ntb binding buffer (MACHEREY NAGEL)
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MACHEREY NAGEL
ntb binding buffer
Ntb Binding Buffer, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/binding+buffer/Binding+Buffer+NTB/pm37289084-628-135-141
Average 94 stars, based on 23 article reviews
Ntb Binding Buffer, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/binding+buffer/Binding+Buffer+NTB/pm37289084-628-135-141
Average 94 stars, based on 23 article reviews
ntb binding buffer - by Bioz Stars,
2026-10
94/100 stars
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Purification:Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA. Article Snippet: .. Samples were resuspended in two volumes of Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA Article Snippet: .. Samples were resuspended in two volumes of Article Title: TERRA R-loops trigger a switch in telomere maintenance towards break-induced replication and PRIMPOL-dependent repair Article Snippet: Washed beads and input samples were resuspended in crosslink reversal buffer (20 mM Tris-HCl pH 8.0, 0.1% SDS, 0.1 M NaHCO3, 0.5 mM EDTA) supplemented with 10 μg/mL RNase (DNase-free (Roche)) and incubated at 65 °C overnight. .. DNA was purified using the NucleoSpin Gel and PCR Clean-up kit with Article Title: TERRA R-loops trigger a switch in telomere maintenance towards break-induced replication and PRIMPOL-dependent repair. Article Snippet: Washed beads and input samples were resuspended in crosslink reversal buffer (20 mM Tris-HCl pH 8.0, 0.1% SDS, 0.1 M NaHCO3, 0.5 mM EDTA) supplemented with 10 μg/mL RNase (DNase-free (Roche)) and incubated at 65 °C overnight. .. DNA was purified using the NucleoSpin Gel and PCR Clean-up kit with Article Title: TERRA R-loops trigger a switch in telomere maintenance towards break-induced replication and PrimPol-dependent repair Article Snippet: Washed beads and input samples were resuspended in crosslink reversal buffer (20 mM Tris-HCl pH 8.0, 0.1% SDS, 0.1 M NaHCO3, 0.5 mM EDTA) supplemented with 10 μg/mL RNase (DNase-free (Roche)) and incubated at 65°C overnight. .. DNA was purified using the NucleoSpin Gel and PCR Clean-up kit with Polymerase Chain Reaction:Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA. Article Snippet: .. Samples were resuspended in two volumes of Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA Article Snippet: .. Samples were resuspended in two volumes of Article Title: TERRA R-loops trigger a switch in telomere maintenance towards break-induced replication and PRIMPOL-dependent repair Article Snippet: Washed beads and input samples were resuspended in crosslink reversal buffer (20 mM Tris-HCl pH 8.0, 0.1% SDS, 0.1 M NaHCO3, 0.5 mM EDTA) supplemented with 10 μg/mL RNase (DNase-free (Roche)) and incubated at 65 °C overnight. .. DNA was purified using the NucleoSpin Gel and PCR Clean-up kit with Article Title: Integrating high-throughput analysis to create an atlas of replication origins in Trypanosoma cruzi in the context of genome structure and variability. Article Snippet: .. ChIP and input samples were incubated for 9 h at 65°C for crosslink reversal and treated with RNase A (0.2 mg/mL) for 2 h at 37°C and then with proteinase K (0.2 mg/mL) for 2 h at 55°C, followed by DNA purification using a PCR Clean-Up Gel Extraction Kit (Macherey-Nagel) and Article Title: TERRA R-loops trigger a switch in telomere maintenance towards break-induced replication and PRIMPOL-dependent repair. Article Snippet: Washed beads and input samples were resuspended in crosslink reversal buffer (20 mM Tris-HCl pH 8.0, 0.1% SDS, 0.1 M NaHCO3, 0.5 mM EDTA) supplemented with 10 μg/mL RNase (DNase-free (Roche)) and incubated at 65 °C overnight. .. DNA was purified using the NucleoSpin Gel and PCR Clean-up kit with Article Title: TERRA R-loops trigger a switch in telomere maintenance towards break-induced replication and PrimPol-dependent repair Article Snippet: Washed beads and input samples were resuspended in crosslink reversal buffer (20 mM Tris-HCl pH 8.0, 0.1% SDS, 0.1 M NaHCO3, 0.5 mM EDTA) supplemented with 10 μg/mL RNase (DNase-free (Roche)) and incubated at 65°C overnight. .. DNA was purified using the NucleoSpin Gel and PCR Clean-up kit with Chromatin Immunoprecipitation:Article Title: Integrating high-throughput analysis to create an atlas of replication origins in Trypanosoma cruzi in the context of genome structure and variability. Article Snippet: .. ChIP and input samples were incubated for 9 h at 65°C for crosslink reversal and treated with RNase A (0.2 mg/mL) for 2 h at 37°C and then with proteinase K (0.2 mg/mL) for 2 h at 55°C, followed by DNA purification using a PCR Clean-Up Gel Extraction Kit (Macherey-Nagel) and Incubation:Article Title: Integrating high-throughput analysis to create an atlas of replication origins in Trypanosoma cruzi in the context of genome structure and variability. Article Snippet: .. ChIP and input samples were incubated for 9 h at 65°C for crosslink reversal and treated with RNase A (0.2 mg/mL) for 2 h at 37°C and then with proteinase K (0.2 mg/mL) for 2 h at 55°C, followed by DNA purification using a PCR Clean-Up Gel Extraction Kit (Macherey-Nagel) and DNA Purification:Article Title: Integrating high-throughput analysis to create an atlas of replication origins in Trypanosoma cruzi in the context of genome structure and variability. Article Snippet: .. ChIP and input samples were incubated for 9 h at 65°C for crosslink reversal and treated with RNase A (0.2 mg/mL) for 2 h at 37°C and then with proteinase K (0.2 mg/mL) for 2 h at 55°C, followed by DNA purification using a PCR Clean-Up Gel Extraction Kit (Macherey-Nagel) and Gel Extraction:Article Title: Integrating high-throughput analysis to create an atlas of replication origins in Trypanosoma cruzi in the context of genome structure and variability. Article Snippet: .. ChIP and input samples were incubated for 9 h at 65°C for crosslink reversal and treated with RNase A (0.2 mg/mL) for 2 h at 37°C and then with proteinase K (0.2 mg/mL) for 2 h at 55°C, followed by DNA purification using a PCR Clean-Up Gel Extraction Kit (Macherey-Nagel) and Binding Assay:Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA Article Snippet: BD DifcoTM Dehydrated Culture Media: LB Broth, Miller (Luria-Bertani) , BD , Cat.#244610. .. Article Title: KMT2C and KMT2D amplify GRHL2-driven enhancer activation Article Snippet: .. Samples were placed on magnet to transfer supernatant and cleaned up using |
![( A ) Coculture (1:1) of parental B16F10 cells expressing pBabe-Cerulean (blue) and B16F10 cells expressing GsdmD-C3 and mCherry (red) (see movie S1). ( B ) Times of individual cell shrinkage and cell membrane lysis, as in (A). Unpaired t test. ( C ) Apoptotic phase (shrinkage-lysis interval) from (B). One-way ANOVA. ( D ) Quantification of annexin V + SIINFEKL peptide–pulsed B16F10 [as in (B) and (C)] that remains fluorescent when coincubated with activated OT-I T cells at 4 hours. Two-way ANOVA. ( E ) Quantification of SIINFEKL peptide–pulsed B16F10 cell populations with ectopic caspase-3-activatable GSDMD (GD-C3) by fluorescence signal at 24 hours of coincubation with activated OT-I T cells. ( F ) As in (E) with Gsdme. [(E) and (F)] Paired t test. ( G ) Number of GCamp6s spikes observed from SIINFEKL peptide–pulsed Neuro2a cells, coincubated with activated OT-I T cells, 10:1 T cell–to–target cell ratio. Images were taken every 2 min for a duration of 3 hours immediately following the additional of OT-I T cells, and numbers of observable GCamp6s spikes were counted and plotted (see movies S2 to S4 and fig. S5C). Unpaired t test. ( H ) Design for (I) and (J). Created in BioRender; B. Liu (2026), https://BioRender.com/j67ipmw . ( I ) Bioluminescence signal intensity of Neuro2a in C57BL/6 animals ( n = 9 animals per group). Two-way ANOVA. ( J ) Kaplan-Meyer survival curves from C57BL/6 mice injected with Neuro2a cells ( n = 9 animals per group). ( K ) Design for (L). Created in BioRender; B. Liu (2026), https://BioRender.com/j67ipmw . ( L ) Bioluminescence signal intensity of Neuro2a in C57BL/6 animals (control, n = 9 animals; Gsdme −/− , n = 8 animals). ( M ) Kaplan-Meyer survival curves from C57BL/6 mice rechallenged with Neuro2a cells [ n = 5 animals per group; log-rank (Mantel-Cox) test]. ( N ) As in (M) with B16F10 [ n = 12 animals in naïve group, 14 animals in rechallenge group; log-rank (Mantel-Cox) test]. * P < 0.05, ** P < 0.01, and **** P < 0.0001. iv, intravenous; ip, intraperitoneal.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6084/pmc13626084/pmc13626084__sciadv.aeb1137-f3.jpg)
![PGCs are more sensitive to DNA double-strand breaks than somatic cells. (A) Cell viability curves after 24 h treatment with increasing concentrations of etoposide (ETP; x-axis shows log 10 [ETP]). PGC viability dropped sharply even at low ETP doses, whereas CEF cells were more tolerant (viability data are mean ± SD of triplicates). (B) Apoptosis detection in PGCs treated with low-dose ETP. The proportion of Annexin V + /PI + cells (late apoptosis) was significantly elevated even at 0.03 µM ETP. (C) Western blot analysis of γ-H 2 AX protein in PGCs treated with varying ETP concentrations (left panel; β-actin as loading control). The bar graph (right) shows the ratio of γ-H 2 AX to β-actin band intensity, with a marked increase at 3 µM ETP (p-values are indicated in the figure). (D) Quantification of γ-H 2 AX foci per cell in THP-1 cells, male PGCs, and female PGCs after exposure to X-ray doses of 0, 2, 4, 6, and 8 Gy. After 48 h recovery, γ-H 2 AX foci increased significantly with higher radiation in THP-1, male PGCs, and female PGCs. Female PGC data points are red squares; male PGCs are blue squares; THP-1 are black circles. (E) Cell-cycle distribution of male vs. female PGCs after DNA damage. PGCs were irradiated (2, 4, 6 Gy), cultured 48 h, and analyzed by flow cytometry for cell-cycle phase (propidium iodide staining). Stacked bars show the percentage of cells in G 0 G 1 , S, and G 2 /M phases in untreated vs. irradiated cells. After damage, female PGCs predominantly arrested in G 2 /M (increased G 2 fraction), whereas male PGCs accumulated in S phase. Statistical significance by one-way ANOVA (p-values are indicated in the figure).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1053/pmc13011053/pmc13011053__gr3.jpg)