binding buffer (MACHEREY NAGEL)
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binding buffer
Binding Buffer, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/binding+buffer/Binding+Buffer+PC/pmc04646318-248-3-37
Average 90 stars, based on 5 article reviews
Binding Buffer, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/binding+buffer/Binding+Buffer+PC/pmc04646318-248-3-37
Average 90 stars, based on 5 article reviews
binding buffer - by Bioz Stars,
2026-09
90/100 stars
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Binding Assay:Article Title: Variants of PpuLcc, a multi-dye decolorizing laccase from Pleurotus pulmonarius expressed in Pichia pastoris. Article Snippet: 9 A laccase of the basidiomycete Pleurotus pulmonarius (PpuLcc) possessed strong 10 decolorizing abilities towards artificial and natural dyes.. The PpuLcc was purified from the 11 culture supernatant via FPLC, and the corresponding gene cloned and expressed in 12 Pichia pastoris GS115.. To examine the impact of the C-terminal tail region and the signal 13 peptide on the recombinant expression of PpuLcc, a non-modified version or different 14 truncations (-2, -5, -13 AA) of the target protein were combined with different secretion 15 signals. Article Title: Structural Insights into Cargo Recognition by the Yeast PTS1 Receptor Article Snippet: The supernatant contained the soluble His 6 -Pex5p or His 6 -Pex5p-M536A and was loaded onto a nickel-NTA column (HisTrap HP, GE Healthcare) of an ÄKTAprime TM system (GE Healthcare). .. After washing with Article Title: qPCR-based relative quantification of the brown algal endophyte Laminarionema elsbetiae in Saccharina latissima : variation and dynamics of host—endophyte interactions Article Snippet: One hundred microliter lysozyme (20 mg mL −1 ) was added, and samples were shaken at 37 °C for 45 min. Twenty microliter proteinase K (20 mg mL −1 ) and 100 μL 20% SDS was added, and samples were homogenised by inversion and incubated at 55 °C for 1 h. The content was transferred to a new tube and 1 vol phenol-chloroform-isoamyl alcohol (25:24:1) was added; the phases were mixed and then centrifuged for 15 min at 4500 rpm at 4 °C. .. The supernatant was mixed with 1 vol of the Article Title: Structural organizations of yeast RNase P and RNase MRP holoenzymes as revealed by UV-crosslinking studies of RNA-protein interactions Article Snippet: .. The samples containing disassembled RNases P/MRP were transferred in 1 mL of a Derivative Assay:Article Title: Structural Insights into Cargo Recognition by the Yeast PTS1 Receptor Article Snippet: The supernatant contained the soluble His 6 -Pex5p or His 6 -Pex5p-M536A and was loaded onto a nickel-NTA column (HisTrap HP, GE Healthcare) of an ÄKTAprime TM system (GE Healthcare). .. After washing with Expressing:Article Title: Structural Insights into Cargo Recognition by the Yeast PTS1 Receptor Article Snippet: The supernatant contained the soluble His 6 -Pex5p or His 6 -Pex5p-M536A and was loaded onto a nickel-NTA column (HisTrap HP, GE Healthcare) of an ÄKTAprime TM system (GE Healthcare). .. After washing with Incubation:Article Title: Structural organizations of yeast RNase P and RNase MRP holoenzymes as revealed by UV-crosslinking studies of RNA-protein interactions Article Snippet: .. The samples containing disassembled RNases P/MRP were transferred in 1 mL of a |
![PGCs are more sensitive to DNA double-strand breaks than somatic cells. (A) Cell viability curves after 24 h treatment with increasing concentrations of etoposide (ETP; x-axis shows log 10 [ETP]). PGC viability dropped sharply even at low ETP doses, whereas CEF cells were more tolerant (viability data are mean ± SD of triplicates). (B) Apoptosis detection in PGCs treated with low-dose ETP. The proportion of Annexin V + /PI + cells (late apoptosis) was significantly elevated even at 0.03 µM ETP. (C) Western blot analysis of γ-H 2 AX protein in PGCs treated with varying ETP concentrations (left panel; β-actin as loading control). The bar graph (right) shows the ratio of γ-H 2 AX to β-actin band intensity, with a marked increase at 3 µM ETP (p-values are indicated in the figure). (D) Quantification of γ-H 2 AX foci per cell in THP-1 cells, male PGCs, and female PGCs after exposure to X-ray doses of 0, 2, 4, 6, and 8 Gy. After 48 h recovery, γ-H 2 AX foci increased significantly with higher radiation in THP-1, male PGCs, and female PGCs. Female PGC data points are red squares; male PGCs are blue squares; THP-1 are black circles. (E) Cell-cycle distribution of male vs. female PGCs after DNA damage. PGCs were irradiated (2, 4, 6 Gy), cultured 48 h, and analyzed by flow cytometry for cell-cycle phase (propidium iodide staining). Stacked bars show the percentage of cells in G 0 G 1 , S, and G 2 /M phases in untreated vs. irradiated cells. After damage, female PGCs predominantly arrested in G 2 /M (increased G 2 fraction), whereas male PGCs accumulated in S phase. Statistical significance by one-way ANOVA (p-values are indicated in the figure).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1053/pmc13011053/pmc13011053__gr3.jpg)