DNA Purification:Article Title: System for the stabilization, conservation and storage of nucleic acid
Article Snippet: .. DNA purification according to the prior art 500 μl stabilized specimen is laced with 20 μl proteinase K 30 mg/ml and incubated at 50° C. for 10 min. 200 μl binding buffer B6 (Stratec Molecular) is added. .. The mixture is placed on a “spin filter” DNA binding filter (Stratec Molecular), followed by centrifugation at 10,000 g for 1 min. 500 μl wash buffer 1 (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min. 600 μl wash buffer 2 (Stratec Molecular) is added to the spin filter, followed by centrifugation at 10,000 g for 1 min. 600 μl wash buffer 1 (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min. Then dry centrifugation is performed at 10,000 g for 5 min. 100 μl elution buffer (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min.
Incubation:Article Title: System for the stabilization, conservation and storage of nucleic acid
Article Snippet: .. DNA purification according to the prior art 500 μl stabilized specimen is laced with 20 μl proteinase K 30 mg/ml and incubated at 50° C. for 10 min. 200 μl binding buffer B6 (Stratec Molecular) is added. .. The mixture is placed on a “spin filter” DNA binding filter (Stratec Molecular), followed by centrifugation at 10,000 g for 1 min. 500 μl wash buffer 1 (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min. 600 μl wash buffer 2 (Stratec Molecular) is added to the spin filter, followed by centrifugation at 10,000 g for 1 min. 600 μl wash buffer 1 (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min. Then dry centrifugation is performed at 10,000 g for 5 min. 100 μl elution buffer (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min.
Article Title: System for the stabilization, conservation and storage of nucleic acid
Article Snippet: .. After test subjects deposited sputum samples into the tubes, they were stabilized for 6 months and then isolated according to the following procedure: 500 μl stabilized specimen is laced with 20 μl proteinase K 30 mg/ml and incubated at 50° C. for 10 min. 200 μl binding buffer B6 (Stratec Molecular) is added. .. The mixture is placed onto a “spin filter” DNA binding filter (Stratec Molecular), followed by centrifugation at 10,000 g for 1 min. 500 μl wash buffer 1 (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min. 600 μl wash buffer 2 (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min. Then dry centrifugation is performed at 10,000 g for 5 min. 100 μl elution buffer (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min.
Binding Assay:Article Title: System for the stabilization, conservation and storage of nucleic acid
Article Snippet: .. DNA purification according to the prior art 500 μl stabilized specimen is laced with 20 μl proteinase K 30 mg/ml and incubated at 50° C. for 10 min. 200 μl binding buffer B6 (Stratec Molecular) is added. .. The mixture is placed on a “spin filter” DNA binding filter (Stratec Molecular), followed by centrifugation at 10,000 g for 1 min. 500 μl wash buffer 1 (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min. 600 μl wash buffer 2 (Stratec Molecular) is added to the spin filter, followed by centrifugation at 10,000 g for 1 min. 600 μl wash buffer 1 (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min. Then dry centrifugation is performed at 10,000 g for 5 min. 100 μl elution buffer (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min.
Article Title: System for the stabilization, conservation and storage of nucleic acid
Article Snippet: .. After test subjects deposited sputum samples into the tubes, they were stabilized for 6 months and then isolated according to the following procedure: 500 μl stabilized specimen is laced with 20 μl proteinase K 30 mg/ml and incubated at 50° C. for 10 min. 200 μl binding buffer B6 (Stratec Molecular) is added. .. The mixture is placed onto a “spin filter” DNA binding filter (Stratec Molecular), followed by centrifugation at 10,000 g for 1 min. 500 μl wash buffer 1 (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min. 600 μl wash buffer 2 (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min. Then dry centrifugation is performed at 10,000 g for 5 min. 100 μl elution buffer (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min.
Isolation:Article Title: System for the stabilization, conservation and storage of nucleic acid
Article Snippet: .. After test subjects deposited sputum samples into the tubes, they were stabilized for 6 months and then isolated according to the following procedure: 500 μl stabilized specimen is laced with 20 μl proteinase K 30 mg/ml and incubated at 50° C. for 10 min. 200 μl binding buffer B6 (Stratec Molecular) is added. .. The mixture is placed onto a “spin filter” DNA binding filter (Stratec Molecular), followed by centrifugation at 10,000 g for 1 min. 500 μl wash buffer 1 (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min. 600 μl wash buffer 2 (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min. Then dry centrifugation is performed at 10,000 g for 5 min. 100 μl elution buffer (Stratec Molecular) is placed on the spin filter, followed by centrifugation at 10,000 g for 1 min.
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