Journal: International Journal of Molecular Sciences

Article Title: Protective Effect of Paeoniae Radix Alba Carbonisata on Hepatic Amyloidosis by Regulating Calcium Homeostasis

doi: 10.3390/ijms27062582

Figure Lengend Snippet: Transcriptomic results of PRAC-E ameliorating HA. ( A ) Venn diagram of the DEGs from PRAC-E vs. LYSO-6 and LYSO-6 vs. Normal. ( B ) LYSO-6 vs. Normal DEGs volcano plot. ( C ) PRAC-E vs. LYSO-6 DEGs volcano plot. ( D ) Functional enrichment analysis of downregulated DEGs in LYSO-6 vs. Normal. ( E ) Function enrichment analysis of upregulated DEGs in PRAC-E vs. LYSO-6. ( F ) Pathway enrichment analysis of the downregulated DEGs in LYSO-6 vs. Normal. ( G ) Pathway enrichment analysis of upregulated DEGs in PRAC-E vs. LYSO-6. ( H ) Ca 2+ -ATPase activity in liver tissue ( n = 6). Data are presented as the mean ± standard deviation (SD) ( n = 6). ** p < 0.01, *** p < 0.001 vs. LYSO-6.

Article Snippet: ALT, AST, ALP, and ultramicro Ca 2+ -ATPase detection kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).

Techniques: Functional Assay, Activity Assay, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Protective Effect of Paeoniae Radix Alba Carbonisata on Hepatic Amyloidosis by Regulating Calcium Homeostasis

doi: 10.3390/ijms27062582

Figure Lengend Snippet: PRAC-E modulates calcium homeostasis via the cGMP/PKG/ATP2A1 signaling axis, thereby alleviating inflammation and amyloid deposition. ( A , B ) The dose-dependent ( A ) and time-dependent ( B ) effects of LYSO-6 on NCTC1469 cells. ( C ) Screening of the effective concentration of PRAC-E on LYSO-6-stimulated cells. ( D – F ) The levels of IL-6, TNF-α, and IL-8 in the cell culture supernatant. ( G – I ) The levels of SAA, SAP ( G ), and APO-E ( H ) in NCTC1469 cells. ( J ) CR staining of NCTC1469 cells. ( K ) The dose-dependent effects of BAPTA-AM on NCTC1469 cells. ( L , M ) Representative Fluo-4 AM fluorescence staining and semi-quantification of NCTC1469 cells. ( N ) Ca 2+ -ATPase activity in NCTC1469 cells. ( O ) Intracellular cGMP levels in NCTC1469 cells were measured by ELISA. ( P – R ) Western blotting and semi-quantitative analysis of PDE5A, PKG, and ATP2A1 in NCTC1469 cells. Data are presented as the mean ± SD ( n = 6). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. solvent control group; * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. LYSO-6; ns, no significance.

Article Snippet: ALT, AST, ALP, and ultramicro Ca 2+ -ATPase detection kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).

Techniques: Concentration Assay, Cell Culture, Staining, Fluorescence, Activity Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Solvent, Control

Journal: World Journal of Cardiology

Article Title: Mechanism of myocardial damage induced by doxorubicin via calumenin-regulated mitochondrial dynamics and the calcium–Cx43 pathway

doi: 10.4330/wjc.v17.i5.104839

Figure Lengend Snippet: The concentration of intracellular free Ca 2+ of cardiomyocytes in vivo

Article Snippet: The intracellular free Ca 2+ in rat cardiac tissues was detected with the intracellular free Ca 2+ detection kit and fluorescence labeling instrument, which were obtained from Shanghai Haohai Biological Technology Co. Ltd. Cardiac tissues were digested to a single-cell suspension, which was treated with reaction reagent and a dye solution.

Techniques: Concentration Assay

Journal: Theranostics

Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units

doi: 10.7150/thno.104879

Figure Lengend Snippet: In vitro effects of the combination hypothermia and MgSO 4 on neurons. (A) The flow chart of neural cell experiments. (B) Cell viability assay (n=6). (C-D) Fluorescence imaging of intracellular Ca 2+ and summary data of relative fulroscence intensity of Ca 2+ in neuron (n=6). scale bars: 20 µm. (E) Summary data of relative fulroscence intensity of ROS in neuron (n=6). (F-G) Flow cytometry and quantification of cell apoptosis rate (n=5). * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

Techniques: In Vitro, Viability Assay, Fluorescence, Imaging, Flow Cytometry

Journal: Theranostics

Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units

doi: 10.7150/thno.104879

Figure Lengend Snippet: In vitro effects of the combination of hypothermia and MgSO 4 on microvessel EC. (A) The flow chart of microvessel EC experiments. (B) Cell viability assay. (C-D) Fluorescence imaging of intracellular Ca 2+ and summary data of relative fluorescence intensity of Ca 2+ in ECs. Scale bars: 20 µm. (E) Summary data of relative fluorescence intensity of ROS in ECs. (F-G) Fluorescence imaging and quantification of cellular ZO-1 expression. n=6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

Techniques: In Vitro, Viability Assay, Fluorescence, Imaging, Expressing

Journal: Theranostics

Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units

doi: 10.7150/thno.104879

Figure Lengend Snippet: In vitro effects of the combination of hypothermia and MgSO 4 on pericytes. (A) The flow chart of pericyte experiments. (B) Cell viability assay. (C) Quantitative analysis of the area of collagen gel. (D) Quantitative analysis of relative fluorescence intensity of Ca 2+ in pericytes. (E) Fluorescence imaging of intracellular Ca 2+ after reoxygenation 24 h. scale bars: 20 µm. n=6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

Techniques: In Vitro, Viability Assay, Fluorescence, Imaging

Journal: Theranostics

Article Title: MgSO 4 as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca 2+ regulation of neurovascular units

doi: 10.7150/thno.104879

Figure Lengend Snippet: MgSO 4 as a novel hypothermia infusion solution for promoting AIS recovery by regulating Ca 2+ in NVUs. After I/R injury, the infarction region undergoes severe neural injury, BBB breakdown and microvessel occlusion, which were triggered by Ca 2+ influx into neurons, cerebral microvessel ECs and pericytes. IA-SCSI cooled the brain and promoted partial recovery from neural injury and BBB breakdown but could not rescue microvessel occlusion. In vitro cell experiments confirmed that hypothermia could partially inhibit Ca 2+ influx into neurons and cerebral microvessel ECs but did not affect the total amount of calcium ions in pericytes because hypothermia increased Ca 2+ influx through TRPM8. Moreover, IA-SCMI demonstrated significant recovery from neural injury and BBB breakdown, and enhanced microvessel circulation. The combination of hypothermia and Mg 2+ exerted stronger inhibitory effects on Ca 2+ influx compared with hypothermia alone in NVU cells. MCA, middle cerebral artery; ICA, internal carotid artery; ECA, external carotid artery; CCA, common carotid artery; IA-SCSI, intra-artery selective cooling saline infusion; IA-SCMI, intra-artery selective cooling MgSO 4 infusion; BBB, brain-blood barrier.

Article Snippet: Intracellular Ca 2+ imaging and quantification was conducted using a Fluo-4 Ca 2+ detection kit (Beyotime, China).

Techniques: Infusion Solution, In Vitro, Saline

Journal: Frontiers in Pharmacology

Article Title: Neuroprotective effects of total phenolics from Hemerocallis citrina Baroni leaves through the PI3K/AKT pathway

doi: 10.3389/fphar.2024.1370619

Figure Lengend Snippet: Effects of HLTP on MMP and Ca 2+ ( (A) Flow cytometry to detect cellular MMP, F = 442.9, P < 0.0001; (B) Flow cytometry to detect intracellular Ca 2+ concentration, F = 497.1, P < 0.0001; (C) Fluorescence microscopy to observe intracellular Ca 2+ concentration, Normal ( F = 93.4, P < 0.0001), Fluo-4 ( F = 86.7, P < 0.0001)) (Note: Compared with the Control group: # P < 0.05 and ## P < 0.01; Compared with the Model group: * P < 0.05 and ** P < 0.01; mean ± SD, n = 3; A–C: DFn = 4, DFd = 10; Magnification ×20. Flow cytometry and fluorescence microscopy were used to detect the MMP and Ca 2+ ).

Article Snippet: ROS (S0033S) and Ca 2+ (S1061S) detection kits were obtained from Beyotime.

Techniques: Flow Cytometry, Concentration Assay, Fluorescence, Microscopy, Control

Journal: Animals : an Open Access Journal from MDPI

Article Title: Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures

doi: 10.3390/ani14101441

Figure Lengend Snippet: The effects of Y-27632(day 9) and AA(day 5) on the levels of Ca 2+ and TC of sperm. ( A ) Regulation of Ca 2+ content by 20 μM Y-27632. ( B ) Regulation of TC activity by 20 μM Y-27632. ( C ) Regulation of Ca 2+ content by 100 μM AA. ( D ) Regulation of TC activity by 100 μM AA. ** indicates p < 0.01.

Article Snippet: The Ca 2+ content in cells was rapidly and quantitatively detected by colorimetry using the Ca 2+ detection kit (S1063S, Shanghai Beyotime Biology Technology Co., Ltd.).

Techniques: Activity Assay