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Promega reverse transcriptase buffer (5×) m515a1
Reverse Transcriptase Buffer (5×) M515a1, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+5/reverse+transcriptase+buffer++5/pmc04302722-321-45-49
Average 90 stars, based on 1 article reviews
reverse transcriptase buffer (5×) m515a1 - by Bioz Stars, 2026-09
90/100 stars

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Article Title: In vitro transcription and immobilized template analysis of preinitiation complexes
Article Snippet: Reagents list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 32 P-gamma-ATP (Perkin Elmer BLU002Z500UC) Ammonium Acetate 1 M pH 7 (Ammonium Acetate is a neutral salt) dNTPs (10 mM each) Geiger counter GE BiosciencesTM G50 de-salting column Hybridization Buffer (2×) Isopropyl Alcohol 10× PNK buffer list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 (Supplied with T4 PNK) Reverse Transcriptase list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 (Promega M5108) Reverse Transcriptase Buffer (5×) list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 (Promega M515A1) Reverse transcription primer list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 In this protocol, the primer begins +92 nucleotides downstream of the mRNA transcription start site.

Article Title: In vitro transcription and immobilized template analysis of preinitiation complexes
Article Snippet: Reagents 32 P-gamma-ATP (Perkin Elmer BLU002Z500UC) Ammonium Acetate 1 M pH 7 (Ammonium Acetate is a neutral salt) dNTPs (10 mM each) Geiger counter GE BiosciencesTM G50 de-salting column Hybridization Buffer (2×) Isopropyl Alcohol 10× PNK buffer (Supplied with T4 PNK) Reverse Transcriptase (Promega M5108) Reverse Transcriptase Buffer (5×) (Promega M515A1) Reverse transcription primer In this protocol, the primer begins +92 nucleotides downstream of the mRNA transcription start site.

Article Title: In vitro transcription and immobilized template analysis of preinitiation complexes
Article Snippet: 32 P-gamma-ATP (Perkin Elmer BLU002Z500UC) Ammonium Acetate 1 M pH 7 (Ammonium Acetate is a neutral salt) dNTPs (10 mM each) Geiger counter GE BiosciencesTM G50 de-salting column Hybridization Buffer (2×) Isopropyl Alcohol 10× PNK buffer (Supplied with T4 PNK) Reverse Transcriptase (Promega M5108) Reverse Transcriptase Buffer (5×) (Promega M515A1) Reverse transcription primer In this protocol, the primer begins +92 nucleotides downstream of the mRNA transcription start site.



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