM).. We found that arsenite increased the intracellular Ca levels and induced NFAT2 nuclear translocation after treatment for 24 h. The level of NFAT2 mRNA and expression of total protein and nuclear protein were increased after long-term treatment with 0.5 mM arsenite for 30 and 40 weeks compared to the cells treated for 24 h. In addition, NF-kB p50 and p65 nuclear protein expression increased significantly in cells treated with 2–8 mM arsenite for 24 h, which was consistent with NFAT2 nuclear expression.

    Article Title: SiO 2 particles induce pulmonary fibrosis by modulating NLRP3 through the ROS/Keap1/Nrf2 signaling pathway in rats.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Expressing:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress- and Ca 2+ Imbalance-Mediated Endoplasmic Reticulum Stress.
    Article Snippet: Ca2+ kit, 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), and NAC were from Beyotime (Nanjing, China).

    Article Title: ITRAQ-based quantitative proteomic analysis of MG63 in response to HIF-1α inducers.
    Article Snippet: Non-healing fractures constitute a serious clinical problem.. HIF-1α is a crucial regulator in response to hypoxia and is proven to be pivotal in bone growth; however, the mechanism still needs further research.. In this study, iTRAQ was used to study the effects of two HIF-1α inducers on the expression of proteins in MG63 cells.

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: Anticancer activity of four trinuclear cobalt complexes bearing bis(salicylidene)-1,3-propanediamine derivatives.
    Article Snippet: Schiff base and its complexes are being paid more and more interests for their great prospects in biological applications.. We reported here four cobalt(II) complexes [Co3(L)2(HCOO)2] (1), [Co3(L)2(HCOO)2(CH3OH)2]⋅ 2CH3OH (2), [Co3(HL)2(OAc)2(DMF)2] (3) and [Co3(HL)2(HCOO)2(DMF)2]⋅2H2O (4) bearing the bis-Schiff base ligand of bis(5-bromosalicylidene)-1,3-propanediamine (H2L), bis(5-bromosalicylidene)-2-methyl-1,3propanediamine (H2L), bis(5-chlorosalicylidene)-2-hydroxyl-1,3-propanediamine (H3L) and bis(5bromosalicylidene)-2-hydroxyl-1,3-propanediamine (H3L), respectively.. The anti-tumor activities of the four titled complexes were screened on a series of tumor cell lines.

    Article Title: Arsenite increases Cyclin D1 expression through coordinated regulation of the Ca 2+ /NFAT2 and NF-κB pathways via ERK/MAPK in a human uroepithelial cell line.
    Article Snippet: To understand the direct link between Cyclin D1, and nuclear factor of activated T cells 2 (NFAT2) and nuclear factor (NF)-kB in arsenic-treated bladder cells, as well as the association between MAPK and NFAT signaling, we determined whether or not the Ca/NFAT pathway is activated in an arsenic-treated normal urothelial cell line and determined the roles of NFAT and NF-kB signals in the regulation of Cyclin D1 expression.. The SV-40 immortalized human uroepithelial cell line, SV-HUC-1, was treated with NaAsO2 for 24 h (0, 1, 2, 4, 8, and 10 mM) and 10, 20, 30, and 40 weeks (0 and 0.5 mM).. We found that arsenite increased the intracellular Ca levels and induced NFAT2 nuclear translocation after treatment for 24 h. The level of NFAT2 mRNA and expression of total protein and nuclear protein were increased after long-term treatment with 0.5 mM arsenite for 30 and 40 weeks compared to the cells treated for 24 h. In addition, NF-kB p50 and p65 nuclear protein expression increased significantly in cells treated with 2–8 mM arsenite for 24 h, which was consistent with NFAT2 nuclear expression.

    Article Title: SiO 2 particles induce pulmonary fibrosis by modulating NLRP3 through the ROS/Keap1/Nrf2 signaling pathway in rats.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Concentration Assay:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress- and Ca 2+ Imbalance-Mediated Endoplasmic Reticulum Stress.
    Article Snippet: Ca2+ kit, 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), and NAC were from Beyotime (Nanjing, China).

    Article Title: ITRAQ-based quantitative proteomic analysis of MG63 in response to HIF-1α inducers.
    Article Snippet: Non-healing fractures constitute a serious clinical problem.. HIF-1α is a crucial regulator in response to hypoxia and is proven to be pivotal in bone growth; however, the mechanism still needs further research.. In this study, iTRAQ was used to study the effects of two HIF-1α inducers on the expression of proteins in MG63 cells.

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: Anticancer activity of four trinuclear cobalt complexes bearing bis(salicylidene)-1,3-propanediamine derivatives.
    Article Snippet: Schiff base and its complexes are being paid more and more interests for their great prospects in biological applications.. We reported here four cobalt(II) complexes [Co3(L)2(HCOO)2] (1), [Co3(L)2(HCOO)2(CH3OH)2]⋅ 2CH3OH (2), [Co3(HL)2(OAc)2(DMF)2] (3) and [Co3(HL)2(HCOO)2(DMF)2]⋅2H2O (4) bearing the bis-Schiff base ligand of bis(5-bromosalicylidene)-1,3-propanediamine (H2L), bis(5-bromosalicylidene)-2-methyl-1,3propanediamine (H2L), bis(5-chlorosalicylidene)-2-hydroxyl-1,3-propanediamine (H3L) and bis(5bromosalicylidene)-2-hydroxyl-1,3-propanediamine (H3L), respectively.. The anti-tumor activities of the four titled complexes were screened on a series of tumor cell lines.

    Article Title: Arsenite increases Cyclin D1 expression through coordinated regulation of the Ca 2+ /NFAT2 and NF-κB pathways via ERK/MAPK in a human uroepithelial cell line.
    Article Snippet: To understand the direct link between Cyclin D1, and nuclear factor of activated T cells 2 (NFAT2) and nuclear factor (NF)-kB in arsenic-treated bladder cells, as well as the association between MAPK and NFAT signaling, we determined whether or not the Ca/NFAT pathway is activated in an arsenic-treated normal urothelial cell line and determined the roles of NFAT and NF-kB signals in the regulation of Cyclin D1 expression.. The SV-40 immortalized human uroepithelial cell line, SV-HUC-1, was treated with NaAsO2 for 24 h (0, 1, 2, 4, 8, and 10 mM) and 10, 20, 30, and 40 weeks (0 and 0.5 mM).. We found that arsenite increased the intracellular Ca levels and induced NFAT2 nuclear translocation after treatment for 24 h. The level of NFAT2 mRNA and expression of total protein and nuclear protein were increased after long-term treatment with 0.5 mM arsenite for 30 and 40 weeks compared to the cells treated for 24 h. In addition, NF-kB p50 and p65 nuclear protein expression increased significantly in cells treated with 2–8 mM arsenite for 24 h, which was consistent with NFAT2 nuclear expression.

    Article Title: SiO 2 particles induce pulmonary fibrosis by modulating NLRP3 through the ROS/Keap1/Nrf2 signaling pathway in rats.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Cell Culture:

    Article Title: Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/Ca 2+ Signaling Pathway
    Article Snippet: After the cells were treated with apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA), mitochondrial membrane potential detection kit and Ca 2+ detection kit (Beyotime Biotechnology, Shanghai, China), the apoptosis rate, mitochondrial membrane potential change and intracellular Ca 2+ content were detected by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

    Article Title: Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress- and Ca 2+ Imbalance-Mediated Endoplasmic Reticulum Stress.
    Article Snippet: Ca2+ kit, 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), and NAC were from Beyotime (Nanjing, China).

    Article Title: ITRAQ-based quantitative proteomic analysis of MG63 in response to HIF-1α inducers.
    Article Snippet: Non-healing fractures constitute a serious clinical problem.. HIF-1α is a crucial regulator in response to hypoxia and is proven to be pivotal in bone growth; however, the mechanism still needs further research.. In this study, iTRAQ was used to study the effects of two HIF-1α inducers on the expression of proteins in MG63 cells.

    Article Title: Sequential construction of vascularized and mineralized bone organoids using engineered ECM-DNA-CPO-based bionic matrix for efficient bone regeneration
    Article Snippet: The calcium ion concentration was measured using a Ca 2+ detection kit (Beyotime, China).

    Article Title: Anticancer activity of four trinuclear cobalt complexes bearing bis(salicylidene)-1,3-propanediamine derivatives.
    Article Snippet: Schiff base and its complexes are being paid more and more interests for their great prospects in biological applications.. We reported here four cobalt(II) complexes [Co3(L)2(HCOO)2] (1), [Co3(L)2(HCOO)2(CH3OH)2]⋅ 2CH3OH (2), [Co3(HL)2(OAc)2(DMF)2] (3) and [Co3(HL)2(HCOO)2(DMF)2]⋅2H2O (4) bearing the bis-Schiff base ligand of bis(5-bromosalicylidene)-1,3-propanediamine (H2L), bis(5-bromosalicylidene)-2-methyl-1,3propanediamine (H2L), bis(5-chlorosalicylidene)-2-hydroxyl-1,3-propanediamine (H3L) and bis(5bromosalicylidene)-2-hydroxyl-1,3-propanediamine (H3L), respectively.. The anti-tumor activities of the four titled complexes were screened on a series of tumor cell lines.

    Article Title: Arsenite increases Cyclin D1 expression through coordinated regulation of the Ca 2+ /NFAT2 and NF-κB pathways via ERK/MAPK in a human uroepithelial cell line.
    Article Snippet: To understand the direct link between Cyclin D1, and nuclear factor of activated T cells 2 (NFAT2) and nuclear factor (NF)-kB in arsenic-treated bladder cells, as well as the association between MAPK and NFAT signaling, we determined whether or not the Ca/NFAT pathway is activated in an arsenic-treated normal urothelial cell line and determined the roles of NFAT and NF-kB signals in the regulation of Cyclin D1 expression.. The SV-40 immortalized human uroepithelial cell line, SV-HUC-1, was treated with NaAsO2 for 24 h (0, 1, 2, 4, 8, and 10 mM) and 10, 20, 30, and 40 weeks (0 and 0.5 mM).. We found that arsenite increased the intracellular Ca levels and induced NFAT2 nuclear translocation after treatment for 24 h. The level of NFAT2 mRNA and expression of total protein and nuclear protein were increased after long-term treatment with 0.5 mM arsenite for 30 and 40 weeks compared to the cells treated for 24 h. In addition, NF-kB p50 and p65 nuclear protein expression increased significantly in cells treated with 2–8 mM arsenite for 24 h, which was consistent with NFAT2 nuclear expression.

    Article Title: SiO 2 particles induce pulmonary fibrosis by modulating NLRP3 through the ROS/Keap1/Nrf2 signaling pathway in rats.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.



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    The effects of Y-27632(day 9) and AA(day 5) on the levels of Ca 2+ and TC of sperm. ( A ) Regulation of Ca 2+ content by 20 μM Y-27632. ( B ) Regulation of TC activity by 20 μM Y-27632. ( C ) Regulation of Ca 2+ content by 100 μM AA. ( D ) Regulation of TC activity by 100 μM AA. ** indicates p < 0.01.
    Ca 2+/Mg 2+ Atpase Detection Kit, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The effects of Y-27632(day 9) and AA(day 5) on the levels of Ca 2+ and TC of sperm. ( A ) Regulation of Ca 2+ content by 20 μM Y-27632. ( B ) Regulation of TC activity by 20 μM Y-27632. ( C ) Regulation of Ca 2+ content by 100 μM AA. ( D ) Regulation of TC activity by 100 μM AA. ** indicates p < 0.01.
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    The effects of Y-27632(day 9) and AA(day 5) on the levels of Ca 2+ and TC of sperm. ( A ) Regulation of Ca 2+ content by 20 μM Y-27632. ( B ) Regulation of TC activity by 20 μM Y-27632. ( C ) Regulation of Ca 2+ content by 100 μM AA. ( D ) Regulation of TC activity by 100 μM AA. ** indicates p < 0.01.
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    Image Search Results


    Transcriptomic results of PRAC-E ameliorating HA. ( A ) Venn diagram of the DEGs from PRAC-E vs. LYSO-6 and LYSO-6 vs. Normal. ( B ) LYSO-6 vs. Normal DEGs volcano plot. ( C ) PRAC-E vs. LYSO-6 DEGs volcano plot. ( D ) Functional enrichment analysis of downregulated DEGs in LYSO-6 vs. Normal. ( E ) Function enrichment analysis of upregulated DEGs in PRAC-E vs. LYSO-6. ( F ) Pathway enrichment analysis of the downregulated DEGs in LYSO-6 vs. Normal. ( G ) Pathway enrichment analysis of upregulated DEGs in PRAC-E vs. LYSO-6. ( H ) Ca 2+ -ATPase activity in liver tissue ( n = 6). Data are presented as the mean ± standard deviation (SD) ( n = 6). ** p < 0.01, *** p < 0.001 vs. LYSO-6.

    Journal: International Journal of Molecular Sciences

    Article Title: Protective Effect of Paeoniae Radix Alba Carbonisata on Hepatic Amyloidosis by Regulating Calcium Homeostasis

    doi: 10.3390/ijms27062582

    Figure Lengend Snippet: Transcriptomic results of PRAC-E ameliorating HA. ( A ) Venn diagram of the DEGs from PRAC-E vs. LYSO-6 and LYSO-6 vs. Normal. ( B ) LYSO-6 vs. Normal DEGs volcano plot. ( C ) PRAC-E vs. LYSO-6 DEGs volcano plot. ( D ) Functional enrichment analysis of downregulated DEGs in LYSO-6 vs. Normal. ( E ) Function enrichment analysis of upregulated DEGs in PRAC-E vs. LYSO-6. ( F ) Pathway enrichment analysis of the downregulated DEGs in LYSO-6 vs. Normal. ( G ) Pathway enrichment analysis of upregulated DEGs in PRAC-E vs. LYSO-6. ( H ) Ca 2+ -ATPase activity in liver tissue ( n = 6). Data are presented as the mean ± standard deviation (SD) ( n = 6). ** p < 0.01, *** p < 0.001 vs. LYSO-6.

    Article Snippet: ALT, AST, ALP, and ultramicro Ca 2+ -ATPase detection kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).

    Techniques: Functional Assay, Activity Assay, Standard Deviation

    PRAC-E modulates calcium homeostasis via the cGMP/PKG/ATP2A1 signaling axis, thereby alleviating inflammation and amyloid deposition. ( A , B ) The dose-dependent ( A ) and time-dependent ( B ) effects of LYSO-6 on NCTC1469 cells. ( C ) Screening of the effective concentration of PRAC-E on LYSO-6-stimulated cells. ( D – F ) The levels of IL-6, TNF-α, and IL-8 in the cell culture supernatant. ( G – I ) The levels of SAA, SAP ( G ), and APO-E ( H ) in NCTC1469 cells. ( J ) CR staining of NCTC1469 cells. ( K ) The dose-dependent effects of BAPTA-AM on NCTC1469 cells. ( L , M ) Representative Fluo-4 AM fluorescence staining and semi-quantification of NCTC1469 cells. ( N ) Ca 2+ -ATPase activity in NCTC1469 cells. ( O ) Intracellular cGMP levels in NCTC1469 cells were measured by ELISA. ( P – R ) Western blotting and semi-quantitative analysis of PDE5A, PKG, and ATP2A1 in NCTC1469 cells. Data are presented as the mean ± SD ( n = 6). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. solvent control group; * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. LYSO-6; ns, no significance.

    Journal: International Journal of Molecular Sciences

    Article Title: Protective Effect of Paeoniae Radix Alba Carbonisata on Hepatic Amyloidosis by Regulating Calcium Homeostasis

    doi: 10.3390/ijms27062582

    Figure Lengend Snippet: PRAC-E modulates calcium homeostasis via the cGMP/PKG/ATP2A1 signaling axis, thereby alleviating inflammation and amyloid deposition. ( A , B ) The dose-dependent ( A ) and time-dependent ( B ) effects of LYSO-6 on NCTC1469 cells. ( C ) Screening of the effective concentration of PRAC-E on LYSO-6-stimulated cells. ( D – F ) The levels of IL-6, TNF-α, and IL-8 in the cell culture supernatant. ( G – I ) The levels of SAA, SAP ( G ), and APO-E ( H ) in NCTC1469 cells. ( J ) CR staining of NCTC1469 cells. ( K ) The dose-dependent effects of BAPTA-AM on NCTC1469 cells. ( L , M ) Representative Fluo-4 AM fluorescence staining and semi-quantification of NCTC1469 cells. ( N ) Ca 2+ -ATPase activity in NCTC1469 cells. ( O ) Intracellular cGMP levels in NCTC1469 cells were measured by ELISA. ( P – R ) Western blotting and semi-quantitative analysis of PDE5A, PKG, and ATP2A1 in NCTC1469 cells. Data are presented as the mean ± SD ( n = 6). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. solvent control group; * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. LYSO-6; ns, no significance.

    Article Snippet: ALT, AST, ALP, and ultramicro Ca 2+ -ATPase detection kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).

    Techniques: Concentration Assay, Cell Culture, Staining, Fluorescence, Activity Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Solvent, Control

    Journal: World Journal of Cardiology

    Article Title: Mechanism of myocardial damage induced by doxorubicin via calumenin-regulated mitochondrial dynamics and the calcium–Cx43 pathway

    doi: 10.4330/wjc.v17.i5.104839

    Figure Lengend Snippet: The concentration of intracellular free Ca 2+ of cardiomyocytes in vivo

    Article Snippet: The intracellular free Ca 2+ in rat cardiac tissues was detected with the intracellular free Ca 2+ detection kit and fluorescence labeling instrument, which were obtained from Shanghai Haohai Biological Technology Co. Ltd. Cardiac tissues were digested to a single-cell suspension, which was treated with reaction reagent and a dye solution.

    Techniques: Concentration Assay

    In vitro effects of the combination hypothermia and MgSO 4 on neurons. (A) The flow chart of neural cell experiments. (B) Cell viability assay (n=6). (C-D) Fluorescence imaging of intracellular Ca 2+ and summary data of relative fulroscence intensity of Ca 2+ in neuron (n=6). scale bars: 20 µm. (E) Summary data of relative fulroscence intensity of ROS in neuron (n=6). (F-G) Flow cytometry and quantification of cell apoptosis rate (n=5). * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Theranostics

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units

    doi: 10.7150/thno.104879

    Figure Lengend Snippet: In vitro effects of the combination hypothermia and MgSO 4 on neurons. (A) The flow chart of neural cell experiments. (B) Cell viability assay (n=6). (C-D) Fluorescence imaging of intracellular Ca 2+ and summary data of relative fulroscence intensity of Ca 2+ in neuron (n=6). scale bars: 20 µm. (E) Summary data of relative fulroscence intensity of ROS in neuron (n=6). (F-G) Flow cytometry and quantification of cell apoptosis rate (n=5). * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Techniques: In Vitro, Viability Assay, Fluorescence, Imaging, Flow Cytometry

    In vitro effects of the combination of hypothermia and MgSO 4 on microvessel EC. (A) The flow chart of microvessel EC experiments. (B) Cell viability assay. (C-D) Fluorescence imaging of intracellular Ca 2+ and summary data of relative fluorescence intensity of Ca 2+ in ECs. Scale bars: 20 µm. (E) Summary data of relative fluorescence intensity of ROS in ECs. (F-G) Fluorescence imaging and quantification of cellular ZO-1 expression. n=6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Theranostics

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units

    doi: 10.7150/thno.104879

    Figure Lengend Snippet: In vitro effects of the combination of hypothermia and MgSO 4 on microvessel EC. (A) The flow chart of microvessel EC experiments. (B) Cell viability assay. (C-D) Fluorescence imaging of intracellular Ca 2+ and summary data of relative fluorescence intensity of Ca 2+ in ECs. Scale bars: 20 µm. (E) Summary data of relative fluorescence intensity of ROS in ECs. (F-G) Fluorescence imaging and quantification of cellular ZO-1 expression. n=6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Techniques: In Vitro, Viability Assay, Fluorescence, Imaging, Expressing

    In vitro effects of the combination of hypothermia and MgSO 4 on pericytes. (A) The flow chart of pericyte experiments. (B) Cell viability assay. (C) Quantitative analysis of the area of collagen gel. (D) Quantitative analysis of relative fluorescence intensity of Ca 2+ in pericytes. (E) Fluorescence imaging of intracellular Ca 2+ after reoxygenation 24 h. scale bars: 20 µm. n=6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Theranostics

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units

    doi: 10.7150/thno.104879

    Figure Lengend Snippet: In vitro effects of the combination of hypothermia and MgSO 4 on pericytes. (A) The flow chart of pericyte experiments. (B) Cell viability assay. (C) Quantitative analysis of the area of collagen gel. (D) Quantitative analysis of relative fluorescence intensity of Ca 2+ in pericytes. (E) Fluorescence imaging of intracellular Ca 2+ after reoxygenation 24 h. scale bars: 20 µm. n=6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Techniques: In Vitro, Viability Assay, Fluorescence, Imaging

    MgSO 4 as a novel hypothermia infusion solution for promoting AIS recovery by regulating Ca 2+ in NVUs. After I/R injury, the infarction region undergoes severe neural injury, BBB breakdown and microvessel occlusion, which were triggered by Ca 2+ influx into neurons, cerebral microvessel ECs and pericytes. IA-SCSI cooled the brain and promoted partial recovery from neural injury and BBB breakdown but could not rescue microvessel occlusion. In vitro cell experiments confirmed that hypothermia could partially inhibit Ca 2+ influx into neurons and cerebral microvessel ECs but did not affect the total amount of calcium ions in pericytes because hypothermia increased Ca 2+ influx through TRPM8. Moreover, IA-SCMI demonstrated significant recovery from neural injury and BBB breakdown, and enhanced microvessel circulation. The combination of hypothermia and Mg 2+ exerted stronger inhibitory effects on Ca 2+ influx compared with hypothermia alone in NVU cells. MCA, middle cerebral artery; ICA, internal carotid artery; ECA, external carotid artery; CCA, common carotid artery; IA-SCSI, intra-artery selective cooling saline infusion; IA-SCMI, intra-artery selective cooling MgSO 4 infusion; BBB, brain-blood barrier.

    Journal: Theranostics

    Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units

    doi: 10.7150/thno.104879

    Figure Lengend Snippet: MgSO 4 as a novel hypothermia infusion solution for promoting AIS recovery by regulating Ca 2+ in NVUs. After I/R injury, the infarction region undergoes severe neural injury, BBB breakdown and microvessel occlusion, which were triggered by Ca 2+ influx into neurons, cerebral microvessel ECs and pericytes. IA-SCSI cooled the brain and promoted partial recovery from neural injury and BBB breakdown but could not rescue microvessel occlusion. In vitro cell experiments confirmed that hypothermia could partially inhibit Ca 2+ influx into neurons and cerebral microvessel ECs but did not affect the total amount of calcium ions in pericytes because hypothermia increased Ca 2+ influx through TRPM8. Moreover, IA-SCMI demonstrated significant recovery from neural injury and BBB breakdown, and enhanced microvessel circulation. The combination of hypothermia and Mg 2+ exerted stronger inhibitory effects on Ca 2+ influx compared with hypothermia alone in NVU cells. MCA, middle cerebral artery; ICA, internal carotid artery; ECA, external carotid artery; CCA, common carotid artery; IA-SCSI, intra-artery selective cooling saline infusion; IA-SCMI, intra-artery selective cooling MgSO 4 infusion; BBB, brain-blood barrier.

    Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

    Techniques: Infusion Solution, In Vitro, Saline

    Effects of HLTP on MMP and Ca 2+ ( (A) Flow cytometry to detect cellular MMP, F = 442.9, P < 0.0001; (B) Flow cytometry to detect intracellular Ca 2+ concentration, F = 497.1, P < 0.0001; (C) Fluorescence microscopy to observe intracellular Ca 2+ concentration, Normal ( F = 93.4, P < 0.0001), Fluo-4 ( F = 86.7, P < 0.0001)) (Note: Compared with the Control group: # P < 0.05 and ## P < 0.01; Compared with the Model group: * P < 0.05 and ** P < 0.01; mean ± SD, n = 3; A–C: DFn = 4, DFd = 10; Magnification ×20. Flow cytometry and fluorescence microscopy were used to detect the MMP and Ca 2+ ).

    Journal: Frontiers in Pharmacology

    Article Title: Neuroprotective effects of total phenolics from Hemerocallis citrina Baroni leaves through the PI3K/AKT pathway

    doi: 10.3389/fphar.2024.1370619

    Figure Lengend Snippet: Effects of HLTP on MMP and Ca 2+ ( (A) Flow cytometry to detect cellular MMP, F = 442.9, P < 0.0001; (B) Flow cytometry to detect intracellular Ca 2+ concentration, F = 497.1, P < 0.0001; (C) Fluorescence microscopy to observe intracellular Ca 2+ concentration, Normal ( F = 93.4, P < 0.0001), Fluo-4 ( F = 86.7, P < 0.0001)) (Note: Compared with the Control group: # P < 0.05 and ## P < 0.01; Compared with the Model group: * P < 0.05 and ** P < 0.01; mean ± SD, n = 3; A–C: DFn = 4, DFd = 10; Magnification ×20. Flow cytometry and fluorescence microscopy were used to detect the MMP and Ca 2+ ).

    Article Snippet: ROS (S0033S) and Ca 2+ (S1061S) detection kits were obtained from Beyotime.

    Techniques: Flow Cytometry, Concentration Assay, Fluorescence, Microscopy, Control

    The effects of Y-27632(day 9) and AA(day 5) on the levels of Ca 2+ and TC of sperm. ( A ) Regulation of Ca 2+ content by 20 μM Y-27632. ( B ) Regulation of TC activity by 20 μM Y-27632. ( C ) Regulation of Ca 2+ content by 100 μM AA. ( D ) Regulation of TC activity by 100 μM AA. ** indicates p < 0.01.

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures

    doi: 10.3390/ani14101441

    Figure Lengend Snippet: The effects of Y-27632(day 9) and AA(day 5) on the levels of Ca 2+ and TC of sperm. ( A ) Regulation of Ca 2+ content by 20 μM Y-27632. ( B ) Regulation of TC activity by 20 μM Y-27632. ( C ) Regulation of Ca 2+ content by 100 μM AA. ( D ) Regulation of TC activity by 100 μM AA. ** indicates p < 0.01.

    Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

    Techniques: Activity Assay