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fcr blocking reagent, human  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec fcr blocking reagent, human
    Fcr Blocking Reagent, Human, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 3312 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+reagent/FcR+Blocking+Reagent%2C+human/custom%40130-059-901%4042409814
    Average 97 stars, based on 3312 article reviews
    fcr blocking reagent, human - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Genomic profiling identifies actionable DNA-repair defects in a new cervical cancer model
    Article Snippet: .. Cells were treated with an FcR blocking reagent (130-059-901, Miltenyi Biotec) for 10 min at 4 °C prior to antibody staining. ..

    Article Title: Human lungs maintain tissue-resident memory T cells against a broad spectrum of pathogens.
    Article Snippet: .. The resulting cell suspensions (across all sample types) were centrifuged, and the pellet was resuspended in MACS buffer containing human FcR Blocking Reagent (Miltenyi, 130059901), followed by addition of Cell-hashtag TotalSeq-C antibody (1 μg per condition) to enable hashtag-oligonucleotide-based sample deconvolution at the analysis stage. ..

    Article Title: A novel Vδ1 engager targeting CD19 enhances human Vδ1 γδ T cell responses against CLL and CD19+ hematological malignancies.
    Article Snippet: γδ T cells are associated with favorable outcomes in many cancers likely through a mechanism of stress-directed cytotoxicity and antitumor cytokine production.. Vδ1 γδ T cells are especially promising for immunotherapy due to their broad stress recognition and resistance to activation-induced cell death.. Here, we generate a CD19 engager incorporating a novel Vδ1 binding moiety as proof-of-concept for treating CD19 cancers, including Chronic Lymphocytic Leukemia (CLL).

    Article Title: Carboxyamidotriazole treatment correlates with dynamic changes in macrophage metabolism and function in models of colitis and colitis-associated carcinogenesis.
    Article Snippet: Carboxyamidotriazole (CAI), an inhibitor of mitochondrial complex I, demonstrates anti-inflammatory and antitumor properties.. While effective against colitis, its potential link to macrophage metabolism in colitis and colitis-associated colorectal cancer (CAC) remained unexplored.. This study investigates the role of CAI and its association with macrophage metabolic states in these conditions.

    Article Title: Distinct systemic and gut IgA responses to bacteria of the human upper gastrointestinal tract.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC-mouse anti-human CD38 Thermo Fisher Cat# 11-0388-42, RRID:AB_10547895 APC-mouse anti-human IgA Miltenyi Cat# 130-113-472, RRID:AB_2733422 PerCP/Cy5.5-mouse anti-human IgD BioLegend Cat# 348208, RRID:AB_10641706 BV510-mouse anti-human CD3 BioLegend Cat# 317331, RRID:AB_2561376 BV510-mouse anti-human CD14 BioLegend Cat# 301842, RRID:AB_2561946 AF647-goat F(ab’)2 anti-human IgA SouthernBiotech Cat# 2052-31, RRID:AB_2795711 Goat anti-human Ig SouthernBiotech Cat# 2010-01, RRID:AB_2687525 AP-goat anti-human IgA Sigma Cat# A9669, RRID:AB_258467 AP-goat anti-human IgG SouthernBiotech Cat# 2040-04, RRID:AB_2795643 AP-goat anti-human IgM SouthernBiotech Cat# 2020-04, RRID:AB_2795602 Rabbit anti-human IgA Agilent Cat# A0262, RRID:AB_3094763 HRP-goat anti-rabbit Ig SouthernBiotech Cat# 4010-05, RRID:AB_2632593 Barcoded mouse anti-human CD27, TotalSeq-C0154 BioLegend Cat# 302853, RRID:AB_2800747 Barcoded mouse anti-human hashtag 1, TotalSeq-C0251 BioLegend Cat# 394661, RRID:AB_2801031 Barcoded mouse anti-human hashtag 2, TotalSeq-C0252 BioLegend Cat# 394663, RRID:AB_2801032 Biological samples Blood samples of patients with celiac disease and controls This paper N/A Duodenal biopsies This paper N/A Bone marrow aspirates This paper N/A Chemicals, peptides, and recombinant proteins Streptavidin-PE Thermo Fisher Cat# S866 Streptavidin-PE/Cy7 Thermo Fisher Cat# SA1012 Barcoded streptavidin-PE, TotalSeq-C0951 BioLegend Cat# 405261 FcR Blocking Reagent Miltenyi Cat# 130-059-901 LIVE/DEAD fixable Aqua Thermo Fisher Cat# L34957 LIVE/DEAD fixable Near-IR Thermo Fisher Cat# L10119 4% formaldehyde fixative solution Thermo Fisher Cat# FB002 Calf thymus DNA Thermo Fisher Cat# 15633019 Keyhole limpet hemocyanin Sigma Cat# H8283 Human insulin Sigma Cat# I9278 LPS from E. coli O55:B5 Sigma Cat# L2637 LPS from E. coli O111:B4 Sigma Cat# L3012 LPS from K. pneumoniae Sigma Cat# L4268 Ultrapure LPS from E. coli O55:B5 InvivoGen Cat# tlrl-pb5lps Recombinant Lpp from E. coli TargetMol Cat# TMPH-00651 Mutanolysin Sigma Cat# M9901 Proteinase K Qiagen Cat# 69504 Biotin-TG2 Lindeman et al.29 N/A Biotin-GST Lindeman et al.29 N/A (Continued on next page) 16 Cell Reports 45, 117721, August 25, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant anti-TG2 IgA1 mAb Iversen et al.89 N/A Recombinant polyreactive IgG1 mAb Das et al.90 N/A Recombinant IgA1 mAbs cloned from human plasma cells This paper N/A Critical commercial assays AnaeroGen sachets Thermo Fisher Cat# AN0025A Peptide M-agarose InvivoGen Cat# gel-pdm-2 HiTrap protein G columns Cytiva Cat# 29048581 MultiScreen 96 well plates Sigma Cat# MSIPS4510 4-20% Tris-glycine gels Thermo Fisher Cat# XP04200BOX West Pico PLUS substrate Thermo Fisher Cat# 34580 BCIP/NBT substrate Bio-Rad Cat# 1706432 PageBlue protein staining solution Thermo Fisher Cat# 24620 Chromium Single Cell 5′ Library & Gel Bead Kit 10x Genomics Cat# 1000014 Chromium Single Cell 5′ Library Construction Kit 10x Genomics Cat# 1000020 Chromium Chip A Single Cell Kit 10x Genomics Cat# 1000009 Chromium i7 Multiplex Kit 10x Genomics Cat# 120262 Chromium i7 Multiplex Kit N, Set A 10x Genomics Cat# 1000084 Chromium Single Cell 5′ Feature Barcode Library Kit 10x Genomics Cat# 1000080 Chromium Single Cell V(D)J Enrichment Kit, Human B cells 10x Genomics Cat# 1000016 Deposited data scRNA-seq and V(D)J sequences of gut and BM plasma cells This study Federated European Genome-Phenome Archive: EGAS50000001912 Experimental models: strains Bacterial isolates from duodenal biopsies This paper N/A Control strains See Table S3 See Table S3 Oligonucleotides Immunoglobulin primers Tiller et al.88 N/A BlpI-IGHA reverse primer CAGAGGCTCAGCGGGAAGACCTTG Eurogentec N/A Recombinant DNA Heavy chain V regions in AbVec-hIgA1 This study N/A Kappa light chain V regions in AbVechIgKappa This study N/A Lambda light chain V regions in AbVechIgLambda This study N/A Software and algorithms FlowJo v10 BD https://www.flowjo.com/ GraphPad Prism v10 Dotmatrics https://www.graphpad.com/ R v.4.4.3 The R Foundation https://www.r-project.org ggpubr v0.6.0 N/A https://cran.r-project.org/web/packages/ ggpubr/index.html Cell Ranger v6.0.2 10x Genomics https://www.10xgenomics.com/support/ software/cell-ranger/latest Seurat v5.2.1 Hao et al. 2021 https://satijalab.org/seurat/ EnhancedVolcano v1.18.0 N/A https://github.com/kevinblighe/ EnhancedVolcano (Continued on next page) Cell Reports 45, 117721, August 25, 2026 17

    Staining:

    Article Title: Genomic profiling identifies actionable DNA-repair defects in a new cervical cancer model
    Article Snippet: .. Cells were treated with an FcR blocking reagent (130-059-901, Miltenyi Biotec) for 10 min at 4 °C prior to antibody staining. ..

    Article Title: A novel Vδ1 engager targeting CD19 enhances human Vδ1 γδ T cell responses against CLL and CD19+ hematological malignancies.
    Article Snippet: γδ T cells are associated with favorable outcomes in many cancers likely through a mechanism of stress-directed cytotoxicity and antitumor cytokine production.. Vδ1 γδ T cells are especially promising for immunotherapy due to their broad stress recognition and resistance to activation-induced cell death.. Here, we generate a CD19 engager incorporating a novel Vδ1 binding moiety as proof-of-concept for treating CD19 cancers, including Chronic Lymphocytic Leukemia (CLL).

    Magnetic Cell Separation:

    Article Title: Human lungs maintain tissue-resident memory T cells against a broad spectrum of pathogens.
    Article Snippet: .. The resulting cell suspensions (across all sample types) were centrifuged, and the pellet was resuspended in MACS buffer containing human FcR Blocking Reagent (Miltenyi, 130059901), followed by addition of Cell-hashtag TotalSeq-C antibody (1 μg per condition) to enable hashtag-oligonucleotide-based sample deconvolution at the analysis stage. ..

    Marker:

    Article Title: A novel Vδ1 engager targeting CD19 enhances human Vδ1 γδ T cell responses against CLL and CD19+ hematological malignancies.
    Article Snippet: γδ T cells are associated with favorable outcomes in many cancers likely through a mechanism of stress-directed cytotoxicity and antitumor cytokine production.. Vδ1 γδ T cells are especially promising for immunotherapy due to their broad stress recognition and resistance to activation-induced cell death.. Here, we generate a CD19 engager incorporating a novel Vδ1 binding moiety as proof-of-concept for treating CD19 cancers, including Chronic Lymphocytic Leukemia (CLL).

    Incubation:

    Article Title: Carboxyamidotriazole treatment correlates with dynamic changes in macrophage metabolism and function in models of colitis and colitis-associated carcinogenesis.
    Article Snippet: Carboxyamidotriazole (CAI), an inhibitor of mitochondrial complex I, demonstrates anti-inflammatory and antitumor properties.. While effective against colitis, its potential link to macrophage metabolism in colitis and colitis-associated colorectal cancer (CAC) remained unexplored.. This study investigates the role of CAI and its association with macrophage metabolic states in these conditions.

    Sedimentation:

    Article Title: Carboxyamidotriazole treatment correlates with dynamic changes in macrophage metabolism and function in models of colitis and colitis-associated carcinogenesis.
    Article Snippet: Carboxyamidotriazole (CAI), an inhibitor of mitochondrial complex I, demonstrates anti-inflammatory and antitumor properties.. While effective against colitis, its potential link to macrophage metabolism in colitis and colitis-associated colorectal cancer (CAC) remained unexplored.. This study investigates the role of CAI and its association with macrophage metabolic states in these conditions.

    Recombinant:

    Article Title: Distinct systemic and gut IgA responses to bacteria of the human upper gastrointestinal tract.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC-mouse anti-human CD38 Thermo Fisher Cat# 11-0388-42, RRID:AB_10547895 APC-mouse anti-human IgA Miltenyi Cat# 130-113-472, RRID:AB_2733422 PerCP/Cy5.5-mouse anti-human IgD BioLegend Cat# 348208, RRID:AB_10641706 BV510-mouse anti-human CD3 BioLegend Cat# 317331, RRID:AB_2561376 BV510-mouse anti-human CD14 BioLegend Cat# 301842, RRID:AB_2561946 AF647-goat F(ab’)2 anti-human IgA SouthernBiotech Cat# 2052-31, RRID:AB_2795711 Goat anti-human Ig SouthernBiotech Cat# 2010-01, RRID:AB_2687525 AP-goat anti-human IgA Sigma Cat# A9669, RRID:AB_258467 AP-goat anti-human IgG SouthernBiotech Cat# 2040-04, RRID:AB_2795643 AP-goat anti-human IgM SouthernBiotech Cat# 2020-04, RRID:AB_2795602 Rabbit anti-human IgA Agilent Cat# A0262, RRID:AB_3094763 HRP-goat anti-rabbit Ig SouthernBiotech Cat# 4010-05, RRID:AB_2632593 Barcoded mouse anti-human CD27, TotalSeq-C0154 BioLegend Cat# 302853, RRID:AB_2800747 Barcoded mouse anti-human hashtag 1, TotalSeq-C0251 BioLegend Cat# 394661, RRID:AB_2801031 Barcoded mouse anti-human hashtag 2, TotalSeq-C0252 BioLegend Cat# 394663, RRID:AB_2801032 Biological samples Blood samples of patients with celiac disease and controls This paper N/A Duodenal biopsies This paper N/A Bone marrow aspirates This paper N/A Chemicals, peptides, and recombinant proteins Streptavidin-PE Thermo Fisher Cat# S866 Streptavidin-PE/Cy7 Thermo Fisher Cat# SA1012 Barcoded streptavidin-PE, TotalSeq-C0951 BioLegend Cat# 405261 FcR Blocking Reagent Miltenyi Cat# 130-059-901 LIVE/DEAD fixable Aqua Thermo Fisher Cat# L34957 LIVE/DEAD fixable Near-IR Thermo Fisher Cat# L10119 4% formaldehyde fixative solution Thermo Fisher Cat# FB002 Calf thymus DNA Thermo Fisher Cat# 15633019 Keyhole limpet hemocyanin Sigma Cat# H8283 Human insulin Sigma Cat# I9278 LPS from E. coli O55:B5 Sigma Cat# L2637 LPS from E. coli O111:B4 Sigma Cat# L3012 LPS from K. pneumoniae Sigma Cat# L4268 Ultrapure LPS from E. coli O55:B5 InvivoGen Cat# tlrl-pb5lps Recombinant Lpp from E. coli TargetMol Cat# TMPH-00651 Mutanolysin Sigma Cat# M9901 Proteinase K Qiagen Cat# 69504 Biotin-TG2 Lindeman et al.29 N/A Biotin-GST Lindeman et al.29 N/A (Continued on next page) 16 Cell Reports 45, 117721, August 25, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant anti-TG2 IgA1 mAb Iversen et al.89 N/A Recombinant polyreactive IgG1 mAb Das et al.90 N/A Recombinant IgA1 mAbs cloned from human plasma cells This paper N/A Critical commercial assays AnaeroGen sachets Thermo Fisher Cat# AN0025A Peptide M-agarose InvivoGen Cat# gel-pdm-2 HiTrap protein G columns Cytiva Cat# 29048581 MultiScreen 96 well plates Sigma Cat# MSIPS4510 4-20% Tris-glycine gels Thermo Fisher Cat# XP04200BOX West Pico PLUS substrate Thermo Fisher Cat# 34580 BCIP/NBT substrate Bio-Rad Cat# 1706432 PageBlue protein staining solution Thermo Fisher Cat# 24620 Chromium Single Cell 5′ Library & Gel Bead Kit 10x Genomics Cat# 1000014 Chromium Single Cell 5′ Library Construction Kit 10x Genomics Cat# 1000020 Chromium Chip A Single Cell Kit 10x Genomics Cat# 1000009 Chromium i7 Multiplex Kit 10x Genomics Cat# 120262 Chromium i7 Multiplex Kit N, Set A 10x Genomics Cat# 1000084 Chromium Single Cell 5′ Feature Barcode Library Kit 10x Genomics Cat# 1000080 Chromium Single Cell V(D)J Enrichment Kit, Human B cells 10x Genomics Cat# 1000016 Deposited data scRNA-seq and V(D)J sequences of gut and BM plasma cells This study Federated European Genome-Phenome Archive: EGAS50000001912 Experimental models: strains Bacterial isolates from duodenal biopsies This paper N/A Control strains See Table S3 See Table S3 Oligonucleotides Immunoglobulin primers Tiller et al.88 N/A BlpI-IGHA reverse primer CAGAGGCTCAGCGGGAAGACCTTG Eurogentec N/A Recombinant DNA Heavy chain V regions in AbVec-hIgA1 This study N/A Kappa light chain V regions in AbVechIgKappa This study N/A Lambda light chain V regions in AbVechIgLambda This study N/A Software and algorithms FlowJo v10 BD https://www.flowjo.com/ GraphPad Prism v10 Dotmatrics https://www.graphpad.com/ R v.4.4.3 The R Foundation https://www.r-project.org ggpubr v0.6.0 N/A https://cran.r-project.org/web/packages/ ggpubr/index.html Cell Ranger v6.0.2 10x Genomics https://www.10xgenomics.com/support/ software/cell-ranger/latest Seurat v5.2.1 Hao et al. 2021 https://satijalab.org/seurat/ EnhancedVolcano v1.18.0 N/A https://github.com/kevinblighe/ EnhancedVolcano (Continued on next page) Cell Reports 45, 117721, August 25, 2026 17



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