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hepes buffer solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hepes buffer solution
    Hepes Buffer Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/buffer+solution/HEPES%2C+1M+Solution%2C+pH+7%2E3%2C+Molecular+Biology+Grade%2C+Ultrapure/pm42325087-50-22-25
    Average 99 stars, based on 1 article reviews
    hepes buffer solution - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: CD271 Identifies a Subpopulation with Enhanced Neural-like Potential Within Wharton Jelly Derived Mesenchymal Stem/Stromal Cells.
    Article Snippet: Following PBS washing, the slices were transferred to semipermeable membranes (Millicell CM, Millipore Merck, Saint Louis, MO, USA) and placed in 6-well plates containing seeded WJ-MSC-CD271+ and WJ-MSC-CD271− cells. .. The cells and the hippocampi were cultured in DMEM (Gibco) containing 25% HBSS (Gibco), 5% human platelet lysate (PLTGold Clinical Grade, Mill Creek Life Sciences), 1 M HEPES (Gibco), 5 mg/mL glucose (Sigma-Aldrich) and an antibiotic-antimycotic solution (Genos). ..

    Incubation:

    Article Title: Metabolic rewiring at the pyruvate node contributes to severe pneumonia and attenuation of T cell responses in serotype 3 Streptococcus pneumoniae infection.
    Article Snippet: Calu-3 cells were seeded onto a 1μm pore size of permeable membrane (Genesee Scientific) in a 6-well plate and incubated for 10 days to allow the cells to become confluent on the membrane with no gaps. .. Spn strains were incubated in EMEM supplemented with 5% FBS and 10mM HEPES (Gibco) on the cells at 37 °C in a 5% CO2 and some specific O2 conditions of atmosphere for 5 h. The attached bacteria were collected with the cells in 1 ml of PBS after the culture supernatant was removed. ..

    Bacteria:

    Article Title: Metabolic rewiring at the pyruvate node contributes to severe pneumonia and attenuation of T cell responses in serotype 3 Streptococcus pneumoniae infection.
    Article Snippet: Calu-3 cells were seeded onto a 1μm pore size of permeable membrane (Genesee Scientific) in a 6-well plate and incubated for 10 days to allow the cells to become confluent on the membrane with no gaps. .. Spn strains were incubated in EMEM supplemented with 5% FBS and 10mM HEPES (Gibco) on the cells at 37 °C in a 5% CO2 and some specific O2 conditions of atmosphere for 5 h. The attached bacteria were collected with the cells in 1 ml of PBS after the culture supernatant was removed. ..

    Passaging:

    Article Title: Integrated mesenchymal and extracellular cues drive bioengineered liver tissue formation and function
    Article Snippet: .. Advanced DMEM/F12 (Gibco) supplemented with 1% (v/v) penicillin-streptomycin (Gibco), 1% (v/v) GlutaMax (Gibco), 10 mM HEPES (Gibco), was used as the basal medium to make organoid initiation medium, expansion medium (EM), differentiation medium (DM), and to wash organoids during passaging or sample collection. .. Other major materials used for organoid isolation and maintenance are as follows: type II collagenase (Gibco), dispase (Gibco), MatrigelTM (Corning), and non-attaching 24-well plates (M9312, Greiner, Merck).



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