lentiviral vector (Addgene inc)
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Addgene inc
lentiviral vector
Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blastx+function/pLenti+CMVie-IRES-BlastR+(Plasmid+%23119863)/pmc08547738-216-20-25
Average 93 stars, based on 32 article reviews
Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blastx+function/pLenti+CMVie-IRES-BlastR+(Plasmid+%23119863)/pmc08547738-216-20-25
Average 93 stars, based on 32 article reviews
lentiviral vector - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "Heparan sulfate proteoglycans serve as alternative receptors for low affinity LCMV variants"
Article Title: Heparan sulfate proteoglycans serve as alternative receptors for low affinity LCMV variants
Journal: PLoS Pathogens
doi: 10.1371/journal.ppat.1009996
Figure Legend Snippet: (A) Flow cytometry analysis of Dystroglycan-1 (IIH6; red) and Heparan sulfate (10E4; blue) expression in 293T WT, Δ DAG1 , Δ EXTL3 , and Δ DAG1 EXTL3 cells generated by CRISPR Cas9-mediated knockout. (B) Infection assay using a lentiviral (LV) reporter system encoding eGFP. LVs were pseudotyped with VSV-G, as a control, and low (HPI WT Y155H or Arm 53b L260F) or high (HPI high H155Y or Arm Cl13 260L) affinity LCMV GP variants. Cells were transduced with an MOI of 1 (determined for 293T WT) and eGFP signal was measured 72 h later by flow cytometry. (C) Infection assay with low (WE HPI or Arm 53b) and high (Arm Cl13) affinity variants of LCMV in different knockout variants of 293T cells. The cells were infected with an MOI of 1 (determined by semi-functional quantitative flow cytometry assay for 293T WT cells) for 2 h at 37°C and 16 h p.i. quantified by flow cytometry via LCMV N-staining. Shown are the means ± SD of three replicates.
Techniques Used: Flow Cytometry, Expressing, Generated, CRISPR, Knock-Out, Infection, Transduction, Functional Assay, Staining
Related Articles
Expressing:Article Title: Lysosomal TMEM165 controls cellular ion homeostasis and survival by mediating lysosomal Ca 2+ import and H + efflux. Article Snippet: .. The TMEM165-mCherry expression construct was generated by inserting mCherry into Article Title: Lysosomal TMEM165 controls cellular ion homeostasis and survival by mediating lysosomal Ca 2+ import and H + efflux. Article Snippet: Then, the TMEM165 cDNA was amplified from pCDNA3.1NEGFPTMEM165 (Genscript) and inserted into pLentiCMVie-mCherry-BlastR via NheI and XhoI sites. .. The Lyso-GCaMP8s expression construct was generated by inserting Lyso into Article Title: Lysosomal TMEM165 controls cellular ion homeostasis and survival by mediating lysosomal Ca 2+ import and H + efflux Article Snippet: Then, the TMEM165 cDNA was amplified from pCDNA3.1NEGFP-TMEM165 (Genscript) and inserted into pLentiCMVie-mCherry-BlastR via NheI and XhoI sites. .. The Lyso-GCaMP8s expression construct was generated by inserting Lyso into Construct:Article Title: Lysosomal TMEM165 controls cellular ion homeostasis and survival by mediating lysosomal Ca 2+ import and H + efflux. Article Snippet: .. The TMEM165-mCherry expression construct was generated by inserting mCherry into Article Title: Lysosomal TMEM165 controls cellular ion homeostasis and survival by mediating lysosomal Ca 2+ import and H + efflux. Article Snippet: Then, the TMEM165 cDNA was amplified from pCDNA3.1NEGFPTMEM165 (Genscript) and inserted into pLentiCMVie-mCherry-BlastR via NheI and XhoI sites. .. The Lyso-GCaMP8s expression construct was generated by inserting Lyso into Article Title: Lysosomal TMEM165 controls cellular ion homeostasis and survival by mediating lysosomal Ca 2+ import and H + efflux Article Snippet: Then, the TMEM165 cDNA was amplified from pCDNA3.1NEGFP-TMEM165 (Genscript) and inserted into pLentiCMVie-mCherry-BlastR via NheI and XhoI sites. .. The Lyso-GCaMP8s expression construct was generated by inserting Lyso into Generated:Article Title: Lysosomal TMEM165 controls cellular ion homeostasis and survival by mediating lysosomal Ca 2+ import and H + efflux. Article Snippet: .. The TMEM165-mCherry expression construct was generated by inserting mCherry into Article Title: Lysosomal TMEM165 controls cellular ion homeostasis and survival by mediating lysosomal Ca 2+ import and H + efflux. Article Snippet: Then, the TMEM165 cDNA was amplified from pCDNA3.1NEGFPTMEM165 (Genscript) and inserted into pLentiCMVie-mCherry-BlastR via NheI and XhoI sites. .. The Lyso-GCaMP8s expression construct was generated by inserting Lyso into Article Title: Lysosomal TMEM165 controls cellular ion homeostasis and survival by mediating lysosomal Ca 2+ import and H + efflux Article Snippet: Then, the TMEM165 cDNA was amplified from pCDNA3.1NEGFP-TMEM165 (Genscript) and inserted into pLentiCMVie-mCherry-BlastR via NheI and XhoI sites. .. The Lyso-GCaMP8s expression construct was generated by inserting Lyso into other:Article Title: Lysosomal TMEM165 controls cellular ion homeostasis and survival by mediating lysosomal Ca 2+ import and H + efflux. Article Snippet: Then, the TMEM165 cDNA was amplified from pCDNA3.1NEGFPTMEM165 (Genscript) and inserted into pLentiCMVie-mCherry-BlastR via NheI and XhoI sites. Article Title: Lysosomal TMEM165 controls cellular ion homeostasis and survival by mediating lysosomal Ca 2+ import and H + efflux. Article Snippet: Then, the GCaMP8s was amplified from Plasmid Preparation:Article Title: Active Merlin Binds RalB to Regulate Exocytosis Article Snippet: Human b-catenin GFP was a gift from Alpha Yap (Addgene plasmid # 71367). pEGFP-Akt1(WT) and pEGFP-Akt2 were a gift from Thomas Leonard & Ivan Yudushkin (Addgene plasmids # 86637, #86593. pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin was a gift from David Andrews (Addgene plasmid # 134860). pLenti-PGK-RAP1A-WT-neo was a gift from Roland Friedel (Addgene plasmid # 156173). pCMV-Vamp2-pHluorin was a gift from Brad Zuchero (Addgene plasmid # 190151). .. The plasmids pEGFP-C3-Exo70, pEGFP-C3-Sec8, pEGFP-C3-Exo84 and pEGFP-C3-Sec5 were a gift from Channing Der. (Addgene plasmids # 53761, # 53758, #53762, #53756). pLenti CMVie-IRES-BlastR was a gift from Article Title: KSHV reprograms host RNA splicing via FAM50A to activate STAT3 and drive oncogenic cellular transformation. Article Snippet: .. The Article Title: KSHV reprograms host RNA splicing via FAM50A to activate STAT3 and drive oncogenic cellular transformation Article Snippet: .. The |