buffer (Miltenyi Biotec)
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Miltenyi Biotec
buffer
Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/binding+buffer/MACS+ART+Binding+Buffer/pmc06219306-62-30-35
Average 96 stars, based on 3 article reviews
Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/binding+buffer/MACS+ART+Binding+Buffer/pmc06219306-62-30-35
Average 96 stars, based on 3 article reviews
buffer - by Bioz Stars,
2026-09
96/100 stars
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Binding Assay:Article Title: Human brain cell types shape host-rabies virus transcriptional interactions revealing a preexisting pro-viral astrocyte subpopulation Article Snippet: Samples were added to the freshly washed LS columns (130-042-401, Miltenyi Biotec). .. Columns were washed with 4.5 mL Article Title: Transmembrane thiol isomerase TMX1 inhibits fibrin formation by down-regulation of platelet phosphatidylserine exposure Article Snippet: .. The cell pellet was suspended with 90 μl Article Title: An optimised Bcl-3 inhibitor for melanoma treatment. Article Snippet: Input was saved before samples were incubated for 30 min at 4 C with rotation with GSTmicrobeads (130-091-370 kit, Miltenyi Biotec). μMACS columns (Miltenyi Biotec, Bergisch Gladbach, Germany) were placed in the separator and prepared with binding buffer. .. Samples were run through the columns and washed 2 with Article Title: Human brain cell types shape host-rabies virus transcriptional interactions revealing a preexisting pro-viral astrocyte subpopulation. Article Snippet: Samples were added to the freshly washed LS columns (130-042-401, Miltenyi Biotec). .. Columns were washed with 4.5 mL Article Title: Monocyte and macrophage binding aptamers and their application Article Snippet: Binding buffer: wash buffer+0.5% BSA (Miltenyi)+0.1 mg/mL tRNA (Invitrogen) *Avoid EDTA in the system .. Article Title: Functional phenotyping of genomic variants using joint multiomic single-cell DNA-RNA sequencing. Article Snippet: Cells were resuspended in 100 μl of bead solution from a Dead Cell Removal kit (Miltenyi Biotec, 130-090- 101) and incubated for 15 min in the dark. .. Article Title: Transmembrane thiol isomerase TMX1 inhibits fibrin formation by down-regulation of platelet phosphatidylserine exposure. Article Snippet: .. The cell pellet was suspended with 90 μl Western Blot:Article Title: An optimised Bcl-3 inhibitor for melanoma treatment. Article Snippet: Input was saved before samples were incubated for 30 min at 4 C with rotation with GSTmicrobeads (130-091-370 kit, Miltenyi Biotec). μMACS columns (Miltenyi Biotec, Bergisch Gladbach, Germany) were placed in the separator and prepared with binding buffer. .. Samples were run through the columns and washed 2 with other:Article Title: Unveiling IRF4-steered regulation of context-dependent effector programs in CD4 + T cells under Th17- and Treg-skewing conditions. Article Snippet: 24 Cell Reports 44, 115407, March 25, 2025 anti-CD44 (BioLegend). |
![PGCs are more sensitive to DNA double-strand breaks than somatic cells. (A) Cell viability curves after 24 h treatment with increasing concentrations of etoposide (ETP; x-axis shows log 10 [ETP]). PGC viability dropped sharply even at low ETP doses, whereas CEF cells were more tolerant (viability data are mean ± SD of triplicates). (B) Apoptosis detection in PGCs treated with low-dose ETP. The proportion of Annexin V + /PI + cells (late apoptosis) was significantly elevated even at 0.03 µM ETP. (C) Western blot analysis of γ-H 2 AX protein in PGCs treated with varying ETP concentrations (left panel; β-actin as loading control). The bar graph (right) shows the ratio of γ-H 2 AX to β-actin band intensity, with a marked increase at 3 µM ETP (p-values are indicated in the figure). (D) Quantification of γ-H 2 AX foci per cell in THP-1 cells, male PGCs, and female PGCs after exposure to X-ray doses of 0, 2, 4, 6, and 8 Gy. After 48 h recovery, γ-H 2 AX foci increased significantly with higher radiation in THP-1, male PGCs, and female PGCs. Female PGC data points are red squares; male PGCs are blue squares; THP-1 are black circles. (E) Cell-cycle distribution of male vs. female PGCs after DNA damage. PGCs were irradiated (2, 4, 6 Gy), cultured 48 h, and analyzed by flow cytometry for cell-cycle phase (propidium iodide staining). Stacked bars show the percentage of cells in G 0 G 1 , S, and G 2 /M phases in untreated vs. irradiated cells. After damage, female PGCs predominantly arrested in G 2 /M (increased G 2 fraction), whereas male PGCs accumulated in S phase. Statistical significance by one-way ANOVA (p-values are indicated in the figure).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1053/pmc13011053/pmc13011053__gr3.jpg)