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pspax2 addgene  (Addgene inc)


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    Addgene inc pspax2 addgene
    Pspax2 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 15453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bisulfite-based+microarray+methods/psPAX2+(Plasmid+%2312260)/pm29272703-251-320-321
    Average 98 stars, based on 15453 article reviews
    pspax2 addgene - by Bioz Stars, 2026-09
    98/100 stars

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    Produced:

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: .. Lentiviruses were produced in 293T cells by co-transfecting the shRNA plasmid with psPAX2 (Addgene, #12260) and pMD2.G (Addgene, #12259) using Lipofectamine 3000 (Invitrogen, Cat# L3000008). .. Viral supernatants were harvested at 48 h, filtered (0.45 μm), and used to infect target cells in the presence of Polybrene (Beyotime, Cat# C0351; 8 μg/mL).

    Article Title: USP20 governs tyrosine kinase inhibitors resistance through ferroptosis evasion by targeting GPX4 in cancers
    Article Snippet: .. Lentiviral particles were produced in HEK293T cells by co-transfecting pLKO.1-puro constructs with the packaging plasmid psPAX2 (Addgene #12260) and the envelope plasmid pMD2.G (Addgene #12259) at a ratio of 6:3:1.5 using polyethylenimine (PEI, 25 kDa, Merck/Sigma-Aldrich, Cat. No. 408727). ..

    shRNA:

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: .. Lentiviruses were produced in 293T cells by co-transfecting the shRNA plasmid with psPAX2 (Addgene, #12260) and pMD2.G (Addgene, #12259) using Lipofectamine 3000 (Invitrogen, Cat# L3000008). .. Viral supernatants were harvested at 48 h, filtered (0.45 μm), and used to infect target cells in the presence of Polybrene (Beyotime, Cat# C0351; 8 μg/mL).

    Plasmid Preparation:

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: .. Lentiviruses were produced in 293T cells by co-transfecting the shRNA plasmid with psPAX2 (Addgene, #12260) and pMD2.G (Addgene, #12259) using Lipofectamine 3000 (Invitrogen, Cat# L3000008). .. Viral supernatants were harvested at 48 h, filtered (0.45 μm), and used to infect target cells in the presence of Polybrene (Beyotime, Cat# C0351; 8 μg/mL).

    Article Title: ACSBG1-driven lipid reprogramming unveiled: A fatty acid metabolism-associated prognostic model for lung adenocarcinoma
    Article Snippet: For ACSBG1 overexpression (OE) and knockdown (KD), the pLenti-CMV-Puro vector (VectorBuilder Inc.) was used. .. The third-generation lentiviral packaging system was employed, consisting of the packaging plasmid psPAX2 and the envelope plasmid pMD2.G (Addgene, Inc.). .. Lentiviral particles were produced by co-transfecting 293T cells (CRL-3216TM; American Type Culture Collection) using Lipofectamine ® 3000 (Invitrogen; Thermo Fisher Scientific, Inc.).

    Article Title: PRAS40 activates the IRE1α-XBP-1-mediated unfolded protein response to exacerbate colorectal cancer by enhancing ST6Gal1-dependent α-2, 6 sialylation of GRP78
    Article Snippet: .. Virus particles were harvested 48 h after expression vector transfection with packaging plasmid psPAX2 (a gift from Dr. Didier Trono, Addgene plasmid #12260) and envelope plasmid pMD2.G (a gift from Dr. Didier Trono, Addgene plasmid #12259) into HEK293T cells. ..

    Article Title: New selective and allosteric FLT3 inhibitors show efficacy against resistant acute myeloid leukemia cells
    Article Snippet: .. psPAX2 , Addgene , Plasmid #12260. .. pMD2.G , Addgene , Plasmid #12259.

    Article Title: Hexaraphane as a potential therapeutic strategy for tauopathies
    Article Snippet: .. Briefly, a transfection mixture containing 6 μg of envelope plasmid (pMD2G, Addgene #12259), 6 μg of packaging plasmid (psPAX2, Addgene #12260), and 10 μg of the transfer vector was prepared in DMEM (Sigma-Aldrich). .. This plasmid combination was introduced into HEK293T cells (2 × 10 6 cells per 100 mm dish) using Lipofectamine (Invitrogen, 18324-012) together with Plus Reagent (Invitrogen, 11514-015).

    Article Title: circOMA1 delivered by exosomes regulates DRD2-mediated prolactinoma resistance
    Article Snippet: .. The constructed target plasmid LentiCRISPRv2 and packaging plasmids psPAX2 (cat. no. 12260; Addgene, Inc.) and pMD2.G (cat. no. 12259; Addgene, Inc.) were transfected using Liposome ® 3000 with a ratio of 5 μ g: 5 μ g: 5 μ g. After 48 h of transfection, the supernatant containing lentiviral particles (generation system used, 2nd; MOI used to infect cells, 20) from 293T cells was collected and filtered through a 0.45- μ m filter (MilliporeSigma) for subsequent MMQ cell infection. ..

    Article Title: USP20 governs tyrosine kinase inhibitors resistance through ferroptosis evasion by targeting GPX4 in cancers
    Article Snippet: .. Lentiviral particles were produced in HEK293T cells by co-transfecting pLKO.1-puro constructs with the packaging plasmid psPAX2 (Addgene #12260) and the envelope plasmid pMD2.G (Addgene #12259) at a ratio of 6:3:1.5 using polyethylenimine (PEI, 25 kDa, Merck/Sigma-Aldrich, Cat. No. 408727). ..

    Virus:

    Article Title: PRAS40 activates the IRE1α-XBP-1-mediated unfolded protein response to exacerbate colorectal cancer by enhancing ST6Gal1-dependent α-2, 6 sialylation of GRP78
    Article Snippet: .. Virus particles were harvested 48 h after expression vector transfection with packaging plasmid psPAX2 (a gift from Dr. Didier Trono, Addgene plasmid #12260) and envelope plasmid pMD2.G (a gift from Dr. Didier Trono, Addgene plasmid #12259) into HEK293T cells. ..

    Expressing:

    Article Title: PRAS40 activates the IRE1α-XBP-1-mediated unfolded protein response to exacerbate colorectal cancer by enhancing ST6Gal1-dependent α-2, 6 sialylation of GRP78
    Article Snippet: .. Virus particles were harvested 48 h after expression vector transfection with packaging plasmid psPAX2 (a gift from Dr. Didier Trono, Addgene plasmid #12260) and envelope plasmid pMD2.G (a gift from Dr. Didier Trono, Addgene plasmid #12259) into HEK293T cells. ..

    Transfection:

    Article Title: PRAS40 activates the IRE1α-XBP-1-mediated unfolded protein response to exacerbate colorectal cancer by enhancing ST6Gal1-dependent α-2, 6 sialylation of GRP78
    Article Snippet: .. Virus particles were harvested 48 h after expression vector transfection with packaging plasmid psPAX2 (a gift from Dr. Didier Trono, Addgene plasmid #12260) and envelope plasmid pMD2.G (a gift from Dr. Didier Trono, Addgene plasmid #12259) into HEK293T cells. ..

    Article Title: Hexaraphane as a potential therapeutic strategy for tauopathies
    Article Snippet: .. Briefly, a transfection mixture containing 6 μg of envelope plasmid (pMD2G, Addgene #12259), 6 μg of packaging plasmid (psPAX2, Addgene #12260), and 10 μg of the transfer vector was prepared in DMEM (Sigma-Aldrich). .. This plasmid combination was introduced into HEK293T cells (2 × 10 6 cells per 100 mm dish) using Lipofectamine (Invitrogen, 18324-012) together with Plus Reagent (Invitrogen, 11514-015).

    Article Title: circOMA1 delivered by exosomes regulates DRD2-mediated prolactinoma resistance
    Article Snippet: .. The constructed target plasmid LentiCRISPRv2 and packaging plasmids psPAX2 (cat. no. 12260; Addgene, Inc.) and pMD2.G (cat. no. 12259; Addgene, Inc.) were transfected using Liposome ® 3000 with a ratio of 5 μ g: 5 μ g: 5 μ g. After 48 h of transfection, the supernatant containing lentiviral particles (generation system used, 2nd; MOI used to infect cells, 20) from 293T cells was collected and filtered through a 0.45- μ m filter (MilliporeSigma) for subsequent MMQ cell infection. ..

    Construct:

    Article Title: circOMA1 delivered by exosomes regulates DRD2-mediated prolactinoma resistance
    Article Snippet: .. The constructed target plasmid LentiCRISPRv2 and packaging plasmids psPAX2 (cat. no. 12260; Addgene, Inc.) and pMD2.G (cat. no. 12259; Addgene, Inc.) were transfected using Liposome ® 3000 with a ratio of 5 μ g: 5 μ g: 5 μ g. After 48 h of transfection, the supernatant containing lentiviral particles (generation system used, 2nd; MOI used to infect cells, 20) from 293T cells was collected and filtered through a 0.45- μ m filter (MilliporeSigma) for subsequent MMQ cell infection. ..

    Article Title: USP20 governs tyrosine kinase inhibitors resistance through ferroptosis evasion by targeting GPX4 in cancers
    Article Snippet: .. Lentiviral particles were produced in HEK293T cells by co-transfecting pLKO.1-puro constructs with the packaging plasmid psPAX2 (Addgene #12260) and the envelope plasmid pMD2.G (Addgene #12259) at a ratio of 6:3:1.5 using polyethylenimine (PEI, 25 kDa, Merck/Sigma-Aldrich, Cat. No. 408727). ..

    Infection:

    Article Title: circOMA1 delivered by exosomes regulates DRD2-mediated prolactinoma resistance
    Article Snippet: .. The constructed target plasmid LentiCRISPRv2 and packaging plasmids psPAX2 (cat. no. 12260; Addgene, Inc.) and pMD2.G (cat. no. 12259; Addgene, Inc.) were transfected using Liposome ® 3000 with a ratio of 5 μ g: 5 μ g: 5 μ g. After 48 h of transfection, the supernatant containing lentiviral particles (generation system used, 2nd; MOI used to infect cells, 20) from 293T cells was collected and filtered through a 0.45- μ m filter (MilliporeSigma) for subsequent MMQ cell infection. ..

    Sequencing:

    Article Title: Depleting HIF-1α attenuates the progression of osteosarcoma, but tumorigenicity is sustained through HIF-independent pathways
    Article Snippet: .. After confirmation of the inserted sequence, the lentiCRISPRv2-puro vectors were co-transfected along with the psPAX2 (cat. no. 12260; Addgene, Inc.) and pCMV–VSV-G (Addgene_8454; Addgene, Inc.) vectors into Lenti-X 293T cells (CVCL_4401; Takara Bio) using FuGENE HD (Promega Corporation) to generate infectious lentivirus. ..



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