odyssey blocking buffer (Rockland Immunochemicals)

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Rockland Immunochemicals odyssey blocking buffer
Odyssey Blocking Buffer, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blocking+buffer/Blocking+Buffer+(10X)+for+THIMEROSAL+FREE/pmc05701066-242-4-7
Average 93 stars, based on 28 article reviews

odyssey blocking buffer - by Bioz Stars, 2026-10

93/100 stars

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Hybridization:

Article Title: Diverse spinal commissural neuron populations revealed by fate mapping and molecular profiling using a novel Robo3 Cre mouse
Article Snippet: .. For immunofluorescent detection of tdTomato following hybridization, sections were first blocked in 3% donkey serum and 0.1% Triton X-100 in PBS, and then incubated with a polyclonal rabbit antibody against Red Fluorescent Protein (RFP) (1:1000, Rockland Immunochemicals) in blocking solution O/N at 4°C. .. Sections were washed with 0.1% Triton X-100 in PBS, incubated with an Alexa488-conjugated donkey secondary antibody (1:500–1:1000, Invitrogen) for 1 h. The slides were then washed in 0.1% Triton X-100 in PBS, mounted under Fluoromount G (Electron Microscopy Sciences), and microscope images were pseudo-colored after acquisition.

Incubation:

Article Title: Functional Study of Haemophilus ducreyi Cytolethal Distending Toxin Subunit B
Article Snippet: Cells were then sonicated for 10 s and boiled at 90 °C for 5 min. Proteins were separated on 10% acrylamide SDS-PAGE and transferred to a nitrocellulose membrane (Amersham, Little Chalfont, UK). .. Membranes were blocked for 1 h with blocking buffer (Tris-HCl 20 mM pH 7, NaCl 150 mM (TBS 1×) 50/50 ( v/v ) with Odyssey Blocking Buffer (Rockland, Rockland, MA, USA), Tween 20 0.05%, sodium azide 0.02%) and then incubated with primary antibody diluted in blocking buffer. .. AKT was detected by 1/1000 rabbit anti-AKT antibody (Cell Signaling, Danvers, MA, USA), p-AKT by 1/1000 mouse anti-p-AKT S473 antibody (Cell Signaling) and GAPDH by 1/5000 rabbit anti-GAPDH antibody (GeneTex, Irvine, CA, USA).

Article Title: A Cre-dependent massively parallel reporter assay allows for cell-type specific assessment of the functional effects of genetic variants in vivo
Article Snippet: .. For immunostaining, sections were incubated in a blocking solution (1× PBS, 5% donkey serum, 0.25% Triton-X 100) for 1 h in a 12 well plate at room temperature, then with rabbit anti-RFP primary antibody (1:500; Rockland catalog #600-401-379) in blocking solution overnight in a sealed 12 well plate at 4°C. .. Following three five-minute washes in PBS, sections were incubated in donkey anti-rabbit Alexa Fluor 568 secondary antibody (1:1000, Invitrogen catalog #A10042) and DAPI (in blocking solution for 1 h. Sections were washed as before, and during the second wash, 1 μg/mL DAPI was added.

Article Title: Activation of the dorsal septum increases alcohol consumption in male C57BL/6J mice
Article Snippet: .. Tissue was incubated for 24-hr in the primary antibody solution that consisted of the blocking solution and mouse anti-RFP (1:500; Rockland Immunohistochemicals). .. The following day, tissue was washed in 1xPBS and incubated in a secondary solution that consisted of Alexa-Fluor 594 donkey anti-mouse (1:200; Jackson Immuno) in 0.1% Triton X-100 for 2-hr.

Article Title: Multicenter reverse-phase protein array data integration
Article Snippet: .. Slides were hydrated in deionized water (four 15-min washes), washed with TBS-T (three 5-min washes), and incubated with 50% (v/v) blocking buffer for fluorescent western blotting (Rockland Immunochemicals, Limerick, PA, USA, cat. no. MB-070) in TBS containing 5 mM sodium fluoride and 1 mM sodium orthovanadate (hereafter, Rockland blocking solution) for 2 h. Slides were washed with TBS-T (four 5-min washes) and incubated with primary antibodies (diluted in Rockland blocking solution; Online-only ) overnight at 4°C with agitation on a rocking platform. ..

Article Title: Activation of the dorsal septum increases alcohol consumption in male C57BL/6J mice
Article Snippet: Brains were extracted and post-fixed in PFA for 24 h. Tissue was sliced in 40 uM sections using a Vibratome and serial sections containing the entirety of the dSep were processed for immunohistochemistry to visualize the mCherry tag. .. Tissue was washed in 1xPBS, permeabilized in 0.5% Triton X-100 for 30 min, and blocked in 0.1% Triton X-100 and Normal Donkey Serum (NDS; 10%) for 1 h. Tissue was incubated for 24 h in the primary antibody solution that consisted of the blocking solution and mouse anti-RFP (1:500; Rockland Immunohistochemicals). .. The following day, tissue was washed in 1xPBS and incubated in a secondary solution that consisted of Alexa-Fluor 594 donkey anti-mouse (1:200; Jackson Immuno) in 0.1% Triton X-100 for 2 h. Tissue was washed in 1xPBS, mounted on Permafrost Plus slides, and coverslipped for Vectashield Hardset with DAPI.

Article Title: BNIP3L/NIX is required for elimination of mitochondria, endoplasmic reticulum and Golgi apparatus during eye lens organelle-free zone formation
Article Snippet: Proteins were electroblotted onto polyvinylidine difluoride membranes (Tran-Blot® Turbo TM Transfer Packs, Bio-Rad) using the Bio-Rad Tran-Blot® Turbo TM transfer system following the manufacturer’s instructions. .. Membranes were blocked in either 5% nonfat dried milk in TBS-T or 5% BSA in TBS-T or Rockland block (catalog no. MB-070) for 1 hour at room temperature, followed by incubation with a primary antibody diluted in appropriate blocking solution overnight at 4 °C. ..

Blocking Assay:

Immunostaining:

Western Blot:

other:

Article Title: Cell transfection of purified cytolethal distending toxin B subunits allows comparing their nuclease activity while plasmid degradation assay does not
Article Snippet: Immunoblots were revealed with an Odyssey Infrared Imaging Scanner (Li-Cor ScienceTec).

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