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basic nucleofector tm kit for primary mammalian neurons  (Lonza)


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    Structured Review

    Lonza basic nucleofector tm kit for primary mammalian neurons

    Basic Nucleofector Tm Kit For Primary Mammalian Neurons, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+nucleofector+tm+kit/basic+nucleofector+kit+for+primary+mammalian+neurons/pmc08065343-44-0-9
    Average 90 stars, based on 1 article reviews
    basic nucleofector tm kit for primary mammalian neurons - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Protocol for electron microscopy ultrastructural localization of the fusogenic lipid phosphatidic acid on plasma membrane sheets from chromaffin cells"

    Article Title: Protocol for electron microscopy ultrastructural localization of the fusogenic lipid phosphatidic acid on plasma membrane sheets from chromaffin cells

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2021.100464


    Figure Legend Snippet:

    Techniques Used: Recombinant, Clinical Proteomics, Modification, Electron Microscopy, Plasmid Preparation, Software

    Related Articles

    Transfection:

    Article Title: Loss of SORCS2 is Associated with Neuronal DNA Double-Strand Breaks.
    Article Snippet: .. Cells were dissociated using TrypLE (12605036, Thermo Scientific), counted and 2 × 106 cells were transfected using the Basic Nucleofector kit for primary neurons (VAPI-1003, Lonza) and the D-33 programme on the Amaxa Nucleofector II B device (Amaxa Biosystems). .. 500 μl of pre-warmed RPMI media (BE12-752F, Lonza) was added following nucleofection.

    Article Title: Roles of SNORD115 and SNORD116 ncRNA clusters during neuronal differentiation
    Article Snippet: To delete SNORD115 and SNORD116 clusters, Alt-R CRISPR-Cas9 system from IDT was used: two crRNA:tracrRNA guides (for sequences see Supplementary Data ) for upstream and downstream cleavage complexed with Alt-R Cas9 nuclease 3NLS (ThermoFisher Scientific, 1074182) were prepared using following protocol: 0.5 μl crRNA-U [200 μM], 0.5 μl crRNA-D [200 μM] and 1 μl tracrRNA-ATTO [200 μM] were annealed in the PCR machine: 5 min at 95 °C, ramp −0.1 °C/sec to 25 °C. .. To deliver preassembled complexes and ssODN as a repair template, LUHMES cells were transfected by Nucleofection in a Nucleofector II device (Lonza) using a Basic Nucleofector Kit for primary neurons (Lonza, VAPI-1003) as described . ..

    other:

    Article Title: Compartment-specific regulation of Na V 1.7 in sensory neurons after acute exposure to TNF-α.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Tetrodotoxin Calbiochem 554412 TNF-a Invitrogen RRTNFAI SB203580 Merck Millipore 152121-47-6 SC-514 Tocris 3318 JF650X-HaloTag ligand HHMI Janelia Research Campus N/A JF549i-HaloTag ligand HHMI Janelia Research Campus N/A JF635i-HaloTag ligand HHMI Janelia Research Campus N/A Lipofectamine 3000 Invitrogen L3000001 TransIT-LT1 Transfection reagent Mirus MIR 2300 Basic Nucleofector Kit for Primary Mammalian Neurons Lonza V4XP-3032 Critical commercial assays Rat TNF-a ELISA MAXTM Deluxe Set BioLegend 438204 Experimental models: Cell lines ND7/23 Sigma 92090903 HEK293 Sigma 85120602 Experimental models: Organisms/strains Sprague-Dawley rat pups Envigo N/A Nav1.8 KO mice Dr. John Wood, UCL N/A Recombinant DNA hNav1.7 This Paper N/A hNav1.7 Septuple Mutant This Paper N/A hNav1.7 Quadruple Mutant This Paper N/A hNav1.7 Triple Mutant This Paper N/A hNav1.7 T103A This Paper N/A hNav1.7 S110A This Paper N/A hNav1.7 S113A This Paper N/A hNav1.7-Nterminus-Halo This Paper N/A Halo-Nav1.7 This Paper N/A Halo-Nav1.7 S110A This Paper N/A Software and algorithms PatchMaster Next HEKA Elektronik https://www.heka.com/downloads/downloads_main.html#down_ patchmaster_next Python Python Software Foundation https://www.python.org Prism GraphPad Software, Inc https://www.graphpad.com/ Andor Fusion Oxford Instruments N/A Analyzer Sophion https://sophion.com/products/high-throughput-screening-on-qube/ ViewPoint Sophion https://sophion.com/products/high-throughput-screening-on-qube/ pClamp Molecular Devices https://www.moleculardevices.com/products/axon-patch-clamp- system/acquisition-and-analysis-software/pclamp-software-suite Adobe Illustrator Adobe N/A (Continued on next page) Cell Reports 43, 113685, February 27, 2024 17

    Article Title: Involvement of advillin in somatosensory neuron subtype-specific axon regeneration and neuropathic pain
    Article Snippet: Nucleofector kits VPI-1003 (Lonza, MD, USA) and Nucleofector II apparatus (Lonza, MD, USA) were used.

    Plasmid Preparation:

    Article Title: STIM1 and ORAI1 form a novel cold transduction mechanism in sensory and sympathetic neurons
    Article Snippet: The siRNA was resuspended in RNase‐free siRNA buffer containing 60 mM KCl, 6 mM HEPES‐pH 7.5 and 0.2 mM MgCl 2 to produce 20 μM stock and was stored at −20°C. siRNA concentration was verified using a Nanodrop ND‐1000 UV spectrophotometer (Sinica). .. Superior cervical ganglion (SCG) neurons were enzymatically dissociated as described previously (Eickholt et al , ) and resuspended in supplemented Nucleofector Solution for primary mammalian neurons (Lonza, Catalogue #: VSPI‐1003) containing 300 nM siRNA and 0.4 μg mCherry plasmid at room temperature in aliquots of 20,000–50,000 neurons each and placed in small cell number (SCN) certified cuvettes. .. Neurons were electroporated using SCN Basic Neuron Programme 6 of a Nucleofector II machine (Amaxa Scientific) as per the manufacturer's instructions.

    Article Title: STIM1 and ORAI1 form a novel cold transduction mechanism in sensory and sympathetic neurons.
    Article Snippet: The siRNA was resuspended in RNase-free siRNA buffer containing 60 mM KCl, 6 mM HEPES-pH 7.5 and 0.2 mM MgCl2 to produce 20 lM stock and was stored at 20°C. siRNA concentration was verified using a Nanodrop ND-1000 UV spectrophotometer (Sinica). .. Superior cervical ganglion (SCG) neurons were enzymatically dissociated as described previously (Eickholt et al, 2007) and resuspended in supplemented Nucleofector Solution for primary mammalian neurons (Lonza, Catalogue #: VSPI-1003) containing 300 nM siRNA and 0.4 lg mCherry plasmid at room temperature in aliquots of 20,000–50,000 neurons each and placed in small cell number (SCN) certified cuvettes. .. Neurons were electroporated using SCN Basic Neuron Programme 6 of a Nucleofector II machine (Amaxa Scientific) as per the manufacturer’s instructions.



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Protocol for electron microscopy ultrastructural localization of the fusogenic lipid phosphatidic acid on plasma membrane sheets from chromaffin cells

    doi: 10.1016/j.xpro.2021.100464

    Figure Lengend Snippet:

    Article Snippet: Basic Nucleofector TM Kit for Primary Mammalian Neurons , Lonza , Cat#VVPI-1003.

    Techniques: Recombinant, Clinical Proteomics, Modification, Electron Microscopy, Plasmid Preparation, Software