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binding buffer xbp  (Qiagen)


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    Structured Review

    Qiagen binding buffer xbp
    Binding Buffer Xbp, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/binding+buffer/Buffer+XBP/us12624348-843-33-40
    Average 94 stars, based on 18 article reviews
    binding buffer xbp - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: Plasma-derived exosomal long noncoding RNAs of pancreatic cancer patients as novel blood-based biomarkers of disease
    Article Snippet: .. Briefly, 1 mL of prefiltered (0.8 μm) plasma from patients was mixed with 2 × binding buffer (XBP) and added to an exoEasy membrane affinity rotation column (QIAGEN, Cat. 77,044). ..

    Article Title: Enrichment method
    Article Snippet: .. For each condition tested (in duplicate) 1 ml of pooled plasma was mixed with 1 volume of the different 2× citrate binding buffers shown in table 2 above or 1 volume of the binding buffer XBP from the exoEasy/exoRNeasy kit (QIAGEN) and inverted 5 times. ..

    Article Title: Extracellular vesicles as a potential source of tumor-derived DNA in advanced pancreatic cancer.
    Article Snippet: Eluted fractions (containing sEVs) were combined and concentrated using Amicon1 Ultra-15 Centrifugal Filter Units (100 kDa, Merck) and subjected directly to downstream analyses. exoEasy membrane affinity spin columns Isolation of sEVs using membrane affinity spin columns was performed according to the manufacturer’s instructions (exoEasy Maxi Kit, Qiagen). .. Briefly, one volume of XBP buffer (Qiagen) was added to clarified plasma and the sample then added to a spin column and centrifuged at 500 × g for 1 min. Next, 10 mL XWP buffer (Qiagen) was added to the spin column and the sample centrifuged at 5000 × g for 5 min. .. Finally, the sEVs were eluted in 400 μL buffer XE (Qiagen) by sample centrifugation at 500 × g for 5 min, re-application of the eluate to the spin column, and centrifugation at 5000 × g for 5 min.

    Article Title: Plasma-derived exosomal long noncoding RNAs of pancreatic cancer patients as novel blood-based biomarkers of disease.
    Article Snippet: .. Briefly, 1 mL of prefiltered (0.8 μm) plasma from patients was mixed with 2 × binding buffer (XBP) and added to an exoEasy membrane affinity rotation column (QIAGEN, Cat. 77,044). ..

    Binding Assay:

    Article Title: Plasma-derived exosomal long noncoding RNAs of pancreatic cancer patients as novel blood-based biomarkers of disease
    Article Snippet: .. Briefly, 1 mL of prefiltered (0.8 μm) plasma from patients was mixed with 2 × binding buffer (XBP) and added to an exoEasy membrane affinity rotation column (QIAGEN, Cat. 77,044). ..

    Article Title: FAM84B facilitates tau propagation via RYR3-mediated exocytosis in response to neuroinflammation
    Article Snippet: The membrane affinity-based exosome isolation process began with filtering the conditioned media through a 0.45 μm syringe filter (Pall Corporation, New York, NY, USA). .. The filtered media were then mixed with a binding buffer (buffer XBP) and loaded onto the membrane spin column of the ExoEasy Maxi Kit (Qiagen, Venlo, the Netherlands). ..

    Article Title: Enrichment method
    Article Snippet: .. For each condition tested (in duplicate) 1 ml of pooled plasma was mixed with 1 volume of the different 2× citrate binding buffers shown in table 2 above or 1 volume of the binding buffer XBP from the exoEasy/exoRNeasy kit (QIAGEN) and inverted 5 times. ..

    Article Title: Plasma-derived exosomal long noncoding RNAs of pancreatic cancer patients as novel blood-based biomarkers of disease.
    Article Snippet: .. Briefly, 1 mL of prefiltered (0.8 μm) plasma from patients was mixed with 2 × binding buffer (XBP) and added to an exoEasy membrane affinity rotation column (QIAGEN, Cat. 77,044). ..

    Membrane:

    Article Title: Plasma-derived exosomal long noncoding RNAs of pancreatic cancer patients as novel blood-based biomarkers of disease
    Article Snippet: .. Briefly, 1 mL of prefiltered (0.8 μm) plasma from patients was mixed with 2 × binding buffer (XBP) and added to an exoEasy membrane affinity rotation column (QIAGEN, Cat. 77,044). ..

    Article Title: FAM84B facilitates tau propagation via RYR3-mediated exocytosis in response to neuroinflammation
    Article Snippet: The membrane affinity-based exosome isolation process began with filtering the conditioned media through a 0.45 μm syringe filter (Pall Corporation, New York, NY, USA). .. The filtered media were then mixed with a binding buffer (buffer XBP) and loaded onto the membrane spin column of the ExoEasy Maxi Kit (Qiagen, Venlo, the Netherlands). ..

    Article Title: Plasma-derived exosomal long noncoding RNAs of pancreatic cancer patients as novel blood-based biomarkers of disease.
    Article Snippet: .. Briefly, 1 mL of prefiltered (0.8 μm) plasma from patients was mixed with 2 × binding buffer (XBP) and added to an exoEasy membrane affinity rotation column (QIAGEN, Cat. 77,044). ..



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    Image Search Results


    CRISPR/Cas9 induces DNA damage and apoptosis in PGCs. (A) Flow cytometry analysis 24 h after electroporation, quantifying the proportion of Annexin V + /PI + cells. The horizontal axis indicates PI and the vertical axis Annexin V. The upper-left quadrant (Annexin V + /PI + ) represents late apoptotic cells, and the lower-right quadrant (Annexin V + only) represents early apoptotic cells. Upper panels: results after electroporation with Cas9 + various gRNAs; lower panels: results with dCas9 + various gRNAs. (B) Bar graph of Annexin V + /PI + percentages across groups. Cas9 editing induced a highly significant increase in late apoptosis. (C) γ-H 2 AX foci (green) detected by immunofluorescence 24 h after electroporation. Foci appear as discrete nuclear puncta; nuclei are counterstained with DAPI (blue). Scale bar = 10 µm. (D) Quantification of γ-H 2 AX foci per cell. Cas9 targeting resulted in a significant increase in γ-H 2 AX foci per cell, whereas dCas9 with sgRNA did not. Statistical significance determined by one-way ANOVA (p-values are indicated in the figure).

    Journal: Poultry Science

    Article Title: High genotoxicity of CRISPR/Cas9 versus limited efficacy of CRISPRi in chicken primordial germ cells

    doi: 10.1016/j.psj.2026.106722

    Figure Lengend Snippet: CRISPR/Cas9 induces DNA damage and apoptosis in PGCs. (A) Flow cytometry analysis 24 h after electroporation, quantifying the proportion of Annexin V + /PI + cells. The horizontal axis indicates PI and the vertical axis Annexin V. The upper-left quadrant (Annexin V + /PI + ) represents late apoptotic cells, and the lower-right quadrant (Annexin V + only) represents early apoptotic cells. Upper panels: results after electroporation with Cas9 + various gRNAs; lower panels: results with dCas9 + various gRNAs. (B) Bar graph of Annexin V + /PI + percentages across groups. Cas9 editing induced a highly significant increase in late apoptosis. (C) γ-H 2 AX foci (green) detected by immunofluorescence 24 h after electroporation. Foci appear as discrete nuclear puncta; nuclei are counterstained with DAPI (blue). Scale bar = 10 µm. (D) Quantification of γ-H 2 AX foci per cell. Cas9 targeting resulted in a significant increase in γ-H 2 AX foci per cell, whereas dCas9 with sgRNA did not. Statistical significance determined by one-way ANOVA (p-values are indicated in the figure).

    Article Snippet: After another centrifugation, the supernatant was removed and cells were resuspended in 500 μL of 1× Annexin V Binding Buffer (from Annexin V-FITC/PI Apoptosis Kit, Elabscience E-CK-A211).

    Techniques: CRISPR, Flow Cytometry, Electroporation, Immunofluorescence

    PGCs are more sensitive to DNA double-strand breaks than somatic cells. (A) Cell viability curves after 24 h treatment with increasing concentrations of etoposide (ETP; x-axis shows log 10 [ETP]). PGC viability dropped sharply even at low ETP doses, whereas CEF cells were more tolerant (viability data are mean ± SD of triplicates). (B) Apoptosis detection in PGCs treated with low-dose ETP. The proportion of Annexin V + /PI + cells (late apoptosis) was significantly elevated even at 0.03 µM ETP. (C) Western blot analysis of γ-H 2 AX protein in PGCs treated with varying ETP concentrations (left panel; β-actin as loading control). The bar graph (right) shows the ratio of γ-H 2 AX to β-actin band intensity, with a marked increase at 3 µM ETP (p-values are indicated in the figure). (D) Quantification of γ-H 2 AX foci per cell in THP-1 cells, male PGCs, and female PGCs after exposure to X-ray doses of 0, 2, 4, 6, and 8 Gy. After 48 h recovery, γ-H 2 AX foci increased significantly with higher radiation in THP-1, male PGCs, and female PGCs. Female PGC data points are red squares; male PGCs are blue squares; THP-1 are black circles. (E) Cell-cycle distribution of male vs. female PGCs after DNA damage. PGCs were irradiated (2, 4, 6 Gy), cultured 48 h, and analyzed by flow cytometry for cell-cycle phase (propidium iodide staining). Stacked bars show the percentage of cells in G 0 G 1 , S, and G 2 /M phases in untreated vs. irradiated cells. After damage, female PGCs predominantly arrested in G 2 /M (increased G 2 fraction), whereas male PGCs accumulated in S phase. Statistical significance by one-way ANOVA (p-values are indicated in the figure).

    Journal: Poultry Science

    Article Title: High genotoxicity of CRISPR/Cas9 versus limited efficacy of CRISPRi in chicken primordial germ cells

    doi: 10.1016/j.psj.2026.106722

    Figure Lengend Snippet: PGCs are more sensitive to DNA double-strand breaks than somatic cells. (A) Cell viability curves after 24 h treatment with increasing concentrations of etoposide (ETP; x-axis shows log 10 [ETP]). PGC viability dropped sharply even at low ETP doses, whereas CEF cells were more tolerant (viability data are mean ± SD of triplicates). (B) Apoptosis detection in PGCs treated with low-dose ETP. The proportion of Annexin V + /PI + cells (late apoptosis) was significantly elevated even at 0.03 µM ETP. (C) Western blot analysis of γ-H 2 AX protein in PGCs treated with varying ETP concentrations (left panel; β-actin as loading control). The bar graph (right) shows the ratio of γ-H 2 AX to β-actin band intensity, with a marked increase at 3 µM ETP (p-values are indicated in the figure). (D) Quantification of γ-H 2 AX foci per cell in THP-1 cells, male PGCs, and female PGCs after exposure to X-ray doses of 0, 2, 4, 6, and 8 Gy. After 48 h recovery, γ-H 2 AX foci increased significantly with higher radiation in THP-1, male PGCs, and female PGCs. Female PGC data points are red squares; male PGCs are blue squares; THP-1 are black circles. (E) Cell-cycle distribution of male vs. female PGCs after DNA damage. PGCs were irradiated (2, 4, 6 Gy), cultured 48 h, and analyzed by flow cytometry for cell-cycle phase (propidium iodide staining). Stacked bars show the percentage of cells in G 0 G 1 , S, and G 2 /M phases in untreated vs. irradiated cells. After damage, female PGCs predominantly arrested in G 2 /M (increased G 2 fraction), whereas male PGCs accumulated in S phase. Statistical significance by one-way ANOVA (p-values are indicated in the figure).

    Article Snippet: After another centrifugation, the supernatant was removed and cells were resuspended in 500 μL of 1× Annexin V Binding Buffer (from Annexin V-FITC/PI Apoptosis Kit, Elabscience E-CK-A211).

    Techniques: Western Blot, Control, Irradiation, Cell Culture, Flow Cytometry, Staining