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fcr blocking reagent, human  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec fcr blocking reagent, human
    Fcr Blocking Reagent, Human, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 3327 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+reagent/FcR+Blocking+Reagent%2C+human/custom%40130-059-901%4042526444
    Average 97 stars, based on 3327 article reviews
    fcr blocking reagent, human - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: A single domain intrabody biases the follicle-stimulating hormone receptor subcellular trafficking
    Article Snippet: Cells were detached following 1 h-incubation in Hanks Balanced Salt Solution (HBSS) w/o Ca2+ and Mg2+ (Eurobio, Les Ulis, France), and re-suspended in working buffer (PBS w/o Ca2+ and Mg2+ , 0.5% BSA) 48 h after transfection. .. Cells were incubated with FcR Blocking Reagent human (1:20, Miltenyi Biotech, Bergisch Gladbach, Germany) for 10 min at 4 °C, and with Phycoerythrin-conjugated anti-FLAG antibody (DYKDDDDK Antibody PE, 1:1000, Miltenyi Biotech, Bergisch Gladbach, Germany) and LIVE/DEAD Fixable Violet Dead Cell Stain (1:1000, Invitrogen, Waltham, MA, USA) for 30 min at 4 °C. .. Cells were washed twice in working buffer before permeabilization using BD Cytofix/Cytoperm Fixation/Permeabilization kit (BD Biosciences, Franklin Lakes, NJ, USA) following manufacturer’s protocol.

    Article Title: Inflammatory macrophages promote metastatic potential in Triple-negative Breast Cancer through Integrated Stress Response signaling
    Article Snippet: Macrophages were detached using StemPro Accutase Cell Dissociation Reagent (Thermo Fisher Scientific) and collected by centrifugation at 300 x g for 10 min. .. Cell pellets were resuspended in FACS buffer consisting of phosphate-buffered saline supplemented with 0,5% Bovine Serum Albumin (BSA; A7906, Sigma Aldrich) and 2mM EDTA (Thermo Fisher Scientific), and incubated with human FcR Blocking Reagent (Miltenyi Biotec) to minimize nonspecific antibody binding. .. Dead cells were excluded using LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (1:100 dilution; Thermo Fisher Scientific).

    Article Title: Activated protein C promotes hematopoietic stem and progenitor cell quiescence and engraftment.
    Article Snippet: Red blood cells (RBCs) were lysed by a 5-min incubation at RT in 1 mL of RBC lysis buffer (0.15 M NH4Cl, 0.01 M Tris-HCl, pH 7.5) and samples washed (300×g, 5 min, 4 °C) and resuspended in 500 μL of FACS buffer supplemented with 1:100 human FcR blocking reagent (Miltenyi Biotech #130-059-901) and 5% normal mouse (Invitrogen #50-112-1171) and 5% normal rat (Invitrogen #10-710-C) serum. .. Red blood cells (RBCs) were lysed by a 5-min incubation at RT in 1 mL of RBC lysis buffer (0.15 M NH4Cl, 0.01 M Tris-HCl, pH 7.5) and samples washed (300×g, 5 min, 4 °C) and resuspended in 500 μL of FACS buffer supplemented with 1:100 human FcR blocking reagent (Miltenyi Biotech #130-059-901) and 5% normal mouse (Invitrogen #50-112-1171) and 5% normal rat (Invitrogen #10-710-C) serum. .. Cells were all acquired at the CytoFLEX LX (Beckman Coulter).

    Blocking Assay:

    Article Title: A single domain intrabody biases the follicle-stimulating hormone receptor subcellular trafficking
    Article Snippet: Cells were detached following 1 h-incubation in Hanks Balanced Salt Solution (HBSS) w/o Ca2+ and Mg2+ (Eurobio, Les Ulis, France), and re-suspended in working buffer (PBS w/o Ca2+ and Mg2+ , 0.5% BSA) 48 h after transfection. .. Cells were incubated with FcR Blocking Reagent human (1:20, Miltenyi Biotech, Bergisch Gladbach, Germany) for 10 min at 4 °C, and with Phycoerythrin-conjugated anti-FLAG antibody (DYKDDDDK Antibody PE, 1:1000, Miltenyi Biotech, Bergisch Gladbach, Germany) and LIVE/DEAD Fixable Violet Dead Cell Stain (1:1000, Invitrogen, Waltham, MA, USA) for 30 min at 4 °C. .. Cells were washed twice in working buffer before permeabilization using BD Cytofix/Cytoperm Fixation/Permeabilization kit (BD Biosciences, Franklin Lakes, NJ, USA) following manufacturer’s protocol.

    Article Title: Inflammatory macrophages promote metastatic potential in Triple-negative Breast Cancer through Integrated Stress Response signaling
    Article Snippet: Macrophages were detached using StemPro Accutase Cell Dissociation Reagent (Thermo Fisher Scientific) and collected by centrifugation at 300 x g for 10 min. .. Cell pellets were resuspended in FACS buffer consisting of phosphate-buffered saline supplemented with 0,5% Bovine Serum Albumin (BSA; A7906, Sigma Aldrich) and 2mM EDTA (Thermo Fisher Scientific), and incubated with human FcR Blocking Reagent (Miltenyi Biotec) to minimize nonspecific antibody binding. .. Dead cells were excluded using LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (1:100 dilution; Thermo Fisher Scientific).

    Article Title: Exploratory analysis of early changes in peripheral immune cell proportions and clinical response following methotrexate and biologic treatment in rheumatoid arthritis
    Article Snippet: .. To prevent nonspecific antibody binding to immune cells, Fc receptors were blocked by incubating the cells with 400 μL human FcR Blocking Reagent (Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany) for 30 minutes at 4°C in the dark. ..

    Article Title: Activated protein C promotes hematopoietic stem and progenitor cell quiescence and engraftment.
    Article Snippet: Red blood cells (RBCs) were lysed by a 5-min incubation at RT in 1 mL of RBC lysis buffer (0.15 M NH4Cl, 0.01 M Tris-HCl, pH 7.5) and samples washed (300×g, 5 min, 4 °C) and resuspended in 500 μL of FACS buffer supplemented with 1:100 human FcR blocking reagent (Miltenyi Biotech #130-059-901) and 5% normal mouse (Invitrogen #50-112-1171) and 5% normal rat (Invitrogen #10-710-C) serum. .. Red blood cells (RBCs) were lysed by a 5-min incubation at RT in 1 mL of RBC lysis buffer (0.15 M NH4Cl, 0.01 M Tris-HCl, pH 7.5) and samples washed (300×g, 5 min, 4 °C) and resuspended in 500 μL of FACS buffer supplemented with 1:100 human FcR blocking reagent (Miltenyi Biotech #130-059-901) and 5% normal mouse (Invitrogen #50-112-1171) and 5% normal rat (Invitrogen #10-710-C) serum. .. Cells were all acquired at the CytoFLEX LX (Beckman Coulter).

    Article Title: A multi-center, single-arm, phase 2 trial of dasatinib in T follicular helper cell lymphomas
    Article Snippet: .. Fc receptor-mediated nonspecific binding was blocked using the FcR Blocking Reagent (Miltenyi Biotec, 130-059-901), and dead cells were excluded by staining with 7- amino-actinomycin D (Thermo Fisher Scientific, A1310). .. FCM sorting and analysis were performed using BD FACSAria III (BD Biosciences), and data were analyzed using the FlowJo software (v10.7.1, Tree Star Inc.).

    Article Title: Synthesis of Antiproliferative N-Chalconyl Imidazolidones and Mechanistic Evaluation of Prototype N-CIMZ 1 as a Macrophage-Modulating Antimitotic Agent
    Article Snippet: .. To prevent non-specific antibody binding via surface Fc receptors and eliminate cellular aggregation, cell pellets were first resuspended in a solution containing 10% human FcR Blocking Reagent (Miltenyi, Paris, France) in PBS supplemented with 0.5% bovine serum albumin (BSA; Thermo Fisher Scientific, Strasbourg, France) and 2 mM EDTA (Thermo Fisher Scientific, Strasbourg, France) which constitutes the staining buffer. ..

    Staining:

    Article Title: A single domain intrabody biases the follicle-stimulating hormone receptor subcellular trafficking
    Article Snippet: Cells were detached following 1 h-incubation in Hanks Balanced Salt Solution (HBSS) w/o Ca2+ and Mg2+ (Eurobio, Les Ulis, France), and re-suspended in working buffer (PBS w/o Ca2+ and Mg2+ , 0.5% BSA) 48 h after transfection. .. Cells were incubated with FcR Blocking Reagent human (1:20, Miltenyi Biotech, Bergisch Gladbach, Germany) for 10 min at 4 °C, and with Phycoerythrin-conjugated anti-FLAG antibody (DYKDDDDK Antibody PE, 1:1000, Miltenyi Biotech, Bergisch Gladbach, Germany) and LIVE/DEAD Fixable Violet Dead Cell Stain (1:1000, Invitrogen, Waltham, MA, USA) for 30 min at 4 °C. .. Cells were washed twice in working buffer before permeabilization using BD Cytofix/Cytoperm Fixation/Permeabilization kit (BD Biosciences, Franklin Lakes, NJ, USA) following manufacturer’s protocol.

    Article Title: A multi-center, single-arm, phase 2 trial of dasatinib in T follicular helper cell lymphomas
    Article Snippet: .. Fc receptor-mediated nonspecific binding was blocked using the FcR Blocking Reagent (Miltenyi Biotec, 130-059-901), and dead cells were excluded by staining with 7- amino-actinomycin D (Thermo Fisher Scientific, A1310). .. FCM sorting and analysis were performed using BD FACSAria III (BD Biosciences), and data were analyzed using the FlowJo software (v10.7.1, Tree Star Inc.).

    Article Title: Synthesis of Antiproliferative N-Chalconyl Imidazolidones and Mechanistic Evaluation of Prototype N-CIMZ 1 as a Macrophage-Modulating Antimitotic Agent
    Article Snippet: .. To prevent non-specific antibody binding via surface Fc receptors and eliminate cellular aggregation, cell pellets were first resuspended in a solution containing 10% human FcR Blocking Reagent (Miltenyi, Paris, France) in PBS supplemented with 0.5% bovine serum albumin (BSA; Thermo Fisher Scientific, Strasbourg, France) and 2 mM EDTA (Thermo Fisher Scientific, Strasbourg, France) which constitutes the staining buffer. ..

    FACS:

    Article Title: Inflammatory macrophages promote metastatic potential in Triple-negative Breast Cancer through Integrated Stress Response signaling
    Article Snippet: Macrophages were detached using StemPro Accutase Cell Dissociation Reagent (Thermo Fisher Scientific) and collected by centrifugation at 300 x g for 10 min. .. Cell pellets were resuspended in FACS buffer consisting of phosphate-buffered saline supplemented with 0,5% Bovine Serum Albumin (BSA; A7906, Sigma Aldrich) and 2mM EDTA (Thermo Fisher Scientific), and incubated with human FcR Blocking Reagent (Miltenyi Biotec) to minimize nonspecific antibody binding. .. Dead cells were excluded using LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (1:100 dilution; Thermo Fisher Scientific).

    Article Title: Activated protein C promotes hematopoietic stem and progenitor cell quiescence and engraftment.
    Article Snippet: Red blood cells (RBCs) were lysed by a 5-min incubation at RT in 1 mL of RBC lysis buffer (0.15 M NH4Cl, 0.01 M Tris-HCl, pH 7.5) and samples washed (300×g, 5 min, 4 °C) and resuspended in 500 μL of FACS buffer supplemented with 1:100 human FcR blocking reagent (Miltenyi Biotech #130-059-901) and 5% normal mouse (Invitrogen #50-112-1171) and 5% normal rat (Invitrogen #10-710-C) serum. .. Red blood cells (RBCs) were lysed by a 5-min incubation at RT in 1 mL of RBC lysis buffer (0.15 M NH4Cl, 0.01 M Tris-HCl, pH 7.5) and samples washed (300×g, 5 min, 4 °C) and resuspended in 500 μL of FACS buffer supplemented with 1:100 human FcR blocking reagent (Miltenyi Biotech #130-059-901) and 5% normal mouse (Invitrogen #50-112-1171) and 5% normal rat (Invitrogen #10-710-C) serum. .. Cells were all acquired at the CytoFLEX LX (Beckman Coulter).

    Saline:

    Article Title: Inflammatory macrophages promote metastatic potential in Triple-negative Breast Cancer through Integrated Stress Response signaling
    Article Snippet: Macrophages were detached using StemPro Accutase Cell Dissociation Reagent (Thermo Fisher Scientific) and collected by centrifugation at 300 x g for 10 min. .. Cell pellets were resuspended in FACS buffer consisting of phosphate-buffered saline supplemented with 0,5% Bovine Serum Albumin (BSA; A7906, Sigma Aldrich) and 2mM EDTA (Thermo Fisher Scientific), and incubated with human FcR Blocking Reagent (Miltenyi Biotec) to minimize nonspecific antibody binding. .. Dead cells were excluded using LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (1:100 dilution; Thermo Fisher Scientific).

    Binding Assay:

    Article Title: Inflammatory macrophages promote metastatic potential in Triple-negative Breast Cancer through Integrated Stress Response signaling
    Article Snippet: Macrophages were detached using StemPro Accutase Cell Dissociation Reagent (Thermo Fisher Scientific) and collected by centrifugation at 300 x g for 10 min. .. Cell pellets were resuspended in FACS buffer consisting of phosphate-buffered saline supplemented with 0,5% Bovine Serum Albumin (BSA; A7906, Sigma Aldrich) and 2mM EDTA (Thermo Fisher Scientific), and incubated with human FcR Blocking Reagent (Miltenyi Biotec) to minimize nonspecific antibody binding. .. Dead cells were excluded using LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (1:100 dilution; Thermo Fisher Scientific).

    Article Title: Exploratory analysis of early changes in peripheral immune cell proportions and clinical response following methotrexate and biologic treatment in rheumatoid arthritis
    Article Snippet: .. To prevent nonspecific antibody binding to immune cells, Fc receptors were blocked by incubating the cells with 400 μL human FcR Blocking Reagent (Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany) for 30 minutes at 4°C in the dark. ..

    Article Title: Synthesis of Antiproliferative N-Chalconyl Imidazolidones and Mechanistic Evaluation of Prototype N-CIMZ 1 as a Macrophage-Modulating Antimitotic Agent
    Article Snippet: .. To prevent non-specific antibody binding via surface Fc receptors and eliminate cellular aggregation, cell pellets were first resuspended in a solution containing 10% human FcR Blocking Reagent (Miltenyi, Paris, France) in PBS supplemented with 0.5% bovine serum albumin (BSA; Thermo Fisher Scientific, Strasbourg, France) and 2 mM EDTA (Thermo Fisher Scientific, Strasbourg, France) which constitutes the staining buffer. ..

    Red Blood Cell Lysis:

    Article Title: Activated protein C promotes hematopoietic stem and progenitor cell quiescence and engraftment.
    Article Snippet: Red blood cells (RBCs) were lysed by a 5-min incubation at RT in 1 mL of RBC lysis buffer (0.15 M NH4Cl, 0.01 M Tris-HCl, pH 7.5) and samples washed (300×g, 5 min, 4 °C) and resuspended in 500 μL of FACS buffer supplemented with 1:100 human FcR blocking reagent (Miltenyi Biotech #130-059-901) and 5% normal mouse (Invitrogen #50-112-1171) and 5% normal rat (Invitrogen #10-710-C) serum. .. Red blood cells (RBCs) were lysed by a 5-min incubation at RT in 1 mL of RBC lysis buffer (0.15 M NH4Cl, 0.01 M Tris-HCl, pH 7.5) and samples washed (300×g, 5 min, 4 °C) and resuspended in 500 μL of FACS buffer supplemented with 1:100 human FcR blocking reagent (Miltenyi Biotech #130-059-901) and 5% normal mouse (Invitrogen #50-112-1171) and 5% normal rat (Invitrogen #10-710-C) serum. .. Cells were all acquired at the CytoFLEX LX (Beckman Coulter).

    Nonspecific Binding:

    Article Title: A multi-center, single-arm, phase 2 trial of dasatinib in T follicular helper cell lymphomas
    Article Snippet: .. Fc receptor-mediated nonspecific binding was blocked using the FcR Blocking Reagent (Miltenyi Biotec, 130-059-901), and dead cells were excluded by staining with 7- amino-actinomycin D (Thermo Fisher Scientific, A1310). .. FCM sorting and analysis were performed using BD FACSAria III (BD Biosciences), and data were analyzed using the FlowJo software (v10.7.1, Tree Star Inc.).



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