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shank 1a (Neuromics)

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Neuromics shank 1a

A–C : A. Image of a retinal section immunostained for Shank <t>1A.</t> B. Mouse YFP-16 line vertical retinal section. C. Shank 1A (red) immunolabeling and YFP (yellow). Shank1A expression is restricted to the OPL and IPL. A regular pattern of Shank 1A immunolabeling appears in the OPL, which is indicative of cone photoreceptor terminals. D–E : High magnification zoom of the OPL demonstrates that Shank 1A puncta (red) are distal to the dendrite tips (yellow) of YFP labeled cone bipolar cells, suggesting that Shank 1A is expressed presynaptic to the YFP cone bipolar cell dendrite. G–L : High magnification zoom of the IPL demonstrates that Shank 1A puncta are likely expressed postsynaptically to bipolar cell terminals. G. Shank 1A immunoreactive puncta. H. YFP labeled neurons and processes within the IPL region. I. PKCα labeled rod bipolar cell axons and terminals. J. Combined Shank 1A (red) and PKCα (blue) immunolabeling illustrate that shank 1A puncta are postsynaptic to rod bipolar cell terminals in the IPL. K. Combined Shank 1A (red) immunolabeling and YFP (yellow) in the IPL demonstrate that Shank 1A puncta are postsynaptic to cone bipolar cell terminals in the IPL. L. Combined triple fluorescent image of Shank 1A (red), PKCα (blue), and YFP (yellow) in the IPL. OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, and GCL = ganglion cell layer. Scale bars = 10 µm.

Shank 1a, supplied by Neuromics, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+terminus/SHANK1a+C-terminus/pmc03440378-154-68-74
Average 93 stars, based on 6 article reviews

shank 1a - by Bioz Stars, 2026-11

93/100 stars

Images

1) Product Images from "Association of Shank 1A Scaffolding Protein with Cone Photoreceptor Terminals in the Mammalian Retina"

Article Title: Association of Shank 1A Scaffolding Protein with Cone Photoreceptor Terminals in the Mammalian Retina

Journal: PLoS ONE

doi: 10.1371/journal.pone.0043463

A–C : A. Image of a retinal section immunostained for Shank 1A. B. Mouse YFP-16 line vertical retinal section. C. Shank 1A (red) immunolabeling and YFP (yellow). Shank1A expression is restricted to the OPL and IPL. A regular pattern of Shank 1A immunolabeling appears in the OPL, which is indicative of cone photoreceptor terminals. D–E : High magnification zoom of the OPL demonstrates that Shank 1A puncta (red) are distal to the dendrite tips (yellow) of YFP labeled cone bipolar cells, suggesting that Shank 1A is expressed presynaptic to the YFP cone bipolar cell dendrite. G–L : High magnification zoom of the IPL demonstrates that Shank 1A puncta are likely expressed postsynaptically to bipolar cell terminals. G. Shank 1A immunoreactive puncta. H. YFP labeled neurons and processes within the IPL region. I. PKCα labeled rod bipolar cell axons and terminals. J. Combined Shank 1A (red) and PKCα (blue) immunolabeling illustrate that shank 1A puncta are postsynaptic to rod bipolar cell terminals in the IPL. K. Combined Shank 1A (red) immunolabeling and YFP (yellow) in the IPL demonstrate that Shank 1A puncta are postsynaptic to cone bipolar cell terminals in the IPL. L. Combined triple fluorescent image of Shank 1A (red), PKCα (blue), and YFP (yellow) in the IPL. OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, and GCL = ganglion cell layer. Scale bars = 10 µm.

Figure Legend Snippet: A–C : A. Image of a retinal section immunostained for Shank 1A. B. Mouse YFP-16 line vertical retinal section. C. Shank 1A (red) immunolabeling and YFP (yellow). Shank1A expression is restricted to the OPL and IPL. A regular pattern of Shank 1A immunolabeling appears in the OPL, which is indicative of cone photoreceptor terminals. D–E : High magnification zoom of the OPL demonstrates that Shank 1A puncta (red) are distal to the dendrite tips (yellow) of YFP labeled cone bipolar cells, suggesting that Shank 1A is expressed presynaptic to the YFP cone bipolar cell dendrite. G–L : High magnification zoom of the IPL demonstrates that Shank 1A puncta are likely expressed postsynaptically to bipolar cell terminals. G. Shank 1A immunoreactive puncta. H. YFP labeled neurons and processes within the IPL region. I. PKCα labeled rod bipolar cell axons and terminals. J. Combined Shank 1A (red) and PKCα (blue) immunolabeling illustrate that shank 1A puncta are postsynaptic to rod bipolar cell terminals in the IPL. K. Combined Shank 1A (red) immunolabeling and YFP (yellow) in the IPL demonstrate that Shank 1A puncta are postsynaptic to cone bipolar cell terminals in the IPL. L. Combined triple fluorescent image of Shank 1A (red), PKCα (blue), and YFP (yellow) in the IPL. OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, and GCL = ganglion cell layer. Scale bars = 10 µm.

Techniques Used: Immunolabeling, Expressing, Labeling

A : Shank 1A immunoreactivity in wild type mouse retina, punctate antibody labeling in the OPL in the outer retina and throughout the IPL. B : No Shank 1A immunoreactivity was found in retinal sections obtained from animals where the Shank 1 gene was deleted. (OS = outer segment, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, and GCL = ganglion cell layer). Scale bar is 10 µm.

Figure Legend Snippet: A : Shank 1A immunoreactivity in wild type mouse retina, punctate antibody labeling in the OPL in the outer retina and throughout the IPL. B : No Shank 1A immunoreactivity was found in retinal sections obtained from animals where the Shank 1 gene was deleted. (OS = outer segment, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, and GCL = ganglion cell layer). Scale bar is 10 µm.

Techniques Used: Antibody Labeling

A–D : Zoomed confocal region of the OPL immunostained with rabbit anti-Shank1A and mouse anti-PSD-95 antibodies. A. Shank 1A puncta labeling in the OPL in the mouse retina. B. PSD-95 immunolabels rod and cone photoreceptor terminals in the OPL. C. YFP labeled dendrites from cone bipolar cells in the OPL. D. Merged confocal image showing Shank 1A (red), PSD-95 (blue), and YFP (yellow). E–J : High magnification single plane confocal images of Shank 1A and PSD-95 in the mouse thy 1.2 YFP 16 line retina (100× objective, N.A. 1.3). E. Shank1 A (red). F. PSD-95 (blue). G. YFP (yellow). H. Merged image of YFP and Shank 1A, YFP and Shank 1A puncta are not co-localized at cone bipolar cell dendrites (see arrowheads, shank fluorescence above YFP dendrite. I. Merged image of Shank 1A and PSD-95, the two immunoreactivities (pink) indicate co-localization of Shank 1a and PSD-95 (see arrows which indicate that Shank 1A is co-localized with PSD-95). Arrows indicate location of Shank 1A immunoractive puncta co-localized with PSD-95. J. Merged image of inset Shank 1A (red), PSD-95 (blue), and YFP (yellow). Primary antibodies were detected using a secondary goat anti-rabbit Alexa 568 IgG for Shank 1A, and a goat anti-mouse Alexa 633 IgG to PSD-95. Scale bar is 5 µm.

Figure Legend Snippet: A–D : Zoomed confocal region of the OPL immunostained with rabbit anti-Shank1A and mouse anti-PSD-95 antibodies. A. Shank 1A puncta labeling in the OPL in the mouse retina. B. PSD-95 immunolabels rod and cone photoreceptor terminals in the OPL. C. YFP labeled dendrites from cone bipolar cells in the OPL. D. Merged confocal image showing Shank 1A (red), PSD-95 (blue), and YFP (yellow). E–J : High magnification single plane confocal images of Shank 1A and PSD-95 in the mouse thy 1.2 YFP 16 line retina (100× objective, N.A. 1.3). E. Shank1 A (red). F. PSD-95 (blue). G. YFP (yellow). H. Merged image of YFP and Shank 1A, YFP and Shank 1A puncta are not co-localized at cone bipolar cell dendrites (see arrowheads, shank fluorescence above YFP dendrite. I. Merged image of Shank 1A and PSD-95, the two immunoreactivities (pink) indicate co-localization of Shank 1a and PSD-95 (see arrows which indicate that Shank 1A is co-localized with PSD-95). Arrows indicate location of Shank 1A immunoractive puncta co-localized with PSD-95. J. Merged image of inset Shank 1A (red), PSD-95 (blue), and YFP (yellow). Primary antibodies were detected using a secondary goat anti-rabbit Alexa 568 IgG for Shank 1A, and a goat anti-mouse Alexa 633 IgG to PSD-95. Scale bar is 5 µm.

Techniques Used: Labeling, Fluorescence

High magnification zoom of a region in the OPL. A–H : Confocal images from the mouse thy-1.2 YFP 16 line immunolabeled with Shank 1A and CtBP2 (a homologue of RIBEYE), a marker of synaptic ribbons in mammalian retina. A. Shank 1A (red). B. CtBP2 (blue). C. A combined fluorescence image showing Shank 1A (red) and CtBP2 (blue). D. A schematic illustrating the zoomed confocal image in H, showing that the Shank 1A puncta (red) is located distal and adjacent to the tips of the YFP dendrite (yellow), and are surrounded by CtBP2 labeled synaptic ribbon protein structures (blue). E. YFP (yellow) F. A combined fluorescence image showing Shank 1A (red) and YFP (yellow). G. A combined fluorescence image showing CtBP2 (blue) and YFP (yellow). H. A combined triple labeled image showing Shank 1A (red), CtBP2 (blue), and YFP (yellow). Scale bar = 10 µm.

Figure Legend Snippet: High magnification zoom of a region in the OPL. A–H : Confocal images from the mouse thy-1.2 YFP 16 line immunolabeled with Shank 1A and CtBP2 (a homologue of RIBEYE), a marker of synaptic ribbons in mammalian retina. A. Shank 1A (red). B. CtBP2 (blue). C. A combined fluorescence image showing Shank 1A (red) and CtBP2 (blue). D. A schematic illustrating the zoomed confocal image in H, showing that the Shank 1A puncta (red) is located distal and adjacent to the tips of the YFP dendrite (yellow), and are surrounded by CtBP2 labeled synaptic ribbon protein structures (blue). E. YFP (yellow) F. A combined fluorescence image showing Shank 1A (red) and YFP (yellow). G. A combined fluorescence image showing CtBP2 (blue) and YFP (yellow). H. A combined triple labeled image showing Shank 1A (red), CtBP2 (blue), and YFP (yellow). Scale bar = 10 µm.

Techniques Used: Immunolabeling, Marker, Fluorescence, Labeling

A–D : Combined labeling of Shank 1A, PNA, and YFP in the mouse thy-1.2 YFP 16 line vertical retinal section. A. Shank 1A labels both the OPL and IPL. The immunoreactive puncta in the OPL are indicative of cone photoreceptor labeling. B. PNA conjugated rhodamine labels the inner and outer segments of cone photoreceptors, and cone photoreceptor terminals in the OPL. C. YFP fluorescence is present in bipolar, amacrine, and ganglion cells. D. Combined triple fluorescence channel image of PNA (red), Shank 1A (blue), and YFP (yellow). A box is drawn of a region in the OPL and high magnification images are shown in E–H. E–H : High magnification zoom of a region in the OPL from D. E. Shank 1A (blue). F. PNA (red). G. YFP labeled cone bipolar cells and their dendrites (yellow). H. A combined triple labeled fluorescent image showing that Shank 1A (blue) is expressed at the same site as PNA (red) above the YFP cone bipolar cell dendrites (yellow). OS = outer segment, IS = inner segment, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, and GCL = ganglion cell layer. Scale bars is 10 µm.

Figure Legend Snippet: A–D : Combined labeling of Shank 1A, PNA, and YFP in the mouse thy-1.2 YFP 16 line vertical retinal section. A. Shank 1A labels both the OPL and IPL. The immunoreactive puncta in the OPL are indicative of cone photoreceptor labeling. B. PNA conjugated rhodamine labels the inner and outer segments of cone photoreceptors, and cone photoreceptor terminals in the OPL. C. YFP fluorescence is present in bipolar, amacrine, and ganglion cells. D. Combined triple fluorescence channel image of PNA (red), Shank 1A (blue), and YFP (yellow). A box is drawn of a region in the OPL and high magnification images are shown in E–H. E–H : High magnification zoom of a region in the OPL from D. E. Shank 1A (blue). F. PNA (red). G. YFP labeled cone bipolar cells and their dendrites (yellow). H. A combined triple labeled fluorescent image showing that Shank 1A (blue) is expressed at the same site as PNA (red) above the YFP cone bipolar cell dendrites (yellow). OS = outer segment, IS = inner segment, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, and GCL = ganglion cell layer. Scale bars is 10 µm.

Techniques Used: Labeling, Fluorescence

A–C : A. Shank 1A B. WGA labeling at the OPL. Arrowheads indicate WGA rod spherule labeling, and arrows indicate the location of WGA labeling of cone pedicles. C. YFP cone bipolar cell dendrites. D–F : Shank 1A (blue) and WGA (red) co-localize at cone terminals in the OPL. G–I: YFP cone bipolar cell dendrites (yellow) synapse with WGA (red) labeled cone terminals, which cradle Shank 1A immunoreactive puncta (blue). Scale bar is 10 µm.

Figure Legend Snippet: A–C : A. Shank 1A B. WGA labeling at the OPL. Arrowheads indicate WGA rod spherule labeling, and arrows indicate the location of WGA labeling of cone pedicles. C. YFP cone bipolar cell dendrites. D–F : Shank 1A (blue) and WGA (red) co-localize at cone terminals in the OPL. G–I: YFP cone bipolar cell dendrites (yellow) synapse with WGA (red) labeled cone terminals, which cradle Shank 1A immunoreactive puncta (blue). Scale bar is 10 µm.

Techniques Used: Labeling

A–D : Shank 1A is co-localized with glycogen phsophorylase (cone photoreceptor marker). A–D : Combined labeling of Shank 1A, glycogen phosphorylase, and YFP in a vertical retinal section. A. Shank 1A labels both the OPL and IPL. The immunoreactive puncta in the OPL are indicative of cone photoreceptor labeling. B. YFP fluorescence is present in bipolar, amacrine, and ganglion cells. C. Glycogen phosphorylase (GP), a cone photoreceptor marker strongly labels the cone terminals with faint labeling of bipolar cell bodies and their axons. D. Combined triple label fluorescence image of Shank 1A (red), YFP (yellow), and glycogen phosphorylase (GP) (blue), and. A box is drawn of a region in the OPL and high magnification images are shown in E–J. E–J : High magnification zoom of a region in the OPL from the inset above in . E. Shank 1A. F. Merge of Shank 1A (red) and YFP (yellow), showing that Shank 1A (red) is expressed above the cone bipolar cell dendrites (yellow). G. Glycogen phosphorylase (GP). H. Merge of Shank 1A (red) and Glycogen phosphorylase (GP), the two immunoreactivities (pink) indicate co-localization of Shank 1a and Glycogenphosphorylase (GP). I. YFP labeled cone bipolar cells and their dendrites. J. Combined image of Shank 1A (red), glycogen phosphorylase (blue), and YFP cone bipolar cell dendrites (yellow). OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, and GCL = ganglion cell layer. Scale bar is 10 µm.

Figure Legend Snippet: A–D : Shank 1A is co-localized with glycogen phsophorylase (cone photoreceptor marker). A–D : Combined labeling of Shank 1A, glycogen phosphorylase, and YFP in a vertical retinal section. A. Shank 1A labels both the OPL and IPL. The immunoreactive puncta in the OPL are indicative of cone photoreceptor labeling. B. YFP fluorescence is present in bipolar, amacrine, and ganglion cells. C. Glycogen phosphorylase (GP), a cone photoreceptor marker strongly labels the cone terminals with faint labeling of bipolar cell bodies and their axons. D. Combined triple label fluorescence image of Shank 1A (red), YFP (yellow), and glycogen phosphorylase (GP) (blue), and. A box is drawn of a region in the OPL and high magnification images are shown in E–J. E–J : High magnification zoom of a region in the OPL from the inset above in . E. Shank 1A. F. Merge of Shank 1A (red) and YFP (yellow), showing that Shank 1A (red) is expressed above the cone bipolar cell dendrites (yellow). G. Glycogen phosphorylase (GP). H. Merge of Shank 1A (red) and Glycogen phosphorylase (GP), the two immunoreactivities (pink) indicate co-localization of Shank 1a and Glycogenphosphorylase (GP). I. YFP labeled cone bipolar cells and their dendrites. J. Combined image of Shank 1A (red), glycogen phosphorylase (blue), and YFP cone bipolar cell dendrites (yellow). OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, and GCL = ganglion cell layer. Scale bar is 10 µm.

Techniques Used: Marker, Labeling, Fluorescence

Figure Legend Snippet: Antibodies and Lectins.

Techniques Used: Sequencing, Generated, Binding Assay, Recombinant, Transduction, Plasmid Preparation

Labeling:

Article Title: A dual‐AAV approach restores fast exocytosis and partially rescues auditory function in deaf otoferlin knock‐out mice
Article Snippet: .. The following primary antibodies were used: mouse IgG1 anti‐otoferlin [13A9] (#ab53233, Abcam; 1:300) for labeling the N‐terminal part of otoferlin, rabbit IgG anti‐otoferlin (custom made; 1:100) for labeling the C‐terminal part of otoferlin, goat IgG anti‐calbindin D28K [C‐20] (#sc‐7691, Santa Cruz Biotechnology; 1:150) to visualize OHCs and IHCs, guinea pig anti‐Vglut3 (#135204, Synaptic Systems; 1:300) to visualize IHCs, chicken IgY anti‐GFP (#ab13970, Abcam, 1:500), rabbit IgG anti‐Shank1a [C‐terminal] (#RA19016, Neuromics; 1:300) for labeling the postsynaptic density, and goat IgG anti‐CtBP2 [E‐16] (#sc‐5967, Santa Cruz Biotechnology; 1:100) and mouse IgG1 anti‐CtBP2 [C‐16] (#612044, BD Biosciences; 1:50) for labeling synaptic ribbons. .. The following secondary antibodies were used: Alexa Fluor 594‐ and Alexa Fluor 568‐conjugated donkey anti‐mouse IgG (#A21203, #A10037, Thermo Fisher Scientific, 1:200), Alexa Fluor 405‐conjugated donkey anti‐mouse IgG (#ab175658, Abcam, 1:200), Alexa Fluor 647‐ and Alexa Fluor 594‐conjugated donkey anti‐rabbit IgG (#A31573, #A21207, Thermo Fisher Scientific, 1:200), DyLight 405‐conjugated donkey anti‐goat IgG (#705‐475‐003, Jackson ImmunoResearch, 1:200), Alexa Fluor 594‐conjugated donkey anti‐goat IgG (#A11058, Thermo Fisher Scientific, 1:200), and Alexa Fluor 488‐conjugated donkey anti‐chicken IgY (#703‐545‐155, Jackson ImmunoResearch, 1:200).

Article Title: A dual-AAV approach restores fast exocytosis and partially rescues auditory function in deaf otoferlin knock-out mice.
Article Snippet: .. The following primary antibodies were used: mouse IgG1 anti-otoferlin [13A9] (#ab53233, Abcam; 1:300) for labeling the N-terminal part of otoferlin, rabbit IgG antiotoferlin (custom made; 1:100) for labeling the C-terminal part of otoferlin, goat IgG anti-calbindin D28K [C-20] (#sc-7691, Santa Cruz Biotechnology; 1:150) to visualize OHCs and IHCs, guinea pig anti-Vglut3 (#135204, Synaptic Systems; 1:300) to visualize IHCs, chicken IgY anti-GFP (#ab13970, Abcam, 1:500), rabbit IgG anti-Shank1a [C-terminal] (#RA19016, Neuromics; 1:300) for labeling the postsynaptic density, and goat IgG anti-CtBP2 [E-16] (#sc-5967, Santa Cruz Biotechnology; 1:100) and mouse IgG1 antiCtBP2 [C-16] (#612044, BD Biosciences; 1:50) for labeling synaptic ribbons. .. The following secondary antibodies were used: Alexa Fluor 594- and Alexa Fluor 568-conjugated donkey anti-mouse IgG (#A21203, #A10037, Thermo Fisher Scientific, 1:200), Alexa Fluor 405-conjugated donkey anti-mouse IgG (#ab175658, Abcam, 1:200), Alexa Fluor 647- and Alexa Fluor 594-conjugated donkey anti-rabbit IgG (#A31573, #A21207, Thermo Fisher Scientific, 1:200), DyLight 405-conjugated donkey anti-goat IgG (#705-475- 003, Jackson ImmunoResearch, 1:200), Alexa Fluor 594-conjugated donkey anti-goat IgG (#A11058, Thermo Fisher Scientific, 1:200), and Alexa Fluor 488-conjugated donkey anti-chicken IgY (#703-545-155, Jackson ImmunoResearch, 1:200).

Article Title: Volume gradients in inner hair cell-auditory nerve fiber pre- and postsynaptic proteins differ across mouse strains.
Article Snippet: .. Structure Protein Antibody type Isotype Origin Concentration Primary antibodies Presynaptic ribbon protein (also labels the inner hair cell nucleus) CTBP2 mouse monoclonal IgG1 BD Biosciences cat. no. 612044 0.83 μg/ml (1:300) Postsynaptic Glutamate receptor GluA2 mouse monoclonal IgG2a Millipore cat. no. MAB 397 7.1 μg/ml (1:300) Postsynaptic density scaffold protein Shank1a C-terminus rabbit polyclonal Neuromics cat. no. RA19016 3.4 μg/ml (1:300) Postsynaptic density scaffold protein Homer1/2/3 rabbit polyclonal Synaptic Systems cat. no. 160 103 3.33 μg/ml (1:300) Postsynaptic density scaffold protein PSD95 mouse monoclonal IgG2a Neuromab cat. no. 75-028 3.43 μg/ml (1:300) Inner hair cell protein Myosin 7A rabbit polyclonal, Proteus Biosciences cat. no. 25-6790 1 μg/ml (1:1000)p Inner hair cell protein Myosin 7A mouse monoclonal IgG2a Santa Cruz biotech. cat. no. sc-74516 0.4 μg/ml (1:500)p Host species Target species Conjugate Isotype Origin Concentration Secondary antibodies Goat Anti mouse alexa fluor 488 IgG1 Invitrogen cat. no. A-21121 4 μg/ml (1:500) Goat Anti rabbit alexa fluor 568 Invitrogen cat. no. A-11011 4 μg/ml (1:500) Goat Anti mouse alexa fluor 647 IgG2a Invitrogen cat. no. A-21241 4 μg/ml (1:500) P = Inner hair cell labeling was only used to ascertain that the plotted plane more or less bisected the row of inner hair cells. ..

Immunohistochemistry:

Article Title: Otoferlin Is Required for Proper Synapse Maturation and for Maintenance of Inner and Outer Hair Cells in Mouse Models for DFNB9
Article Snippet: .. The following antibodies were used: mouse anti-Ctbp2 (#612044, BD Bioscience, 1:50–1:200) for labelling synaptic ribbons, rabbit anti-Na/K-ATPase (SC28800, Santa-Cruz Biotech, 1:200) or rabbit anti-Shank1a (#RA19016, Neuromics, 1:300) for labelling postsynaptic boutons , guinea pig anti-parvalbumin (#195004, Synaptic Systems, 1:200) to label IHC and OHC as well as SGN cell bodies and afferent fibers, guinea pig anti-Vglut3 (#135204, Synaptic Systems, 1:300) to label IHCs, goat anti-calretinin (CG1, Swant, 1:300) to label IHC and OHC, mouse anti-neurofilament (N5389, Sigma, 1:400) to label afferent fibers and SGN cell bodies, mouse anti-MyoVI (#H00004646-M02, Novus Biological, 1:200) or rabbit anti-MyoVI (#25-6791, Proteus, 1:200) antibodies for labelling hair cells, and rabbit anti-oncomodulin (OMG4, Swant, 1:300–1:1,000) for labelling OHC. .. The following secondary antibodies were used: DyLight 405 donkey anti-guinea pig (Jackson Immuno, 1:200), Alexa Fluor 568 IgG H + L goat anti-mouse, Alexa Fluor 633 IgG goat anti-rabbit (MoBiTec, 1:200), Alexa Fluor 488 goat anti-guinea pig, Alexa Fluor 488 goat anti-mouse IgG, IgA IgM (H + L), Alexa Fluor 647 donkey anti-rabbit, Alexa Fluor 568 donkey anti-goat, Alexa Fluor 488 donkey anti-mouse (Invitrogen, 1:200).

Article Title: Otoferlin Is Required for Proper Synapse Maturation and for Maintenance of Inner and Outer Hair Cells in Mouse Models for DFNB9.
Article Snippet: .. The following antibodies were used: mouse anti-Ctbp2 (#612044, BD Bioscience, 1:50–1:200) for labelling synaptic ribbons, rabbit anti-Na/K-ATPase (SC28800, Santa-Cruz Biotech, 1:200) or rabbit anti-Shank1a (#RA19016, Neuromics, 1:300) for labelling postsynaptic boutons (Huang et al., 2012), guinea pig anti-parvalbumin (#195004, Synaptic Systems, 1:200) to label IHC and OHC as well as SGN cell bodies and afferent fibers, guinea pig anti-Vglut3 (#135204, Synaptic Systems, 1:300) to label IHCs, goat anti-calretinin (CG1, Swant, 1:300) to label IHC and OHC, mouse anti-neurofilament (N5389, Sigma, 1:400) to label afferent fibers and SGN cell bodies, mouse antiMyoVI (#H00004646-M02, Novus Biological, 1:200) or rabbit anti-MyoVI (#25-6791, Proteus, 1:200) antibodies for labelling hair cells, and rabbit anti-oncomodulin (OMG4, Swant, 1:300– 1:1,000) for labelling OHC. .. The following secondary antibodies were used: DyLight 405 donkey anti-guinea pig (Jackson Immuno, 1:200), Alexa Fluor 568 IgG H + L goat anti-mouse, Alexa Fluor 633 IgG goat anti-rabbit (MoBiTec, 1:200), Alexa Fluor 488 goat anti-guinea pig, Alexa Fluor 488 goat anti-mouse IgG, IgA IgM (H + L), Alexa Fluor 647 donkey anti-rabbit, Alexa Fluor 568 donkey antigoat, Alexa Fluor 488 donkey anti-mouse (Invitrogen, 1:200).

Concentration Assay:

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anti c terminus antibody (Pacific Immunology)

Pacific Immunology anti c terminus antibody
$The cleaved form of <t>the</t> <t>Cav1.3-C-terminus</t> is localized within the nucleus. ( A ) Schematic representation of the Cav1.3 α1 subunit with the four domains (I–IV), six repeat segments (1–6), pore region (P), N-terminus (brown), intracellular loops (IC, green) and the C-terminus (C-term, blue) with the relevant regulatory domains (Isoleucine-glutamine (IQ); EF-Helix binding motif (EF hand)). The commercial anti-II-III loop and <t>anti-C-terminus</t> antibodies’ epitope are shown in red. Anti-Ca v 1.3-C-terminus antibody labeling shows green fluorescent staining of the nucleus and the cytoplasm in ( B ) non-infected neonatal rat myocytes (NRVMs), ( E ) non-infected adult rat atrial myocytes (ARAMs) and ( H ) Ca v 1.3-transfected tsA201 cells. DAPI in blue ( C , F ) and red ( I ) is a nuclear staining marker. Arrows indicate nuclear staining. Overlay images are shown in ( D , G , J ). The scale bar is 20 μm. ( K ) Surface plot illustrating the staining intensity of the C-terminus in the nucleus and cytoplasm using the anti-C-terminus antibody. The central gray peak corresponds to nuclear green fluorescence. ( L ) Representative Western blots of nuclear and cyptoplasmic fractions from non-infected NRVMs and ARAMs probed with the C-terminus antibody (n = 5). Histone Deacetylase (HDAC) and Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) were used as internal controls.$
Anti C Terminus Antibody, supplied by Pacific Immunology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+terminus/anti+c+terminus/pmc13162661-115-16-18
Average 86 stars, based on 1 article reviews

anti c terminus antibody - by Bioz Stars, 2026-11

86/100 stars

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myeloperoxidase mpo (Boster Bio)

Boster Bio myeloperoxidase mpo
$The cleaved form of <t>the</t> <t>Cav1.3-C-terminus</t> is localized within the nucleus. ( A ) Schematic representation of the Cav1.3 α1 subunit with the four domains (I–IV), six repeat segments (1–6), pore region (P), N-terminus (brown), intracellular loops (IC, green) and the C-terminus (C-term, blue) with the relevant regulatory domains (Isoleucine-glutamine (IQ); EF-Helix binding motif (EF hand)). The commercial anti-II-III loop and <t>anti-C-terminus</t> antibodies’ epitope are shown in red. Anti-Ca v 1.3-C-terminus antibody labeling shows green fluorescent staining of the nucleus and the cytoplasm in ( B ) non-infected neonatal rat myocytes (NRVMs), ( E ) non-infected adult rat atrial myocytes (ARAMs) and ( H ) Ca v 1.3-transfected tsA201 cells. DAPI in blue ( C , F ) and red ( I ) is a nuclear staining marker. Arrows indicate nuclear staining. Overlay images are shown in ( D , G , J ). The scale bar is 20 μm. ( K ) Surface plot illustrating the staining intensity of the C-terminus in the nucleus and cytoplasm using the anti-C-terminus antibody. The central gray peak corresponds to nuclear green fluorescence. ( L ) Representative Western blots of nuclear and cyptoplasmic fractions from non-infected NRVMs and ARAMs probed with the C-terminus antibody (n = 5). Histone Deacetylase (HDAC) and Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) were used as internal controls.$
Myeloperoxidase Mpo, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+terminus/Anti-HDAC-2+(C-terminus)+Antibody/pmc13131401-117-14-36
Average 93 stars, based on 1 article reviews

myeloperoxidase mpo - by Bioz Stars, 2026-11

93/100 stars

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histone deacetylase 2 (Boster Bio)

Boster Bio histone deacetylase 2
$The cleaved form of <t>the</t> <t>Cav1.3-C-terminus</t> is localized within the nucleus. ( A ) Schematic representation of the Cav1.3 α1 subunit with the four domains (I–IV), six repeat segments (1–6), pore region (P), N-terminus (brown), intracellular loops (IC, green) and the C-terminus (C-term, blue) with the relevant regulatory domains (Isoleucine-glutamine (IQ); EF-Helix binding motif (EF hand)). The commercial anti-II-III loop and <t>anti-C-terminus</t> antibodies’ epitope are shown in red. Anti-Ca v 1.3-C-terminus antibody labeling shows green fluorescent staining of the nucleus and the cytoplasm in ( B ) non-infected neonatal rat myocytes (NRVMs), ( E ) non-infected adult rat atrial myocytes (ARAMs) and ( H ) Ca v 1.3-transfected tsA201 cells. DAPI in blue ( C , F ) and red ( I ) is a nuclear staining marker. Arrows indicate nuclear staining. Overlay images are shown in ( D , G , J ). The scale bar is 20 μm. ( K ) Surface plot illustrating the staining intensity of the C-terminus in the nucleus and cytoplasm using the anti-C-terminus antibody. The central gray peak corresponds to nuclear green fluorescence. ( L ) Representative Western blots of nuclear and cyptoplasmic fractions from non-infected NRVMs and ARAMs probed with the C-terminus antibody (n = 5). Histone Deacetylase (HDAC) and Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) were used as internal controls.$
Histone Deacetylase 2, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+terminus/Anti-HDAC-2+(C-terminus)+Antibody/pmc13131401-117-7-36
Average 93 stars, based on 1 article reviews

histone deacetylase 2 - by Bioz Stars, 2026-11

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interleukin 6 (Boster Bio)

Boster Bio interleukin 6
$The cleaved form of <t>the</t> <t>Cav1.3-C-terminus</t> is localized within the nucleus. ( A ) Schematic representation of the Cav1.3 α1 subunit with the four domains (I–IV), six repeat segments (1–6), pore region (P), N-terminus (brown), intracellular loops (IC, green) and the C-terminus (C-term, blue) with the relevant regulatory domains (Isoleucine-glutamine (IQ); EF-Helix binding motif (EF hand)). The commercial anti-II-III loop and <t>anti-C-terminus</t> antibodies’ epitope are shown in red. Anti-Ca v 1.3-C-terminus antibody labeling shows green fluorescent staining of the nucleus and the cytoplasm in ( B ) non-infected neonatal rat myocytes (NRVMs), ( E ) non-infected adult rat atrial myocytes (ARAMs) and ( H ) Ca v 1.3-transfected tsA201 cells. DAPI in blue ( C , F ) and red ( I ) is a nuclear staining marker. Arrows indicate nuclear staining. Overlay images are shown in ( D , G , J ). The scale bar is 20 μm. ( K ) Surface plot illustrating the staining intensity of the C-terminus in the nucleus and cytoplasm using the anti-C-terminus antibody. The central gray peak corresponds to nuclear green fluorescence. ( L ) Representative Western blots of nuclear and cyptoplasmic fractions from non-infected NRVMs and ARAMs probed with the C-terminus antibody (n = 5). Histone Deacetylase (HDAC) and Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) were used as internal controls.$
Interleukin 6, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+terminus/Anti-HDAC-2+(C-terminus)+Antibody/pmc13131401-117-5-36
Average 93 stars, based on 1 article reviews

interleukin 6 - by Bioz Stars, 2026-11

93/100 stars

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gp antibodies successfully detected trpv1 (Neuromics)

Neuromics gp antibodies successfully detected trpv1

Calcium imaging and immunocytochemistry showing <t>TRPV1</t> expression in transfected HEK‐293 cells. (A) Fura2 calcium imaging in HEK‐293 cells transiently expressing rTRPV1‐EYFP fusion protein. Representative traces of calcium transients evoked by capsaicin in EYFP + cells. Green traces correspond to EYFP + cells and gray traces to EYFP − cells. Pie chart represents the percentage of EYFP + cells that responded or failded to respond to capsaicin. Data obtained from 88 cells from 3 coverslips. (B–F left) Confocal images of TRPV1‐EYFP transiently expressed in HEK‐293 cells. EYFP (green), TRPV1 antibody (magenta) and Hoechst (HO, blue). Merge images correspond to the overlap between TRPV1 and EYFP on the left and Hoechst and brightfield (BF) on the right. Antibody dilution of the representative images is indicated in the lower right corner. Scale bar: 20 μm. (B–F right) Box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single EYFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (*** p < 0.001 Mann–Whitney test). (G) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit, goat or guinea pig) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 100 cells were analyzed across 4 fields from 2 independent transfections. $ Santa Cruz V1 antibody was generated against a human TRPA1 peptide.

Gp Antibodies Successfully Detected Trpv1, supplied by Neuromics, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+terminus/VR1+C-terminus+(TRPV1)/pmc13100940-193-5-4
Average 93 stars, based on 1 article reviews

gp antibodies successfully detected trpv1 - by Bioz Stars, 2026-11

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rabbit polyclonal anti-aph1a picoband® against the c-terminus of human aph1a (Boster Bio)

Boster Bio rabbit polyclonal anti-aph1a picoband® against the c-terminus of human aph1a

Rabbit Polyclonal Anti Aph1a Picoband® Against The C Terminus Of Human Aph1a, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+terminus/Anti-APH1a+Antibody+Picoband/pmc13130637-9-0-15
Average 94 stars, based on 1 article reviews

rabbit polyclonal anti-aph1a picoband® against the c-terminus of human aph1a - by Bioz Stars, 2026-11

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HDAC2 enhances antimicrobial activities against E.coli in mice. (A) Bacterial loads in the blood were calculated in HDAC2-knockout and wild type mice. (A)The picture of the bacterial clones was taken by BIO-RAD ChemiDoc“MP Imaging System. (B)The number of colony-forming units (CFU) in the blood of mice was calculated. HDAC2 WT and HDAC2 KO mice were administrated with E. coli- GFP (5 × 10 7 ) by intravenous Injection. Blood (20 μl) was taken from the tail vein of the mice, applied to the pretreated solid MHA medium, and incubated at 37 °C for 20 h. Count the colonies on the culture medium and take pictures. The experiments were performed in quintuplicate, data are presented as the means ± SEM. of independent experiments. *P < 0.05, **P < 0.01 (two-tailed Student’s t -test). (B) Bacterial loads in the kidneys were calculated in HDAC2-knockout and wild type mice. The picture of kidneys from HDAC2 WT and HDAC2 KO mice were taken. The number of colony-forming units (CFU) in the kidneys of mice was calculated. The kidneys from HDAC2 KO and HDAC2 WT mice were weighed, ground, spread on the MHA plates, incubated for 16–24 h at 37 °C, and then bacterial clones were counted. The experiments were performed in quintuplicate, data are presented as the means ± SEM. of independent experiments. *P < 0.05, **P < 0.01 (two-tailed Student’s t -test). n = 5 mice per group. (C) H&E staining of livers and kidneys. The arrows indicate damage section of tissue. (D) Serum CRP and PCT levels of mice with E. coli- GFP bacteremia at 24 h. The CRP and PCT were measured by ELISA. Data are shown as mean ± SEM. *p < 0.05; n = 5 mice per group. ns, not significant.

Journal: Journal of Advanced Research

Article Title: HDAC2 enhances the antimicrobial activity of neutrophils by promoting the formation of neutrophil extracellular traps (NETs) in sepsis

doi: 10.1016/j.jare.2025.08.041

Figure Lengend Snippet: HDAC2 enhances antimicrobial activities against E.coli in mice. (A) Bacterial loads in the blood were calculated in HDAC2-knockout and wild type mice. (A)The picture of the bacterial clones was taken by BIO-RAD ChemiDoc“MP Imaging System. (B)The number of colony-forming units (CFU) in the blood of mice was calculated. HDAC2 WT and HDAC2 KO mice were administrated with E. coli- GFP (5 × 10 7 ) by intravenous Injection. Blood (20 μl) was taken from the tail vein of the mice, applied to the pretreated solid MHA medium, and incubated at 37 °C for 20 h. Count the colonies on the culture medium and take pictures. The experiments were performed in quintuplicate, data are presented as the means ± SEM. of independent experiments. *P < 0.05, **P < 0.01 (two-tailed Student’s t -test). (B) Bacterial loads in the kidneys were calculated in HDAC2-knockout and wild type mice. The picture of kidneys from HDAC2 WT and HDAC2 KO mice were taken. The number of colony-forming units (CFU) in the kidneys of mice was calculated. The kidneys from HDAC2 KO and HDAC2 WT mice were weighed, ground, spread on the MHA plates, incubated for 16–24 h at 37 °C, and then bacterial clones were counted. The experiments were performed in quintuplicate, data are presented as the means ± SEM. of independent experiments. *P < 0.05, **P < 0.01 (two-tailed Student’s t -test). n = 5 mice per group. (C) H&E staining of livers and kidneys. The arrows indicate damage section of tissue. (D) Serum CRP and PCT levels of mice with E. coli- GFP bacteremia at 24 h. The CRP and PCT were measured by ELISA. Data are shown as mean ± SEM. *p < 0.05; n = 5 mice per group. ns, not significant.

Article Snippet: Concentrations of interleukin-1 beta (IL-1β), interleukin-6 (IL-6), histone deacetylase 2 (HDAC2), PCT, CRP and myeloperoxidase (MPO) in mouse serum were quantified using their respective enzyme-linked immunosorbent assay (ELISA) kits, following the protocols provided by the manufacturer (Boster Biological Technology, Wuhan, China).

Techniques: Knock-Out, Clone Assay, Imaging, Injection, Incubation, Two Tailed Test, Staining, Enzyme-linked Immunosorbent Assay

$The cleaved form of the Cav1.3-C-terminus is localized within the nucleus. ( A ) Schematic representation of the Cav1.3 α1 subunit with the four domains (I–IV), six repeat segments (1–6), pore region (P), N-terminus (brown), intracellular loops (IC, green) and the C-terminus (C-term, blue) with the relevant regulatory domains (Isoleucine-glutamine (IQ); EF-Helix binding motif (EF hand)). The commercial anti-II-III loop and anti-C-terminus antibodies’ epitope are shown in red. Anti-Ca v 1.3-C-terminus antibody labeling shows green fluorescent staining of the nucleus and the cytoplasm in ( B ) non-infected neonatal rat myocytes (NRVMs), ( E ) non-infected adult rat atrial myocytes (ARAMs) and ( H ) Ca v 1.3-transfected tsA201 cells. DAPI in blue ( C , F ) and red ( I ) is a nuclear staining marker. Arrows indicate nuclear staining. Overlay images are shown in ( D , G , J ). The scale bar is 20 μm. ( K ) Surface plot illustrating the staining intensity of the C-terminus in the nucleus and cytoplasm using the anti-C-terminus antibody. The central gray peak corresponds to nuclear green fluorescence. ( L ) Representative Western blots of nuclear and cyptoplasmic fractions from non-infected NRVMs and ARAMs probed with the C-terminus antibody (n = 5). Histone Deacetylase (HDAC) and Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) were used as internal controls.$

Journal: Cells

Article Title: C-Terminus of Ca v 1.3 L-Type Ca 2+ Channel Upregulates Its Own Gene Expression

doi: 10.3390/cells15090828

Figure Lengend Snippet: The cleaved form of the Cav1.3-C-terminus is localized within the nucleus. ( A ) Schematic representation of the Cav1.3 α1 subunit with the four domains (I–IV), six repeat segments (1–6), pore region (P), N-terminus (brown), intracellular loops (IC, green) and the C-terminus (C-term, blue) with the relevant regulatory domains (Isoleucine-glutamine (IQ); EF-Helix binding motif (EF hand)). The commercial anti-II-III loop and anti-C-terminus antibodies’ epitope are shown in red. Anti-Ca v 1.3-C-terminus antibody labeling shows green fluorescent staining of the nucleus and the cytoplasm in ( B ) non-infected neonatal rat myocytes (NRVMs), ( E ) non-infected adult rat atrial myocytes (ARAMs) and ( H ) Ca v 1.3-transfected tsA201 cells. DAPI in blue ( C , F ) and red ( I ) is a nuclear staining marker. Arrows indicate nuclear staining. Overlay images are shown in ( D , G , J ). The scale bar is 20 μm. ( K ) Surface plot illustrating the staining intensity of the C-terminus in the nucleus and cytoplasm using the anti-C-terminus antibody. The central gray peak corresponds to nuclear green fluorescence. ( L ) Representative Western blots of nuclear and cyptoplasmic fractions from non-infected NRVMs and ARAMs probed with the C-terminus antibody (n = 5). Histone Deacetylase (HDAC) and Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) were used as internal controls.

Article Snippet: Briefly, cardiac cells were fixed, permeabilized, blocked, and incubated overnight at 4 °C with a primary anti-C-terminus antibody (Pacific Immunology, Ramona, CA, USA) targeting a unique epitope (amino acids 1708–1724) in the C-terminus of Ca v 1.3 and detected with FITC-conjugated anti-rabbit antibody (1:200).

Techniques: Binding Assay, Antibody Labeling, Staining, Infection, Transfection, Marker, Fluorescence, Western Blot, Histone Deacetylase Assay

Calcium imaging and immunocytochemistry showing TRPV1 expression in transfected HEK‐293 cells. (A) Fura2 calcium imaging in HEK‐293 cells transiently expressing rTRPV1‐EYFP fusion protein. Representative traces of calcium transients evoked by capsaicin in EYFP + cells. Green traces correspond to EYFP + cells and gray traces to EYFP − cells. Pie chart represents the percentage of EYFP + cells that responded or failded to respond to capsaicin. Data obtained from 88 cells from 3 coverslips. (B–F left) Confocal images of TRPV1‐EYFP transiently expressed in HEK‐293 cells. EYFP (green), TRPV1 antibody (magenta) and Hoechst (HO, blue). Merge images correspond to the overlap between TRPV1 and EYFP on the left and Hoechst and brightfield (BF) on the right. Antibody dilution of the representative images is indicated in the lower right corner. Scale bar: 20 μm. (B–F right) Box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single EYFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (*** p < 0.001 Mann–Whitney test). (G) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit, goat or guinea pig) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 100 cells were analyzed across 4 fields from 2 independent transfections. $ Santa Cruz V1 antibody was generated against a human TRPA1 peptide.

Journal: Journal of Neurochemistry

Article Title: Validation of TRPA1 and TRPV1 Antibodies for Expression Detection in Mammalian Cells and Tissues

doi: 10.1111/jnc.70444

Figure Lengend Snippet: Calcium imaging and immunocytochemistry showing TRPV1 expression in transfected HEK‐293 cells. (A) Fura2 calcium imaging in HEK‐293 cells transiently expressing rTRPV1‐EYFP fusion protein. Representative traces of calcium transients evoked by capsaicin in EYFP + cells. Green traces correspond to EYFP + cells and gray traces to EYFP − cells. Pie chart represents the percentage of EYFP + cells that responded or failded to respond to capsaicin. Data obtained from 88 cells from 3 coverslips. (B–F left) Confocal images of TRPV1‐EYFP transiently expressed in HEK‐293 cells. EYFP (green), TRPV1 antibody (magenta) and Hoechst (HO, blue). Merge images correspond to the overlap between TRPV1 and EYFP on the left and Hoechst and brightfield (BF) on the right. Antibody dilution of the representative images is indicated in the lower right corner. Scale bar: 20 μm. (B–F right) Box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single EYFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (*** p < 0.001 Mann–Whitney test). (G) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit, goat or guinea pig) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 100 cells were analyzed across 4 fields from 2 independent transfections. $ Santa Cruz V1 antibody was generated against a human TRPA1 peptide.

Article Snippet: Only the Abcam and Neuromics GP antibodies successfully detected TRPV1 (Figure , full uncropped blots in Figure ), consistent with their superior efficiency in ICC of TRPV1‐transfected cells (Figure ).

Techniques: Imaging, Immunocytochemistry, Expressing, Transfection, MANN-WHITNEY, Generated

Western blot analysis for TRPV1 and TRPA1 antibodies specificity. (A) Immunoblots for TRPV1 using Abcam (1:1000), Millipore V1 (1:1000), Santa Cruz V1 (1:100), Neuromics GT (1:200), and Neuromics GP (1:1000) antibodies. (−) lanes: Untransfected HEK‐293 cells, (+) lanes: HEK‐293 cells transfected with rTRPV1‐EYFP (B) Immunoblots for TRPA1 using Aviva (1:300), Novus (1:500), Millipore A1 (1:500), Alomone (1:200), Proteintech (1:500), Sigma WH (1:500), and Santa Cruz A1 (1:100). (−) lanes: Untransfected HEK‐293 cells, (+) lanes: HEK‐293 cells transfected with hTRPA1‐tGFFP. For each antibody, top row: Immunoblot with each TRPV1/TRPA1 antibody. Middle row: EYFP/tGFP immunoblotting of the same membrane. Bottom image: GAPDH loading control. Each blot was performed at least three times to rule out technical artifacts in cases where no anti‐TRPV1/TRPA1 signal was detected. For every repetition, the identical lysate sample was probed with all antibodies. Full uncropped blots are available in the Figure . $ Santa Cruz V1 antibody was generated against a human TRPA1 peptide.

Journal: Journal of Neurochemistry

Article Title: Validation of TRPA1 and TRPV1 Antibodies for Expression Detection in Mammalian Cells and Tissues

doi: 10.1111/jnc.70444

Figure Lengend Snippet: Western blot analysis for TRPV1 and TRPA1 antibodies specificity. (A) Immunoblots for TRPV1 using Abcam (1:1000), Millipore V1 (1:1000), Santa Cruz V1 (1:100), Neuromics GT (1:200), and Neuromics GP (1:1000) antibodies. (−) lanes: Untransfected HEK‐293 cells, (+) lanes: HEK‐293 cells transfected with rTRPV1‐EYFP (B) Immunoblots for TRPA1 using Aviva (1:300), Novus (1:500), Millipore A1 (1:500), Alomone (1:200), Proteintech (1:500), Sigma WH (1:500), and Santa Cruz A1 (1:100). (−) lanes: Untransfected HEK‐293 cells, (+) lanes: HEK‐293 cells transfected with hTRPA1‐tGFFP. For each antibody, top row: Immunoblot with each TRPV1/TRPA1 antibody. Middle row: EYFP/tGFP immunoblotting of the same membrane. Bottom image: GAPDH loading control. Each blot was performed at least three times to rule out technical artifacts in cases where no anti‐TRPV1/TRPA1 signal was detected. For every repetition, the identical lysate sample was probed with all antibodies. Full uncropped blots are available in the Figure . $ Santa Cruz V1 antibody was generated against a human TRPA1 peptide.

Techniques: Western Blot, Transfection, Membrane, Control, Generated

Immunocytochemistry of endogenously expressed TRPV1 in DRG cultures from rTRPV1‐EGFP transgenic mice. (A–D left): Confocal images of TRPV1 + DRG neurons incubated with the indicated antibody. EGFP (green), TRPV1 antibody (magenta) and βIII‐Tubulin (cyan). Scale bar: 50 μm. (A–D right) box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single EGFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (*** p < 0.001 Mann–Whitney test). (E) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit, goat or guinea pig) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 92 cells were analyzed across 4 microscopic fields from 2 different animals.

Journal: Journal of Neurochemistry

Article Title: Validation of TRPA1 and TRPV1 Antibodies for Expression Detection in Mammalian Cells and Tissues

doi: 10.1111/jnc.70444

Figure Lengend Snippet: Immunocytochemistry of endogenously expressed TRPV1 in DRG cultures from rTRPV1‐EGFP transgenic mice. (A–D left): Confocal images of TRPV1 + DRG neurons incubated with the indicated antibody. EGFP (green), TRPV1 antibody (magenta) and βIII‐Tubulin (cyan). Scale bar: 50 μm. (A–D right) box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single EGFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (*** p < 0.001 Mann–Whitney test). (E) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit, goat or guinea pig) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 92 cells were analyzed across 4 microscopic fields from 2 different animals.

Techniques: Immunocytochemistry, Transgenic Assay, Incubation, MANN-WHITNEY

Immunohistochemistry of endogenously expressed TRPV1 in DRG slices from rTRPV1‐ EGFP transgenic mice. (A–D left): Confocal images of TRPV1 + DRG neurons incubated with the indicated antibody. EGFP (green), TRPV1 antibody (magenta) and βIII‐Tubulin (cyan). Scale bar: 50 μm. (A–D right) box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single EGFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (* p < 0.05, *** p < 0.001 Mann–Whitney test). (E) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit, goat or guinea pig) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 141 cells were analyzed across 4 pictures from 2 different animals.

Journal: Journal of Neurochemistry

Article Title: Validation of TRPA1 and TRPV1 Antibodies for Expression Detection in Mammalian Cells and Tissues

doi: 10.1111/jnc.70444

Figure Lengend Snippet: Immunohistochemistry of endogenously expressed TRPV1 in DRG slices from rTRPV1‐ EGFP transgenic mice. (A–D left): Confocal images of TRPV1 + DRG neurons incubated with the indicated antibody. EGFP (green), TRPV1 antibody (magenta) and βIII‐Tubulin (cyan). Scale bar: 50 μm. (A–D right) box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single EGFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (* p < 0.05, *** p < 0.001 Mann–Whitney test). (E) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit, goat or guinea pig) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 141 cells were analyzed across 4 pictures from 2 different animals.

Techniques: Immunohistochemistry, Transgenic Assay, Incubation, MANN-WHITNEY

Comparison of immunofluorescence of endogenously expressed TRPV1 in cultured cells and slices from TRPV1‐KO (KO) and TRPV1‐EGFP mice. (A–C) Immunocytochemistry. (D–F) Immunohistochemistry. (A–) Confocal images of cultured DRG neurons and DRG tissue sections (D–F) from TRPV1‐KO (KO, left) and TRPV1‐EGFP (right). EGFP (green), TRPV1 antibody (magenta), and βIII‐Tubulin (cyan). Scale bar: 50 μm. (A–F) For each antibody and dilution, 4 pictures from 2 different animals were studied, image display settings are the same for KO and TRPV1‐EGFP.

Journal: Journal of Neurochemistry

Article Title: Validation of TRPA1 and TRPV1 Antibodies for Expression Detection in Mammalian Cells and Tissues

doi: 10.1111/jnc.70444

Figure Lengend Snippet: Comparison of immunofluorescence of endogenously expressed TRPV1 in cultured cells and slices from TRPV1‐KO (KO) and TRPV1‐EGFP mice. (A–C) Immunocytochemistry. (D–F) Immunohistochemistry. (A–) Confocal images of cultured DRG neurons and DRG tissue sections (D–F) from TRPV1‐KO (KO, left) and TRPV1‐EGFP (right). EGFP (green), TRPV1 antibody (magenta), and βIII‐Tubulin (cyan). Scale bar: 50 μm. (A–F) For each antibody and dilution, 4 pictures from 2 different animals were studied, image display settings are the same for KO and TRPV1‐EGFP.

Techniques: Comparison, Immunofluorescence, Cell Culture, Immunocytochemistry, Immunohistochemistry

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1) Product Images from "Association of Shank 1A Scaffolding Protein with Cone Photoreceptor Terminals in the Mammalian Retina"

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