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Bethyl diluent
Diluent, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blocking+buffer/ELISA+Blocking+Buffer/us08674115-1364-7-22
Average 93 stars, based on 36 article reviews
diluent - by Bioz Stars, 2026-09
93/100 stars

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Blocking Assay:

Article Title: Modulatory Effects of M3 Muscarinic Acetylcholine Receptor on Inflammatory Profiles of Human Memory T Helper Cells
Article Snippet: IFN-γ, IL-4 (BD Biosciences, USA), and IL-17A were measured from memory Th cell supernatants using commercially available sandwich ELISA kits according to manufacturer protocols (ThermoFisher Scientific, Burlington, Canada). .. Modifications included 75μL of coating and capture antibody per well, and blocking with ELISA blocking buffer powder (Bethyl Laboratories Inc. from Cedarlane) reconstituted in milliQ H 2 O. TMB substrate for the IL-4 and IFN-γ was ELISA Ultrasensitive TMB Substrate for HRP (Applied Biological Mat.Inc. ..

Article Title: Taming immune responses to AAV gene therapy by programmed in vivo Treg expansion.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Article Title: High-sensitivity in situ capture of endogenous RNA-protein interactions in fixed cells and primary tissues
Article Snippet: .. Following 3 cycles of washing in 300 μL Washing Buffer, cells were incubated with 100 μL blocking buffer (5% BSA in Washing Buffer with 0.2U/μL RNaseOUT) for 1 h. After blocking, the cells were incubated in 150 μL diluted primary antibodies (rabbit anti-RBFOX2, Bethyl, A300-864A; rabbit anti-TDP-43, Bethyl, A303-223A) with optimized concentration in Antibody Dilution Buffer (PBS with 1% BSA, 2 mM DTT and 0.2U/μL RNaseOUT) for 1 h. After 3 cycles of washing in PBS-T, 100 μL 1 μg/mL APO1-Nb in dilution buffer was added for 1-h incubation followed by 6 times of washing in PBS-T. ..

Article Title: Arginine metabolism has a pivotal function for the encystation of Giardia duodenalis
Article Snippet: .. After 3 washing steps with blocking buffer for 10 minutes each, secondary antibodies (goat anti lama DyLight650, 1:300; Bethyl laboratories) and directly labeled antibody Giardi-a-Glo (FITC) were diluted in blocking buffer and incubated for 2 hours at room temperature. ..

Article Title: Human Fcγ-receptors selectively respond to C-reactive protein isoforms
Article Snippet: .. Following the same blocking and washing steps as described above, IgG1, pCRP, or mCRP were added in 50 μl ELISA blocking buffer, and binding was detected using goat anti-hCRP antibody (1:3,000; 1 mg/ml; Bethyl: A80-125A) and donkey anti-goat (DAG) POD-conjugated antibody (DAG-POD; 1:5,000; 2,5mg/ml; Invitrogen, Waltham, Massachusetts, USA: A16005) for CRP (pCRP and mCRP) and goat anti-human-IgG-POD (1:3,000, 1 mg/ml; Rockland Immunochemical, Philadelphia, Pennsylvania, USA, #109-035-003) for IgG1. ..

Article Title: Human Fcγ-receptors selectively respond to C-reactive protein (CRP) isoforms
Article Snippet: .. Following the same blocking and washing steps as described above, IgG1, pCRP, or mCRP were added in 50 μl ELISA blocking buffer, and binding was detected using goat anti-hCRP antibody (1:3,000; 1 mg/ml; Bethyl: A80-125A) and donkey anti-goat (DAG) POD-conjugated antibody (DAG-POD; 1:5,000; 2,5mg/ml; Invitrogen, Waltham, Massachusetts, USA: A16005) for CRP (pCRP and mCRP) and goat anti-human-IgG-POD (1:3,000, 1 mg/ml; Rockland Immunochemical, Philadelphia, Pennsylvania, USA,, #109-035-003) for IgG1. ..

Article Title: Modulatory effects of M3 muscarinic acetylcholine receptor on inflammatory profiles of human memory T helper cells.
Article Snippet: IFN-γ (Thermo Fisher Scientific, Burlington, Canada), IL-4 (BD Biosciences, United States of America), and IL-17A (Thermo Fisher Scientific, Burlington, Canada) were measured from memory Th-cell supernatants using commercially available sandwich ELISA kits according to manufacturer protocols. .. Modifications included 75 μL of coating and capture antibody per well, and blocking with ELISA blocking buffer powder (Bethyl Laboratories Inc. from Cedarlane) reconstituted in milli-Q H2O. ..

Enzyme-linked Immunosorbent Assay:

Article Title: Modulatory Effects of M3 Muscarinic Acetylcholine Receptor on Inflammatory Profiles of Human Memory T Helper Cells
Article Snippet: IFN-γ, IL-4 (BD Biosciences, USA), and IL-17A were measured from memory Th cell supernatants using commercially available sandwich ELISA kits according to manufacturer protocols (ThermoFisher Scientific, Burlington, Canada). .. Modifications included 75μL of coating and capture antibody per well, and blocking with ELISA blocking buffer powder (Bethyl Laboratories Inc. from Cedarlane) reconstituted in milliQ H 2 O. TMB substrate for the IL-4 and IFN-γ was ELISA Ultrasensitive TMB Substrate for HRP (Applied Biological Mat.Inc. ..

Article Title: Taming immune responses to AAV gene therapy by programmed in vivo Treg expansion.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Article Title: Human Fcγ-receptors selectively respond to C-reactive protein isoforms
Article Snippet: .. Following the same blocking and washing steps as described above, IgG1, pCRP, or mCRP were added in 50 μl ELISA blocking buffer, and binding was detected using goat anti-hCRP antibody (1:3,000; 1 mg/ml; Bethyl: A80-125A) and donkey anti-goat (DAG) POD-conjugated antibody (DAG-POD; 1:5,000; 2,5mg/ml; Invitrogen, Waltham, Massachusetts, USA: A16005) for CRP (pCRP and mCRP) and goat anti-human-IgG-POD (1:3,000, 1 mg/ml; Rockland Immunochemical, Philadelphia, Pennsylvania, USA, #109-035-003) for IgG1. ..

Article Title: Human Fcγ-receptors selectively respond to C-reactive protein (CRP) isoforms
Article Snippet: .. Following the same blocking and washing steps as described above, IgG1, pCRP, or mCRP were added in 50 μl ELISA blocking buffer, and binding was detected using goat anti-hCRP antibody (1:3,000; 1 mg/ml; Bethyl: A80-125A) and donkey anti-goat (DAG) POD-conjugated antibody (DAG-POD; 1:5,000; 2,5mg/ml; Invitrogen, Waltham, Massachusetts, USA: A16005) for CRP (pCRP and mCRP) and goat anti-human-IgG-POD (1:3,000, 1 mg/ml; Rockland Immunochemical, Philadelphia, Pennsylvania, USA,, #109-035-003) for IgG1. ..

Article Title: Modulatory effects of M3 muscarinic acetylcholine receptor on inflammatory profiles of human memory T helper cells.
Article Snippet: IFN-γ (Thermo Fisher Scientific, Burlington, Canada), IL-4 (BD Biosciences, United States of America), and IL-17A (Thermo Fisher Scientific, Burlington, Canada) were measured from memory Th-cell supernatants using commercially available sandwich ELISA kits according to manufacturer protocols. .. Modifications included 75 μL of coating and capture antibody per well, and blocking with ELISA blocking buffer powder (Bethyl Laboratories Inc. from Cedarlane) reconstituted in milli-Q H2O. ..

Incubation:

Article Title: High-sensitivity in situ capture of endogenous RNA-protein interactions in fixed cells and primary tissues
Article Snippet: .. Following 3 cycles of washing in 300 μL Washing Buffer, cells were incubated with 100 μL blocking buffer (5% BSA in Washing Buffer with 0.2U/μL RNaseOUT) for 1 h. After blocking, the cells were incubated in 150 μL diluted primary antibodies (rabbit anti-RBFOX2, Bethyl, A300-864A; rabbit anti-TDP-43, Bethyl, A303-223A) with optimized concentration in Antibody Dilution Buffer (PBS with 1% BSA, 2 mM DTT and 0.2U/μL RNaseOUT) for 1 h. After 3 cycles of washing in PBS-T, 100 μL 1 μg/mL APO1-Nb in dilution buffer was added for 1-h incubation followed by 6 times of washing in PBS-T. ..

Article Title: Arginine metabolism has a pivotal function for the encystation of Giardia duodenalis
Article Snippet: .. After 3 washing steps with blocking buffer for 10 minutes each, secondary antibodies (goat anti lama DyLight650, 1:300; Bethyl laboratories) and directly labeled antibody Giardi-a-Glo (FITC) were diluted in blocking buffer and incubated for 2 hours at room temperature. ..

Concentration Assay:

Article Title: High-sensitivity in situ capture of endogenous RNA-protein interactions in fixed cells and primary tissues
Article Snippet: .. Following 3 cycles of washing in 300 μL Washing Buffer, cells were incubated with 100 μL blocking buffer (5% BSA in Washing Buffer with 0.2U/μL RNaseOUT) for 1 h. After blocking, the cells were incubated in 150 μL diluted primary antibodies (rabbit anti-RBFOX2, Bethyl, A300-864A; rabbit anti-TDP-43, Bethyl, A303-223A) with optimized concentration in Antibody Dilution Buffer (PBS with 1% BSA, 2 mM DTT and 0.2U/μL RNaseOUT) for 1 h. After 3 cycles of washing in PBS-T, 100 μL 1 μg/mL APO1-Nb in dilution buffer was added for 1-h incubation followed by 6 times of washing in PBS-T. ..

Labeling:

Article Title: Arginine metabolism has a pivotal function for the encystation of Giardia duodenalis
Article Snippet: .. After 3 washing steps with blocking buffer for 10 minutes each, secondary antibodies (goat anti lama DyLight650, 1:300; Bethyl laboratories) and directly labeled antibody Giardi-a-Glo (FITC) were diluted in blocking buffer and incubated for 2 hours at room temperature. ..

Binding Assay:

Article Title: Human Fcγ-receptors selectively respond to C-reactive protein isoforms
Article Snippet: .. Following the same blocking and washing steps as described above, IgG1, pCRP, or mCRP were added in 50 μl ELISA blocking buffer, and binding was detected using goat anti-hCRP antibody (1:3,000; 1 mg/ml; Bethyl: A80-125A) and donkey anti-goat (DAG) POD-conjugated antibody (DAG-POD; 1:5,000; 2,5mg/ml; Invitrogen, Waltham, Massachusetts, USA: A16005) for CRP (pCRP and mCRP) and goat anti-human-IgG-POD (1:3,000, 1 mg/ml; Rockland Immunochemical, Philadelphia, Pennsylvania, USA, #109-035-003) for IgG1. ..

Article Title: Human Fcγ-receptors selectively respond to C-reactive protein (CRP) isoforms
Article Snippet: .. Following the same blocking and washing steps as described above, IgG1, pCRP, or mCRP were added in 50 μl ELISA blocking buffer, and binding was detected using goat anti-hCRP antibody (1:3,000; 1 mg/ml; Bethyl: A80-125A) and donkey anti-goat (DAG) POD-conjugated antibody (DAG-POD; 1:5,000; 2,5mg/ml; Invitrogen, Waltham, Massachusetts, USA: A16005) for CRP (pCRP and mCRP) and goat anti-human-IgG-POD (1:3,000, 1 mg/ml; Rockland Immunochemical, Philadelphia, Pennsylvania, USA,, #109-035-003) for IgG1. ..



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Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

Journal: STAR Protocols

Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system

doi: 10.1016/j.xpro.2026.104471

Figure Lengend Snippet: Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

Article Snippet: Note: We use commercial flow cytometry staining buffer from eBioscience (Cat# 00-4222-26), which is PBS-based formulation designed to prevent non-specific antibody binding and maintain cell stability during flow cytometry.

Techniques: Flow Cytometry, Isolation, Staining