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ATCC random bred 3t3
Figure 1. Expression of the Aq molecule on <t>NIH/3T3</t> cells (A), thymic ﬁbroblasts (B) and LS48 cells (C). After adhesion for 24 h, the cells were stained with biotin labeled anti-mouse Aq (clone KH116) and FITC Streptavidin for 15 min at 37 ◦C in dark. Photomicrographs show light/ﬂuorescence from the respective area. Bar 100 µm.

Figure 1. Expression of the Aq molecule on <t>NIH/3T3</t> cells (A), thymic ﬁbroblasts (B) and LS48 cells (C). After adhesion for 24 h, the cells were stained with biotin labeled anti-mouse Aq (clone KH116) and FITC Streptavidin for 15 min at 37 ◦C in dark. Photomicrographs show light/ﬂuorescence from the respective area. Bar 100 µm.

Random Bred 3t3, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 783 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/block+randomisation+method/3T3-Swiss+albino/pm37445989-335-32-34
Average 95 stars, based on 783 article reviews

random bred 3t3 - by Bioz Stars, 2026-11

95/100 stars

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1) Product Images from "NIH/3T3 Fibroblasts Selectively Activate T Cells Specific for Posttranslationally Modified Collagen Type II."

Article Title: NIH/3T3 Fibroblasts Selectively Activate T Cells Specific for Posttranslationally Modified Collagen Type II.

Journal: International journal of molecular sciences

doi: 10.3390/ijms241310811

Figure 1. Expression of the Aq molecule on NIH/3T3 cells (A), thymic ﬁbroblasts (B) and LS48 cells (C). After adhesion for 24 h, the cells were stained with biotin labeled anti-mouse Aq (clone KH116) and FITC Streptavidin for 15 min at 37 ◦C in dark. Photomicrographs show light/ﬂuorescence from the respective area. Bar 100 µm.

Figure Legend Snippet: Figure 1. Expression of the Aq molecule on NIH/3T3 cells (A), thymic ﬁbroblasts (B) and LS48 cells (C). After adhesion for 24 h, the cells were stained with biotin labeled anti-mouse Aq (clone KH116) and FITC Streptavidin for 15 min at 37 ◦C in dark. Photomicrographs show light/ﬂuorescence from the respective area. Bar 100 µm.

Techniques Used: Expressing, Staining, Labeling

Figure 2. Antigen-presenting capacity of different types of ﬁbroblasts. The ability of LS48, NIH/3T3, synovial ﬁbroblasts (SF), thymic ﬁbroblasts (ThyF), and dermal ﬁbroblasts (DF) to process and present COL2 was evaluated by measuring IL-2 levels in culture medium following 24 h co-culture of ﬁbroblasts with COL2-speciﬁc HCQ.3 T-cell hybridoma and 50 µg/mL COL2. The mean of triplicate measurements for each sample is shown.

Figure Legend Snippet: Figure 2. Antigen-presenting capacity of different types of ﬁbroblasts. The ability of LS48, NIH/3T3, synovial ﬁbroblasts (SF), thymic ﬁbroblasts (ThyF), and dermal ﬁbroblasts (DF) to process and present COL2 was evaluated by measuring IL-2 levels in culture medium following 24 h co-culture of ﬁbroblasts with COL2-speciﬁc HCQ.3 T-cell hybridoma and 50 µg/mL COL2. The mean of triplicate measurements for each sample is shown.

Techniques Used: Co-Culture Assay

Figure 3. NIH/3T3 fibroblasts activate HCQ.3 cells even in the absence of COL2 antigen. (A) NIH/3T3 and HCQ.3 cells were co-cultured for 24 h with or without COL2 and glycosylated COL2259–273 peptide, as well as with or without addition of professional APCs. Murine thymic fibroblasts (B), LS48 fibroblasts (C), QB dermal fibroblasts (D), or QB synovial fibroblasts (E), and HCQ.3 cells were co-cultured for 24 h with or without COL2 and glycosylated COL2259–273 peptide, as well as with or without addition of professional APCs. All samples were assessed in triplicates. Data represent ± SEM.

Figure Legend Snippet: Figure 3. NIH/3T3 fibroblasts activate HCQ.3 cells even in the absence of COL2 antigen. (A) NIH/3T3 and HCQ.3 cells were co-cultured for 24 h with or without COL2 and glycosylated COL2259–273 peptide, as well as with or without addition of professional APCs. Murine thymic fibroblasts (B), LS48 fibroblasts (C), QB dermal fibroblasts (D), or QB synovial fibroblasts (E), and HCQ.3 cells were co-cultured for 24 h with or without COL2 and glycosylated COL2259–273 peptide, as well as with or without addition of professional APCs. All samples were assessed in triplicates. Data represent ± SEM.

Techniques Used: Cell Culture

Figure 4. Ability of NIH/3T3 ﬁbroblasts to activate different COL2-speciﬁc T-cell hybridomas. (A) NIH/3T3 ﬁbroblasts were co-cultured with different COL2-speciﬁc hybridomas that recognize either the non-modiﬁed COL2259–273 epitope or the posttranslationally modiﬁed (glycosylated and hydroxylated) immunodominant epitope. Upon 24 h of incubation, IL-2 levels in culture supernatant were measured by enzyme-linked immunosorbent assay (ELISA). (B) HCQ.3 hybridoma was co- cultured with different types of ﬁbroblasts—NIH/3T3, LS48, QB DF, QB SF, and ThyF. After 24 h, hybridoma activation was evaluated by measuring IL-2 content in co-culture supernatant. (C) Co- culture of BW 5147 cells with NIH/3T3 or LS48, QB DF, QB SF, and ThyF. Following 24 h of incubation, IL-2 levels in culture supernatant were measured by ELISA. All samples were analyzed in triplicate. Data represent ± SEM.

Figure Legend Snippet: Figure 4. Ability of NIH/3T3 ﬁbroblasts to activate different COL2-speciﬁc T-cell hybridomas. (A) NIH/3T3 ﬁbroblasts were co-cultured with different COL2-speciﬁc hybridomas that recognize either the non-modiﬁed COL2259–273 epitope or the posttranslationally modiﬁed (glycosylated and hydroxylated) immunodominant epitope. Upon 24 h of incubation, IL-2 levels in culture supernatant were measured by enzyme-linked immunosorbent assay (ELISA). (B) HCQ.3 hybridoma was co- cultured with different types of ﬁbroblasts—NIH/3T3, LS48, QB DF, QB SF, and ThyF. After 24 h, hybridoma activation was evaluated by measuring IL-2 content in co-culture supernatant. (C) Co- culture of BW 5147 cells with NIH/3T3 or LS48, QB DF, QB SF, and ThyF. Following 24 h of incubation, IL-2 levels in culture supernatant were measured by ELISA. All samples were analyzed in triplicate. Data represent ± SEM.

Techniques Used: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Activation Assay, Co-Culture Assay

Figure 5. NIH/3T3 ﬁbroblasts interaction with COL2-speciﬁc T-cell hybridomas is cell-cell contact- dependent. NIH/3T3 ﬁbroblasts and COL2-speciﬁc T-cell hybridomas were co-cultured for 24 h with or without transwell membrane. After 24 h of incubation, IL-2 levels in culture supernatant were measured by ELISA. Data represent ± SEM. All samples were assayed in triplicate.

Figure Legend Snippet: Figure 5. NIH/3T3 ﬁbroblasts interaction with COL2-speciﬁc T-cell hybridomas is cell-cell contact- dependent. NIH/3T3 ﬁbroblasts and COL2-speciﬁc T-cell hybridomas were co-cultured for 24 h with or without transwell membrane. After 24 h of incubation, IL-2 levels in culture supernatant were measured by ELISA. Data represent ± SEM. All samples were assayed in triplicate.

Techniques Used: Cell Culture, Membrane, Incubation, Enzyme-linked Immunosorbent Assay

Figure 6. Expression levels of MHCII and CD40L on different types of murine ﬁbroblasts. NIH/3T3, LS48, and QB synovial ﬁbroblasts were cultured in standard complete DMEM medium or in medium with 100 U/mL IFN-γ for 72 h. After that, the cells were trypsinized, stained with ﬂuorochrome- labeled antibodies, and analyzed by ﬂow-cytometry. (A) Untreated and IFN-γ-pulsed NIH/3T3, LS48, and QB synovial ﬁbroblasts were analyzed for MHC class II molecules expression using monoclonal MHCII antibody (clone M5/114). (B) Expression levels of CD40L on different types of murine ﬁbroblasts following standard culture and INF-γ treatment. (C) CD40, CD40L, and MHCII on NIH/3T3 cells were blocked using speciﬁc monoclonal antibodies. The ﬁbroblasts were then washed and co-cultured with HCQ.3 T-cell hybridomas. After 24 h of incubation, the IL-2 levels in the culture medium were analyzed by ELISA.

Figure Legend Snippet: Figure 6. Expression levels of MHCII and CD40L on different types of murine ﬁbroblasts. NIH/3T3, LS48, and QB synovial ﬁbroblasts were cultured in standard complete DMEM medium or in medium with 100 U/mL IFN-γ for 72 h. After that, the cells were trypsinized, stained with ﬂuorochrome- labeled antibodies, and analyzed by ﬂow-cytometry. (A) Untreated and IFN-γ-pulsed NIH/3T3, LS48, and QB synovial ﬁbroblasts were analyzed for MHC class II molecules expression using monoclonal MHCII antibody (clone M5/114). (B) Expression levels of CD40L on different types of murine ﬁbroblasts following standard culture and INF-γ treatment. (C) CD40, CD40L, and MHCII on NIH/3T3 cells were blocked using speciﬁc monoclonal antibodies. The ﬁbroblasts were then washed and co-cultured with HCQ.3 T-cell hybridomas. After 24 h of incubation, the IL-2 levels in the culture medium were analyzed by ELISA.

Techniques Used: Expressing, Cell Culture, Staining, Labeling, Cytometry, Bioprocessing, Incubation, Enzyme-linked Immunosorbent Assay

Figure 7. Effect of NIH/3T3 cell surface molecules blocking HCQ.3 activation. (A) NIH/3T3 cells were incubated for 1 h with anti-mouse CD106 or CD34 antibodies. HCQ.3 cells were treated with anti-mouse CD33, CD4, or TCR Vβ8 antibodies for 1 h. Thereafter, the cells were washed and co-cultured with untreated HCQ.3 or NIH/3T3 cells. After 24 h of co-culture, T-cell hybridoma activation was analyzed based on IL-2 measurements in culture medium. (B) NIH/3T3 fibroblasts were treated with anti-mouse CD44, CD55, CD90.2, or CD138 antibodies for 1 h. After that, the cells were washed and co-cultured with HCQ.3 T-cell hybridomas for 24 h. IL-2 content was measured by ELISA following co-culture. All samples were plated and analyzed in triplicate. Data are shown as ± SEM. * p < 0.05, ** p < 0.01 vs. NIH/3T3 + HCQ.3.

Figure Legend Snippet: Figure 7. Effect of NIH/3T3 cell surface molecules blocking HCQ.3 activation. (A) NIH/3T3 cells were incubated for 1 h with anti-mouse CD106 or CD34 antibodies. HCQ.3 cells were treated with anti-mouse CD33, CD4, or TCR Vβ8 antibodies for 1 h. Thereafter, the cells were washed and co-cultured with untreated HCQ.3 or NIH/3T3 cells. After 24 h of co-culture, T-cell hybridoma activation was analyzed based on IL-2 measurements in culture medium. (B) NIH/3T3 fibroblasts were treated with anti-mouse CD44, CD55, CD90.2, or CD138 antibodies for 1 h. After that, the cells were washed and co-cultured with HCQ.3 T-cell hybridomas for 24 h. IL-2 content was measured by ELISA following co-culture. All samples were plated and analyzed in triplicate. Data are shown as ± SEM. * p < 0.05, ** p < 0.01 vs. NIH/3T3 + HCQ.3.

Techniques Used: Blocking Assay, Activation Assay, Incubation, Cell Culture, Co-Culture Assay, Enzyme-linked Immunosorbent Assay

Multiple Displacement Amplification:

Article Title: Taxane-induced conformational changes in the microtubule lattice activate GEF-H1-dependent RhoA signalling.
Article Snippet: .. HT1080 (a gift from J. Klumperman, UMC Utrecht), U2OS (ATCC; HTB-96), Swiss 3T3 (ATCC; CCL-92), MDA-MB-231 (mCh-Lifeact; described in ref. 84), 1205Lu (Lifeact-eGFP; described in refs. 85,86) and HEK293T cells (ATCC; CRL-3216) were cultured in Dulbecco’s modified Eagle medium (DMEM; Capricorn Scientific or Gibco) supplemented with 10% fetal bovine serum (FBS; Corning) and 100 U ml−1 penicillin and 100 μg ml−1 streptomycin (1% Pen Strep; Sigma). .. Cells were kept at 37 °C with 5% CO2 and routinely tested for mycoplasma using a commercial assay (Mycoaltert assay, LT07-518; Lonza).

Cell Culture:

Article Title: The apelinergic system accelerates wound healing in both type 1 and 2 diabetic mice
Article Snippet: .. Fibroblasts isolated from mouse embryos (3T3-Swiss albino, CCL-92, ATCC, Manassas, VA, USA) or primary human dermal fibroblast (PCS-201-012) were trypsinized and cultured in DMEM 5% FBS and 1% penicillinstreptomycin. ..

Article Title: Discovery of novel murine PML isoforms
Article Snippet: .. Pml +/+ or Pml −/− MEFs (generously provided by Dr. Piero Paolo Pandolfi [ , ]), mouse 3T3 fibroblasts (ATCC, CCL-92), mouse 3T6 fibroblasts (ATCC, CCL-96), and mouse mammary gland cells (NMuMG) (ATCC, CRL-1636) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Merck, Cat. No. D6429) supplemented with 10% fetal bovine serum (Merck, Cat. No. F7524) and an antibiotic-antimycotic solution (Merck, Cat. No. ECM0010) at 37°C in a humidified atmosphere containing 5% CO 2 . ..

Modification:

Article Title: Oleoyl-hyaluronate nanoparticles for enhanced stability and bioactivity of encapsulated coenzyme Q10.
Article Snippet: .. HaCaT human keratinocytes (CLS collection, Germany) and 3 T3 Swiss albino mouse embryonic fibroblasts (ATCC collection, USA) were maintained in Dulbecco's Modified Eagle's Medium (DMEM), supplemented with 10% fetal bovine serum (FBS), 0.29 mg⋅mL− 1 L-glutamine, 100 U⋅mL− 1 penicillin, and 100 μg⋅mL− 1 streptomycin (all reagents from Sigma-Aldrich, USA) at 37 ◦C in a humidified incubator with 5% CO2. ..

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