Review



antifluorescence quenching blocking solution  (Beyotime)


Bioz Verified Symbol Beyotime is a verified supplier
Bioz Manufacturer Symbol Beyotime manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Beyotime antifluorescence quenching blocking solution
    Antifluorescence Quenching Blocking Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2090 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+solution+containing+dapi/DAPI+Staining+Solution/pmc10053273-68-17-21
    Average 99 stars, based on 2090 article reviews
    antifluorescence quenching blocking solution - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Labeling:

    Article Title: TRPM7 function in ischemia-reperfusion renal injury and its regulatory mechanism of PARthanatos
    Article Snippet: .. Upon completion of all labeling procedures, DAPI staining solution (C1005, Beyotime, Shanghai, China) was added for 5 min of nuclear staining in the dark, and residual DAPI was washed off with PBS. .. Slides were treated with tissue autofluorescence quencher for 5 min in darkness and rinsed under running tap water for 10 min, then mounted with anti-fluorescence quenching mounting medium (P0126, Beyotime, Shanghai, China).

    Staining:

    Article Title: TRPM7 function in ischemia-reperfusion renal injury and its regulatory mechanism of PARthanatos
    Article Snippet: .. Upon completion of all labeling procedures, DAPI staining solution (C1005, Beyotime, Shanghai, China) was added for 5 min of nuclear staining in the dark, and residual DAPI was washed off with PBS. .. Slides were treated with tissue autofluorescence quencher for 5 min in darkness and rinsed under running tap water for 10 min, then mounted with anti-fluorescence quenching mounting medium (P0126, Beyotime, Shanghai, China).

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: After ten washes, embryos were placed into diluted secondary antibodies (anti-mouse IgG 488, Abbkine, A23210, 1:500; anti-rabbit IgG 549, Abbkine, A23320, 1:500) for 1 h at room temperature, followed by another ten washes. .. Finally, embryos were incubated in DAPI staining solution for 3 min and washed ten times before mounting in a 2-μL drop of mounting medium (Beyotime, P0126) on a glass slide. ..

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: .. The nuclei of TE biopsy cells were then dyed by 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Beyotime, P0131), and captured using an inverted microscope equipped with fluorescence illumination system (Nikon, Eclipse Ti2-U). ..

    Article Title: Tectorigenin reprograms macrophages through targeting OXCT1-mediated ketone metabolism to suppress IFN-I signaling and ameliorate myocardial ischemia-reperfusion injury
    Article Snippet: .. The sections were rinsed with PBS for another 3 cycles, 5 min per wash. 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Cat. No.: C1005; Beyotime Jo urn al Pr e-p roo f 7 / 40 Biotechnology) was added dropwise and incubated at room temperature in the dark for 5 min for nuclear counterstaining. ..

    Article Title: Dexmedetomidine Activates AMPK/SIRT1 Pathway to Reduce Inflammatory Levels and Neuronal Pyroptosis to Alleviate Neonatal Hypoxic-Ischemic Brain Damage.
    Article Snippet: Neonatal hypoxic-ischemic brain damage (HIBD) is the leading cause of neonatal death and long-term neurological dysfunction.. Dexmedetomidine (DEX) has been shown to have neuroprotective effects, but its mechanisms in HIBD remain incompletely understood.. This study aimed to explore whether DEX inhibits neuroinflammation and pyroptosis via activating adenosine monophosphate-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) axis, thereby reducing HIBD.

    Article Title: Hierarchically sequential targeting nanoplatform suppresses NETosis-promoted angiogenesis for corneal neovascularization therapy.
    Article Snippet: The Phorbol 12-myristate 13-acetate (PMA, S1819) was purchased from Beyotime (Shanghai, China). .. The 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride Jo ur na l P re -p ro of (DAPI, C1005) Staining Solution was purchased from Beyotime (Shanghai, China). .. The clamidine hydrochloride was purchased from Sparkjade (SJ-MX0367A, Shandong, China).

    Article Title: Butyrate counteracts maternal obesity-induced cardiac defects by promoting PLEKHG3-actin binding
    Article Snippet: Vector TrueVIEW kit , Vector laboratories , SP-8400. .. DAPI Staining Solution , Beyotime , C1006. .. mTeSR1 medium , STEMCELL , 85850.

    Incubation:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: After ten washes, embryos were placed into diluted secondary antibodies (anti-mouse IgG 488, Abbkine, A23210, 1:500; anti-rabbit IgG 549, Abbkine, A23320, 1:500) for 1 h at room temperature, followed by another ten washes. .. Finally, embryos were incubated in DAPI staining solution for 3 min and washed ten times before mounting in a 2-μL drop of mounting medium (Beyotime, P0126) on a glass slide. ..

    Article Title: Tectorigenin reprograms macrophages through targeting OXCT1-mediated ketone metabolism to suppress IFN-I signaling and ameliorate myocardial ischemia-reperfusion injury
    Article Snippet: .. The sections were rinsed with PBS for another 3 cycles, 5 min per wash. 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Cat. No.: C1005; Beyotime Jo urn al Pr e-p roo f 7 / 40 Biotechnology) was added dropwise and incubated at room temperature in the dark for 5 min for nuclear counterstaining. ..

    Article Title: Dexmedetomidine Activates AMPK/SIRT1 Pathway to Reduce Inflammatory Levels and Neuronal Pyroptosis to Alleviate Neonatal Hypoxic-Ischemic Brain Damage.
    Article Snippet: Neonatal hypoxic-ischemic brain damage (HIBD) is the leading cause of neonatal death and long-term neurological dysfunction.. Dexmedetomidine (DEX) has been shown to have neuroprotective effects, but its mechanisms in HIBD remain incompletely understood.. This study aimed to explore whether DEX inhibits neuroinflammation and pyroptosis via activating adenosine monophosphate-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) axis, thereby reducing HIBD.

    Glass:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: After ten washes, embryos were placed into diluted secondary antibodies (anti-mouse IgG 488, Abbkine, A23210, 1:500; anti-rabbit IgG 549, Abbkine, A23320, 1:500) for 1 h at room temperature, followed by another ten washes. .. Finally, embryos were incubated in DAPI staining solution for 3 min and washed ten times before mounting in a 2-μL drop of mounting medium (Beyotime, P0126) on a glass slide. ..

    Inverted Microscopy:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: .. The nuclei of TE biopsy cells were then dyed by 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Beyotime, P0131), and captured using an inverted microscope equipped with fluorescence illumination system (Nikon, Eclipse Ti2-U). ..

    Fluorescence:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: .. The nuclei of TE biopsy cells were then dyed by 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Beyotime, P0131), and captured using an inverted microscope equipped with fluorescence illumination system (Nikon, Eclipse Ti2-U). ..

    other:

    Article Title: Lacticaseibacillus rhamnosus GG enhances gefitinib efficacy and reduces treatment-related toxicity via propionyl-L-carnitine regulation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Propionyl-L-carnitine (PLC) Aladdin Biochemical Technology, Shanghai, China CAS: 20064-19-1 Acetonitrile Xilong Scientific Co., Ltd., China CAS: 75-05-8 Formic acid Xilong Scientific Co., Ltd., China CAS: 64-18-6 Methotrexate Yuanye Bio-Technology, Shanghai, China CAS: 59-05-2 Luciferin sodium salt Yuanye Bio-Technology, Shanghai, China Cat#: B25437 Sodium carboxymethylcellulose (CMC-Na) Solarbio Life Sciences, Beijing, China CAS: 9004-32-4 Universal Tissue Fixative (Neutral) Servicebio, Wuhan, China Cat#: G1101 Trypsin Gibco Cat#: 25200-056 Fetal bovine serum Gibco Cat#: A5669701 Penicillin-streptomycin Gibco Cat#: 15140122 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat#: 11965092 RPMI 1640 medium Gibco Cat#: C11875500BT BSA blocking solution Thermo Fisher Scientific Cat#: 37525 RIPA lysis buffer Thermo Fisher Scientific Cat#: 89901 RIPA buffer Beyotime Biotechnology Cat#: P0013B PMSF Beyotime Biotechnology Cat#: ST507 Protease/phosphatase inhibitor cocktail Beyotime Biotechnology Cat#: P1045 0.1% Triton X-100 Beyotime Biotechnology Cat#: P0096 DAPI staining solution Beyotime Biotechnology Cat#: C1006 Anhydrous Sodium Sulfate Xilong Scientific Co., Ltd., China CAS: 7757-82-6 Sodium Chloride (NaCl) Xilong Scientific Co., Ltd., China CAS: 7647-14-5 Dilute Sulfuric Acid (H2SO4) Xilong Scientific Co., Ltd., China CAS: 7664-93-9 n-Hexane Xilong Scientific Co., Ltd., China CAS: 110-54-3 Collagenase Type IV Sigma-Aldrich Cat#: C4-28 DNase I Beyotime Biotechnology Cat#: D7073 Critical commercial assays CCK-8 Kit MedChemExpress Cat#: HY-K0301 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat#: C0009S Annexin V-FITC/PI Apoptosis Detection Kit Huiyucheng Biotechnology, Wuhan, China Cat#: HYC2019 BCA Protein Assay Kit Thermo Fisher Scientific Cat#: 23225 TUNEL Assay Kit Beyotime Biotechnology Cat#: C1098 DAB Substrate Kit Beyotime Biotechnology Cat# P0202 Pierce ECL Western Blotting Substrate Thermo Fisher Scientific Cat#: 32106 CEA ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY9M0916 CYFRA21-1 ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY926701a GLUT1 ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY9NM11861 LDHA ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY9NM1898 Mitochondrial Complex I Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737601a Mitochondrial Complex II Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737701a Mitochondrial Complex III Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737801a Mitochondrial Complex IV Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737901a Mitochondrial Complex V Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737011a Propionyl-L-carnitine (PLC) ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY972791a Mouse ROS ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XYM9437001 Mouse IKKα ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY9438821a (Continued on next page) 18 Cell Reports 45, 118068, October 27, 2026



    Similar Products

    blocking solution containing dapi s2110  (Beijing Solarbio Science)
    90
    Beijing Solarbio Science blocking solution containing dapi s2110
    Blocking Solution Containing Dapi S2110, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+solution+containing+dapi/dapi+s2110/pm40157277-83-33-34
    Average 90 stars, based on 1 article reviews
    blocking solution containing dapi s2110 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    bsa blocking solution containing dapi  (Merck & Co)
    90
    Merck & Co bsa blocking solution containing dapi
    (A) The primary screen was performed on MCF10A cells in a 384-well format. Genes belonging to a subset of the human genome were knocked down in six replicate plates for 72 h by RNAi along with the listed controls. In a duplicate experiment, cells were stimulated with EGTA. Control wells were distributed on the plates as shown. NT: non-targeting KD – cells were transfected with non-targeting siRNA. No siRNA: cells received RNAiMAX only. Positive control (pos ctrl) – cells were transfected with siRNA against PSENEN. Transfection pos ctrl: cells were transfected with siRNA against polo-like kinase 1 (PLK1) as a cytotoxic indicator of transfection efficiency. Outer wells (yellow) were filled with media to prevent evaporation in experimental wells. (B) To assess endogenous NOTCH1 localization, cells in plates were subjected to automated IF <t>and</t> <t>DAPI/phalloidin</t> labeling to demarcate nuclei and the cell cortex, respectively. The script steps of the image acquisition and analysis developed in-house are as follows: (1) The nuclei were segmented based on DAPI signal. (2) The cell surface was segmented using the phalloidin signal and overlaid on the NOTCH1 channel (3) to quantify levels of cell surface NOTCH1 (4), and the area between the cell cortex and the nuclei was used to determine levels of cytoplasmic NOTCH1 (5). (6) The phalloidin mask was also used to count cells and establish cell-to-cell boundaries. (7) Nuclear size was used as readout of cell viability as compact; pyknotic nuclei identify dead cells. Using such a pipeline, each gene KD was defined by its effects on the amount of NOTCH1 in the cell cortex, in the cytoplasm or in the nucleus.
    Bsa Blocking Solution Containing Dapi, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+solution+containing+dapi/bsa+dapi/pmc11633778-295-14-20
    Average 90 stars, based on 1 article reviews
    bsa blocking solution containing dapi - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    dapi-containing blocking solution p36966  (Thermo Fisher)
    90
    Thermo Fisher dapi-containing blocking solution p36966
    (A) The primary screen was performed on MCF10A cells in a 384-well format. Genes belonging to a subset of the human genome were knocked down in six replicate plates for 72 h by RNAi along with the listed controls. In a duplicate experiment, cells were stimulated with EGTA. Control wells were distributed on the plates as shown. NT: non-targeting KD – cells were transfected with non-targeting siRNA. No siRNA: cells received RNAiMAX only. Positive control (pos ctrl) – cells were transfected with siRNA against PSENEN. Transfection pos ctrl: cells were transfected with siRNA against polo-like kinase 1 (PLK1) as a cytotoxic indicator of transfection efficiency. Outer wells (yellow) were filled with media to prevent evaporation in experimental wells. (B) To assess endogenous NOTCH1 localization, cells in plates were subjected to automated IF <t>and</t> <t>DAPI/phalloidin</t> labeling to demarcate nuclei and the cell cortex, respectively. The script steps of the image acquisition and analysis developed in-house are as follows: (1) The nuclei were segmented based on DAPI signal. (2) The cell surface was segmented using the phalloidin signal and overlaid on the NOTCH1 channel (3) to quantify levels of cell surface NOTCH1 (4), and the area between the cell cortex and the nuclei was used to determine levels of cytoplasmic NOTCH1 (5). (6) The phalloidin mask was also used to count cells and establish cell-to-cell boundaries. (7) Nuclear size was used as readout of cell viability as compact; pyknotic nuclei identify dead cells. Using such a pipeline, each gene KD was defined by its effects on the amount of NOTCH1 in the cell cortex, in the cytoplasm or in the nucleus.
    Dapi Containing Blocking Solution P36966, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+solution+containing+dapi/prolong+diamond+antifade+mountant/pmc10870295-61-7-10
    Average 90 stars, based on 1 article reviews
    dapi-containing blocking solution p36966 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    anti-fluorescence quenching blocking solution containing dapi  (SouthernBiotech)
    90
    SouthernBiotech anti-fluorescence quenching blocking solution containing dapi
    (A) The primary screen was performed on MCF10A cells in a 384-well format. Genes belonging to a subset of the human genome were knocked down in six replicate plates for 72 h by RNAi along with the listed controls. In a duplicate experiment, cells were stimulated with EGTA. Control wells were distributed on the plates as shown. NT: non-targeting KD – cells were transfected with non-targeting siRNA. No siRNA: cells received RNAiMAX only. Positive control (pos ctrl) – cells were transfected with siRNA against PSENEN. Transfection pos ctrl: cells were transfected with siRNA against polo-like kinase 1 (PLK1) as a cytotoxic indicator of transfection efficiency. Outer wells (yellow) were filled with media to prevent evaporation in experimental wells. (B) To assess endogenous NOTCH1 localization, cells in plates were subjected to automated IF <t>and</t> <t>DAPI/phalloidin</t> labeling to demarcate nuclei and the cell cortex, respectively. The script steps of the image acquisition and analysis developed in-house are as follows: (1) The nuclei were segmented based on DAPI signal. (2) The cell surface was segmented using the phalloidin signal and overlaid on the NOTCH1 channel (3) to quantify levels of cell surface NOTCH1 (4), and the area between the cell cortex and the nuclei was used to determine levels of cytoplasmic NOTCH1 (5). (6) The phalloidin mask was also used to count cells and establish cell-to-cell boundaries. (7) Nuclear size was used as readout of cell viability as compact; pyknotic nuclei identify dead cells. Using such a pipeline, each gene KD was defined by its effects on the amount of NOTCH1 in the cell cortex, in the cytoplasm or in the nucleus.
    Anti Fluorescence Quenching Blocking Solution Containing Dapi, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+solution+containing+dapi/dapi+solution+containing+an+anti+fluorescence+quencher/pm37394123-100-10-16
    Average 90 stars, based on 1 article reviews
    anti-fluorescence quenching blocking solution containing dapi - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    antifluorescence quenching blocking solution containing dapi  (Beyotime)
    90
    Beyotime antifluorescence quenching blocking solution containing dapi
    (A) The primary screen was performed on MCF10A cells in a 384-well format. Genes belonging to a subset of the human genome were knocked down in six replicate plates for 72 h by RNAi along with the listed controls. In a duplicate experiment, cells were stimulated with EGTA. Control wells were distributed on the plates as shown. NT: non-targeting KD – cells were transfected with non-targeting siRNA. No siRNA: cells received RNAiMAX only. Positive control (pos ctrl) – cells were transfected with siRNA against PSENEN. Transfection pos ctrl: cells were transfected with siRNA against polo-like kinase 1 (PLK1) as a cytotoxic indicator of transfection efficiency. Outer wells (yellow) were filled with media to prevent evaporation in experimental wells. (B) To assess endogenous NOTCH1 localization, cells in plates were subjected to automated IF <t>and</t> <t>DAPI/phalloidin</t> labeling to demarcate nuclei and the cell cortex, respectively. The script steps of the image acquisition and analysis developed in-house are as follows: (1) The nuclei were segmented based on DAPI signal. (2) The cell surface was segmented using the phalloidin signal and overlaid on the NOTCH1 channel (3) to quantify levels of cell surface NOTCH1 (4), and the area between the cell cortex and the nuclei was used to determine levels of cytoplasmic NOTCH1 (5). (6) The phalloidin mask was also used to count cells and establish cell-to-cell boundaries. (7) Nuclear size was used as readout of cell viability as compact; pyknotic nuclei identify dead cells. Using such a pipeline, each gene KD was defined by its effects on the amount of NOTCH1 in the cell cortex, in the cytoplasm or in the nucleus.
    Antifluorescence Quenching Blocking Solution Containing Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+solution+containing+dapi/anti+fluorescence+quencher+containing+dapi/pmc08567594-76-18-13
    Average 90 stars, based on 1 article reviews
    antifluorescence quenching blocking solution containing dapi - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    blocking solution containing dapi  (Thermo Fisher)
    90
    Thermo Fisher blocking solution containing dapi
    Two-dimensional immunofluorescence stain of isolated bovine muscle satellite cells (BSCs). ( A ) Proliferating bovine satellite stained for <t>DAPI,</t> actin cytoskeleton (Phalloidin), and Pax7, a nuclear marker of satellite cells. Stains show a highly pure satellite cell population, following isolation and pre-plating protocol. ( B ) Following one week of differentiation, cells were stained for DAPI, actin cytoskeleton (Phalloidin), <t>and</t> <t>Troponin</t> T (CT3), a marker of myogenesis. Scale bars are 200 µm.
    Blocking Solution Containing Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+solution+containing+dapi/DyLight+755+Maleimide/pmc06835221-116-13-14
    Average 90 stars, based on 1 article reviews
    blocking solution containing dapi - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) The primary screen was performed on MCF10A cells in a 384-well format. Genes belonging to a subset of the human genome were knocked down in six replicate plates for 72 h by RNAi along with the listed controls. In a duplicate experiment, cells were stimulated with EGTA. Control wells were distributed on the plates as shown. NT: non-targeting KD – cells were transfected with non-targeting siRNA. No siRNA: cells received RNAiMAX only. Positive control (pos ctrl) – cells were transfected with siRNA against PSENEN. Transfection pos ctrl: cells were transfected with siRNA against polo-like kinase 1 (PLK1) as a cytotoxic indicator of transfection efficiency. Outer wells (yellow) were filled with media to prevent evaporation in experimental wells. (B) To assess endogenous NOTCH1 localization, cells in plates were subjected to automated IF and DAPI/phalloidin labeling to demarcate nuclei and the cell cortex, respectively. The script steps of the image acquisition and analysis developed in-house are as follows: (1) The nuclei were segmented based on DAPI signal. (2) The cell surface was segmented using the phalloidin signal and overlaid on the NOTCH1 channel (3) to quantify levels of cell surface NOTCH1 (4), and the area between the cell cortex and the nuclei was used to determine levels of cytoplasmic NOTCH1 (5). (6) The phalloidin mask was also used to count cells and establish cell-to-cell boundaries. (7) Nuclear size was used as readout of cell viability as compact; pyknotic nuclei identify dead cells. Using such a pipeline, each gene KD was defined by its effects on the amount of NOTCH1 in the cell cortex, in the cytoplasm or in the nucleus.

    Journal: Life Science Alliance

    Article Title: Novel determinants of NOTCH1 trafficking and signaling in breast epithelial cells

    doi: 10.26508/lsa.202403122

    Figure Lengend Snippet: (A) The primary screen was performed on MCF10A cells in a 384-well format. Genes belonging to a subset of the human genome were knocked down in six replicate plates for 72 h by RNAi along with the listed controls. In a duplicate experiment, cells were stimulated with EGTA. Control wells were distributed on the plates as shown. NT: non-targeting KD – cells were transfected with non-targeting siRNA. No siRNA: cells received RNAiMAX only. Positive control (pos ctrl) – cells were transfected with siRNA against PSENEN. Transfection pos ctrl: cells were transfected with siRNA against polo-like kinase 1 (PLK1) as a cytotoxic indicator of transfection efficiency. Outer wells (yellow) were filled with media to prevent evaporation in experimental wells. (B) To assess endogenous NOTCH1 localization, cells in plates were subjected to automated IF and DAPI/phalloidin labeling to demarcate nuclei and the cell cortex, respectively. The script steps of the image acquisition and analysis developed in-house are as follows: (1) The nuclei were segmented based on DAPI signal. (2) The cell surface was segmented using the phalloidin signal and overlaid on the NOTCH1 channel (3) to quantify levels of cell surface NOTCH1 (4), and the area between the cell cortex and the nuclei was used to determine levels of cytoplasmic NOTCH1 (5). (6) The phalloidin mask was also used to count cells and establish cell-to-cell boundaries. (7) Nuclear size was used as readout of cell viability as compact; pyknotic nuclei identify dead cells. Using such a pipeline, each gene KD was defined by its effects on the amount of NOTCH1 in the cell cortex, in the cytoplasm or in the nucleus.

    Article Snippet: Next, the cells were incubated for 1 h with 1% BSA blocking solution containing DAPI at 1:4,500 (Cat #: D9542; Merck Life Sciences), phalloidin at 1:350 (Cat #: P5282; Merck Life Sciences), and Alexa Fluor 488 anti-rat secondary antibody at 1:400 (Cat #: A21208; Thermo Fisher Scientific).

    Techniques: Control, Transfection, Positive Control, Evaporation, Labeling

    Two-dimensional immunofluorescence stain of isolated bovine muscle satellite cells (BSCs). ( A ) Proliferating bovine satellite stained for DAPI, actin cytoskeleton (Phalloidin), and Pax7, a nuclear marker of satellite cells. Stains show a highly pure satellite cell population, following isolation and pre-plating protocol. ( B ) Following one week of differentiation, cells were stained for DAPI, actin cytoskeleton (Phalloidin), and Troponin T (CT3), a marker of myogenesis. Scale bars are 200 µm.

    Journal: Foods

    Article Title: Extracellular Heme Proteins Influence Bovine Myosatellite Cell Proliferation and the Color of Cell-Based Meat

    doi: 10.3390/foods8100521

    Figure Lengend Snippet: Two-dimensional immunofluorescence stain of isolated bovine muscle satellite cells (BSCs). ( A ) Proliferating bovine satellite stained for DAPI, actin cytoskeleton (Phalloidin), and Pax7, a nuclear marker of satellite cells. Stains show a highly pure satellite cell population, following isolation and pre-plating protocol. ( B ) Following one week of differentiation, cells were stained for DAPI, actin cytoskeleton (Phalloidin), and Troponin T (CT3), a marker of myogenesis. Scale bars are 200 µm.

    Article Snippet: For BAMs, secondary antibodies for Troponin T were diluted in blocking solution containing DAPI (Thermo Fisher, #62248, 1:1000) and added to constructs for 1 h at room temperature.

    Techniques: Immunofluorescence, Staining, Isolation, Marker

    Confocal immunofluorescent imaging of BAMs. BAMs generated from BSC, BSC + Hb, or BSC + Mb (3 mg/mL for both heme proteins) were stained after eight days of differentiation for DAPI, actin cytoskeleton (Phalloidin), and Troponin T (CT3), a marker of myogenesis. Images show multinucleated myotube formation in BSC and BSC + Mb constructs, though not in BSC + Hb constructs. Scale bars are 200 µm.

    Journal: Foods

    Article Title: Extracellular Heme Proteins Influence Bovine Myosatellite Cell Proliferation and the Color of Cell-Based Meat

    doi: 10.3390/foods8100521

    Figure Lengend Snippet: Confocal immunofluorescent imaging of BAMs. BAMs generated from BSC, BSC + Hb, or BSC + Mb (3 mg/mL for both heme proteins) were stained after eight days of differentiation for DAPI, actin cytoskeleton (Phalloidin), and Troponin T (CT3), a marker of myogenesis. Images show multinucleated myotube formation in BSC and BSC + Mb constructs, though not in BSC + Hb constructs. Scale bars are 200 µm.

    Article Snippet: For BAMs, secondary antibodies for Troponin T were diluted in blocking solution containing DAPI (Thermo Fisher, #62248, 1:1000) and added to constructs for 1 h at room temperature.

    Techniques: Imaging, Generated, Staining, Marker, Construct