amplification-free long-read sequencing (Oxford Nanopore)
90
Structured Review
Oxford Nanopore
amplification-free long-read sequencing
Amplification Free Long Read Sequencing, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bisulfite-based+microarray+methods/amplification+free+long+read+sequencing+library+preparation/pmc11742067-14-20-25
Average 90 stars, based on 1 article reviews
Amplification Free Long Read Sequencing, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bisulfite-based+microarray+methods/amplification+free+long+read+sequencing+library+preparation/pmc11742067-14-20-25
Average 90 stars, based on 1 article reviews
amplification-free long-read sequencing - by Bioz Stars,
2026-10
90/100 stars
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other:Article Title: Metagenomic sequencing detects human respiratory and enteric viruses in air samples collected from congregate settings. Article Snippet: Viral RNA was extracted from air samples, and complementary DNA (cDNA) was prepared using sequenceindependent single primer amplification (SISPA) for Article Title: Detection and Characterization of a Novel Picornavirus in European Badger ( Meles meles ) Article Snippet: The full-length genome of this novel strain was obtained by a sequence-independent single-primer amplification procedure in combination with Article Title: Exploring the Enteric Virome of Cats with Acute Gastroenteritis. Article Snippet: In addition, to further characterize the viral community associated with feline enteric disease, eight diarrhoeic specimens were assessed by combining a sequence-independent single-primer amplification (SISPA) approach with Article Title: Detection, Characterization and Sequencing of BTV Serotypes Circulating in Cuba in 2022 Article Snippet: Finally, the full genome sequences of 17 Cuban BTV isolates were recovered using a Sequence Independent Single Primer Amplification (SISPA) approach combined to Article Title: Precise cut-and-paste DNA insertion using engineered type V-K CRISPR-associated transposases. Article Snippet: CRISPR-associated transposases (CASTs) enable recombinationindependent, multi-kilobase DNA insertions at RNA-programmed genomic locations.. However, the utility of type V-K CASTs is hindered by high off-target integration and a transposition mechanism that results in a mixture of desired simple cargo insertions and undesired plasmid cointegrate products.. Here we overcome both limitations by engineering new CASTs with improved integration product purity and genome-wide specificity. Article Title: MethylBERT enables read-level DNA methylation pattern identification and tumour deconvolution using a Transformer-based model Article Snippet: Article Title: Metagenomic sequencing detects human respiratory and enteric viruses in air samples collected from congregate settings Article Snippet: Viral RNA was extracted from air samples, and complementary DNA (cDNA) was prepared using sequence-independent single primer amplification (SISPA) for Sequencing:Article Title: Enrichment of SARS-CoV-2 sequence from nasopharyngeal swabs whilst identifying the nasal microbiome. Article Snippet: Simultaneously characterising the genomic information of coronaviruses and the underlying nasal microbiome from a single clinical sample would help characterise infection and disease.. Metatranscriptomic approaches can be used to sequence SARS-CoV-2 (and other coronaviruses) and identify mRNAs associated with active transcription in the nasal microbiome.. However, given the large sequence background, unenriched metatranscriptomic approaches often do not sequence SARS-CoV-2 to sufficient read and coverage depth to obtain a consensus genome, especially with moderate and low viral loads from clinical samples. Amplification:Article Title: Enrichment of SARS-CoV-2 sequence from nasopharyngeal swabs whilst identifying the nasal microbiome. Article Snippet: Simultaneously characterising the genomic information of coronaviruses and the underlying nasal microbiome from a single clinical sample would help characterise infection and disease.. Metatranscriptomic approaches can be used to sequence SARS-CoV-2 (and other coronaviruses) and identify mRNAs associated with active transcription in the nasal microbiome.. However, given the large sequence background, unenriched metatranscriptomic approaches often do not sequence SARS-CoV-2 to sufficient read and coverage depth to obtain a consensus genome, especially with moderate and low viral loads from clinical samples. |