Review



infinium humanmethylation27 assay  (INFINIUM Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    INFINIUM Inc infinium humanmethylation27 assay
    Genomic coverage was quantified by the number of DNA methylation measurements that overlap with CpG islands (top row), gene promoters (center row) and a 1-kilobase tiling of the genome (bottom row). For MeDIP and MethylCap, the number of measurements is equal to the number of unique sequencing reads that fall inside each region. For RRBS, it refers to the number of valid DNA methylation measurements at CpGs within each region (one RRBS sequencing read typically yields one measurement, but can also give rise to more than one measurement if it contains several CpGs). For Infinium, the number of measurements is equal to the number of CpGs within each region that are present on the <t>HumanMethylation27</t> microarray. CpG islands were calculated using CgiHunter ( http://cgihunter.bioinf.mpi-inf.mpg.de/ ), requiring a minimum CpG observed vs. expected ratio of 0.6, a minimum GC content of 0.5 and a minimum length of 700 basepairs . Promoter regions were calculated based on Ensembl gene annotations, such that the region starts one kilo-base upstream of the annotated transcription start site (TSS) and extends to one kilobase downstream of the TSS. The genomic tiling was obtained by sliding a 1-kilobase window through the genome such that each tile starts at the position where the previous tile ends. No repeat-masking was performed for any of the three types of genomic regions. Data are shown for the HUES6 human ES cell line.
    Infinium Humanmethylation27 Assay, supplied by INFINIUM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bisulfite-based+microarray+methods/pmc03066564-27-73-72?v=INFINIUM+Inc
    Average 90 stars, based on 1 article reviews
    infinium humanmethylation27 assay - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Genome-wide mapping of DNA methylation: a quantitative technology comparison"

    Article Title: Genome-wide mapping of DNA methylation: a quantitative technology comparison

    Journal: Nature biotechnology

    doi: 10.1038/nbt.1681

    Genomic coverage was quantified by the number of DNA methylation measurements that overlap with CpG islands (top row), gene promoters (center row) and a 1-kilobase tiling of the genome (bottom row). For MeDIP and MethylCap, the number of measurements is equal to the number of unique sequencing reads that fall inside each region. For RRBS, it refers to the number of valid DNA methylation measurements at CpGs within each region (one RRBS sequencing read typically yields one measurement, but can also give rise to more than one measurement if it contains several CpGs). For Infinium, the number of measurements is equal to the number of CpGs within each region that are present on the HumanMethylation27 microarray. CpG islands were calculated using CgiHunter ( http://cgihunter.bioinf.mpi-inf.mpg.de/ ), requiring a minimum CpG observed vs. expected ratio of 0.6, a minimum GC content of 0.5 and a minimum length of 700 basepairs . Promoter regions were calculated based on Ensembl gene annotations, such that the region starts one kilo-base upstream of the annotated transcription start site (TSS) and extends to one kilobase downstream of the TSS. The genomic tiling was obtained by sliding a 1-kilobase window through the genome such that each tile starts at the position where the previous tile ends. No repeat-masking was performed for any of the three types of genomic regions. Data are shown for the HUES6 human ES cell line.
    Figure Legend Snippet: Genomic coverage was quantified by the number of DNA methylation measurements that overlap with CpG islands (top row), gene promoters (center row) and a 1-kilobase tiling of the genome (bottom row). For MeDIP and MethylCap, the number of measurements is equal to the number of unique sequencing reads that fall inside each region. For RRBS, it refers to the number of valid DNA methylation measurements at CpGs within each region (one RRBS sequencing read typically yields one measurement, but can also give rise to more than one measurement if it contains several CpGs). For Infinium, the number of measurements is equal to the number of CpGs within each region that are present on the HumanMethylation27 microarray. CpG islands were calculated using CgiHunter ( http://cgihunter.bioinf.mpi-inf.mpg.de/ ), requiring a minimum CpG observed vs. expected ratio of 0.6, a minimum GC content of 0.5 and a minimum length of 700 basepairs . Promoter regions were calculated based on Ensembl gene annotations, such that the region starts one kilo-base upstream of the annotated transcription start site (TSS) and extends to one kilobase downstream of the TSS. The genomic tiling was obtained by sliding a 1-kilobase window through the genome such that each tile starts at the position where the previous tile ends. No repeat-masking was performed for any of the three types of genomic regions. Data are shown for the HUES6 human ES cell line.

    Techniques Used: DNA Methylation Assay, Methylated DNA Immunoprecipitation, Sequencing, Microarray



    Similar Products

    bisulfite-based microarray methods  (INFINIUM Inc)
    90
    INFINIUM Inc bisulfite-based microarray methods
    Bisulfite Based Microarray Methods, supplied by INFINIUM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bisulfite-based+microarray+methods/pmc11742067-14-37-40?v=INFINIUM+Inc
    Average 90 stars, based on 1 article reviews
    bisulfite-based microarray methods - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results