ncbi blast suite version 2 16 0 c hepaticus (Biotechnology Information)

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Biotechnology Information ncbi blast suite version 2 16 0 c hepaticus
$Expression and confirmation of the surface display of C. <t>hepaticus</t> mimotopes as a fusion with OmpC in E. coli DH5α Δ ompC and APEC PSUO78 Δ aroA Δ asd double mutant strains. ( A ) Coomassie brilliant blue–stained gel picture showing the outer membrane proteins extracted from the recombinant E. coli DH5α Δ ompC strains. The arrow indicates the position of OmpC (30 KDa). ( B ) Western blot image showing the recombinant His-tagged OmpC protein extracted from the E. coli DH5α Δ ompC strain. The arrow indicates the 48 kDa OmpC + mimotope fusion (lanes 4 and 5 in ( A )). ( C ) The western blot showing the recombinant outer membrane protein fractions extracted from APEC PSUO78 Δ aroA Δ asd carrying three different mimotopes. The arrow indicates the 48 kDa OmpC+ mimotope.$
Ncbi Blast Suite Version 2 16 0 C Hepaticus, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blast+algorithm/algorithm+blast/pmc13119680-123-16-14
Average 86 stars, based on 1 article reviews

ncbi blast suite version 2 16 0 c hepaticus - by Bioz Stars, 2026-10

86/100 stars

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1) Product Images from "Identification and Expression of Immunogenic Mimotopes of C. hepaticus Using an E. coli -Based Surface Display System"

Article Title: Identification and Expression of Immunogenic Mimotopes of C. hepaticus Using an E. coli -Based Surface Display System

Journal: Vaccines

doi: 10.3390/vaccines14040298

$Expression and confirmation of the surface display of C. hepaticus mimotopes as a fusion with OmpC in E. coli DH5α Δ ompC and APEC PSUO78 Δ aroA Δ asd double mutant strains. ( A ) Coomassie brilliant blue–stained gel picture showing the outer membrane proteins extracted from the recombinant E. coli DH5α Δ ompC strains. The arrow indicates the position of OmpC (30 KDa). ( B ) Western blot image showing the recombinant His-tagged OmpC protein extracted from the E. coli DH5α Δ ompC strain. The arrow indicates the 48 kDa OmpC + mimotope fusion (lanes 4 and 5 in ( A )). ( C ) The western blot showing the recombinant outer membrane protein fractions extracted from APEC PSUO78 Δ aroA Δ asd carrying three different mimotopes. The arrow indicates the 48 kDa OmpC+ mimotope.$
Figure Legend Snippet: Expression and confirmation of the surface display of C. hepaticus mimotopes as a fusion with OmpC in E. coli DH5α Δ ompC and APEC PSUO78 Δ aroA Δ asd double mutant strains. ( A ) Coomassie brilliant blue–stained gel picture showing the outer membrane proteins extracted from the recombinant E. coli DH5α Δ ompC strains. The arrow indicates the position of OmpC (30 KDa). ( B ) Western blot image showing the recombinant His-tagged OmpC protein extracted from the E. coli DH5α Δ ompC strain. The arrow indicates the 48 kDa OmpC + mimotope fusion (lanes 4 and 5 in ( A )). ( C ) The western blot showing the recombinant outer membrane protein fractions extracted from APEC PSUO78 Δ aroA Δ asd carrying three different mimotopes. The arrow indicates the 48 kDa OmpC+ mimotope.

Techniques Used: Expressing, Mutagenesis, Staining, Membrane, Recombinant, Western Blot

Comparison of C. hepaticus loads in liver and cecal samples across the experimental groups by quantitative real-time PCR. Group-4 (Mimotope-2, FliK), group-6 (mimotope-4, flagellin A), group-7 (mimotope-5, MOMP), and the control group (positive control: unvaccinated-challenged control group). ns: p > 0.05 (not significant), and **: p ≤ 0.01 (moderately/highly significant).

Figure Legend Snippet: Comparison of C. hepaticus loads in liver and cecal samples across the experimental groups by quantitative real-time PCR. Group-4 (Mimotope-2, FliK), group-6 (mimotope-4, flagellin A), group-7 (mimotope-5, MOMP), and the control group (positive control: unvaccinated-challenged control group). ns: p > 0.05 (not significant), and **: p ≤ 0.01 (moderately/highly significant).

Techniques Used: Comparison, Real-time Polymerase Chain Reaction, Control, Positive Control

other:

Article Title: Evaluation of two PCR protocols for the detection of Entamoeba histolytica/E. dispar/E. moshkovskii in stool samples.
Article Snippet: The diagnosis of amoebiasis (caused by Entamoeba histolytica) is performed using coprological techniques, but these have low sensitivity in cases of low parasite loads.. Furthermore, other species such as Entamoeba dispar and E. moshkovskii cannot be morphologically differentiated.. PCR could be an alternative.

Article Title: Integrating network pharmacology, transcriptomics, and experimental validation: Compound Baixianpi Formula targets IL-17A to inhibit dual PI3K-AKT/JAK2-STAT3 pathways for psoriasis improvement
Article Snippet: Primer sequences used are shown in Supplementary materials 2 and their specificity was confirmed using the BLAST algorithm of National Center for Biotechnology Information.

Article Title: Metatranscriptomic analysis of upper and lower respiratory tract microbiomes in patients with tuberculosis and community-acquired pneumonia.
Article Snippet: Microbial reads were compared to the National Center for Biotechnology Information nonredundant nucleic acid database for species annotation using Kraken2 (v2.1.2) [23].

Sequencing:

Article Title: G protein-coupled receptor 119 exerts anti-inflammatory effects on the liver of zebrafish (Danio rerio) via mediating G s /cAMP-dependent pathway.
Article Snippet: Hepatic inflammation is a prevalent health issue in farmed fish, which severely compromises liver function and immune homeostasis.. G protein–coupled receptor 119 (GPR119) has been implicated in metabolic and immune regulation in mammals; however, its role in teleost fish remains largely unexplored.. In this study, zebrafish (Danio rerio) GPR119 was cloned and systematically characterized with respect to its sequence features, tissue distribution, and subcellular localization.

Generated:

Article Title: Metagenomic surveillance of tick-borne pathogens and microbiomes in Huntingdon County, Pennsylvania
Article Snippet: .. The Kraken2 program [ ] then assigned National Center for Biotechnology Information (NCBI) taxonomic identifiers to the samples' sequences and generated taxonomy reports that were later used to create a table for species-level analysis. ..

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