microarray cassette (PEPperPRINT gmbh)
90
Structured Review
PEPperPRINT gmbh
microarray cassette
Microarray Cassette, supplied by PEPperPRINT gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blockit%E2%84%A2+microarray+blocking+buffer/microarray+cassette/pmc10974121-56-8-7
Average 90 stars, based on 1 article reviews
Microarray Cassette, supplied by PEPperPRINT gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blockit%E2%84%A2+microarray+blocking+buffer/microarray+cassette/pmc10974121-56-8-7
Average 90 stars, based on 1 article reviews
microarray cassette - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "Antigen-Heterologous Vaccination Regimen Triggers Alternate Antibody Targeting in SARS-CoV-2-DNA-Vaccinated Mice"
Article Title: Antigen-Heterologous Vaccination Regimen Triggers Alternate Antibody Targeting in SARS-CoV-2-DNA-Vaccinated Mice
Journal: Vaccines
doi: 10.3390/vaccines12030218
Figure Legend Snippet: Antibody binding to the receptor-binding domain (RBD). Peptide id. on the x -axis refers to the position of the last amino acid of each 10′mer peptide on the microarray. The y -axis indicates the level of antibody binding to each peptide measured as the average of duplicate determinations of spot fluorescence minus background fluorescence normalized according to a positive control included on each subarray. RBM = receptor-binding motif.
Techniques Used: Binding Assay, Microarray, Fluorescence, Positive Control
Figure Legend Snippet: Serum-binding antibody profiles. Antibody binding to 10′mer overlapping peptides covering the entire SARS-CoV-2 Index strain spike ectodomain. The x -axis refers to the position of the last amino acid of each 10′mer peptide on the microarray. The y -axis ( A , B ) indicates the level of antibody binding to each peptide measured as the average of duplicate determinations of spot fluorescence minus background fluorescence normalized according to a positive control included on each subarray. ( A ) Individual binding profiles from antibody binding in pools of serum samples stratified according to vaccination regimen. Major peaks are labeled. ( B ) Full overview of antibody binding in all three groups relative to the different SARS-CoV-2 spike protein domains. S1 subunit (residues 14–685); S2 subunit (residues 686–1273); NTD = N-terminal domain; RBD = receptor-binding domain; SD1, SD2, SD3 = sub-domain 1, 2, 3; S1/S2 = furin cleavage site; UH = upstream helix; S2’ cleavage site; FP = fusion peptide; CR = connecting region; HR1 and HR2 = heptad repeat sequence 1 and 2; CH = central helix; CD = connector domain; TM = transmembrane helix; CD = connector domain. ( C ) Heatmap showing serum antibody binding relative to the different SARS-CoV-2 spike protein domains in the Index-strain-vaccinated group, the Beta-VOC-vaccinated group, and the mixed-vaccinated group.
Techniques Used: Binding Assay, Microarray, Fluorescence, Positive Control, Labeling, Sequencing
Related Articles
Microarray:Article Title: Antigen-Heterologous Vaccination Regimen Triggers Alternate Antibody Targeting in SARS-CoV-2-DNA-Vaccinated Mice Article Snippet: After incubation at room temperature for 15 min, the washing buffer was removed using a pipette and each subarray was blocked in 400 μL of Rockland Blocking Buffer (MB-070, Rockland Immunochemicals, Pottstown, PA, USA) at room temperature for 30 min. To evaluate non-specific reactions, we then pre-treated the peptide array with a secondary antibody/streptavidin solution diluted in staining buffer (DPBS with 0.005% Tween20 and 10% blocking buffer, pH 7.4) as described below and scanned. .. The microarray was then reinserted into the Incubation:Article Title: Antigen-Heterologous Vaccination Regimen Triggers Alternate Antibody Targeting in SARS-CoV-2-DNA-Vaccinated Mice Article Snippet: After incubation at room temperature for 15 min, the washing buffer was removed using a pipette and each subarray was blocked in 400 μL of Rockland Blocking Buffer (MB-070, Rockland Immunochemicals, Pottstown, PA, USA) at room temperature for 30 min. To evaluate non-specific reactions, we then pre-treated the peptide array with a secondary antibody/streptavidin solution diluted in staining buffer (DPBS with 0.005% Tween20 and 10% blocking buffer, pH 7.4) as described below and scanned. .. The microarray was then reinserted into the Staining:Article Title: Antigen-Heterologous Vaccination Regimen Triggers Alternate Antibody Targeting in SARS-CoV-2-DNA-Vaccinated Mice Article Snippet: After incubation at room temperature for 15 min, the washing buffer was removed using a pipette and each subarray was blocked in 400 μL of Rockland Blocking Buffer (MB-070, Rockland Immunochemicals, Pottstown, PA, USA) at room temperature for 30 min. To evaluate non-specific reactions, we then pre-treated the peptide array with a secondary antibody/streptavidin solution diluted in staining buffer (DPBS with 0.005% Tween20 and 10% blocking buffer, pH 7.4) as described below and scanned. .. The microarray was then reinserted into the |
