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part1-wt  (Promega)

 
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    Promega part1-wt
    The sequence of reagents used in cell transfection
    Part1 Wt, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+socket+programming+tutorial+part+1/pmc08430160-181-9-19?v=Promega
    Average 90 stars, based on 1 article reviews
    part1-wt - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "lncRNA PART1 mitigates MPP + -induced neuronal injury in SH-SY5Y cells via micRNA-106b-5p/MCL1 axis"

    Article Title: lncRNA PART1 mitigates MPP + -induced neuronal injury in SH-SY5Y cells via micRNA-106b-5p/MCL1 axis

    Journal: American Journal of Translational Research

    doi:

    The sequence of reagents used in cell transfection
    Figure Legend Snippet: The sequence of reagents used in cell transfection

    Techniques Used: Sequencing

    The sequence of primers
    Figure Legend Snippet: The sequence of primers

    Techniques Used: Sequencing

    The expression of PART1 in PD. A: The protein expressions of TH and α-synuclein were examined by western blot in the mouse model of PD. β-actin was used as lading control; B: The expression of PART1 in brain tissues was examined in the mouse model of PD by RT-qPCR; C: The expression of PART1 in SH-SY5Y cells treated with MPP+ was examined by RT-qPCR. **P<0.01. PD: Parkinson’s disease; MPP+: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.
    Figure Legend Snippet: The expression of PART1 in PD. A: The protein expressions of TH and α-synuclein were examined by western blot in the mouse model of PD. β-actin was used as lading control; B: The expression of PART1 in brain tissues was examined in the mouse model of PD by RT-qPCR; C: The expression of PART1 in SH-SY5Y cells treated with MPP+ was examined by RT-qPCR. **P<0.01. PD: Parkinson’s disease; MPP+: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.

    Techniques Used: Expressing, Western Blot, Quantitative RT-PCR

    Overexpression of PART1 alleviated neuronal injury. SH-SY5Y cells transfected with pcDNA3.1-NC or PART1 pcDNA3.1 were treated with 1 μM MPP+ for 48 h. A: The expression of PART1 was examined by RT-qPCR; B: The cell viability was assessed by CCK8 assay; C: The cell apoptosis rate was detected by Annexin V-FITC/PI assay; D: The expression of cleaved caspase 3 was detected by western blot; E: The activity of cleaved caspase 3 was detected; F-H: The levels of inflammatory cytokines were examined by ELISA; I-K: The levels of LDH, ROS and SOD were examined. *P<0.05, **P<0.01. MPP+: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; LDH: Lactate dehydrogenase; ROS: Reactive oxygen; SOD: Superoxide dismutase.
    Figure Legend Snippet: Overexpression of PART1 alleviated neuronal injury. SH-SY5Y cells transfected with pcDNA3.1-NC or PART1 pcDNA3.1 were treated with 1 μM MPP+ for 48 h. A: The expression of PART1 was examined by RT-qPCR; B: The cell viability was assessed by CCK8 assay; C: The cell apoptosis rate was detected by Annexin V-FITC/PI assay; D: The expression of cleaved caspase 3 was detected by western blot; E: The activity of cleaved caspase 3 was detected; F-H: The levels of inflammatory cytokines were examined by ELISA; I-K: The levels of LDH, ROS and SOD were examined. *P<0.05, **P<0.01. MPP+: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; LDH: Lactate dehydrogenase; ROS: Reactive oxygen; SOD: Superoxide dismutase.

    Techniques Used: Over Expression, Transfection, Expressing, Quantitative RT-PCR, CCK-8 Assay, Western Blot, Activity Assay, Enzyme-linked Immunosorbent Assay

    PART1 targets microRNA-106b-5p directly. (A) The predictive binding site of PART1 was predicted by Starbase 2.0; (B) The expression of microRNA-106b-5p was examined by RT-qPCR in SH-SY5Y cells transfected with PART1 pcDNA3.1; (C) The expression of microRNA-106b-5p was examined by RT-qPCR in the SH-SY5Y cells transfected with mimics NC and microRNA-106b-5p mimics; (D) The DLR assay was used to detect the binding relationship between microRNA-106b-5p and PART1 in SH-SY5Y cells co-transfected with PART1-WT or PART1-MUT and mimics NC or microRNA-106b-5p mimics; (E, F) The RIP assay (E) and RNA pull down assay (F) were used to examine the interaction between PART1 and microRNA-106b-5p in SH-SY5Y cells; (G, H) The expressions of microRNA-106b-5p in SH-SY5Y cells (G) and PD mouse model (H) were examined by RT-qPCR; (I) The correlation analysis of PART1 and microRNA-106b-5p in PD mouse model. **P<0.01. DLR: Dual-luciferase report; PD: Parkinson’s disease.
    Figure Legend Snippet: PART1 targets microRNA-106b-5p directly. (A) The predictive binding site of PART1 was predicted by Starbase 2.0; (B) The expression of microRNA-106b-5p was examined by RT-qPCR in SH-SY5Y cells transfected with PART1 pcDNA3.1; (C) The expression of microRNA-106b-5p was examined by RT-qPCR in the SH-SY5Y cells transfected with mimics NC and microRNA-106b-5p mimics; (D) The DLR assay was used to detect the binding relationship between microRNA-106b-5p and PART1 in SH-SY5Y cells co-transfected with PART1-WT or PART1-MUT and mimics NC or microRNA-106b-5p mimics; (E, F) The RIP assay (E) and RNA pull down assay (F) were used to examine the interaction between PART1 and microRNA-106b-5p in SH-SY5Y cells; (G, H) The expressions of microRNA-106b-5p in SH-SY5Y cells (G) and PD mouse model (H) were examined by RT-qPCR; (I) The correlation analysis of PART1 and microRNA-106b-5p in PD mouse model. **P<0.01. DLR: Dual-luciferase report; PD: Parkinson’s disease.

    Techniques Used: Binding Assay, Expressing, Quantitative RT-PCR, Transfection, Pull Down Assay, Luciferase

    PART1 attenuated the injury of SH-SY5Y cells induced by MPP+ via microRNA-106b-5p/MCL1 axis. After transfection with PART1 pcDNA3.1, microRNA-106b-5p mimics, and MCL1 siRNA, SH-SY5Y cells were treated with 1 μM MPP+ for 48 h. A: The expression of MCL1 was examined by western blot; B: The cell viability was assessed by CCK8 assay; C: The cell apoptosis rate was detected by Annexin V-FITC/PI assay; D: The expression of cleaved caspase 3 was detected by western blot; E: The activity of cleaved caspase 3 was detected; F-H: The levels of inflammatory cytokines were examined by ELISA; I-K: The levels of LDH, ROS and SOD were examined. *P<0.05, **P<0.01. MPP+: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.
    Figure Legend Snippet: PART1 attenuated the injury of SH-SY5Y cells induced by MPP+ via microRNA-106b-5p/MCL1 axis. After transfection with PART1 pcDNA3.1, microRNA-106b-5p mimics, and MCL1 siRNA, SH-SY5Y cells were treated with 1 μM MPP+ for 48 h. A: The expression of MCL1 was examined by western blot; B: The cell viability was assessed by CCK8 assay; C: The cell apoptosis rate was detected by Annexin V-FITC/PI assay; D: The expression of cleaved caspase 3 was detected by western blot; E: The activity of cleaved caspase 3 was detected; F-H: The levels of inflammatory cytokines were examined by ELISA; I-K: The levels of LDH, ROS and SOD were examined. *P<0.05, **P<0.01. MPP+: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.

    Techniques Used: Transfection, Expressing, Western Blot, CCK-8 Assay, Activity Assay, Enzyme-linked Immunosorbent Assay

    The schematic diagram of PART1/microRNA-106b-5p/MCL1 axis.
    Figure Legend Snippet: The schematic diagram of PART1/microRNA-106b-5p/MCL1 axis.

    Techniques Used:



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