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v v sheep serum  (Rockland Immunochemicals)


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    Structured Review

    Rockland Immunochemicals v v sheep serum
    V V Sheep Serum, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+buffer/Sheep+Serum/pm18835445-60-12-15
    Average 85 stars, based on 6 article reviews
    v v sheep serum - by Bioz Stars, 2026-09
    85/100 stars

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    Related Articles

    other:

    Article Title: Expression of 20-hydroxyecdysone-induced genes in the silkworm brain and their functional analysis in post-embryonic development.
    Article Snippet: After several washes at room temperature, the brains were treated with 5% (v/v) sheep serum (Rockland, Gilbertsville, PA, USA) at 4 C for 15–20 h, followed by 2 h incubation with alkaline phosphatase-conjugated anti-digoxigenin immunoglobulin G (IgG; Roche Diagnostics) at room temperature.

    Article Title: EcR expression in the prothoracicotropic hormone-producing neurosecretory cells of the Bombyx mori brain.
    Article Snippet: After sequential washing with 50% (v ⁄ v) formamide, 5 · NaCl ⁄ Cit and NaCl ⁄Pi ⁄ 0.05% Tween20 at room temperature, the brains were treated with 5% (v ⁄ v) sheep serum (Rockland, Gilbertsville, PA, USA) at 4 C for 15–20 h, followed by a 2 h incubation with a 1 : 500 diluted alkaline phosphatase conjugated anti-digoxigenin IgG (Roche Diagnostics) at room temperature.

    Blocking Assay:

    Article Title: Differential requirement for classic and novel PKC isoforms in respiratory burst and phagocytosis in RAW 264.7 cells.
    Article Snippet: .. The following incubations were performed with Ab diluted in 10% sheep serum (30 min, 21°C): 1) rabbit anti-BSA, 1:250; 2) FITC-conjugated goat anti-rabbit, (Rockland, Gilbertsville, PA), 1:200; 3) permeabilize 5 min in 0.01% Triton X-100; 4) block as above; 5) rabbit anti-BSA; 6) Texas-red conjugated goat anti-rabbit, (ICN Pharmaceuticals, Aurora, OH), 1:250; and 7) Hoechst nuclear stain (Molecular Probes). ..

    Article Title: LDB2 is a novel diagnostic and prognostic biomarker and inhibits bladder cancer metastasis by activating p38 MAPK/ERK1/2/JNK signaling pathway
    Article Snippet: .. Antigen retrieval was carried out in citrate buffer at 110 °C for 10 min. Staining was carried out with the PV-6000 universal kit (ZSGB-BIO) as follows: endogenous peroxidase block, serum blocking with sheep serum (ZU-9022), incubation with anti-LDB2 primary antibody (Rockland, 1:500, 4 °C, overnight, protected from light), biotin-labeled secondary antibody (Thermo Fisher, 20 min, room temperature), and DAB chromogen development. ..

    Staining:

    Article Title: Differential requirement for classic and novel PKC isoforms in respiratory burst and phagocytosis in RAW 264.7 cells.
    Article Snippet: .. The following incubations were performed with Ab diluted in 10% sheep serum (30 min, 21°C): 1) rabbit anti-BSA, 1:250; 2) FITC-conjugated goat anti-rabbit, (Rockland, Gilbertsville, PA), 1:200; 3) permeabilize 5 min in 0.01% Triton X-100; 4) block as above; 5) rabbit anti-BSA; 6) Texas-red conjugated goat anti-rabbit, (ICN Pharmaceuticals, Aurora, OH), 1:250; and 7) Hoechst nuclear stain (Molecular Probes). ..

    Article Title: LDB2 is a novel diagnostic and prognostic biomarker and inhibits bladder cancer metastasis by activating p38 MAPK/ERK1/2/JNK signaling pathway
    Article Snippet: .. Antigen retrieval was carried out in citrate buffer at 110 °C for 10 min. Staining was carried out with the PV-6000 universal kit (ZSGB-BIO) as follows: endogenous peroxidase block, serum blocking with sheep serum (ZU-9022), incubation with anti-LDB2 primary antibody (Rockland, 1:500, 4 °C, overnight, protected from light), biotin-labeled secondary antibody (Thermo Fisher, 20 min, room temperature), and DAB chromogen development. ..

    Article Title: Enhanced generation of endothelial cells from CD34+ cells of the bone marrow in rheumatoid arthritis: possible role in synovial neovascularization.
    Article Snippet: .. Cultured CD34 cells were stained with saturating concentrations of antibodies, including fluorescein isothiocyanate (FITC)– conjugated anti–HLA–DR monoclonal antibody (mAb) (mouse IgG2b; Immunotech, Marseilles, France), FITCconjugated sheep anti-vWF IgG (Cosmo Bio, Tokyo, Japan), phycoerythrin (PE)–conjugated anti-CD14 mAb (mouse IgG2a; Immunotech), PE-conjugated anti-CD31 mAb (mouse IgG1; Immunotech), PE-conjugated murine IgG1 and IgG2a control mAb or FITC-conjugated murine IgG2b control mAb, which were raised against Aspergillus niger glucose oxide, an enzyme that is neither present nor inducible in mammalian tissues (Dako, Glostrup, Denmark), or FITC-conjugated control sheep IgG purified from normal sheep serum (Rockland, Gilbertsville, PA). ..

    Incubation:

    Article Title: LDB2 is a novel diagnostic and prognostic biomarker and inhibits bladder cancer metastasis by activating p38 MAPK/ERK1/2/JNK signaling pathway
    Article Snippet: .. Antigen retrieval was carried out in citrate buffer at 110 °C for 10 min. Staining was carried out with the PV-6000 universal kit (ZSGB-BIO) as follows: endogenous peroxidase block, serum blocking with sheep serum (ZU-9022), incubation with anti-LDB2 primary antibody (Rockland, 1:500, 4 °C, overnight, protected from light), biotin-labeled secondary antibody (Thermo Fisher, 20 min, room temperature), and DAB chromogen development. ..

    Cell Culture:

    Article Title: Enhanced generation of endothelial cells from CD34+ cells of the bone marrow in rheumatoid arthritis: possible role in synovial neovascularization.
    Article Snippet: .. Cultured CD34 cells were stained with saturating concentrations of antibodies, including fluorescein isothiocyanate (FITC)– conjugated anti–HLA–DR monoclonal antibody (mAb) (mouse IgG2b; Immunotech, Marseilles, France), FITCconjugated sheep anti-vWF IgG (Cosmo Bio, Tokyo, Japan), phycoerythrin (PE)–conjugated anti-CD14 mAb (mouse IgG2a; Immunotech), PE-conjugated anti-CD31 mAb (mouse IgG1; Immunotech), PE-conjugated murine IgG1 and IgG2a control mAb or FITC-conjugated murine IgG2b control mAb, which were raised against Aspergillus niger glucose oxide, an enzyme that is neither present nor inducible in mammalian tissues (Dako, Glostrup, Denmark), or FITC-conjugated control sheep IgG purified from normal sheep serum (Rockland, Gilbertsville, PA). ..

    Control:

    Article Title: Enhanced generation of endothelial cells from CD34+ cells of the bone marrow in rheumatoid arthritis: possible role in synovial neovascularization.
    Article Snippet: .. Cultured CD34 cells were stained with saturating concentrations of antibodies, including fluorescein isothiocyanate (FITC)– conjugated anti–HLA–DR monoclonal antibody (mAb) (mouse IgG2b; Immunotech, Marseilles, France), FITCconjugated sheep anti-vWF IgG (Cosmo Bio, Tokyo, Japan), phycoerythrin (PE)–conjugated anti-CD14 mAb (mouse IgG2a; Immunotech), PE-conjugated anti-CD31 mAb (mouse IgG1; Immunotech), PE-conjugated murine IgG1 and IgG2a control mAb or FITC-conjugated murine IgG2b control mAb, which were raised against Aspergillus niger glucose oxide, an enzyme that is neither present nor inducible in mammalian tissues (Dako, Glostrup, Denmark), or FITC-conjugated control sheep IgG purified from normal sheep serum (Rockland, Gilbertsville, PA). ..

    Purification:

    Article Title: Enhanced generation of endothelial cells from CD34+ cells of the bone marrow in rheumatoid arthritis: possible role in synovial neovascularization.
    Article Snippet: .. Cultured CD34 cells were stained with saturating concentrations of antibodies, including fluorescein isothiocyanate (FITC)– conjugated anti–HLA–DR monoclonal antibody (mAb) (mouse IgG2b; Immunotech, Marseilles, France), FITCconjugated sheep anti-vWF IgG (Cosmo Bio, Tokyo, Japan), phycoerythrin (PE)–conjugated anti-CD14 mAb (mouse IgG2a; Immunotech), PE-conjugated anti-CD31 mAb (mouse IgG1; Immunotech), PE-conjugated murine IgG1 and IgG2a control mAb or FITC-conjugated murine IgG2b control mAb, which were raised against Aspergillus niger glucose oxide, an enzyme that is neither present nor inducible in mammalian tissues (Dako, Glostrup, Denmark), or FITC-conjugated control sheep IgG purified from normal sheep serum (Rockland, Gilbertsville, PA). ..



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    Image Search Results


    Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Journal: STAR Protocols

    Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system

    doi: 10.1016/j.xpro.2026.104471

    Figure Lengend Snippet: Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Article Snippet: Note: We use commercial flow cytometry staining buffer from eBioscience (Cat# 00-4222-26), which is PBS-based formulation designed to prevent non-specific antibody binding and maintain cell stability during flow cytometry.

    Techniques: Flow Cytometry, Isolation, Staining