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nuclear extraction kit  (OriGene)


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    Structured Review

    OriGene nuclear extraction kit
    Nuclear Extraction Kit, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+kit/Protein+Block+Kit/pmc06110824-80-6-9
    Average 94 stars, based on 6 article reviews
    nuclear extraction kit - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Immunohistochemical staining:

    Article Title: Single-cell analysis reveals transcriptomic and epigenomic impacts on the maternal-fetal interface following SARS-CoV-2 infection.
    Article Snippet: .. For the immunohistochemical staining, the slides were treated with 3% hydrogen peroxide, followed by heating in antigen retrieval buffer (pH 6.0; Abcam), incubation with Protein Block (Abcam) and overnight incubation at 4 °C with primary antibodies to FLNB (ab282106, Abcam), PAPPA (ab174314, Abcam) and PSG9 (AP53483PU-N, Origene). .. After washing with 0.1% TBS in Tween-20, the slides were incubated with the secondary antibody horseradish peroxidase (HRP)-conjugated mouse anti-rabbit (Sigma-Aldrich), stained with the substrate 3,3′-diaminobenzidine (EnVision) and counterstained with haematoxylin (Sigma-Aldrich).

    Staining:

    Article Title: Single-cell analysis reveals transcriptomic and epigenomic impacts on the maternal-fetal interface following SARS-CoV-2 infection.
    Article Snippet: .. For the immunohistochemical staining, the slides were treated with 3% hydrogen peroxide, followed by heating in antigen retrieval buffer (pH 6.0; Abcam), incubation with Protein Block (Abcam) and overnight incubation at 4 °C with primary antibodies to FLNB (ab282106, Abcam), PAPPA (ab174314, Abcam) and PSG9 (AP53483PU-N, Origene). .. After washing with 0.1% TBS in Tween-20, the slides were incubated with the secondary antibody horseradish peroxidase (HRP)-conjugated mouse anti-rabbit (Sigma-Aldrich), stained with the substrate 3,3′-diaminobenzidine (EnVision) and counterstained with haematoxylin (Sigma-Aldrich).

    Incubation:

    Article Title: Single-cell analysis reveals transcriptomic and epigenomic impacts on the maternal-fetal interface following SARS-CoV-2 infection.
    Article Snippet: .. For the immunohistochemical staining, the slides were treated with 3% hydrogen peroxide, followed by heating in antigen retrieval buffer (pH 6.0; Abcam), incubation with Protein Block (Abcam) and overnight incubation at 4 °C with primary antibodies to FLNB (ab282106, Abcam), PAPPA (ab174314, Abcam) and PSG9 (AP53483PU-N, Origene). .. After washing with 0.1% TBS in Tween-20, the slides were incubated with the secondary antibody horseradish peroxidase (HRP)-conjugated mouse anti-rabbit (Sigma-Aldrich), stained with the substrate 3,3′-diaminobenzidine (EnVision) and counterstained with haematoxylin (Sigma-Aldrich).

    Article Title: Pathogenesis of multiple lentigines in LEOPARD syndrome with PTPN11 gene mutation.
    Article Snippet: .. After blocking using Peroxidase Blocking (Dako, Copenhagen, Denmark) for 5 min, and Protein Block (Dako) for 10 min, sections were incubated with antiMelan-A antibody (Abcam, Cambridge, UK), anti-endothelin-1 antibody (Abcam), anti-αMSH (Acris Antibodies, San Diego, CA, USA), anti-SCF antibody (Abcam), anti-phospho-Akt (Ser473) antibody, anti-phospho-mTOR (Ser2448) antibody (Cell Signaling, Danvers, MA, USA), or anti-phospho-STAT-3 (Ser727) antibody (Millipore, Billerica, MA, USA) overnight at 4°C. .. After washing, the sections were incubated with a horseradish peroxidase-labelled polymer-conjugated anti-mouse secondary antibody (ENVISION+; Dako) for 1 h at room temperature.

    Blocking Assay:

    Article Title: Single-cell analysis reveals transcriptomic and epigenomic impacts on the maternal-fetal interface following SARS-CoV-2 infection.
    Article Snippet: .. For the immunohistochemical staining, the slides were treated with 3% hydrogen peroxide, followed by heating in antigen retrieval buffer (pH 6.0; Abcam), incubation with Protein Block (Abcam) and overnight incubation at 4 °C with primary antibodies to FLNB (ab282106, Abcam), PAPPA (ab174314, Abcam) and PSG9 (AP53483PU-N, Origene). .. After washing with 0.1% TBS in Tween-20, the slides were incubated with the secondary antibody horseradish peroxidase (HRP)-conjugated mouse anti-rabbit (Sigma-Aldrich), stained with the substrate 3,3′-diaminobenzidine (EnVision) and counterstained with haematoxylin (Sigma-Aldrich).

    Article Title: Pathogenesis of multiple lentigines in LEOPARD syndrome with PTPN11 gene mutation.
    Article Snippet: .. After blocking using Peroxidase Blocking (Dako, Copenhagen, Denmark) for 5 min, and Protein Block (Dako) for 10 min, sections were incubated with antiMelan-A antibody (Abcam, Cambridge, UK), anti-endothelin-1 antibody (Abcam), anti-αMSH (Acris Antibodies, San Diego, CA, USA), anti-SCF antibody (Abcam), anti-phospho-Akt (Ser473) antibody, anti-phospho-mTOR (Ser2448) antibody (Cell Signaling, Danvers, MA, USA), or anti-phospho-STAT-3 (Ser727) antibody (Millipore, Billerica, MA, USA) overnight at 4°C. .. After washing, the sections were incubated with a horseradish peroxidase-labelled polymer-conjugated anti-mouse secondary antibody (ENVISION+; Dako) for 1 h at room temperature.

    Article Title: Mesenchymal stromal cell therapy is a long-term regenerative approach for chronic radiation cystitis, involving coordinated modulation of the urothelial, immune, vascular, and fibrotic pathways
    Article Snippet: .. A pretreatment method using heat-induced epitope retrieval was performed and the non-specific binding was blocked using Protein Block Solution (Origene, E17-18). .. Umbrella cells were labelled using a mouse anti-uroplakin 3 (UPK3) antibody (Abcam ab78196, 1:500 dilution).

    Binding Assay:

    Article Title: Mesenchymal stromal cell therapy is a long-term regenerative approach for chronic radiation cystitis, involving coordinated modulation of the urothelial, immune, vascular, and fibrotic pathways
    Article Snippet: .. A pretreatment method using heat-induced epitope retrieval was performed and the non-specific binding was blocked using Protein Block Solution (Origene, E17-18). .. Umbrella cells were labelled using a mouse anti-uroplakin 3 (UPK3) antibody (Abcam ab78196, 1:500 dilution).

    Purification:

    Article Title: Ethanol and C2 ceramide activate fatty acid oxidation in human hepatoma cells
    Article Snippet: .. VL-17A nuclei were purified using the Nuclear Extraction Kit (Origene, Rockville, MD) and nuclear protein was quantified by BCA assay. .. PPARα DNA-binding in 7 μg of nuclear protein was measured using the Transcription Factor Assay Kit (Cayman Chemical) according to manufacturer’s instructions.

    Extraction:

    Article Title: Ethanol and C2 ceramide activate fatty acid oxidation in human hepatoma cells
    Article Snippet: .. VL-17A nuclei were purified using the Nuclear Extraction Kit (Origene, Rockville, MD) and nuclear protein was quantified by BCA assay. .. PPARα DNA-binding in 7 μg of nuclear protein was measured using the Transcription Factor Assay Kit (Cayman Chemical) according to manufacturer’s instructions.

    BIA-KA:

    Article Title: Ethanol and C2 ceramide activate fatty acid oxidation in human hepatoma cells
    Article Snippet: .. VL-17A nuclei were purified using the Nuclear Extraction Kit (Origene, Rockville, MD) and nuclear protein was quantified by BCA assay. .. PPARα DNA-binding in 7 μg of nuclear protein was measured using the Transcription Factor Assay Kit (Cayman Chemical) according to manufacturer’s instructions.



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