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protein free rapid blocking buffer  (Epizyme Inc)

 
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    Epizyme Inc protein free rapid blocking buffer
    Protein Free Rapid Blocking Buffer, supplied by Epizyme Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+buffer/free+protein/pm42309466-70-4-9
    Average 86 stars, based on 1 article reviews
    protein free rapid blocking buffer - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Membrane:

    Article Title: OV16 Improves Radiation-Induced Intestinal Injury by Targeting Transglutaminase 2.
    Article Snippet: Proteins were separated by SDS-PAGE (PG212, Epizyme, Shanghai, China) and transferred onto a PVDF membrane (IPVH00010, Merck Millipore, Darmstadt, Germany). .. The membrane was then blocked with a protein-free rapid blocking solution (PS108P, EpiZyme, China) for 0.5 h, and then incubated with the specific primary antibody overnight. ..

    Blocking Assay:

    Article Title: OV16 Improves Radiation-Induced Intestinal Injury by Targeting Transglutaminase 2.
    Article Snippet: Proteins were separated by SDS-PAGE (PG212, Epizyme, Shanghai, China) and transferred onto a PVDF membrane (IPVH00010, Merck Millipore, Darmstadt, Germany). .. The membrane was then blocked with a protein-free rapid blocking solution (PS108P, EpiZyme, China) for 0.5 h, and then incubated with the specific primary antibody overnight. ..

    Article Title: Ultrasound-programmable tumor extracellular vesicles delivery system for dual immune-epigenetic therapy against colorectal cancer metastasis.
    Article Snippet: Ultrasound-programmable tumor extracellular vesicles delivery system for dual immune–epigenetic therapy against colorectal cancer metastasis Congjian Wen, Bing Xiong, Yin Shu, Wenyun Guo, Sirui Wang, Lizhou Lin, Mingrui Zhu, Haochen Liu, Jieyi Ping, Jirong Xie, Hongjian Xu, Chunya Ji, Erxuan Wu, Boyang Zhou, Huixiong Xu, Haohao Yin PII: S0168-3659(26)00517-1 DOI: https://doi.org/10.1016/j.jconrel.2026.115114 Reference: COREL 115114 To appear in: Journal of Controlled Release

    Article Title: SIRT5 mediates the pro-osteogenic effects of estrogen through FDX1 demalonylation and cuproptosis inhibition in mesenchymal stem cells
    Article Snippet: Protein samples were initially separated via 10% or 12.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE; PG112; PG113, Epizyme Biotech) and then transferred onto polyvinylidene fluoride (PVDF) membrane (PR05509, Merck). .. Membranes were blocked with protein-free rapid blocking buffer (PS108P, Epizyme Biotech) before overnight incubation with primary antibodies. .. After washing with Tris-buffered saline-Tween 20 three times, membranes were incubated with BeyoWBTM HRP-labeled Goat Anti-Rabbit I (P0948, Beyotime) or BeyoWBTM HRP-labeled Goat Anti-Mouse (P0946, Beyotime) at room temperature for 1 h. Finally, blots were visualized using a chemiluminescent detection kit (160072, Saimike Biotech), and images were acquired using the Bio-Rad ChemiDoc XRS + system (Bio-Rad, Hercules, California, USA).

    Article Title: Sensory nerve-derived semaphorin 3A promotes bone healing through macrophage immunomodulation.
    Article Snippet: Sensory innervation has emerged as a crucial regulator of skeletal homeostasis and regeneration.. Growing evidence indicates that disruption of sensory nerves is associated with excessive inflammation and impaired tissue healing.. However, the underlying mechanisms by which neuronal signaling regulates the immune microenvironment to orchestrate bone repair remain unclear.

    Article Title: Integrated toxicological analysis links air pollution-associated acute respiratory distress syndrome signatures to benzo[a]pyrene-induced acute lung injury.
    Article Snippet: .. Subsequent to transfer, membranes were blocked for 60 min employing 1 × Rapid Protein-Free Blocking Buffer (PS108P; Epizyme Biomedical Technology Co., Ltd., Shanghai, China). .. Blocked membranes were then incubated overnight at 4◦C with primary antibodies targeting ETS2 (1:1000), VNN1 (1:1000), and GAPDH (1:5000), all sourced from Proteintech Group, Inc.

    Incubation:

    Article Title: OV16 Improves Radiation-Induced Intestinal Injury by Targeting Transglutaminase 2.
    Article Snippet: Proteins were separated by SDS-PAGE (PG212, Epizyme, Shanghai, China) and transferred onto a PVDF membrane (IPVH00010, Merck Millipore, Darmstadt, Germany). .. The membrane was then blocked with a protein-free rapid blocking solution (PS108P, EpiZyme, China) for 0.5 h, and then incubated with the specific primary antibody overnight. ..

    Article Title: Ultrasound-programmable tumor extracellular vesicles delivery system for dual immune-epigenetic therapy against colorectal cancer metastasis.
    Article Snippet: Ultrasound-programmable tumor extracellular vesicles delivery system for dual immune–epigenetic therapy against colorectal cancer metastasis Congjian Wen, Bing Xiong, Yin Shu, Wenyun Guo, Sirui Wang, Lizhou Lin, Mingrui Zhu, Haochen Liu, Jieyi Ping, Jirong Xie, Hongjian Xu, Chunya Ji, Erxuan Wu, Boyang Zhou, Huixiong Xu, Haohao Yin PII: S0168-3659(26)00517-1 DOI: https://doi.org/10.1016/j.jconrel.2026.115114 Reference: COREL 115114 To appear in: Journal of Controlled Release

    Article Title: Adaptive Spo11 RNA editing gate optimizes meiosis I pace and mitotic proliferation while preserving ascospore formation.
    Article Snippet: .. For loading control detection, membranes were stripped using stripping buffer (PS107S, Epizyme Biotech, China) for 10 min and washed with 1× TBST for 5 min before incubation overnight at 4°C with anti–glyceraldehyde- 3- phosphate dehydrogenase (GAPDH) antibody (Sangon Biotech, China). ..

    Article Title: SIRT5 mediates the pro-osteogenic effects of estrogen through FDX1 demalonylation and cuproptosis inhibition in mesenchymal stem cells
    Article Snippet: Protein samples were initially separated via 10% or 12.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE; PG112; PG113, Epizyme Biotech) and then transferred onto polyvinylidene fluoride (PVDF) membrane (PR05509, Merck). .. Membranes were blocked with protein-free rapid blocking buffer (PS108P, Epizyme Biotech) before overnight incubation with primary antibodies. .. After washing with Tris-buffered saline-Tween 20 three times, membranes were incubated with BeyoWBTM HRP-labeled Goat Anti-Rabbit I (P0948, Beyotime) or BeyoWBTM HRP-labeled Goat Anti-Mouse (P0946, Beyotime) at room temperature for 1 h. Finally, blots were visualized using a chemiluminescent detection kit (160072, Saimike Biotech), and images were acquired using the Bio-Rad ChemiDoc XRS + system (Bio-Rad, Hercules, California, USA).

    Article Title: Sensory nerve-derived semaphorin 3A promotes bone healing through macrophage immunomodulation.
    Article Snippet: Sensory innervation has emerged as a crucial regulator of skeletal homeostasis and regeneration.. Growing evidence indicates that disruption of sensory nerves is associated with excessive inflammation and impaired tissue healing.. However, the underlying mechanisms by which neuronal signaling regulates the immune microenvironment to orchestrate bone repair remain unclear.

    Control:

    Article Title: Adaptive Spo11 RNA editing gate optimizes meiosis I pace and mitotic proliferation while preserving ascospore formation.
    Article Snippet: .. For loading control detection, membranes were stripped using stripping buffer (PS107S, Epizyme Biotech, China) for 10 min and washed with 1× TBST for 5 min before incubation overnight at 4°C with anti–glyceraldehyde- 3- phosphate dehydrogenase (GAPDH) antibody (Sangon Biotech, China). ..

    Stripping Membranes:

    Article Title: Adaptive Spo11 RNA editing gate optimizes meiosis I pace and mitotic proliferation while preserving ascospore formation.
    Article Snippet: .. For loading control detection, membranes were stripped using stripping buffer (PS107S, Epizyme Biotech, China) for 10 min and washed with 1× TBST for 5 min before incubation overnight at 4°C with anti–glyceraldehyde- 3- phosphate dehydrogenase (GAPDH) antibody (Sangon Biotech, China). ..

    SDS Page:

    Article Title: A combinatorial therapeutic platform based on graphene-collagen cryogel and apoptotic vesicles for modulating hypertrophic scars.
    Article Snippet: Cells were lysed in RIPA buffer (Epizyme, PC101) with protease inhibitors (Thermo ScientificTM, 78430; 4°C, 30min). .. After denaturation (100°C, 10min) with loading buffer (Epizyme, LT101), samples (20 μg/lane) were loaded onto the SDS-PAGE gel (FuturePAGE TM , ET15420LGel; 4-20% 15 wells) and transferred to PVDF membranes (AmershamTM, GE10600023) using a wet transfer system. .. Membranes were blocked with 5% non-fat milk (Epizyme, PS112), probed with primary antibodies (4°C, overnight) and HRP-conjugated secondary antibodies (RT, 1h), then detected with ECL (Epizyme, Omni-ECLTM SQ201).

    Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
    Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.

    Cell Counting:

    Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
    Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.

    Staining:

    Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
    Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.

    Recombinant:

    Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
    Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.

    Activity Assay:

    Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
    Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.

    Calcium Colorimetric Assay:

    Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
    Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.



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    gels invitrogen nw04120box tbs blocking buffer li cor 927 60001 clarity western ecl substrate biorad 1705060 oligomycin sigma o4876 carbonyl cyanide  (Thermo Fisher)
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    Gels Invitrogen Nw04120box Tbs Blocking Buffer Li Cor 927 60001 Clarity Western Ecl Substrate Biorad 1705060 Oligomycin Sigma O4876 Carbonyl Cyanide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    flow cytometry staining buffer  (Thermo Fisher)
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    Thermo Fisher flow cytometry staining buffer
    Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
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    bullet blocking one buffer  (Nacalai)
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    Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
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    Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Journal: STAR Protocols

    Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system

    doi: 10.1016/j.xpro.2026.104471

    Figure Lengend Snippet: Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Article Snippet: Note: We use commercial flow cytometry staining buffer from eBioscience (Cat# 00-4222-26), which is PBS-based formulation designed to prevent non-specific antibody binding and maintain cell stability during flow cytometry.

    Techniques: Flow Cytometry, Isolation, Staining