binding buffer (Elabscience Biotechnology)
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binding buffer
Binding Buffer, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/binding+buffer/Annexin+V+Binding+Buffer/pm41371635-58-8-22
Average 95 stars, based on 95 article reviews
Binding Buffer, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/binding+buffer/Annexin+V+Binding+Buffer/pm41371635-58-8-22
Average 95 stars, based on 95 article reviews
binding buffer - by Bioz Stars,
2026-09
95/100 stars
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Binding Assay:Article Title: Agonal cell resuscitation strategy to promote tissue repair. Article Snippet: To assess necroptosis, RIPK3-OE NIH3T3 were induced by adding 30ng/ml TNF-α (SinoBiological, 50349-MNAE), 10uM Lcl-161 (MCE, HY-15518) and 100uM z-VAD-fmk (APExBIO, A1902) (TSZ) for indicated times. .. Then, cells were trypsinized, resuspended in Article Title: Agonal cell resuscitation strategy to promote tissue repair. Article Snippet: .. Then, cells were trypsinized, resuspended in Article Title: Agonal cell resuscitation strategy to promote tissue repair. Article Snippet: .. After centrifugation and one wash with PBS, the cells were resuspended in Article Title: An extracellular vesicle-mediated mitochondrial transfer network critical for testosterone synthesis Article Snippet: .. The tissues were incubated with Annexin V–APC (Elabscience) in Article Title: Network toxicology and single-cell analysis reveal key gene-mediated bisphenol a interference with granulosa cell function in polycystic ovary syndrome Article Snippet: .. Cells were centrifuged at 1000 rpm for 5 min, resuspended in 500 μL 1× Article Title: An extracellular vesicle-mediated mitochondrial transfer network critical for testosterone synthesis. Article Snippet: Testosterone production by testicular Leydig cells (LCs) in male mammals is energetically demanding and prone to mitochondrial damage.. Despite these challenges, LCs exhibit remarkable longevity and minimal turnover, suggesting the existence of specialized mechanisms that maintain LC mitochondrial homeostasis under such constrains.. Here we identify a mitochondrial transfer network between LCs and different testicular macrophage (tMac) subpopulations. Article Title: Identification and Functional Validation of PTH2R as a Therapeutic Target in Lung Adenocarcinoma. Article Snippet: After treatment, the cells were collected by centrifugation at 500 rpm for five minutes and washed twice with 1× phosphate buffer solution (PBS, Servicebio, China). .. The cells were reconstituted in 125 μL of 1× Article Title: Identification and Functional Validation of PTH2R as a Therapeutic Target in Lung Adenocarcinoma Article Snippet: After treatment, the cells were collected by centrifugation at 500 rpm for five minutes and washed twice with 1× phosphate buffer solution (PBS, Servicebio, China). .. The cells were reconstituted in 125 μL of 1× Staining:Article Title: Agonal cell resuscitation strategy to promote tissue repair. Article Snippet: To assess necroptosis, RIPK3-OE NIH3T3 were induced by adding 30ng/ml TNF-α (SinoBiological, 50349-MNAE), 10uM Lcl-161 (MCE, HY-15518) and 100uM z-VAD-fmk (APExBIO, A1902) (TSZ) for indicated times. .. Then, cells were trypsinized, resuspended in Article Title: Agonal cell resuscitation strategy to promote tissue repair. Article Snippet: .. Then, cells were trypsinized, resuspended in Article Title: Agonal cell resuscitation strategy to promote tissue repair. Article Snippet: .. After centrifugation and one wash with PBS, the cells were resuspended in Article Title: Network toxicology and single-cell analysis reveal key gene-mediated bisphenol a interference with granulosa cell function in polycystic ovary syndrome Article Snippet: .. Cells were centrifuged at 1000 rpm for 5 min, resuspended in 500 μL 1× Centrifugation:Article Title: Agonal cell resuscitation strategy to promote tissue repair. Article Snippet: .. After centrifugation and one wash with PBS, the cells were resuspended in Incubation:Article Title: An extracellular vesicle-mediated mitochondrial transfer network critical for testosterone synthesis Article Snippet: .. The tissues were incubated with Annexin V–APC (Elabscience) in Article Title: An extracellular vesicle-mediated mitochondrial transfer network critical for testosterone synthesis. Article Snippet: Testosterone production by testicular Leydig cells (LCs) in male mammals is energetically demanding and prone to mitochondrial damage.. Despite these challenges, LCs exhibit remarkable longevity and minimal turnover, suggesting the existence of specialized mechanisms that maintain LC mitochondrial homeostasis under such constrains.. Here we identify a mitochondrial transfer network between LCs and different testicular macrophage (tMac) subpopulations. |
![PGCs are more sensitive to DNA double-strand breaks than somatic cells. (A) Cell viability curves after 24 h treatment with increasing concentrations of etoposide (ETP; x-axis shows log 10 [ETP]). PGC viability dropped sharply even at low ETP doses, whereas CEF cells were more tolerant (viability data are mean ± SD of triplicates). (B) Apoptosis detection in PGCs treated with low-dose ETP. The proportion of Annexin V + /PI + cells (late apoptosis) was significantly elevated even at 0.03 µM ETP. (C) Western blot analysis of γ-H 2 AX protein in PGCs treated with varying ETP concentrations (left panel; β-actin as loading control). The bar graph (right) shows the ratio of γ-H 2 AX to β-actin band intensity, with a marked increase at 3 µM ETP (p-values are indicated in the figure). (D) Quantification of γ-H 2 AX foci per cell in THP-1 cells, male PGCs, and female PGCs after exposure to X-ray doses of 0, 2, 4, 6, and 8 Gy. After 48 h recovery, γ-H 2 AX foci increased significantly with higher radiation in THP-1, male PGCs, and female PGCs. Female PGC data points are red squares; male PGCs are blue squares; THP-1 are black circles. (E) Cell-cycle distribution of male vs. female PGCs after DNA damage. PGCs were irradiated (2, 4, 6 Gy), cultured 48 h, and analyzed by flow cytometry for cell-cycle phase (propidium iodide staining). Stacked bars show the percentage of cells in G 0 G 1 , S, and G 2 /M phases in untreated vs. irradiated cells. After damage, female PGCs predominantly arrested in G 2 /M (increased G 2 fraction), whereas male PGCs accumulated in S phase. Statistical significance by one-way ANOVA (p-values are indicated in the figure).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1053/pmc13011053/pmc13011053__gr3.jpg)