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blocking solution containing dapi s2110  (Beijing Solarbio Science)


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    Structured Review

    Beijing Solarbio Science blocking solution containing dapi s2110
    Blocking Solution Containing Dapi S2110, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+solution+containing+dapi/dapi+s2110/pm40157277-83-33-34
    Average 90 stars, based on 1 article reviews
    blocking solution containing dapi s2110 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Hesperidin alleviates pulmonary fibrosis by regulating EI24-mediated autophagy.
    Article Snippet: Next, cells were incubated with the rabbit polyclonal to lc3B (1:200, #ab48394, abcam) at 4 °c overnight. subsequently, cells were washed with PBs for three times and treated with Goat anti-Rabbit igG h&l (alexa Fluor® 488) (1:1000, #ab150077, abcam) for 1 h at room temperature. .. Finally, cells were stained with Mounting Medium, antifading with DaPi (#s2110, solarbio) and photographed under a fluorescence microscopy (#iX71, Olympus, tokyo, Japan). ..

    Article Title: USP10 protects against pressure overload-induced mitochondrial morphofunctional defects and pathological cardiac hypertrophy through stabilizing cytoplasmic Mfn2
    Article Snippet: .. The cells were incubated overnight with USP10 and Mfn2 antibodies, followed by incubation with fluorescent secondary antibodies Alexa Fluor 488 (Beyotime, P0188) and Alexa Fluor 555 (Beyotime, A0453) at 37 °C for 1 h. Cell nuclei were stained with DAPI (Solarbio, S2110), and F-actin was stained with Actin-Tracker Red-Rhodamine (Beyotime, C2207S). .. Microscopic images were magnified × 400 using an intelligent biological microscope (OLYMPUS BX53).

    Article Title: STAT1-IFITM3 promotes autophagy in epithelial cells to control Cryptosporidium parvum infection
    Article Snippet: For cytoskeleton staining, 200 μl of iFluor 546-Ghost cyclic peptide F-actin (Creative Biolabs) working solution was added to the cells and stained at RT for 15 min, followed by PBS washing. .. Subsequently, a drop of antifading mounting medium with the nucleic acid stain DAPI (Solarbio) was added to seal the slide. .. Finally, cell immunofluorescence was assessed using the Invitrogen EVOS FL Auto Cell Imaging System or a Nikon eclipse Ti Confocal Microscope.

    Fluorescence:

    Article Title: Hesperidin alleviates pulmonary fibrosis by regulating EI24-mediated autophagy.
    Article Snippet: Next, cells were incubated with the rabbit polyclonal to lc3B (1:200, #ab48394, abcam) at 4 °c overnight. subsequently, cells were washed with PBs for three times and treated with Goat anti-Rabbit igG h&l (alexa Fluor® 488) (1:1000, #ab150077, abcam) for 1 h at room temperature. .. Finally, cells were stained with Mounting Medium, antifading with DaPi (#s2110, solarbio) and photographed under a fluorescence microscopy (#iX71, Olympus, tokyo, Japan). ..

    Article Title: CTSK as a Collagen Degradation Marker Induces Gingival Recession During High-Force Orthodontic Tooth Movement
    Article Snippet: .. The sections were mounted with DAPI-containing antifade mounting medium (Solarbio, Beijing), pressed overnight, and observed and imaged under an upright fluorescence microscope (Leica, DM4B). ..

    Microscopy:

    Article Title: Hesperidin alleviates pulmonary fibrosis by regulating EI24-mediated autophagy.
    Article Snippet: Next, cells were incubated with the rabbit polyclonal to lc3B (1:200, #ab48394, abcam) at 4 °c overnight. subsequently, cells were washed with PBs for three times and treated with Goat anti-Rabbit igG h&l (alexa Fluor® 488) (1:1000, #ab150077, abcam) for 1 h at room temperature. .. Finally, cells were stained with Mounting Medium, antifading with DaPi (#s2110, solarbio) and photographed under a fluorescence microscopy (#iX71, Olympus, tokyo, Japan). ..

    Article Title: CTSK as a Collagen Degradation Marker Induces Gingival Recession During High-Force Orthodontic Tooth Movement
    Article Snippet: .. The sections were mounted with DAPI-containing antifade mounting medium (Solarbio, Beijing), pressed overnight, and observed and imaged under an upright fluorescence microscope (Leica, DM4B). ..

    other:

    Article Title: Protective effect and mechanism of Zhuang medicine Tinosporae Radix on LPS-induced neuroinflammation.
    Article Snippet: Ethnopharmacological relevance: In Zhuang medicine, Alzheimer’s disease (AD) is termed “Qiaowuwa’, attributed to the accumulation of heat-toxin in the Huolu (Fire Pathway) and malnutrition of Qiaowu.. The dried tuberous roots of Tinospora sagittata (Oliv.). Gagnep., known as “Tinosporae Radix’, are documented in Zhuang medicinal texts for their bitter flavor and cold nature, which clear heat-toxin, regulate Huolu, and treat inflammatory disorders.

    Article Title: Vascularized Bioengineered Kidney Using Decellularized Scaffold Recellularized with human Placenta-Derived Angiogenic stem Cells and Kidney Organoids
    Article Snippet: Wash with PBST, add DAPI (Solarbio, S2110), mount the slides, and examine using a laser confocal microscope (Zeiss, LSM 900).

    Article Title: The substructure of the endothelial glycocalyx in rat aorta and its interaction with the low-density lipoproteins (LDL).
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Tanshinone IIA induces oxidative stress in trophoblast cells and enhances copper dependent death.
    Article Snippet: After washing, antifading mounting medium containing DAPI (Solarbio, S2110) was added dropwise to the slides.

    Incubation:

    Article Title: USP10 protects against pressure overload-induced mitochondrial morphofunctional defects and pathological cardiac hypertrophy through stabilizing cytoplasmic Mfn2
    Article Snippet: .. The cells were incubated overnight with USP10 and Mfn2 antibodies, followed by incubation with fluorescent secondary antibodies Alexa Fluor 488 (Beyotime, P0188) and Alexa Fluor 555 (Beyotime, A0453) at 37 °C for 1 h. Cell nuclei were stained with DAPI (Solarbio, S2110), and F-actin was stained with Actin-Tracker Red-Rhodamine (Beyotime, C2207S). .. Microscopic images were magnified × 400 using an intelligent biological microscope (OLYMPUS BX53).



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    (A) The primary screen was performed on MCF10A cells in a 384-well format. Genes belonging to a subset of the human genome were knocked down in six replicate plates for 72 h by RNAi along with the listed controls. In a duplicate experiment, cells were stimulated with EGTA. Control wells were distributed on the plates as shown. NT: non-targeting KD – cells were transfected with non-targeting siRNA. No siRNA: cells received RNAiMAX only. Positive control (pos ctrl) – cells were transfected with siRNA against PSENEN. Transfection pos ctrl: cells were transfected with siRNA against polo-like kinase 1 (PLK1) as a cytotoxic indicator of transfection efficiency. Outer wells (yellow) were filled with media to prevent evaporation in experimental wells. (B) To assess endogenous NOTCH1 localization, cells in plates were subjected to automated IF <t>and</t> <t>DAPI/phalloidin</t> labeling to demarcate nuclei and the cell cortex, respectively. The script steps of the image acquisition and analysis developed in-house are as follows: (1) The nuclei were segmented based on DAPI signal. (2) The cell surface was segmented using the phalloidin signal and overlaid on the NOTCH1 channel (3) to quantify levels of cell surface NOTCH1 (4), and the area between the cell cortex and the nuclei was used to determine levels of cytoplasmic NOTCH1 (5). (6) The phalloidin mask was also used to count cells and establish cell-to-cell boundaries. (7) Nuclear size was used as readout of cell viability as compact; pyknotic nuclei identify dead cells. Using such a pipeline, each gene KD was defined by its effects on the amount of NOTCH1 in the cell cortex, in the cytoplasm or in the nucleus.
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    Two-dimensional immunofluorescence stain of isolated bovine muscle satellite cells (BSCs). ( A ) Proliferating bovine satellite stained for <t>DAPI,</t> actin cytoskeleton (Phalloidin), and Pax7, a nuclear marker of satellite cells. Stains show a highly pure satellite cell population, following isolation and pre-plating protocol. ( B ) Following one week of differentiation, cells were stained for DAPI, actin cytoskeleton (Phalloidin), <t>and</t> <t>Troponin</t> T (CT3), a marker of myogenesis. Scale bars are 200 µm.
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    Image Search Results


    (A) The primary screen was performed on MCF10A cells in a 384-well format. Genes belonging to a subset of the human genome were knocked down in six replicate plates for 72 h by RNAi along with the listed controls. In a duplicate experiment, cells were stimulated with EGTA. Control wells were distributed on the plates as shown. NT: non-targeting KD – cells were transfected with non-targeting siRNA. No siRNA: cells received RNAiMAX only. Positive control (pos ctrl) – cells were transfected with siRNA against PSENEN. Transfection pos ctrl: cells were transfected with siRNA against polo-like kinase 1 (PLK1) as a cytotoxic indicator of transfection efficiency. Outer wells (yellow) were filled with media to prevent evaporation in experimental wells. (B) To assess endogenous NOTCH1 localization, cells in plates were subjected to automated IF and DAPI/phalloidin labeling to demarcate nuclei and the cell cortex, respectively. The script steps of the image acquisition and analysis developed in-house are as follows: (1) The nuclei were segmented based on DAPI signal. (2) The cell surface was segmented using the phalloidin signal and overlaid on the NOTCH1 channel (3) to quantify levels of cell surface NOTCH1 (4), and the area between the cell cortex and the nuclei was used to determine levels of cytoplasmic NOTCH1 (5). (6) The phalloidin mask was also used to count cells and establish cell-to-cell boundaries. (7) Nuclear size was used as readout of cell viability as compact; pyknotic nuclei identify dead cells. Using such a pipeline, each gene KD was defined by its effects on the amount of NOTCH1 in the cell cortex, in the cytoplasm or in the nucleus.

    Journal: Life Science Alliance

    Article Title: Novel determinants of NOTCH1 trafficking and signaling in breast epithelial cells

    doi: 10.26508/lsa.202403122

    Figure Lengend Snippet: (A) The primary screen was performed on MCF10A cells in a 384-well format. Genes belonging to a subset of the human genome were knocked down in six replicate plates for 72 h by RNAi along with the listed controls. In a duplicate experiment, cells were stimulated with EGTA. Control wells were distributed on the plates as shown. NT: non-targeting KD – cells were transfected with non-targeting siRNA. No siRNA: cells received RNAiMAX only. Positive control (pos ctrl) – cells were transfected with siRNA against PSENEN. Transfection pos ctrl: cells were transfected with siRNA against polo-like kinase 1 (PLK1) as a cytotoxic indicator of transfection efficiency. Outer wells (yellow) were filled with media to prevent evaporation in experimental wells. (B) To assess endogenous NOTCH1 localization, cells in plates were subjected to automated IF and DAPI/phalloidin labeling to demarcate nuclei and the cell cortex, respectively. The script steps of the image acquisition and analysis developed in-house are as follows: (1) The nuclei were segmented based on DAPI signal. (2) The cell surface was segmented using the phalloidin signal and overlaid on the NOTCH1 channel (3) to quantify levels of cell surface NOTCH1 (4), and the area between the cell cortex and the nuclei was used to determine levels of cytoplasmic NOTCH1 (5). (6) The phalloidin mask was also used to count cells and establish cell-to-cell boundaries. (7) Nuclear size was used as readout of cell viability as compact; pyknotic nuclei identify dead cells. Using such a pipeline, each gene KD was defined by its effects on the amount of NOTCH1 in the cell cortex, in the cytoplasm or in the nucleus.

    Article Snippet: Next, the cells were incubated for 1 h with 1% BSA blocking solution containing DAPI at 1:4,500 (Cat #: D9542; Merck Life Sciences), phalloidin at 1:350 (Cat #: P5282; Merck Life Sciences), and Alexa Fluor 488 anti-rat secondary antibody at 1:400 (Cat #: A21208; Thermo Fisher Scientific).

    Techniques: Control, Transfection, Positive Control, Evaporation, Labeling

    Two-dimensional immunofluorescence stain of isolated bovine muscle satellite cells (BSCs). ( A ) Proliferating bovine satellite stained for DAPI, actin cytoskeleton (Phalloidin), and Pax7, a nuclear marker of satellite cells. Stains show a highly pure satellite cell population, following isolation and pre-plating protocol. ( B ) Following one week of differentiation, cells were stained for DAPI, actin cytoskeleton (Phalloidin), and Troponin T (CT3), a marker of myogenesis. Scale bars are 200 µm.

    Journal: Foods

    Article Title: Extracellular Heme Proteins Influence Bovine Myosatellite Cell Proliferation and the Color of Cell-Based Meat

    doi: 10.3390/foods8100521

    Figure Lengend Snippet: Two-dimensional immunofluorescence stain of isolated bovine muscle satellite cells (BSCs). ( A ) Proliferating bovine satellite stained for DAPI, actin cytoskeleton (Phalloidin), and Pax7, a nuclear marker of satellite cells. Stains show a highly pure satellite cell population, following isolation and pre-plating protocol. ( B ) Following one week of differentiation, cells were stained for DAPI, actin cytoskeleton (Phalloidin), and Troponin T (CT3), a marker of myogenesis. Scale bars are 200 µm.

    Article Snippet: For BAMs, secondary antibodies for Troponin T were diluted in blocking solution containing DAPI (Thermo Fisher, #62248, 1:1000) and added to constructs for 1 h at room temperature.

    Techniques: Immunofluorescence, Staining, Isolation, Marker

    Confocal immunofluorescent imaging of BAMs. BAMs generated from BSC, BSC + Hb, or BSC + Mb (3 mg/mL for both heme proteins) were stained after eight days of differentiation for DAPI, actin cytoskeleton (Phalloidin), and Troponin T (CT3), a marker of myogenesis. Images show multinucleated myotube formation in BSC and BSC + Mb constructs, though not in BSC + Hb constructs. Scale bars are 200 µm.

    Journal: Foods

    Article Title: Extracellular Heme Proteins Influence Bovine Myosatellite Cell Proliferation and the Color of Cell-Based Meat

    doi: 10.3390/foods8100521

    Figure Lengend Snippet: Confocal immunofluorescent imaging of BAMs. BAMs generated from BSC, BSC + Hb, or BSC + Mb (3 mg/mL for both heme proteins) were stained after eight days of differentiation for DAPI, actin cytoskeleton (Phalloidin), and Troponin T (CT3), a marker of myogenesis. Images show multinucleated myotube formation in BSC and BSC + Mb constructs, though not in BSC + Hb constructs. Scale bars are 200 µm.

    Article Snippet: For BAMs, secondary antibodies for Troponin T were diluted in blocking solution containing DAPI (Thermo Fisher, #62248, 1:1000) and added to constructs for 1 h at room temperature.

    Techniques: Imaging, Generated, Staining, Marker, Construct