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rabbit serum  (Rockland Immunochemicals)


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    Structured Review

    Rockland Immunochemicals rabbit serum
    Rabbit Serum, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+buffer/Rabbit+Serum/pmc03091179-184-0-4
    Average 93 stars, based on 52 article reviews
    rabbit serum - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Affinity Purification:

    Article Title: A Tumor-Selective Monoclonal Antibody from Immunization with a Tumor-Associated Mucin Glycopeptide
    Article Snippet: .. Based on the remarkable specificity displayed in these preliminary results, a second batch of polyclonal serum was raised in rabbits in a collaboration with Rockland Immunochemicals (Limerick, PA) and part of this serum was affinity purified on a Sepharose column conjugated with the thiol-terminated molecule 1 as described in Materials and Methods . ..

    Article Title: Micromirs
    Article Snippet: The proteins were transferred to a nitrocellulose membrane using iBlot (Invitrogen) according to manufacturers instructions. .. The membrane was incubated overnight at 4° C. with the primary affinity purified rabbit serum antibody Pdcd4 (Rockland) with a 1:2000 concentration. .. As control, anti-beta tubulin antibodies (Thermo Scientific) were used at a 1:5000 dilution.

    Membrane:

    Article Title: Deciphering the Enigma of the Histone H2A.Z-1/H2A.Z-2 Isoforms: Novel Insights and Remaining Questions
    Article Snippet: SDS-PAGE separated proteins were transferred to a nitrocellulose membrane (GE Healthcare Life Sciences). .. The membrane was probed with different antibodies at indicated dilutions: H2A.Z 1:2000 (Ab 1474, Abcam, Toronto, ON, Canada), and H4 1:50,000 (rabbit serum produced in-house), followed by incubation with a secondary antibody conjugated to fluorescent dye, IRDye 800 Anti-rabbit IgG 1:10,000 (Rockland Antibodies & Assays, Gilbertsville, PA, USA). .. Immunoblots were scanned using Li-Cor Odyssey (LI-COR Biosciences).

    Article Title: Micromirs
    Article Snippet: The proteins were transferred to a nitrocellulose membrane using iBlot (Invitrogen) according to manufacturers instructions. .. The membrane was incubated overnight at 4° C. with the primary affinity purified rabbit serum antibody Pdcd4 (Rockland) with a 1:2000 concentration. .. As control, anti-beta tubulin antibodies (Thermo Scientific) were used at a 1:5000 dilution.

    Produced:

    Article Title: Deciphering the Enigma of the Histone H2A.Z-1/H2A.Z-2 Isoforms: Novel Insights and Remaining Questions
    Article Snippet: SDS-PAGE separated proteins were transferred to a nitrocellulose membrane (GE Healthcare Life Sciences). .. The membrane was probed with different antibodies at indicated dilutions: H2A.Z 1:2000 (Ab 1474, Abcam, Toronto, ON, Canada), and H4 1:50,000 (rabbit serum produced in-house), followed by incubation with a secondary antibody conjugated to fluorescent dye, IRDye 800 Anti-rabbit IgG 1:10,000 (Rockland Antibodies & Assays, Gilbertsville, PA, USA). .. Immunoblots were scanned using Li-Cor Odyssey (LI-COR Biosciences).

    Incubation:

    Article Title: Deciphering the Enigma of the Histone H2A.Z-1/H2A.Z-2 Isoforms: Novel Insights and Remaining Questions
    Article Snippet: SDS-PAGE separated proteins were transferred to a nitrocellulose membrane (GE Healthcare Life Sciences). .. The membrane was probed with different antibodies at indicated dilutions: H2A.Z 1:2000 (Ab 1474, Abcam, Toronto, ON, Canada), and H4 1:50,000 (rabbit serum produced in-house), followed by incubation with a secondary antibody conjugated to fluorescent dye, IRDye 800 Anti-rabbit IgG 1:10,000 (Rockland Antibodies & Assays, Gilbertsville, PA, USA). .. Immunoblots were scanned using Li-Cor Odyssey (LI-COR Biosciences).

    Article Title: SLAMF6 as a Regulator of Exhausted CD8+ T Cells in Cancer
    Article Snippet: .. Surface staining was done by first incubation with 20% rabbit serum (Rockland on July 23, 2019. .. © 2019 American Association for Cancer Research. cancerimmunolres.aacrjournals.org Downloaded from Immunochemicals) + Fc block (Biolegend) for 10 minutes at RT and the prepared antibody cocktails were incubated with cells for 30 minutes at 4oC.

    Article Title: Immunocytochemical assessment of cell differentiation of podoplanin-positive osteoblasts into osteocytes in murine bone.
    Article Snippet: In this study, we examined the immunolocalization of podoplanin/E11, CD44, actin filaments, and phosphorylated ezrin in the osteoblasts on the verge of differentiating into osteocytes in murine femora and tibiae.. When observing under stimulated emission depletion microscopy, unlike podoplanin-negative osteoblasts, podoplanin-positive osteoblasts showed a rearranged assembly of actin filaments along the cell membranes which resembled that of embedded osteocytes.. In the metaphysis, i.e., the bone remodeling site, CD44-bearing osteoclasts were either proximal to or in contact with podoplanin-positive osteoblasts, but the podoplanin-positive osteoblasts also localized CD44 on their own cell surface.

    Article Title: Immunostimulant effect of heat-inactivated Mycobacterium bovis in mice challenged with vector-borne pathogens.
    Article Snippet: Trained immunity is defined as an enhanced state of the innate system which leads to an improved immune response against related or non-related pathogens.. Bacillus Calmette-Guérin (BCG) vaccine, a live attenuated Mycobacterium bovis strain, is currently one of the main inductors of trained immunity.. The objective of the present study was to evaluate the protective effects of heat-inactivated M. bovis (HIMB) against Plasmodium berghei and Borrelia burgdorferi and characterize the immunological mechanisms involved.

    Article Title: Micromirs
    Article Snippet: The proteins were transferred to a nitrocellulose membrane using iBlot (Invitrogen) according to manufacturers instructions. .. The membrane was incubated overnight at 4° C. with the primary affinity purified rabbit serum antibody Pdcd4 (Rockland) with a 1:2000 concentration. .. As control, anti-beta tubulin antibodies (Thermo Scientific) were used at a 1:5000 dilution.

    Staining:

    Article Title: SLAMF6 as a Regulator of Exhausted CD8+ T Cells in Cancer
    Article Snippet: .. Surface staining was done by first incubation with 20% rabbit serum (Rockland on July 23, 2019. .. © 2019 American Association for Cancer Research. cancerimmunolres.aacrjournals.org Downloaded from Immunochemicals) + Fc block (Biolegend) for 10 minutes at RT and the prepared antibody cocktails were incubated with cells for 30 minutes at 4oC.

    Negative Control:

    Article Title: Immunocytochemical assessment of cell differentiation of podoplanin-positive osteoblasts into osteocytes in murine bone.
    Article Snippet: In this study, we examined the immunolocalization of podoplanin/E11, CD44, actin filaments, and phosphorylated ezrin in the osteoblasts on the verge of differentiating into osteocytes in murine femora and tibiae.. When observing under stimulated emission depletion microscopy, unlike podoplanin-negative osteoblasts, podoplanin-positive osteoblasts showed a rearranged assembly of actin filaments along the cell membranes which resembled that of embedded osteocytes.. In the metaphysis, i.e., the bone remodeling site, CD44-bearing osteoclasts were either proximal to or in contact with podoplanin-positive osteoblasts, but the podoplanin-positive osteoblasts also localized CD44 on their own cell surface.

    Cell Culture:

    Article Title: Immunostimulant effect of heat-inactivated Mycobacterium bovis in mice challenged with vector-borne pathogens.
    Article Snippet: Trained immunity is defined as an enhanced state of the innate system which leads to an improved immune response against related or non-related pathogens.. Bacillus Calmette-Guérin (BCG) vaccine, a live attenuated Mycobacterium bovis strain, is currently one of the main inductors of trained immunity.. The objective of the present study was to evaluate the protective effects of heat-inactivated M. bovis (HIMB) against Plasmodium berghei and Borrelia burgdorferi and characterize the immunological mechanisms involved.

    Concentration Assay:

    Article Title: Micromirs
    Article Snippet: The proteins were transferred to a nitrocellulose membrane using iBlot (Invitrogen) according to manufacturers instructions. .. The membrane was incubated overnight at 4° C. with the primary affinity purified rabbit serum antibody Pdcd4 (Rockland) with a 1:2000 concentration. .. As control, anti-beta tubulin antibodies (Thermo Scientific) were used at a 1:5000 dilution.



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