binary destination vectors (Addgene inc)
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binary destination vectors
Binary Destination Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/binary/Destination+Vector+(Plasmid+%23125754)/pm41934615-406-4-20
Average 96 stars, based on 273 article reviews
Binary Destination Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/binary/Destination+Vector+(Plasmid+%23125754)/pm41934615-406-4-20
Average 96 stars, based on 273 article reviews
binary destination vectors - by Bioz Stars,
2026-10
96/100 stars
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Plasmid Preparation:Article Title: Risk assessment of plant-to-bacterium transgene flow associated with novel small synthetic genome (minisynplastome) platforms for plastid genetic engineering Article Snippet: .. For construction of this Article Title: CRISPR/Cas9-Mediated Mutagenesis in Tomato Targeting the DE-ETIOLATED1 Gene Article Snippet: sgRNAs were cloned under the control of an AtU6-III promoter and upstream of the sgRNA scaffold (Addgene plasmid #46966 [34]) into the GoldenGate Level 1 acceptor vectors (pICH47732 and pICH47742). .. Subsequently, this transcriptional unit was assembled in a Article Title: Advancing knock-in approaches for robust genome editing in zebrafish Article Snippet: .. To incorporate this strategy, we further modified the Article Title: An epigenetic program dictates MYC amplification dynamics that promote transient, extrachromosomal, and inherited genomic events. Article Snippet: To create dCas9-KDM4 plasmids, the Invitrogen Gateway Cloning kit was used. .. The Amplification:Article Title: Risk assessment of plant-to-bacterium transgene flow associated with novel small synthetic genome (minisynplastome) platforms for plastid genetic engineering Article Snippet: .. For construction of this Clone Assay:Article Title: Risk assessment of plant-to-bacterium transgene flow associated with novel small synthetic genome (minisynplastome) platforms for plastid genetic engineering Article Snippet: .. For construction of this Article Title: Developmental Elimination of Electrical Synapses by UNC-51/UNC-76-Mediated Vesicular Transport Article Snippet: Plasmids were constructed using ClonExpress recombination technology (Vazyme, C112-01) or Gateway cloning technology (Invitrogen, 311791020). .. Gateway entry clones were generated using the backbone vector pCR8-JARID2 (Addgene, 114443), and Cloning:Article Title: Risk assessment of plant-to-bacterium transgene flow associated with novel small synthetic genome (minisynplastome) platforms for plastid genetic engineering Article Snippet: .. For construction of this Article Title: Antagonism between blue- and red-light signaling controls thallus flatness in Marchantia polymorpha. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MpPHOT peptide antibody Custom-made by Eurogentec, Belgium N/A Bacterial and virus strains Escherichia coli: strain DH5alpha N/A N/A Agrobacterium tumefaciens: strain GV3101 N/A N/A Escherichia coli: GreenGate Cloning System Lampropoulos et al.27 Addgene Kit #1000000036 Chemicals, peptides, and recombinant proteins Gamborg B5 Medium basal salt mixture Duchefa Cat#G0209 M51C medium Takenaka et al.28 N/A Plant agar Duchefa Cat#P1001 Hygromycin B Gibco Cat#10687010 Cefotaxime sodium Duchefa Cat#C0111 Chlorsulfuron Sigma Aldrich Cat# 34322 Sodium dichloroisocyanurate (NaDCC) Sigma Aldrich Cat#218928 Acetosyringone Sigma Aldrich Cat#D134406 Critical commercial assays QuikChange II site-directed mutagenesis kit Agilent Cat# 200523 Deposited data RNA Seq raw data- NCBI GEO repository https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE316508 GEO: GSE316508 Website for RNA Seq data exploration https://mplight.dolan.vbc.ac.at/ N/A Experimental models: organisms/strains Marchantia polymorpha: Tak-1 Ishizaki et al.29 N/A Marchantia polymorpha: Tak-2 Ishizaki et al.29 N/A Marchantia polymorpha: Tak-1 x Tak-2, F1 #1-10 This paper N/A Marchantia polymorpha: Mpphot-3 This paper N/A Marchantia polymorpha: Mpphot-4 This paper N/A Marchantia polymorpha: Mpphot-5 This paper N/A Marchantia polymorpha: proMpPHOT-MpPHOT/phot; #13 This paper N/A Marchantia polymorpha: proMpPHOT-MpPHOT/phot; #14 This paper N/A Arabidopsis thaliana: Col-0 ABRC N/A Arabidopsis thaliana: Col-0-gl1 John Christie, University of Glasgow N/A Arabidopsis thaliana: Atphot1-5 Atphot2-1 John Christie, University of Glasgow N/A Arabidopsis thaliana: proAtUBIQUITIN10: MpFKF / Atphot1 Atphot2 (41-1-5) This paper N/A Arabidopsis thaliana: proAtPHOT1: MpPHOT / Atphot1 Atphot2 (15-1-9) This paper N/A Arabidopsis thaliana: proAtPHOT2: MpPHOT / Atphot1 Atphot2 (16-5-4) This paper N/A Arabidopsis thaliana: proAtPHOT1: MpPHOT:VENUS / Atphot1 Atphot2 (17-6-3) This paper N/A Arabidopsis thaliana: proAtPHOT2: MpPHOT:VENUS / Atphot1 Atphot2 (18-5-5) This paper N/A Arabidopsis thaliana: proAtUBIQUITIN10:MpPHOT: VENUS / Atphot1 Atphot2 (19-1-8) This paper N/A (Continued on next page) ll Current Biology 36, 1–14.e1–e7, April 20, 2026 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Arabidopsis thaliana: proAtPHOT2:MpPHOT D904N: VENUS / Atphot1 Atphot2 (39-2-2) This paper N/A Arabidopsis thaliana: proAtUBIQUITIN10: MpPHOT D904N / Atphot1 Atphot2 (40-2-9) This paper N/A Marchantia polymorpha: proMpPHOT: MpPHOT:VENUS (GG7-15) This paper N/A Marchantia polymorpha: Mpphy-27 Streubel et al.16 N/A Marchantia polymorpha: Mpphy-38 Streubel et al.16 N/A Marchantia polymorpha: Mphy1-1 This paper N/A Marchantia polymorpha: Mphy1-2 This paper N/A Marchantia polymorpha: Mpbbx1-1 This paper N/A Marchantia polymorpha: Mpbbx1-2 This paper N/A Marchantia polymorpha: Mpbbx1-3 This paper N/A Marchantia polymorpha: Mpbbx5-1 This paper N/A Marchantia polymorpha: Mpbbx5-2 This paper N/A Marchantia polymorpha: Mpbbx5-3 This paper N/A Marchantia polymorpha: Mpbbx1-2 Mpbbx5-1 This paper N/A Marchantia polymorpha: Mpbbx1-2 Mpbbx5-3 This paper N/A Marchantia polymorpha: proMpEF1α:MpPHY #3 This paper N/A Marchantia polymorpha: proMpEF1α:MpPHY #6 This paper N/A Marchantia polymorpha: proMpEF1α:MpPHY #7 This paper N/A Marchantia polymorpha: proMpEF1α:MpPHY #10 This paper N/A Marchantia polymorpha: Mpbbx2-1 This paper N/A Marchantia polymorpha: Mpbbx2-2 This paper N/A Marchantia polymorpha: Mpbbx3-1 This paper N/A Marchantia polymorpha: Mpbbx3-2 This paper N/A Marchantia polymorpha: Mpbbx4-1 This paper N/A Marchantia polymorpha: Mpbbx4-2 This paper N/A Marchantia polymorpha: Mpbbx6-1 This paper N/A Marchantia polymorpha: Mpbbx6-2 This paper N/A Oligonucleotides See Table S1 This paper N/A Recombinant DNA OpenPlant toolkit vectors Sauret-Güeto et al.30 N/A GreenGate Cloning System: entry and Modification:Article Title: Advancing knock-in approaches for robust genome editing in zebrafish Article Snippet: .. To incorporate this strategy, we further modified the Subcloning:Article Title: Advancing knock-in approaches for robust genome editing in zebrafish Article Snippet: .. To incorporate this strategy, we further modified the Recombinant:Article Title: Antagonism between blue- and red-light signaling controls thallus flatness in Marchantia polymorpha. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MpPHOT peptide antibody Custom-made by Eurogentec, Belgium N/A Bacterial and virus strains Escherichia coli: strain DH5alpha N/A N/A Agrobacterium tumefaciens: strain GV3101 N/A N/A Escherichia coli: GreenGate Cloning System Lampropoulos et al.27 Addgene Kit #1000000036 Chemicals, peptides, and recombinant proteins Gamborg B5 Medium basal salt mixture Duchefa Cat#G0209 M51C medium Takenaka et al.28 N/A Plant agar Duchefa Cat#P1001 Hygromycin B Gibco Cat#10687010 Cefotaxime sodium Duchefa Cat#C0111 Chlorsulfuron Sigma Aldrich Cat# 34322 Sodium dichloroisocyanurate (NaDCC) Sigma Aldrich Cat#218928 Acetosyringone Sigma Aldrich Cat#D134406 Critical commercial assays QuikChange II site-directed mutagenesis kit Agilent Cat# 200523 Deposited data RNA Seq raw data- NCBI GEO repository https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE316508 GEO: GSE316508 Website for RNA Seq data exploration https://mplight.dolan.vbc.ac.at/ N/A Experimental models: organisms/strains Marchantia polymorpha: Tak-1 Ishizaki et al.29 N/A Marchantia polymorpha: Tak-2 Ishizaki et al.29 N/A Marchantia polymorpha: Tak-1 x Tak-2, F1 #1-10 This paper N/A Marchantia polymorpha: Mpphot-3 This paper N/A Marchantia polymorpha: Mpphot-4 This paper N/A Marchantia polymorpha: Mpphot-5 This paper N/A Marchantia polymorpha: proMpPHOT-MpPHOT/phot; #13 This paper N/A Marchantia polymorpha: proMpPHOT-MpPHOT/phot; #14 This paper N/A Arabidopsis thaliana: Col-0 ABRC N/A Arabidopsis thaliana: Col-0-gl1 John Christie, University of Glasgow N/A Arabidopsis thaliana: Atphot1-5 Atphot2-1 John Christie, University of Glasgow N/A Arabidopsis thaliana: proAtUBIQUITIN10: MpFKF / Atphot1 Atphot2 (41-1-5) This paper N/A Arabidopsis thaliana: proAtPHOT1: MpPHOT / Atphot1 Atphot2 (15-1-9) This paper N/A Arabidopsis thaliana: proAtPHOT2: MpPHOT / Atphot1 Atphot2 (16-5-4) This paper N/A Arabidopsis thaliana: proAtPHOT1: MpPHOT:VENUS / Atphot1 Atphot2 (17-6-3) This paper N/A Arabidopsis thaliana: proAtPHOT2: MpPHOT:VENUS / Atphot1 Atphot2 (18-5-5) This paper N/A Arabidopsis thaliana: proAtUBIQUITIN10:MpPHOT: VENUS / Atphot1 Atphot2 (19-1-8) This paper N/A (Continued on next page) ll Current Biology 36, 1–14.e1–e7, April 20, 2026 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Arabidopsis thaliana: proAtPHOT2:MpPHOT D904N: VENUS / Atphot1 Atphot2 (39-2-2) This paper N/A Arabidopsis thaliana: proAtUBIQUITIN10: MpPHOT D904N / Atphot1 Atphot2 (40-2-9) This paper N/A Marchantia polymorpha: proMpPHOT: MpPHOT:VENUS (GG7-15) This paper N/A Marchantia polymorpha: Mpphy-27 Streubel et al.16 N/A Marchantia polymorpha: Mpphy-38 Streubel et al.16 N/A Marchantia polymorpha: Mphy1-1 This paper N/A Marchantia polymorpha: Mphy1-2 This paper N/A Marchantia polymorpha: Mpbbx1-1 This paper N/A Marchantia polymorpha: Mpbbx1-2 This paper N/A Marchantia polymorpha: Mpbbx1-3 This paper N/A Marchantia polymorpha: Mpbbx5-1 This paper N/A Marchantia polymorpha: Mpbbx5-2 This paper N/A Marchantia polymorpha: Mpbbx5-3 This paper N/A Marchantia polymorpha: Mpbbx1-2 Mpbbx5-1 This paper N/A Marchantia polymorpha: Mpbbx1-2 Mpbbx5-3 This paper N/A Marchantia polymorpha: proMpEF1α:MpPHY #3 This paper N/A Marchantia polymorpha: proMpEF1α:MpPHY #6 This paper N/A Marchantia polymorpha: proMpEF1α:MpPHY #7 This paper N/A Marchantia polymorpha: proMpEF1α:MpPHY #10 This paper N/A Marchantia polymorpha: Mpbbx2-1 This paper N/A Marchantia polymorpha: Mpbbx2-2 This paper N/A Marchantia polymorpha: Mpbbx3-1 This paper N/A Marchantia polymorpha: Mpbbx3-2 This paper N/A Marchantia polymorpha: Mpbbx4-1 This paper N/A Marchantia polymorpha: Mpbbx4-2 This paper N/A Marchantia polymorpha: Mpbbx6-1 This paper N/A Marchantia polymorpha: Mpbbx6-2 This paper N/A Oligonucleotides See Table S1 This paper N/A Recombinant DNA OpenPlant toolkit vectors Sauret-Güeto et al.30 N/A GreenGate Cloning System: entry and Software:Article Title: Antagonism between blue- and red-light signaling controls thallus flatness in Marchantia polymorpha. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MpPHOT peptide antibody Custom-made by Eurogentec, Belgium N/A Bacterial and virus strains Escherichia coli: strain DH5alpha N/A N/A Agrobacterium tumefaciens: strain GV3101 N/A N/A Escherichia coli: GreenGate Cloning System Lampropoulos et al.27 Addgene Kit #1000000036 Chemicals, peptides, and recombinant proteins Gamborg B5 Medium basal salt mixture Duchefa Cat#G0209 M51C medium Takenaka et al.28 N/A Plant agar Duchefa Cat#P1001 Hygromycin B Gibco Cat#10687010 Cefotaxime sodium Duchefa Cat#C0111 Chlorsulfuron Sigma Aldrich Cat# 34322 Sodium dichloroisocyanurate (NaDCC) Sigma Aldrich Cat#218928 Acetosyringone Sigma Aldrich Cat#D134406 Critical commercial assays QuikChange II site-directed mutagenesis kit Agilent Cat# 200523 Deposited data RNA Seq raw data- NCBI GEO repository https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE316508 GEO: GSE316508 Website for RNA Seq data exploration https://mplight.dolan.vbc.ac.at/ N/A Experimental models: organisms/strains Marchantia polymorpha: Tak-1 Ishizaki et al.29 N/A Marchantia polymorpha: Tak-2 Ishizaki et al.29 N/A Marchantia polymorpha: Tak-1 x Tak-2, F1 #1-10 This paper N/A Marchantia polymorpha: Mpphot-3 This paper N/A Marchantia polymorpha: Mpphot-4 This paper N/A Marchantia polymorpha: Mpphot-5 This paper N/A Marchantia polymorpha: proMpPHOT-MpPHOT/phot; #13 This paper N/A Marchantia polymorpha: proMpPHOT-MpPHOT/phot; #14 This paper N/A Arabidopsis thaliana: Col-0 ABRC N/A Arabidopsis thaliana: Col-0-gl1 John Christie, University of Glasgow N/A Arabidopsis thaliana: Atphot1-5 Atphot2-1 John Christie, University of Glasgow N/A Arabidopsis thaliana: proAtUBIQUITIN10: MpFKF / Atphot1 Atphot2 (41-1-5) This paper N/A Arabidopsis thaliana: proAtPHOT1: MpPHOT / Atphot1 Atphot2 (15-1-9) This paper N/A Arabidopsis thaliana: proAtPHOT2: MpPHOT / Atphot1 Atphot2 (16-5-4) This paper N/A Arabidopsis thaliana: proAtPHOT1: MpPHOT:VENUS / Atphot1 Atphot2 (17-6-3) This paper N/A Arabidopsis thaliana: proAtPHOT2: MpPHOT:VENUS / Atphot1 Atphot2 (18-5-5) This paper N/A Arabidopsis thaliana: proAtUBIQUITIN10:MpPHOT: VENUS / Atphot1 Atphot2 (19-1-8) This paper N/A (Continued on next page) ll Current Biology 36, 1–14.e1–e7, April 20, 2026 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Arabidopsis thaliana: proAtPHOT2:MpPHOT D904N: VENUS / Atphot1 Atphot2 (39-2-2) This paper N/A Arabidopsis thaliana: proAtUBIQUITIN10: MpPHOT D904N / Atphot1 Atphot2 (40-2-9) This paper N/A Marchantia polymorpha: proMpPHOT: MpPHOT:VENUS (GG7-15) This paper N/A Marchantia polymorpha: Mpphy-27 Streubel et al.16 N/A Marchantia polymorpha: Mpphy-38 Streubel et al.16 N/A Marchantia polymorpha: Mphy1-1 This paper N/A Marchantia polymorpha: Mphy1-2 This paper N/A Marchantia polymorpha: Mpbbx1-1 This paper N/A Marchantia polymorpha: Mpbbx1-2 This paper N/A Marchantia polymorpha: Mpbbx1-3 This paper N/A Marchantia polymorpha: Mpbbx5-1 This paper N/A Marchantia polymorpha: Mpbbx5-2 This paper N/A Marchantia polymorpha: Mpbbx5-3 This paper N/A Marchantia polymorpha: Mpbbx1-2 Mpbbx5-1 This paper N/A Marchantia polymorpha: Mpbbx1-2 Mpbbx5-3 This paper N/A Marchantia polymorpha: proMpEF1α:MpPHY #3 This paper N/A Marchantia polymorpha: proMpEF1α:MpPHY #6 This paper N/A Marchantia polymorpha: proMpEF1α:MpPHY #7 This paper N/A Marchantia polymorpha: proMpEF1α:MpPHY #10 This paper N/A Marchantia polymorpha: Mpbbx2-1 This paper N/A Marchantia polymorpha: Mpbbx2-2 This paper N/A Marchantia polymorpha: Mpbbx3-1 This paper N/A Marchantia polymorpha: Mpbbx3-2 This paper N/A Marchantia polymorpha: Mpbbx4-1 This paper N/A Marchantia polymorpha: Mpbbx4-2 This paper N/A Marchantia polymorpha: Mpbbx6-1 This paper N/A Marchantia polymorpha: Mpbbx6-2 This paper N/A Oligonucleotides See Table S1 This paper N/A Recombinant DNA OpenPlant toolkit vectors Sauret-Güeto et al.30 N/A GreenGate Cloning System: entry and Generated:Article Title: Developmental Elimination of Electrical Synapses by UNC-51/UNC-76-Mediated Vesicular Transport Article Snippet: Plasmids were constructed using ClonExpress recombination technology (Vazyme, C112-01) or Gateway cloning technology (Invitrogen, 311791020). .. Gateway entry clones were generated using the backbone vector pCR8-JARID2 (Addgene, 114443), and Derivative Assay:Article Title: Developmental Elimination of Electrical Synapses by UNC-51/UNC-76-Mediated Vesicular Transport Article Snippet: Plasmids were constructed using ClonExpress recombination technology (Vazyme, C112-01) or Gateway cloning technology (Invitrogen, 311791020). .. Gateway entry clones were generated using the backbone vector pCR8-JARID2 (Addgene, 114443), and |