annexin binding buffer (Fisher Scientific)
86
Structured Review
Fisher Scientific
annexin binding buffer
Annexin Binding Buffer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/binding+buffer/binding+low+protein+tube/pm42275220-383-7-13
Average 86 stars, based on 1 article reviews
Annexin Binding Buffer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/binding+buffer/binding+low+protein+tube/pm42275220-383-7-13
Average 86 stars, based on 1 article reviews
annexin binding buffer - by Bioz Stars,
2026-11
86/100 stars
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Protein Binding:Article Title: Mechanical Flexibility Enables DNA Origami to Overcome Steric Confinement in Mucus Article Snippet: .. For Centrifugation:Article Title: Loading Density Influences the Tumor Cell Targeting and Signaling Inhibition Capabilities of Antibody Nanoconjugates Article Snippet: .. The FZD7-NS and PEG-NS were separated from unbound OPSS-PEG-FZD7 and mPEG-SH by centrifuging at 500 g for 15 min once, followed by two 10 min centrifugation steps at 500 g, all in Article Title: Loading Density Influences the Tumor Cell Targeting and Signaling Inhibition Capabilities of Antibody Nanoconjugates Article Snippet: .. The FZD7-NS and PEG-NS were separated from unbound OPSS-PEG-FZD7 and mPEG-SH by centrifuging at 500 g for 15 min once, followed by two 10 min centrifugation steps at 500 g, all in Low Protein Binding:Article Title: Loading Density Influences the Tumor Cell Targeting and Signaling Inhibition Capabilities of Antibody Nanoconjugates Article Snippet: .. The FZD7-NS and PEG-NS were separated from unbound OPSS-PEG-FZD7 and mPEG-SH by centrifuging at 500 g for 15 min once, followed by two 10 min centrifugation steps at 500 g, all in Article Title: Cortical cell culture model for examining cancer extracellular vesicle dynamics and neuroinflammatory response. Article Snippet: .. EVs were then aliquoted at 1x1011 particles/tube in low Article Title: Cortical cell culture model for examining cancer extracellular vesicle dynamics and neuroinflammatory response Article Snippet: .. EVs were then aliquoted at 1 × 10 11 particles/tube in low Article Title: Loading Density Influences the Tumor Cell Targeting and Signaling Inhibition Capabilities of Antibody Nanoconjugates Article Snippet: .. The FZD7-NS and PEG-NS were separated from unbound OPSS-PEG-FZD7 and mPEG-SH by centrifuging at 500 g for 15 min once, followed by two 10 min centrifugation steps at 500 g, all in Binding Assay:Article Title: Cell size-dependent mRNA transcription drives proteome remodeling. Article Snippet: Cells were then stained with LIVE/DEAD 405 marker (Thermo L34955; 0.1 μL per 100 k cells) and Annexin V 647 conjugate (Thermo A23204; 5 μL per 100 k cells) in Annexin binding buffer (Thermo; V13246) at room temperature for 30 min. .. Afterward, the cells were washed once with Incubation:Article Title: Reduced RhoGDI2 Expression Disrupts Centrosome Functions and Promotes Mitotic Errors Article Snippet: .. The coverslips were then washed 3× in Article Title: In vivo multiomic Perturb-seq with enhanced nuclear gRNA capture Article Snippet: The nuclei pellet was resuspended in washing buffer and incubated with anti-NeuN–AF647 antibody (Abcam, ab190565, 1:500) and mouse nucleoporin P62 sample tag antibody (BD, 460293-460297, 1:200, only for 16-gene screen) for 15 min on ice. .. For nuclei isolation of HT22 cells, cells were washed twice with cold DPBS, scraped with 5mL lysis buffer (nuclei isolation medium supplemented with 1 mM DTT, 0.2% Tween-20, 0.2% IGEPAL CA-630, 1% BSA and 0.6 U/μL RNase inhibitor (Fisher Scientific, #NC1081844)), and incubated on ice for 5 min, diluted with Isolation:Article Title: In vivo multiomic Perturb-seq with enhanced nuclear gRNA capture Article Snippet: The nuclei pellet was resuspended in washing buffer and incubated with anti-NeuN–AF647 antibody (Abcam, ab190565, 1:500) and mouse nucleoporin P62 sample tag antibody (BD, 460293-460297, 1:200, only for 16-gene screen) for 15 min on ice. .. For nuclei isolation of HT22 cells, cells were washed twice with cold DPBS, scraped with 5mL lysis buffer (nuclei isolation medium supplemented with 1 mM DTT, 0.2% Tween-20, 0.2% IGEPAL CA-630, 1% BSA and 0.6 U/μL RNase inhibitor (Fisher Scientific, #NC1081844)), and incubated on ice for 5 min, diluted with Lysis:Article Title: In vivo multiomic Perturb-seq with enhanced nuclear gRNA capture Article Snippet: The nuclei pellet was resuspended in washing buffer and incubated with anti-NeuN–AF647 antibody (Abcam, ab190565, 1:500) and mouse nucleoporin P62 sample tag antibody (BD, 460293-460297, 1:200, only for 16-gene screen) for 15 min on ice. .. For nuclei isolation of HT22 cells, cells were washed twice with cold DPBS, scraped with 5mL lysis buffer (nuclei isolation medium supplemented with 1 mM DTT, 0.2% Tween-20, 0.2% IGEPAL CA-630, 1% BSA and 0.6 U/μL RNase inhibitor (Fisher Scientific, #NC1081844)), and incubated on ice for 5 min, diluted with |
![( A ) Coculture (1:1) of parental B16F10 cells expressing pBabe-Cerulean (blue) and B16F10 cells expressing GsdmD-C3 and mCherry (red) (see movie S1). ( B ) Times of individual cell shrinkage and cell membrane lysis, as in (A). Unpaired t test. ( C ) Apoptotic phase (shrinkage-lysis interval) from (B). One-way ANOVA. ( D ) Quantification of annexin V + SIINFEKL peptide–pulsed B16F10 [as in (B) and (C)] that remains fluorescent when coincubated with activated OT-I T cells at 4 hours. Two-way ANOVA. ( E ) Quantification of SIINFEKL peptide–pulsed B16F10 cell populations with ectopic caspase-3-activatable GSDMD (GD-C3) by fluorescence signal at 24 hours of coincubation with activated OT-I T cells. ( F ) As in (E) with Gsdme. [(E) and (F)] Paired t test. ( G ) Number of GCamp6s spikes observed from SIINFEKL peptide–pulsed Neuro2a cells, coincubated with activated OT-I T cells, 10:1 T cell–to–target cell ratio. Images were taken every 2 min for a duration of 3 hours immediately following the additional of OT-I T cells, and numbers of observable GCamp6s spikes were counted and plotted (see movies S2 to S4 and fig. S5C). Unpaired t test. ( H ) Design for (I) and (J). Created in BioRender; B. Liu (2026), https://BioRender.com/j67ipmw . ( I ) Bioluminescence signal intensity of Neuro2a in C57BL/6 animals ( n = 9 animals per group). Two-way ANOVA. ( J ) Kaplan-Meyer survival curves from C57BL/6 mice injected with Neuro2a cells ( n = 9 animals per group). ( K ) Design for (L). Created in BioRender; B. Liu (2026), https://BioRender.com/j67ipmw . ( L ) Bioluminescence signal intensity of Neuro2a in C57BL/6 animals (control, n = 9 animals; Gsdme −/− , n = 8 animals). ( M ) Kaplan-Meyer survival curves from C57BL/6 mice rechallenged with Neuro2a cells [ n = 5 animals per group; log-rank (Mantel-Cox) test]. ( N ) As in (M) with B16F10 [ n = 12 animals in naïve group, 14 animals in rechallenge group; log-rank (Mantel-Cox) test]. * P < 0.05, ** P < 0.01, and **** P < 0.0001. iv, intravenous; ip, intraperitoneal.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6084/pmc13626084/pmc13626084__sciadv.aeb1137-f3.jpg)