virus encoding cas9 (Addgene inc)
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Addgene inc
virus encoding cas9
Virus Encoding Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1665 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blastx+function/lentiCas9-Blast+(Plasmid+%2352962)/pmc07197326-857-22-25
Average 96 stars, based on 1665 article reviews
Virus Encoding Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1665 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blastx+function/lentiCas9-Blast+(Plasmid+%2352962)/pmc07197326-857-22-25
Average 96 stars, based on 1665 article reviews
virus encoding cas9 - by Bioz Stars,
2026-10
96/100 stars
Images
1) Product Images from "Vitamin B6 addiction in acute myeloid leukemia"
Article Title: Vitamin B6 addiction in acute myeloid leukemia
Journal: Cancer cell
doi: 10.1016/j.ccell.2019.12.002
Figure Legend Snippet: KEY RESOURCES TABLE
Techniques Used: Plasmid Preparation, Recombinant, Mutagenesis, RNA Extraction, Gel Extraction, Clone Assay, Purification, Flow Cytometry, Sequencing, CRISPR, Functional Assay, shRNA, Software
Related Articles
Recombinant:Article Title: SPARCS enables scalable recovery of complex image-based phenotypes for genetic screening. Article Snippet: Human female U2OS and HeLa cells were cultured in DMEM (Thermo Fisher Scientific #41965062) supplemented with 10 % fetal calf serum (FCS) (gibco #10270-106), penicillin/streptomycin (Thermo Fisher Scientific #15-140-122) and 1 mM sodium pyruvate (Thermo .. REAGENT or RESOURCE SOURCE IDENTIFIER GPR89B_crRNA_2 rUrCrArCrUrCrCrArArUrCrArCrArCrCrArAr CrCrGrUrUrUrUrArGrArGrCrUrArUrGrCrU Integrated DNA Technologies n/a Recombinant DNA Genome-wide human Brunello CRISPR library (LentiGUIDE-Puro) Doench et al.57 Addgene-73178 Genome-wide human Brunello CRISPR library (LentiCRISPRv2) Doench et al.57 Addgene-73179 pFUGW-mCherry-hsLC3 This study, Jahreiss et al.103 Addgene-40827 pFUGW-LckLip-mNeon This study, Chertkova et al.104 Addgene-98821 pLenti6-MGAT-SEP-mRuby3 This study, Liu et al.75 Addgene-200937 pLenti6-GALT-SEP-mRuby3 This study, Liu et al.75 Addgene-200938 pBlast_hsGPR89B_FLAG This study n/a pFUGW_hsSTING1_mNeon This study n/a Lenti-Cas9-Blast Sanjana et Genome Wide:Article Title: SPARCS enables scalable recovery of complex image-based phenotypes for genetic screening. Article Snippet: Human female U2OS and HeLa cells were cultured in DMEM (Thermo Fisher Scientific #41965062) supplemented with 10 % fetal calf serum (FCS) (gibco #10270-106), penicillin/streptomycin (Thermo Fisher Scientific #15-140-122) and 1 mM sodium pyruvate (Thermo .. REAGENT or RESOURCE SOURCE IDENTIFIER GPR89B_crRNA_2 rUrCrArCrUrCrCrArArUrCrArCrArCrCrArAr CrCrGrUrUrUrUrArGrArGrCrUrArUrGrCrU Integrated DNA Technologies n/a Recombinant DNA Genome-wide human Brunello CRISPR library (LentiGUIDE-Puro) Doench et al.57 Addgene-73178 Genome-wide human Brunello CRISPR library (LentiCRISPRv2) Doench et al.57 Addgene-73179 pFUGW-mCherry-hsLC3 This study, Jahreiss et al.103 Addgene-40827 pFUGW-LckLip-mNeon This study, Chertkova et al.104 Addgene-98821 pLenti6-MGAT-SEP-mRuby3 This study, Liu et al.75 Addgene-200937 pLenti6-GALT-SEP-mRuby3 This study, Liu et al.75 Addgene-200938 pBlast_hsGPR89B_FLAG This study n/a pFUGW_hsSTING1_mNeon This study n/a Lenti-Cas9-Blast Sanjana et CRISPR:Article Title: SPARCS enables scalable recovery of complex image-based phenotypes for genetic screening. Article Snippet: Human female U2OS and HeLa cells were cultured in DMEM (Thermo Fisher Scientific #41965062) supplemented with 10 % fetal calf serum (FCS) (gibco #10270-106), penicillin/streptomycin (Thermo Fisher Scientific #15-140-122) and 1 mM sodium pyruvate (Thermo .. REAGENT or RESOURCE SOURCE IDENTIFIER GPR89B_crRNA_2 rUrCrArCrUrCrCrArArUrCrArCrArCrCrArAr CrCrGrUrUrUrUrArGrArGrCrUrArUrGrCrU Integrated DNA Technologies n/a Recombinant DNA Genome-wide human Brunello CRISPR library (LentiGUIDE-Puro) Doench et al.57 Addgene-73178 Genome-wide human Brunello CRISPR library (LentiCRISPRv2) Doench et al.57 Addgene-73179 pFUGW-mCherry-hsLC3 This study, Jahreiss et al.103 Addgene-40827 pFUGW-LckLip-mNeon This study, Chertkova et al.104 Addgene-98821 pLenti6-MGAT-SEP-mRuby3 This study, Liu et al.75 Addgene-200937 pLenti6-GALT-SEP-mRuby3 This study, Liu et al.75 Addgene-200938 pBlast_hsGPR89B_FLAG This study n/a pFUGW_hsSTING1_mNeon This study n/a Lenti-Cas9-Blast Sanjana et Article Title: FDXR upregulation by p63/p73 is a prognostic and therapeutic marker of adult T-cell leukemia/lymphoma. Article Snippet: .. Gene expression knockout with CRISPR/Cas9 HTLV-1 infected (MT-4) and ATL (ATL-55T) cell lines were transduced to stably express Cas9 using the Data-independent acquisition:Article Title: SPARCS enables scalable recovery of complex image-based phenotypes for genetic screening. Article Snippet: Human female U2OS and HeLa cells were cultured in DMEM (Thermo Fisher Scientific #41965062) supplemented with 10 % fetal calf serum (FCS) (gibco #10270-106), penicillin/streptomycin (Thermo Fisher Scientific #15-140-122) and 1 mM sodium pyruvate (Thermo .. REAGENT or RESOURCE SOURCE IDENTIFIER GPR89B_crRNA_2 rUrCrArCrUrCrCrArArUrCrArCrArCrCrArAr CrCrGrUrUrUrUrArGrArGrCrUrArUrGrCrU Integrated DNA Technologies n/a Recombinant DNA Genome-wide human Brunello CRISPR library (LentiGUIDE-Puro) Doench et al.57 Addgene-73178 Genome-wide human Brunello CRISPR library (LentiCRISPRv2) Doench et al.57 Addgene-73179 pFUGW-mCherry-hsLC3 This study, Jahreiss et al.103 Addgene-40827 pFUGW-LckLip-mNeon This study, Chertkova et al.104 Addgene-98821 pLenti6-MGAT-SEP-mRuby3 This study, Liu et al.75 Addgene-200937 pLenti6-GALT-SEP-mRuby3 This study, Liu et al.75 Addgene-200938 pBlast_hsGPR89B_FLAG This study n/a pFUGW_hsSTING1_mNeon This study n/a Lenti-Cas9-Blast Sanjana et Laser Capture Microdissection:Article Title: SPARCS enables scalable recovery of complex image-based phenotypes for genetic screening. Article Snippet: Human female U2OS and HeLa cells were cultured in DMEM (Thermo Fisher Scientific #41965062) supplemented with 10 % fetal calf serum (FCS) (gibco #10270-106), penicillin/streptomycin (Thermo Fisher Scientific #15-140-122) and 1 mM sodium pyruvate (Thermo .. REAGENT or RESOURCE SOURCE IDENTIFIER GPR89B_crRNA_2 rUrCrArCrUrCrCrArArUrCrArCrArCrCrArAr CrCrGrUrUrUrUrArGrArGrCrUrArUrGrCrU Integrated DNA Technologies n/a Recombinant DNA Genome-wide human Brunello CRISPR library (LentiGUIDE-Puro) Doench et al.57 Addgene-73178 Genome-wide human Brunello CRISPR library (LentiCRISPRv2) Doench et al.57 Addgene-73179 pFUGW-mCherry-hsLC3 This study, Jahreiss et al.103 Addgene-40827 pFUGW-LckLip-mNeon This study, Chertkova et al.104 Addgene-98821 pLenti6-MGAT-SEP-mRuby3 This study, Liu et al.75 Addgene-200937 pLenti6-GALT-SEP-mRuby3 This study, Liu et al.75 Addgene-200938 pBlast_hsGPR89B_FLAG This study n/a pFUGW_hsSTING1_mNeon This study n/a Lenti-Cas9-Blast Sanjana et Amplification:Article Title: Prime editing reveals the resistance landscape of EGFR tyrosine kinase domain variants Article Snippet: All PCR amplifications were performed using Q5 High-Fidelity DNA Polymerase according to the manufacturer’s protocol. .. Fragment 1 was amplified from Knock-Out:Article Title: SLC25A28 is a synthetic lethal vulnerability in nucleotide excision repair-deficient cancers Article Snippet: .. RT112/84 wild-type and ERCC4 -knockout isogenic cell lines were first transduced with Article Title: FDXR upregulation by p63/p73 is a prognostic and therapeutic marker of adult T-cell leukemia/lymphoma. Article Snippet: .. Gene expression knockout with CRISPR/Cas9 HTLV-1 infected (MT-4) and ATL (ATL-55T) cell lines were transduced to stably express Cas9 using the Transduction:Article Title: SLC25A28 is a synthetic lethal vulnerability in nucleotide excision repair-deficient cancers Article Snippet: .. RT112/84 wild-type and ERCC4 -knockout isogenic cell lines were first transduced with Stable Transfection:Article Title: SLC25A28 is a synthetic lethal vulnerability in nucleotide excision repair-deficient cancers Article Snippet: .. RT112/84 wild-type and ERCC4 -knockout isogenic cell lines were first transduced with Article Title: FDXR upregulation by p63/p73 is a prognostic and therapeutic marker of adult T-cell leukemia/lymphoma. Article Snippet: .. Gene expression knockout with CRISPR/Cas9 HTLV-1 infected (MT-4) and ATL (ATL-55T) cell lines were transduced to stably express Cas9 using the Expressing:Article Title: SLC25A28 is a synthetic lethal vulnerability in nucleotide excision repair-deficient cancers Article Snippet: .. RT112/84 wild-type and ERCC4 -knockout isogenic cell lines were first transduced with Gene Expression:Article Title: FDXR upregulation by p63/p73 is a prognostic and therapeutic marker of adult T-cell leukemia/lymphoma. Article Snippet: .. Gene expression knockout with CRISPR/Cas9 HTLV-1 infected (MT-4) and ATL (ATL-55T) cell lines were transduced to stably express Cas9 using the Infection:Article Title: FDXR upregulation by p63/p73 is a prognostic and therapeutic marker of adult T-cell leukemia/lymphoma. Article Snippet: .. Gene expression knockout with CRISPR/Cas9 HTLV-1 infected (MT-4) and ATL (ATL-55T) cell lines were transduced to stably express Cas9 using the Transfection:Article Title: SMCHD1 is a target for gene activation therapy to treat Prader-Willi syndrome Article Snippet: Guide RNA (gRNAs) were cloned into lentiGuide-Puro (Addgene #52963) and pFgH1UTPuro (Addgene #70183 with the GFP reporter replaced by puromycin resistance). .. HEK293T cells at 80% confluency were transfected via calcium phosphate-mediated transfection with the psPAX packaging vector, the VSVg envelope vector, and a plasmid containing either a SMCHD1 gRNA or Plasmid Preparation:Article Title: SMCHD1 is a target for gene activation therapy to treat Prader-Willi syndrome Article Snippet: Guide RNA (gRNAs) were cloned into lentiGuide-Puro (Addgene #52963) and pFgH1UTPuro (Addgene #70183 with the GFP reporter replaced by puromycin resistance). .. HEK293T cells at 80% confluency were transfected via calcium phosphate-mediated transfection with the psPAX packaging vector, the VSVg envelope vector, and a plasmid containing either a SMCHD1 gRNA or other:Article Title: Functional identification of non-coding regulatory regions that drive breast and skin carcinogenesis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Pooled sgRNA library – human nonFMRE control library (3,539 sgRNAs) GenScript (pooled chip synthesis) N/A Pooled NTC library (1,778 non-targeting control sgRNAs) Sanjana et al.81; Al-Zahrani et al.24 N/A Pooled dgRNA library – human FMRE CRISPR-A library GenScript (pooled chip synthesis) N/A Pooled sgRNA library – mouse mFMRE library (694 sgRNAs) GenScript (pooled chip synthesis) N/A Pooled sgRNA library – mouse nonFMRE control library (1,934 sgRNAs) GenScript (pooled chip synthesis) N/A Pooled NTC library for mouse (420 nontargeting control sgRNAs) This paper N/A Individual validation gRNAs (ZFP36L2, VMP1/miR21, TBC1D12, POLR3E, LNPEP, PTEN, TP53, AAVS1, TIGRE, UROD, B2M) This paper N/A RT-qPCR primers (PPIB, ZFP36L2, B2M, VMP1); sequences in Method Details This paper N/A Recombinant DNA pLKO-Cre lentiviral vector Loganathan et al.48; Loganathan et al.50 |