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FUJIFILM beta-glucan test wt
Study design and inclusion criteria. Abbreviations: BDG: 1.3 <t>beta-</t> d -glucan; IAC: intra-abdominal infection; IAI: intra-abdominal infection. Risk factors according to [ – ], Peritonitis score according to
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1) Product Images from "Combination of serum and peritoneal 1.3-beta- d -glucan can rule out intra-abdominal candidiasis in surgical critically ill patients: a multicenter prospective study"

Article Title: Combination of serum and peritoneal 1.3-beta- d -glucan can rule out intra-abdominal candidiasis in surgical critically ill patients: a multicenter prospective study

Journal: Critical Care

doi: 10.1186/s13054-023-04761-7

Study design and inclusion criteria. Abbreviations: BDG: 1.3 beta- d -glucan; IAC: intra-abdominal infection; IAI: intra-abdominal infection. Risk factors according to [ – ], Peritonitis score according to
Figure Legend Snippet: Study design and inclusion criteria. Abbreviations: BDG: 1.3 beta- d -glucan; IAC: intra-abdominal infection; IAI: intra-abdominal infection. Risk factors according to [ – ], Peritonitis score according to

Techniques Used: Infection

Serum and peritoneal 1.3 beta- d -glucan concentrations in IAC patients versus non-IAC. A Box and whiskers with median, 10 and 90% percentile of peritoneal BDG between confirmed IAC and non-IAC patients (Wako ® beta-glucan test, Fujifilm Wako Chemicals Europe, Neuss, Germany). Dotted line represents the threshold of 45 pg/ml. B Box and whiskers with median, 10 and 90% percentile of serum BDG measured with the Wako ® beta-glucan test (Fujifilm Wako Chemicals Europe, Neuss, Germany) at Day 1 between confirmed IAC and non-IAC patients. Dotted line represents the threshold of 3.3 pg/ml (WT). C Box and whiskers with median, 10 and 90% percentile of serum BDG measured with the Fungitell ® beta- d -glucan assay (FA, Associate of Cape Cod, East Falmouth, Inc., United States of America) at Day 1 between confirmed IAC and non-IAC patients. Dotted line represents the threshold of 80 pg/ml (FA). Abbreviations: IAC: intra-abdominal candidiasis; BDG: 1.3 beta- d -glucan; WT: Wako ® beta-glucan test (Fujifilm Wako Chemicals Europe, Neuss, Germany); FA: Fungitell ® beta- d -glucan assay (Associate of Cape Cod, East Falmouth, Inc., United States of America)
Figure Legend Snippet: Serum and peritoneal 1.3 beta- d -glucan concentrations in IAC patients versus non-IAC. A Box and whiskers with median, 10 and 90% percentile of peritoneal BDG between confirmed IAC and non-IAC patients (Wako ® beta-glucan test, Fujifilm Wako Chemicals Europe, Neuss, Germany). Dotted line represents the threshold of 45 pg/ml. B Box and whiskers with median, 10 and 90% percentile of serum BDG measured with the Wako ® beta-glucan test (Fujifilm Wako Chemicals Europe, Neuss, Germany) at Day 1 between confirmed IAC and non-IAC patients. Dotted line represents the threshold of 3.3 pg/ml (WT). C Box and whiskers with median, 10 and 90% percentile of serum BDG measured with the Fungitell ® beta- d -glucan assay (FA, Associate of Cape Cod, East Falmouth, Inc., United States of America) at Day 1 between confirmed IAC and non-IAC patients. Dotted line represents the threshold of 80 pg/ml (FA). Abbreviations: IAC: intra-abdominal candidiasis; BDG: 1.3 beta- d -glucan; WT: Wako ® beta-glucan test (Fujifilm Wako Chemicals Europe, Neuss, Germany); FA: Fungitell ® beta- d -glucan assay (Associate of Cape Cod, East Falmouth, Inc., United States of America)

Techniques Used:

Peritoneal and serum 1.3 beta- d -glucan to rule out intra-abdominal candidiasis in secondary peritonitis. Receiver operating characteristic curve of peritoneal and serum 1.3 beta- d -glucan for identification of intra-abdominal candidiasis ( A —peritoneal BDG (n = 196)/ B —serum BDG at Day 1 according to the test used (Wako test ® N = 42 and Fungitell ® beta- d -glucan assay N = 140). Abbreviations: FA: Fungitell ® beta- d -glucan assay (Associate of Cape Cod, East Falmouth, Inc., United States of America); pBDG: peritoneal 1.3 beta- d -glucan; sBDG: serum 1.3 beta- d -glucan; WT: Wako ® beta-glucan test (Fujifilm Wako Chemicals Europe, Neuss, Germany)
Figure Legend Snippet: Peritoneal and serum 1.3 beta- d -glucan to rule out intra-abdominal candidiasis in secondary peritonitis. Receiver operating characteristic curve of peritoneal and serum 1.3 beta- d -glucan for identification of intra-abdominal candidiasis ( A —peritoneal BDG (n = 196)/ B —serum BDG at Day 1 according to the test used (Wako test ® N = 42 and Fungitell ® beta- d -glucan assay N = 140). Abbreviations: FA: Fungitell ® beta- d -glucan assay (Associate of Cape Cod, East Falmouth, Inc., United States of America); pBDG: peritoneal 1.3 beta- d -glucan; sBDG: serum 1.3 beta- d -glucan; WT: Wako ® beta-glucan test (Fujifilm Wako Chemicals Europe, Neuss, Germany)

Techniques Used:

Related Articles

Infection:

Article Title: Effectiveness of pulse methylprednisolone in patients with non-human immunodeficiency virus pneumocystis pneumonia: a multicentre, retrospective registry-based cohort study
Article Snippet: Serum concentrations of β-D-glucan were assessed using either the β-D-glucan test kit (Wako Pure Chemical Industries, Osaka, Japan) or the FUNGITEC G test MKII (Nissui Pharmaceutical, Tokyo, Japan).

Article Title: Comparison of the Clinical Characteristics of Pneumocystis Pneumonia between Patients with Rheumatoid Arthritis Being Treated with Biologics and Those Being Treated without Biologics
Article Snippet: The manufacturer suggested that the upper normal limit for serum β -D-glucan was 11.3 pg/mL (Wako Pure Chemical Industries, Tokyo, Japan), but we defined 31.1 pg/mL as a cut-off value in this study to exclude Pneumocystis jirovecii colonization, based on a previous study [ ].

Article Title: Combination of serum and peritoneal 1.3-beta- d -glucan can rule out intra-abdominal candidiasis in surgical critically ill patients: a multicenter prospective study
Article Snippet: Wako ® beta-glucan test (WT, Fujifilm Wako Chemicals Europe, Neuss, Germany) was used for pBDG measurements.

Article Title: Clinical evaluation of two different (1,3)-ß-d-glucan assays for diagnosis of invasive fungal diseases: A retrospective cohort study.
Article Snippet: AUC area under the ROC curve BDG (1,3)-ß-D-glucan CI confidence interval EORTC/MSG European Organization for Research and Treatment of Cancer and the Mycoses Study Group Education and Research Consortium FA Fungitell Assay ® (Associates of Cape Cod Inc., USA) GT β-Glucan Test (FUJIFILM Wako Chemicals Europe GmbH, Germany) ICU intensive care unit IFD invasive fungal disease JUH Jena University Hospital NPV negative predictive value OR odds ratio PPV positive predictive value ROC receiver operating characteristics

Article Title: Clinical evaluation of two different (1,3)-ß-d-glucan assays for diagnosis of invasive fungal diseases: A retrospective cohort study.
Article Snippet: JH received beta-D-glucan kits free of charge for other studies from Associates of Cape Cod, Inc. and from FUJIFILM Wako Chemicals Europe GmbH.

Article Title: Pneumocystis jirovecii Pneumonia after Heart Transplantation: Two Case Reports and a Review of the Literature.
Article Snippet: Quantitative serum Serum Beta-D-Glucan (BDG) was performed (ß-Glucan Test on Toxinometer MT-6500, FUJIFILM Wako Pure Chemical Corporation, Japan) and resulted positive (207.7 pg/dL, reference value < 7pg/dL).

Article Title: Detection of Candida albicans in the peripheral blood smear of a diffuse large B cell lymphoma patient.
Article Snippet: On day 25, β-D-glucan had increased to 461.0 pg/mL (β-D-glucan test kit from Wako Pure Chemical Industries, Japan).

Article Title: Combination of serum and peritoneal 1.3-beta-D-glucan can rule out intra-abdominal candidiasis in surgical critically ill patients: a multicenter prospective study.
Article Snippet: Wako® beta‐glucan test (WT, Fujifilm Wako Chemicals Europe, Neuss, Germany) was used for pBDG measurements.



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TAF15 facilitates M2-like polarization of macrophages, enhancing TGFBI secretion and promoting the progression of ICC. (A) Reactome enrichment analysis of DEGs is depicted, with the top 10 enriched terms displayed. (B) Expression heatmaps of extracellular matrix organization. (C) Upper: a UMAP plot is colored by different clusters. Lower: a dot plot represents the mean expression of TGFBI across various clusters. (D) Western blot analysis examines the expression of TGFBI in different groups. (H) The colony formation ability of ICC cells treated with 0, 5, and 10 μg/ml recombinant TGFBI protein is assessed. (F–I) ELISA analysis measures the levels of TGFBI in the different CM of TAMs. Data are presented as mean ± SD, one-way ANOVA was performed for statistical analysis, n = 3. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. CM, conditional media; DEGs, differentially expressed genes; ICC, intrahepatic cholangiocarcinoma; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

Journal: JHEP Reports

Article Title: TAF15 in tumor-associated macrophages enhances protumorigenic polarization and promotes cholangiocarcinoma progression

doi: 10.1016/j.jhepr.2025.101545

Figure Lengend Snippet: TAF15 facilitates M2-like polarization of macrophages, enhancing TGFBI secretion and promoting the progression of ICC. (A) Reactome enrichment analysis of DEGs is depicted, with the top 10 enriched terms displayed. (B) Expression heatmaps of extracellular matrix organization. (C) Upper: a UMAP plot is colored by different clusters. Lower: a dot plot represents the mean expression of TGFBI across various clusters. (D) Western blot analysis examines the expression of TGFBI in different groups. (H) The colony formation ability of ICC cells treated with 0, 5, and 10 μg/ml recombinant TGFBI protein is assessed. (F–I) ELISA analysis measures the levels of TGFBI in the different CM of TAMs. Data are presented as mean ± SD, one-way ANOVA was performed for statistical analysis, n = 3. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. CM, conditional media; DEGs, differentially expressed genes; ICC, intrahepatic cholangiocarcinoma; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

Article Snippet: The transforming growth factor-beta induced (TGFBI) ELISA test kit was purchased from CUSABIO (CSB-EL023450MO, CSB-E16665h, Wuhan, China).

Techniques: Expressing, Western Blot, Recombinant, Enzyme-linked Immunosorbent Assay, Binding Assay

M2pepLNP-siTaf15 shows promise in targeting macrophages for the treatment of ICC. (A,B) In vivo and in vitro small animal imaging was conducted using free IR780 and M2pepLNP/IR780 in ICC mice via the tail vein. (C) Self-luminescence (Dil) with F4/80 multicolor immunofluorescence. (D,E) Western blot and QPCR analysis of Taf15 in BMDM after transfection with small interference for 48 h. (F) A schematic diagram illustrates the encapsulation of siTaf15 by M2pep-liposomes. (G) A schematic diagram depicts the treatment regimen of ICC mice with liposome materials. (H) Differential morphology of the liver is observed after treatment with M2pepLNP coated with siNc and siTaf15. (I) Weight of the liver is measured following treatment with M2LNP coated with siNc and siTaf15. (J) H&E and IHC analyses are conducted for CK19 and TAF15 in liver tissues treated with M2pepLNP-siNc and M2pepLNP-siTaf15. (K) H&E analysis of heart, lung, spleen, kidney in mice treated with M2pepLNP-siNc and M2pepLNP-siTaf15. (L) Flow cytometry analysis the expression of CD206 and CD86 of macrophage in mice liver. (M) ELISA analysis measures the levels of TGFBI in eyeball blood and liver tissue from mice. Data are presented as mean ± SD, unpaired two-tailed Student’s t test. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. BMDM, bone marrow-derived macrophages; ICC, intrahepatic cholangiocarcinoma; IHC, immunohistochemistry; QPCR, quantitative PCR; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

Journal: JHEP Reports

Article Title: TAF15 in tumor-associated macrophages enhances protumorigenic polarization and promotes cholangiocarcinoma progression

doi: 10.1016/j.jhepr.2025.101545

Figure Lengend Snippet: M2pepLNP-siTaf15 shows promise in targeting macrophages for the treatment of ICC. (A,B) In vivo and in vitro small animal imaging was conducted using free IR780 and M2pepLNP/IR780 in ICC mice via the tail vein. (C) Self-luminescence (Dil) with F4/80 multicolor immunofluorescence. (D,E) Western blot and QPCR analysis of Taf15 in BMDM after transfection with small interference for 48 h. (F) A schematic diagram illustrates the encapsulation of siTaf15 by M2pep-liposomes. (G) A schematic diagram depicts the treatment regimen of ICC mice with liposome materials. (H) Differential morphology of the liver is observed after treatment with M2pepLNP coated with siNc and siTaf15. (I) Weight of the liver is measured following treatment with M2LNP coated with siNc and siTaf15. (J) H&E and IHC analyses are conducted for CK19 and TAF15 in liver tissues treated with M2pepLNP-siNc and M2pepLNP-siTaf15. (K) H&E analysis of heart, lung, spleen, kidney in mice treated with M2pepLNP-siNc and M2pepLNP-siTaf15. (L) Flow cytometry analysis the expression of CD206 and CD86 of macrophage in mice liver. (M) ELISA analysis measures the levels of TGFBI in eyeball blood and liver tissue from mice. Data are presented as mean ± SD, unpaired two-tailed Student’s t test. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. BMDM, bone marrow-derived macrophages; ICC, intrahepatic cholangiocarcinoma; IHC, immunohistochemistry; QPCR, quantitative PCR; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

Article Snippet: The transforming growth factor-beta induced (TGFBI) ELISA test kit was purchased from CUSABIO (CSB-EL023450MO, CSB-E16665h, Wuhan, China).

Techniques: In Vivo, In Vitro, Imaging, Immunofluorescence, Western Blot, Transfection, Encapsulation, Liposomes, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Derivative Assay, Immunohistochemistry, Real-time Polymerase Chain Reaction, Binding Assay