Review




Structured Review

LifeCanvas Technologies smartclear clearing buffer type a & b
KEY RESOURCES TABLE
Smartclear Clearing Buffer Type A & B, supplied by LifeCanvas Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+a/clearing+buffer+a/pmc09210496-31-0-8
Average 90 stars, based on 1 article reviews
smartclear clearing buffer type a & b - by Bioz Stars, 2026-10
90/100 stars

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1) Product Images from "Neural circuit pathology driven by Shank3 mutation disrupts social behaviors"

Article Title: Neural circuit pathology driven by Shank3 mutation disrupts social behaviors

Journal: Cell reports

doi: 10.1016/j.celrep.2022.110906

KEY RESOURCES TABLE
Figure Legend Snippet: KEY RESOURCES TABLE

Techniques Used: Virus, Purification, Plasmid Preparation, Recombinant, Adhesive, Electron Microscopy, Software, Optogenetics, Imaging, Microscopy, Hood, Real-time Polymerase Chain Reaction, Injection, Patch Clamp

Related Articles

Incubation:

Article Title: An Open Resource: MR and light sheet microscopy stereotaxic atlas of the mouse brain
Article Snippet: .. Samples were incubated in Clearing Buffer A (LifeCanvas Technologies) overnight then actively delipidated using a LifeCanvas Technologies SmartClear II Pro device for 6 days using stochastic electrotransport . ..

Article Title: Merged Magnetic Resonance and Light Sheet Microscopy of the Whole Mouse Brain
Article Snippet: .. Samples were incubated in Clearing Buffer A (LifeCanvas Technologies) overnight then actively delipidated using a LifeCanvas Technologies SmartClear II Pro device for 6 days using stochastic electrotransport [23] .After depilation the samples were washed in PBS with 0.1% Tween20 for 1 d to remove SDS. .. For immunolabeling the samples were incubated in SmartLabel Primary Sample Buffer (LifeCanvas Technologies) overnight with an additional 5-6 h incubation with fresh buffer before primary immunolabeling in a SmartLabel device employing eFLASH [24]technology which integrates stochastic electrotransport [23] and SWITCH [25] for 14 h. The samples were then washed in PBS for 7-8h before overnight fixation in 4% paraformaldehyde followed by incubation in secondary labeling buffer at 37 C with two refreshes over the course of 7-8h before secondary labeling in the SmartLabel device.

Article Title: Topographic connectivity and cellular profiling reveal detailed input pathways and functionally distinct cell types in the subthalamic nucleus.
Article Snippet: .. Following degassing and gel polymerization at 37 C, the sections were incubated in SmartClear Clearing Buffer Type A (LifeCanvas Technologies) at 37 C and in EasyIndex (LifeCanvas Technologies) at room temperature. .. Wide-field images were obtained using an Axioscan Z1 slide scanner (Carl ZeissMicroscopy) equipped with a 203 0.8 NAPlan-Apochromat air lens.

Article Title: Efficient 3D imaging and pathological analysis of the human lymphoma tumor microenvironment using light-sheet immunofluorescence microscopy
Article Snippet: The samples were incubated in fresh SHIELD OFF solution at 4 °C with shaking for 2 d. In a 2 mL Eppendorf tube, SHIELD-ON buffer and SHIELD-epoxy solution were mixed at a ratio of 7:1, and the sample was incubated at 37 °C with shaking for 3 h. The samples were then transferred to a new 2 mL Eppendorf tube with an equal volume of fresh SHIELD-ON buffer and incubated at 37 °C with shaking overnight. .. Then, the samples were incubated in LifeCanvas Passive Clearing Buffer at 45 °C with shaking for 3 d. After clearing was completed, the samples were incubated in PBST (1% Triton X-100 in PBS) overnight at 37 °C to wash out any remaining LifeCanvas Passive Clearing Buffer. ..

Article Title: The Duke Mouse Brain Atlas: MRI and light sheet microscopy stereotaxic atlas of the mouse brain
Article Snippet: .. Samples were incubated in Clearing Buffer A (LifeCanvas Technologies) overnight then actively delipidated using a LifeCanvas Technologies SmartClear II Pro device for 6 days using stochastic electrotransport ( ). ..

Article Title: The Duke Mouse Brain Atlas: MRI and light sheet microscopy stereotaxic atlas of the mouse brain.
Article Snippet: .. Samples were incubated in Clearing Buffer A (LifeCanvas Technologies) overnight then actively delipidated using a LifeCanvas Technologies SmartClear II Pro device for 6 days using stochastic electrotransport (52). ..

other:

Article Title: Neural circuit pathology driven by Shank3 mutation disrupts social behaviors
Article Snippet: SmartClear Clearing Buffer Type A & B , LifeCanvas Technologies , CAT #: SC-B2001.



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